primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnM-CAU_p1	trnG-GCC-trnM-CAU	1	GCGAGCGTAGTTCAATGGTA	CTAGAGCGGAGTAGAGCAGT	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.120934	12140	12609	595-622	1.000000	83.8
trnG-GCC-trnM-CAU_p2	trnG-GCC-trnM-CAU	2	CGTAGCGAGCGTAGTTCAAT	CTAGAGCGGAGTAGAGCAGT	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.214378	12140	12609	599-626	1.000000	83.0
trnG-GCC-trnM-CAU_p3	trnG-GCC-trnM-CAU	3	TAGCGAGCGTAGTTCAATGG	CTAGAGCGGAGTAGAGCAGT	20	20	50.0	55.0	57.8	58.0	0.0	0.0	0.242007	12140	12609	597-624	1.000000	82.8
trnG-GCC-trnM-CAU_p4	trnG-GCC-trnM-CAU	4	GCGAGCGTAGTTCAATGGTA	CGAGGCTCATAACCTTGAGG	20	20	50.0	55.0	58.1	57.8	0.0	16.0	0.313942	12140	12609	564-591	1.000000	82.5
trnG-GCC-trnM-CAU_p5	trnG-GCC-trnM-CAU	5	CGACCCAAGTGGATATACGC	CTAGAGCGGAGTAGAGCAGT	20	20	55.0	55.0	58.2	58.0	0.0	0.0	0.258637	12140	12609	633-660	1.000000	82.4
trnY-GUA-trnD-GUC_p1	trnY-GUA-trnD-GUC	1	AATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	58.0	58.5	0.0	7.1	0.521454	15622	15998	487-502	1.000000	79.1
trnY-GUA-trnD-GUC_p2	trnY-GUA-trnD-GUC	2	AATTCTTCCTGGGTCGATGC	ACTCTAGTTCGGGACTGACG	20	20	50.0	55.0	58.0	58.5	0.0	0.0	0.593056	15622	15998	540-555	1.000000	78.2
trnY-GUA-trnD-GUC_p3	trnY-GUA-trnD-GUC	3	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	20	20	55.0	50.0	58.4	58.5	0.0	7.1	0.828828	15622	15998	454-469	1.000000	76.8
trnY-GUA-trnD-GUC_p4	trnY-GUA-trnD-GUC	4	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.6	58.5	0.0	7.1	0.852505	15622	15998	453-468	1.000000	76.7
trnY-GUA-trnD-GUC_p5	trnY-GUA-trnD-GUC	5	GGGGACGGACTGTAAATTCG	ACTCTAGTTCGGGACTGACG	20	20	55.0	55.0	58.4	58.5	0.0	0.0	0.900429	15622	15998	507-522	1.000000	75.9
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	AAAGGATTTGCAGTCCCCTG	ATTCCCTCGTTTCCATTCCG	20	20	50.0	50.0	58.1	58.0	14.6	0.0	0.109519	18238	19398	1203-1230	1.000000	81.4
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	20	20	50.0	50.0	58.1	57.8	14.6	0.0	0.250044	18238	19398	1210-1237	1.000000	80.3
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	18238	19398	1218-1245	1.000000	79.1
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	18238	19398	1217-1244	1.000000	79.1
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGGCTT	20	20	50.0	45.0	58.1	57.6	14.6	13.0	0.442790	18238	19398	1282-1309	1.000000	78.6
atpF_p1	atpF	1	GGGCGAGCAAAGTAAGTGAT	TTCGGAAGCAAGGGGAAAAA	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.335662	32544	33944	1523-1538	1.000000	78.4
atpF_p2	atpF	2	GGGCGAGCAAAGTAAGTGAT	TTTCGGAAGCAAGGGGAAAA	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.335662	32544	33944	1524-1539	1.000000	78.4
atpF_p3	atpF	3	GGGCGAGCAAAGTAAGTGAT	TTTTCGGAAGCAAGGGGAAA	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.335662	32544	33944	1525-1540	1.000000	78.4
