primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	146	333	252-258	1.000000	85.6
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	146	333	266-272	1.000000	85.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	146	333	265-271	1.000000	85.2
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	146	333	267-273	1.000000	85.1
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACAATCCACTGCCTT	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	146	333	272-278	1.000000	85.0
psbA_p1	psbA	1	TGCTCCTTTATTTTCAAAAACTCGT	TTGTGTACTTGGGAGTCCCT	25	20	32.0	50.0	58.2	57.9	0.0	28.7	5.310704	334	1395	1153-1153	1.000000	40.2
psbA_p2	psbA	2	TGCTCCTTTATTTTCAAAAACTCG	TTGTGTACTTGGGAGTCCCT	24	20	33.3	50.0	56.7	57.9	0.0	28.7	5.418638	334	1395	1153-1153	1.000000	40.2
psbA_p3	psbA	3	GCTCCTTTATTTTCAAAAACTCGT	TTGTGTACTTGGGAGTCCCT	24	20	33.3	50.0	56.7	57.9	0.0	28.7	5.420507	334	1395	1152-1152	1.000000	40.2
psbA_p4	psbA	4	TGCTCCTTTATTTTCAAAAACTCGT	ACTTGGGAGTCCCTGATGAT	25	20	32.0	50.0	58.2	57.7	0.0	28.7	5.475433	334	1395	1147-1147	1.000000	40.2
psbA_p5	psbA	5	TGCTCCTTTATTTTCAAAAACTCGT	GTGTACTTGGGAGTCCCTGA	25	20	32.0	55.0	58.2	58.4	0.0	28.7	5.557509	334	1395	1151-1151	1.000000	40.2
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	TTTGGTCCCGGTGATCAAAG	20	20	45.0	50.0	58.1	58.1	8.1	20.7	0.235037	6136	7706	1661-1902	1.000000	81.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	CCGGTGATCAAAGGTTCGAA	20	20	45.0	50.0	58.1	58.2	8.1	0.0	0.335990	6136	7706	1654-1895	1.000000	80.6
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	AATAGACAGAATGGGGCGTG	20	20	45.0	50.0	58.1	57.7	8.1	0.0	0.471387	6136	7706	1703-1944	1.000000	79.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	GTTTTGGTCCCGGTGATCAA	20	20	45.0	50.0	58.1	58.4	8.1	12.9	0.527805	6136	7706	1663-1904	1.000000	79.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	GTCCCGGTGATCAAAGGTTC	20	20	45.0	55.0	58.1	58.5	8.1	14.4	0.689299	6136	7706	1657-1898	1.000000	77.8
trnS-GCU-trnR-UCU_p1	trnS-GCU-trnR-UCU	1	AGCAAAACGGAAAGAGAGGG	TGTCCACTAAATTGCGTCCA	20	20	50.0	45.0	58.1	57.4	0.0	0.0	0.655039	9036	10611	1755-1850	1.000000	76.7
trnS-GCU-trnR-UCU_p2	trnS-GCU-trnR-UCU	2	GCAAAACGGAAAGAGAGGGA	TGTCCACTAAATTGCGTCCA	20	20	50.0	45.0	58.1	57.4	0.0	0.0	0.660390	9036	10611	1754-1849	1.000000	76.7
trnS-GCU-trnR-UCU_p3	trnS-GCU-trnR-UCU	3	ACGGAAAGAGAGGGATTCGA	TGTCCACTAAATTGCGTCCA	20	20	50.0	45.0	58.2	57.4	0.0	0.0	0.703947	9036	10611	1749-1844	1.000000	76.3
trnS-GCU-trnR-UCU_p4	trnS-GCU-trnR-UCU	4	AGCAAAACGGAAAGAGAGGG	GTGTCCACTAAATTGCGTCC	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.996747	9036	10611	1756-1851	1.000000	74.0