atpF_p4	atpF	4	GGGCGAGCAAAGTAAGTGAT	TTTTTCGGAAGCAAGGGGAA	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.335662	32544	33944	1526-1541	1.000000	78.4
atpF_p5	atpF	5	AAAGGGCGAGCAAAGTAAGT	TTCGGAAGCAAGGGGAAAAA	20	20	45.0	45.0	57.7	57.9	0.0	0.0	0.346673	32544	33944	1526-1541	1.000000	78.3
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TCTCCTGAGGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.212764	43689	44251	746-746	1.000000	79.0
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTCAA	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.254079	43689	44251	696-696	1.000000	78.8
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TCTCCTGAGGTTGTCGGAAT	AGTTCCAATGCTACGCCTTC	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.322349	43689	44251	698-698	1.000000	78.2
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTCTCCTGAGGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.3	58.2	0.0	0.0	0.439292	43689	44251	747-747	1.000000	77.2
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTTCTCCTGAGGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.3	58.2	0.0	0.0	0.439292	43689	44251	748-748	1.000000	77.2
trnL-UAA_p1	trnL-UAA	1	TGCTAGACTACACTACGTCGA	TCTCTTCGCACTCCTTTTGG	21	20	47.6	50.0	57.8	57.8	0.0	0.0	1.406464	46417	47049	850-855	1.000000	69.2
trnL-UAA_p2	trnL-UAA	2	TGCTAGACTACACTACGTCG	TCTCTTCGCACTCCTTTTGG	20	20	50.0	50.0	56.3	57.8	0.0	0.0	1.912223	46417	47049	850-855	1.000000	65.2
trnL-UAA_p3	trnL-UAA	3	GCTAGACTACACTACGTCGAG	TCTCTTCGCACTCCTTTTGG	21	20	52.4	50.0	57.2	57.8	0.0	0.0	2.009656	46417	47049	849-854	1.000000	64.4
trnL-UAA_p4	trnL-UAA	4	TTGCTAGACTACACTACGTCG	TCTCTTCGCACTCCTTTTGG	21	20	47.6	50.0	57.0	57.8	0.0	0.0	2.174802	46417	47049	851-856	1.000000	63.1
trnL-UAA_p5	trnL-UAA	5	GCTAGACTACACTACGTCGA	TCTCTTCGCACTCCTTTTGG	20	20	50.0	50.0	56.0	57.8	0.0	0.0	2.196754	46417	47049	849-854	1.000000	62.9
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GCCCTGAAATCCAAAATGCC	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.104399	49740	50654	1025-1025	1.000000	79.7
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GCCCTGAAATCCAAAATGCC	CGGTTCGAATCCGTATAGCC	20	20	50.0	55.0	58.0	58.3	0.0	22.8	0.333780	49740	50654	1026-1026	1.000000	77.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CTCTGGTCCTTCACTAACCG	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	57.3	57.9	0.0	0.0	0.732539	49740	50654	1054-1054	1.000000	74.7
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCTGGTCCTTCACTAACCGG	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	58.7	57.9	0.0	0.0	0.812623	49740	50654	1053-1053	1.000000	74.0
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCACTAACCGGGGCTAAAAG	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	57.2	57.9	6.3	0.0	0.849972	49740	50654	1044-1044	1.000000	73.7
rbcL-psaI_p1	rbcL-psaI	1	TTCGAGCCGGTAGATACCAT	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	58.0	57.9	1.9	26.9	0.086292	55848	56797	1043-1051	1.000000	81.1
rbcL-psaI_p2	rbcL-psaI	2	AGCCGGTAGATACCATCGAT	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	57.8	57.9	1.9	26.9	0.294923	55848	56797	1039-1047	1.000000	79.5
rbcL-psaI_p3	rbcL-psaI	3	TTCGAGCCGGTAGATACCAT	GAAGTCATTGCAATTGCCGG	20	20	50.0	50.0	58.0	58.4	1.9	26.9	0.376457	55848	56797	1046-1054	1.000000	78.8
rbcL-psaI_p4	rbcL-psaI	4	AGCTTGCAAATGGAGTCCTG	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	58.5	57.9	11.3	26.9	0.534920	55848	56797	1107-1115	1.000000	77.4
rbcL-psaI_p5	rbcL-psaI	5	CAGCTTGCAAATGGAGTCCT	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	58.5	57.9	11.3	26.9	0.534920	55848	56797	1108-1116	1.000000	77.4
psaJ-rpl33_p1	psaJ-rpl33	1	TATCTCTCTGTAGCACCCGT	GAAATCCCCGTCGATTCCTC	20	20	50.0	55.0	57.3	58.1	0.0	0.0	0.863768	63895	64326	608-635	1.000000	76.8
psaJ-rpl33_p2	psaJ-rpl33	2	TATCTCTCTGTAGCACCCGT	GGCACCTTTTCGAACACAAC	20	20	50.0	50.0	57.3	58.2	0.0	0.0	0.895813	63895	64326	585-612	1.000000	76.7
psaJ-rpl33_p3	psaJ-rpl33	3	TATCTCTCTGTAGCACCCGT	CGATTCCTCATTGGCACCTT	20	20	50.0	50.0	57.3	58.2	0.0	0.0	0.975755	63895	64326	597-624	1.000000	76.0
psaJ-rpl33_p4	psaJ-rpl33	4	ATCTCTCTGTAGCACCCGTG	GAAATCCCCGTCGATTCCTC	20	20	55.0	55.0	58.9	58.1	0.0	0.0	1.025719	63895	64326	607-634	1.000000	75.5
psaJ-rpl33_p5	psaJ-rpl33	5	ATCTCTCTGTAGCACCCGTG	GGCACCTTTTCGAACACAAC	20	20	55.0	50.0	58.9	58.2	0.0	0.0	1.057765	63895	64326	584-611	1.000000	75.4
rpl33-rps18_p1	rpl33-rps18	1	TCGCAAGCATACGACTCATC	GAAAAGGTTGTTTAGATGTATACA	20	24	50.0	29.2	58.2	53.1	0.0	8.3	9.082267	64528	64808	340-343	1.000000	41.2
rpl33-rps18_p2	rpl33-rps18	2	CGCAAGCATACGACTCATCA	GAAAAGGTTGTTTAGATGTATACA	20	24	50.0	29.2	58.4	53.1	0.0	8.3	9.362616	64528	64808	339-342	1.000000	41.2
rpl33-rps18_p3	rpl33-rps18	3	AGAATCGCCACAATACACCC	GAAAAGGTTGTTTAGATGTATACA	20	24	50.0	29.2	58.0	53.1	0.0	8.3	8.960834	64528	64808	395-398	1.000000	41.0
rpl33-rps18_p4	rpl33-rps18	4	CCACAATACACCCGGACAAT	GAAAAGGTTGTTTAGATGTATACA	20	24	50.0	29.2	57.9	53.1	0.7	8.3	9.045231	64528	64808	388-391	1.000000	41.0
rpl33-rps18_p5	rpl33-rps18	5	AGAATCGCCACAATACACCC	GAAAAGGTTGTTTAGATGTATACAT	20	25	50.0	28.0	58.0	53.6	0.0	18.3	9.388888	64528	64808	395-398	1.000000	41.0
rps18_p1	rps18	1	AGACTACTTCTTCTGGATCCGA	GGTAGGAATCAACGAGCTGG	22	20	45.5	55.0	57.8	58.1	7.4	0.0	2.296651	64809	65321	672-699	1.000000	65.9
rps18_p2	rps18	2	GACTACTTCTTCTGGATCCGA	GGTAGGAATCAACGAGCTGG	21	20	47.6	55.0	56.3	58.1	7.4	0.0	2.765598	64809	65321	671-698	1.000000	62.2
rps18_p3	rps18	3	AGACTACTTCTTCTGGATCCG	GGTAGGAATCAACGAGCTGG	21	20	47.6	55.0	56.3	58.1	7.4	0.0	2.775228	64809	65321	672-699	1.000000	62.1