trnS-GCU-trnR-UCU_p5	trnS-GCU-trnR-UCU	5	GCAAAACGGAAAGAGAGGGA	GTGTCCACTAAATTGCGTCC	20	20	50.0	50.0	58.1	57.1	0.0	0.0	1.002097	9036	10611	1755-1850	1.000000	73.9
atpF_p1	atpF	1	AGTAAGCCTCACTCACTGGT	AAAGTATCGCGAGACAACCC	20	20	50.0	50.0	58.0	58.0	0.0	32.7	0.022875	12354	12908	1660-1723	1.000000	81.3
atpF_p2	atpF	2	TACCTGTTTGCCTATCCCCA	AAAGTATCGCGAGACAACCC	20	20	50.0	50.0	58.0	58.0	0.0	32.7	0.031942	12354	12908	1873-1936	1.000000	81.1
atpF_p3	atpF	3	AGTAAGCCTCACTCACTGGT	CCCAGGCGGATGGAAAAATA	20	20	50.0	50.0	58.0	57.9	0.0	3.0	0.082803	12354	12908	1642-1705	1.000000	80.9
atpF_p4	atpF	4	CGGCTTTAATGGTTGCCATG	AAAGTATCGCGAGACAACCC	20	20	50.0	50.0	58.1	58.0	1.0	32.7	0.080741	12354	12908	1366-1429	1.000000	80.8
atpF_p5	atpF	5	TACCTGTTTGCCTATCCCCA	CCCAGGCGGATGGAAAAATA	20	20	50.0	50.0	58.0	57.9	0.0	3.0	0.091870	12354	12908	1855-1918	1.000000	80.6
petN-psbM_p1	petN-psbM	1	TGTCACTCGTAGTATGGGGA	ATTGCTACTGCGCTGTTCAT	20	20	50.0	45.0	57.2	58.3	0.0	25.3	1.070075	27985	28689	816-821	1.000000	77.0
petN-psbM_p2	petN-psbM	2	ACATTTTCCCTGTCACTCGT	ATTGCTACTGCGCTGTTCAT	20	20	45.0	45.0	57.1	58.3	0.0	25.3	1.188065	27985	28689	826-831	1.000000	76.0
petN-psbM_p3	petN-psbM	3	TGTCACTCGTAGTATGGGGA	TATTGCTACTGCGCTGTTCA	20	20	50.0	45.0	57.2	57.6	0.0	25.3	1.191281	27985	28689	817-822	1.000000	76.0
petN-psbM_p4	petN-psbM	4	TGTCACTCGTAGTATGGGGA	TTGCTACTGCGCTGTTCATT	20	20	50.0	45.0	57.2	58.5	0.0	25.3	1.290168	27985	28689	815-820	1.000000	75.3
petN-psbM_p5	petN-psbM	5	ACATTTTCCCTGTCACTCGT	TATTGCTACTGCGCTGTTCA	20	20	45.0	45.0	57.1	57.6	0.0	25.3	1.309271	27985	28689	827-832	1.000000	75.0
trnY-GUA-trnE-UUC_p1	trnY-GUA-trnE-UUC	1	CGAATTTACAGTCCGTCCCC	AAGTATGCCCCCATCGTCTA	20	20	55.0	50.0	58.4	57.9	0.0	0.0	0.448606	30366	30424	181-181	1.000000	77.3
trnY-GUA-trnE-UUC_p2	trnY-GUA-trnE-UUC	2	ACGAATTTACAGTCCGTCCC	AAGTATGCCCCCATCGTCTA	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.472283	30366	30424	182-182	1.000000	77.1
trnY-GUA-trnE-UUC_p3	trnY-GUA-trnE-UUC	3	CGAATTTACAGTCCGTCCCC	CTCTCTTTCAAGGAGGCAGC	20	20	55.0	55.0	58.4	58.3	0.0	0.0	0.614884	30366	30424	147-147	1.000000	76.2
trnY-GUA-trnE-UUC_p4	trnY-GUA-trnE-UUC	4	ATCATTTCATGGCGGATCCG	AAGTATGCCCCCATCGTCTA	20	20	50.0	50.0	58.5	57.9	30.0	0.0	0.570076	30366	30424	250-250	1.000000	76.1
trnY-GUA-trnE-UUC_p5	trnY-GUA-trnE-UUC	5	CGAATTTACAGTCCGTCCCC	TCGTCTAGTGGTTCAGGACA	20	20	55.0	50.0	58.4	57.7	0.0	0.0	0.633035	30366	30424	168-168	1.000000	75.9
psbZ-trnG-UCC_p1	psbZ-trnG-UCC	1	CTTCTCCTGATGGTTGGGTG	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	57.9	57.8	0.0	1.1	0.289634	36175	36783	763-853	1.000000	83.1
psbZ-trnG-UCC_p2	psbZ-trnG-UCC	2	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.8	57.8	0.0	1.1	0.374855	36175	36783	766-856	1.000000	82.4
psbZ-trnG-UCC_p3	psbZ-trnG-UCC	3	TTCTCCTGATGGTTGGGTGA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.3	57.8	0.0	1.1	0.428804	36175	36783	762-852	1.000000	82.0
psbZ-trnG-UCC_p4	psbZ-trnG-UCC	4	CTTCTCCTGATGGTTGGGTG	TAAATACATTGCCTGGGCGG	20	20	55.0	50.0	57.9	58.3	0.0	7.1	0.433447	36175	36783	802-892	1.000000	81.7
psbZ-trnG-UCC_p5	psbZ-trnG-UCC	5	TCCTGATGGTTGGGTGAGTA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.7	57.8	0.0	1.1	0.505514	36175	36783	759-849	1.000000	81.4
trnfM-CAU-rps14_p1	trnfM-CAU-rps14	1	AAGGTTATGAGCCTTGCGAG	TTGGGCTATCCGGGCATATA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.070129	37076	37243	279-288	1.000000	83.4
trnfM-CAU-rps14_p2	trnfM-CAU-rps14	2	TTGCGGGAATGGGATTTGAA	TTGGGCTATCCGGGCATATA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.110040	37076	37243	310-319	1.000000	82.7
trnfM-CAU-rps14_p3	trnfM-CAU-rps14	3	AAATGTTGCGGGAATGGGAT	TTGGGCTATCCGGGCATATA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.175621	37076	37243	315-324	1.000000	82.1
trnfM-CAU-rps14_p4	trnfM-CAU-rps14	4	AATGTTGCGGGAATGGGATT	TTGGGCTATCCGGGCATATA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.175621	37076	37243	314-323	1.000000	82.1
trnfM-CAU-rps14_p5	trnfM-CAU-rps14	5	ATGTTGCGGGAATGGGATTT	TTGGGCTATCCGGGCATATA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.175621	37076	37243	313-322	1.000000	82.1
rps14-psaB_p1	rps14-psaB	1	AGTCAAGGAGGGAACTTTGC	TCTACCTCGGGCAAATTTGG	20	20	50.0	50.0	57.7	57.9	0.0	19.5	0.401116	37547	37675	267-267	1.000000	78.6
rps14-psaB_p2	rps14-psaB	2	GTCAAGGAGGGAACTTTGCT	TCTACCTCGGGCAAATTTGG	20	20	50.0	50.0	57.7	57.9	0.0	19.5	0.401116	37547	37675	266-266	1.000000	78.6
rps14-psaB_p3	rps14-psaB	3	AGTCAAGGAGGGAACTTTGC	CTACCTCGGGCAAATTTGGT	20	20	50.0	50.0	57.7	58.2	0.0	19.5	0.444144	37547	37675	266-266	1.000000	78.3
rps14-psaB_p4	rps14-psaB	4	GTCAAGGAGGGAACTTTGCT	CTACCTCGGGCAAATTTGGT	20	20	50.0	50.0	57.7	58.2	0.0	19.5	0.444144	37547	37675	265-265	1.000000	78.3
rps14-psaB_p5	rps14-psaB	5	GTCAAGGAGGGAACTTTGCT	CTTGATTGCCTCTACCTCGG	20	20	50.0	55.0	57.7	57.8	0.0	0.0	0.509547	37547	37675	276-276	1.000000	77.7
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	47300	48289	867-1040	1.000000	84.6
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	47300	48289	875-1048	1.000000	84.0