rps18_p4	rps18	4	AGACTACTTCTTCTGGATCCGA	TGGTAGGAATCAACGAGCTG	22	20	45.5	50.0	57.8	57.3	7.4	0.0	2.910938	64809	65321	673-700	1.000000	61.0
rps18_p5	rps18	5	AGACTACTTCTTCTGGATCCGA	GGTCTGGCCCTTTCGAATAA	22	20	45.5	50.0	57.8	57.6	7.4	0.0	2.651286	64809	65321	630-633	1.000000	60.4
rps8-rpl14_p1	rps8-rpl14	1	TCAGCAATAGTGTCCTTGCC	CGATTGCCGAGGAATTGAGA	20	20	50.0	50.0	57.9	58.1	3.8	0.0	0.173886	76206	76348	234-234	1.000000	80.0
rps8-rpl14_p2	rps8-rpl14	2	TCAGCAATAGTGTCCTTGCC	ATCCAAAAGGAACTCGCGTT	20	20	50.0	45.0	57.9	58.1	3.8	5.8	0.221651	76206	76348	261-261	1.000000	79.5
rps8-rpl14_p3	rps8-rpl14	3	TCAGCAATAGTGTCCTTGCC	AATCCAAAAGGAACTCGCGT	20	20	50.0	45.0	57.9	58.1	3.8	5.8	0.221651	76206	76348	262-262	1.000000	79.4
rps8-rpl14_p4	rps8-rpl14	4	GCAATAGTGTCCTTGCCCAT	CGATTGCCGAGGAATTGAGA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.292267	76206	76348	231-231	1.000000	79.0
rps8-rpl14_p5	rps8-rpl14	5	GCAATAGTGTCCTTGCCCAT	ATCCAAAAGGAACTCGCGTT	20	20	50.0	45.0	58.2	58.1	0.0	5.8	0.340032	76206	76348	258-258	1.000000	78.5
rrn16_p1	rrn16	1	CCGAATCCGCTTTGTCTACA	TTTTGGGCGTGAAGAAGTGT	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.246900	91347	92839	1606-1608	1.000000	79.7
rrn16_p2	rrn16	2	CCGAATCCGCTTTGTCTACA	GCGTGAAGAAGTGTCAAACC	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.389481	91347	92839	1600-1602	1.000000	78.5
rrn16_p3	rrn16	3	CCGAATCCGCTTTGTCTACA	GGCGTGAAGAAGTGTCAAAC	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.389481	91347	92839	1601-1603	1.000000	78.5
rrn16_p4	rrn16	4	TCCGAATCCGCTTTGTCTAC	TTTTGGGCGTGAAGAAGTGT	20	20	50.0	45.0	57.7	58.2	0.0	0.0	0.531279	91347	92839	1607-1609	1.000000	77.4
rrn16_p5	rrn16	5	ATTCCGAATCCGCTTTGTCT	TTTTGGGCGTGAAGAAGTGT	20	20	45.0	45.0	57.6	58.2	0.0	0.0	0.645569	91347	92839	1609-1611	1.000000	76.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGGCAACAACAACAAACAA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.104313	104701	105331	719-725	1.000000	81.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	ACATCCAAAACCAAAGGGCT	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.105348	104701	105331	743-749	1.000000	81.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGGGCAACAACAACAAACAA	GGATTCGAACCGAGATGCTT	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.159280	104701	105331	722-728	1.000000	80.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGGGCAACAACAACAAACAA	GATCCGCACAAAAGAGTTGC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.131501	104701	105331	1271-1277	1.000000	80.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ACATCCAAAACCAAAGGGCT	GATCCGCACAAAAGAGTTGC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.132536	104701	105331	1295-1301	1.000000	80.1