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	AAGAAATAGGGAAAGCCGGC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.2	58.0	21.9	0.0	0.240656	47300	48289	923-1096	1.000000	83.7
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	47300	48289	899-1072	1.000000	83.7
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	AAGAAATAGGGAAAGCCGGC	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.2	58.1	21.9	0.0	0.305465	47300	48289	931-1104	1.000000	83.1
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CCTTTTCGAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.0	57.8	1.8	0.0	0.229964	51781	52792	1155-1216	1.000000	79.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CGGGGCTAAAACTCCAGAAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.391484	51781	52792	1144-1205	1.000000	78.1
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCGAATCGGGGCTAAAACTC	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.7	57.8	1.8	0.0	0.502034	51781	52792	1150-1211	1.000000	77.2
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CCTTTTCGAATCGGGGCTAA	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	58.0	57.5	1.8	0.0	0.573736	51781	52792	1154-1215	1.000000	76.6
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCTGGACCTTTTCGAATCGG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.6	57.8	1.8	0.0	0.577367	51781	52792	1161-1222	1.000000	76.6
petG-trnW-CCA_p1	petG-trnW-CCA	1	TTTACAATACAGGCGGGGTG	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	57.9	58.0	0.0	2.6	0.132855	67254	67402	261-261	1.000000	80.2
petG-trnW-CCA_p2	petG-trnW-CCA	2	TTTACAATACAGGCGGGGTG	AGAACGTGGGTCTCCAAAAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.153655	67254	67402	239-239	1.000000	80.1
petG-trnW-CCA_p3	petG-trnW-CCA	3	TTTACAATACAGGCGGGGTG	CGCTCTTAGTTCAGTTCGGT	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.466124	67254	67402	259-259	1.000000	77.5
petG-trnW-CCA_p4	petG-trnW-CCA	4	TACAATACAGGCGGGGTGAT	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.5	58.0	0.0	2.6	0.513337	67254	67402	259-259	1.000000	77.1
petG-trnW-CCA_p5	petG-trnW-CCA	5	TACAATACAGGCGGGGTGAT	AGAACGTGGGTCTCCAAAAC	20	20	50.0	50.0	58.5	58.0	0.0	0.0	0.534138	67254	67402	237-237	1.000000	77.1
clpP_p1	clpP	1	TGCACCAACGGAAACCATAA	TCCAAAAACCCGGATTCGTT	20	20	45.0	45.0	58.0	58.0	0.0	0.7	0.033274	71551	72972	2561-2561	1.000000	81.5
clpP_p2	clpP	2	TTGCACCAACGGAAACCATA	TCCAAAAACCCGGATTCGTT	20	20	45.0	45.0	58.0	58.0	0.0	0.7	0.033274	71551	72972	2562-2562	1.000000	81.5
clpP_p3	clpP	3	GATTGGCTGTCTTGCGTTTC	TCCAAAAACCCGGATTCGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.7	0.046831	71551	72972	2331-2331	1.000000	81.5
clpP_p4	clpP	4	TGCACCAACGGAAACCATAA	AGCAAAGGCCAGAAAAGACA	20	20	45.0	45.0	58.0	57.9	0.0	5.0	0.089117	71551	72972	2609-2609	1.000000	81.0
clpP_p5	clpP	5	TTGCACCAACGGAAACCATA	AGCAAAGGCCAGAAAAGACA	20	20	45.0	45.0	58.0	57.9	0.0	5.0	0.089117	71551	72972	2610-2610	1.000000	81.0
psbN-psbH_p1	psbN-psbH	1	GGCGTACCCGGTAAAACTTA	TCAACGCTTTGTGTAGCCAT	20	20	50.0	45.0	57.6	58.1	0.0	2.6	0.487569	75383	75480	180-184	1.000000	78.7
psbN-psbH_p2	psbN-psbH	2	TAAGGCGTACCCGGTAAAAC	TCAACGCTTTGTGTAGCCAT	20	20	50.0	45.0	57.6	58.1	0.0	2.6	0.487569	75383	75480	183-187	1.000000	78.7
psbN-psbH_p3	psbN-psbH	3	GGCGTACCCGGTAAAACTTA	CAGTGGTTTCAAAAAGGCCC	20	20	50.0	50.0	57.6	57.8	0.0	0.0	0.617387	75383	75480	238-242	1.000000	77.1
psbN-psbH_p4	psbN-psbH	4	TAAGGCGTACCCGGTAAAAC	CAGTGGTTTCAAAAAGGCCC	20	20	50.0	50.0	57.6	57.8	0.0	0.0	0.617387	75383	75480	241-245	1.000000	77.0
psbN-psbH_p5	psbN-psbH	5	GGCGTACCCGGTAAAACTTA	CCTACAGCAGTTCGTCTTGG	20	20	50.0	55.0	57.6	58.3	0.0	0.0	0.663650	75383	75480	219-223	1.000000	76.9
rpl23-trnI-CAU_p1	rpl23-trnI-CAU	1	TGATTTTTCGCCAATAACCGA	GAATGGTTAAAGCGCCCAAC	21	20	38.1	50.0	56.5	58.0	0.0	2.3	2.483257	86007	86276	359-394	1.000000	67.1
rpl23-trnI-CAU_p2	rpl23-trnI-CAU	2	TGATTTTTCGCCAATAACCGA	CCTGTTAAGCATCCATGGCT	21	20	38.1	50.0	56.5	57.9	0.0	7.0	2.537027	86007	86276	379-414	1.000000	66.5
rpl23-trnI-CAU_p3	rpl23-trnI-CAU	3	TGATTTTTCGCCAATAACCGAA	GAATGGTTAAAGCGCCCAAC	22	20	36.4	50.0	57.2	58.0	0.0	2.3	2.777331	86007	86276	359-394	1.000000	64.8
rpl23-trnI-CAU_p4	rpl23-trnI-CAU	4	TTGATTTTTCGCCAATAACCGA	GAATGGTTAAAGCGCCCAAC	22	20	36.4	50.0	57.2	58.0	0.0	2.3	2.777331	86007	86276	360-395	1.000000	64.7
rpl23-trnI-CAU_p5	rpl23-trnI-CAU	5	TGATTTTTCGCCAATAACCGAA	CCTGTTAAGCATCCATGGCT	22	20	36.4	50.0	57.2	57.9	0.0	7.0	2.831101	86007	86276	379-414	1.000000	64.2
rps15-trnN-GUU_p1	rps15-trnN-GUU	1	CGACCACAACAACCGAATTG	ACGGGGGATTCGATCTCATA	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.335258	127944	128193	494-494	0.500000	57.3
rps15-trnN-GUU_p2	rps15-trnN-GUU	2	TATGAGCTGTCCCGCAAAAA	ACGGGGGATTCGATCTCATA	20	20	45.0	50.0	57.8	57.7	0.0	0.0	0.496885	127944	128193	634-634	0.500000	56.0
rps15-trnN-GUU_p3	rps15-trnN-GUU	3	TTATGAGCTGTCCCGCAAAA	ACGGGGGATTCGATCTCATA	20	20	45.0	50.0	57.8	57.7	0.0	0.0	0.496885	127944	128193	635-635	0.500000	56.0
rps15-trnN-GUU_p4	rps15-trnN-GUU	4	CGACCACAACAACCGAATTG	AACGGGGGATTCGATCTCAT	20	20	50.0	50.0	58.0	58.6	0.0	0.0	0.606399	127944	128193	495-495	0.500000	55.1
rps15-trnN-GUU_p5	rps15-trnN-GUU	5	AACATTATGAGCTGTCCCGC	ACGGGGGATTCGATCTCATA	20	20	50.0	50.0	58.3	57.7	0.0	0.0	0.635802	127944	128193	639-639	0.500000	54.9