primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ACOS_CP_t001-psbA_p1	ACOS_CP_t001-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	84	556	540-540	1.000000	78.4
ACOS_CP_t001-psbA_p2	ACOS_CP_t001-psbA	2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.199180	84	556	554-554	1.000000	78.4
ACOS_CP_t001-psbA_p3	ACOS_CP_t001-psbA	3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	84	556	553-553	1.000000	78.4
ACOS_CP_t001-psbA_p4	ACOS_CP_t001-psbA	4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	84	556	555-555	1.000000	78.4
ACOS_CP_t001-psbA_p5	ACOS_CP_t001-psbA	5	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	84	556	543-543	1.000000	78.4
psbA_p1	psbA	1	AGAGTGTTGGTTATTGCTCCT	AGAATTCGTGTGCTTGGGAG	21	20	42.9	50.0	56.9	58.2	0.0	0.0	2.264542	557	1618	1178-1178	1.000000	61.9
psbA_p2	psbA	2	AGAGTGTTGGTTATTGCTCCT	AGAGAATTCGTGTGCTTGGG	21	20	42.9	50.0	56.9	58.2	0.0	0.0	2.264542	557	1618	1180-1180	1.000000	61.9
psbA_p3	psbA	3	AGAGTGTTGGTTATTGCTCCT	GAGAATTCGTGTGCTTGGGA	21	20	42.9	50.0	56.9	58.2	0.0	0.0	2.269645	557	1618	1179-1179	1.000000	61.8
psbA_p4	psbA	4	AGAGTGTTGGTTATTGCTCCT	AATTCGTGTGCTTGGGAGTC	21	20	42.9	50.0	56.9	58.5	0.0	0.0	2.554298	557	1618	1176-1176	1.000000	59.6
psbA_p5	psbA	5	AGAGTGTTGGTTATTGCTCCT	GAATTCGTGTGCTTGGGAGT	21	20	42.9	50.0	56.9	58.5	0.0	0.0	2.554298	557	1618	1177-1177	1.000000	59.6
matK_p1	matK	1	ACATTTCCATGATCTCATAGCCA	CCTTGTTTTGACTGTATCGCA	23	21	39.1	42.9	57.7	56.8	0.0	0.0	5.482206	2191	3708	1639-1639	1.000000	40.0
matK_p2	matK	2	ACATTTCCATGATCTCATAGCCA	ACCTTGTTTTGACTGTATCGC	23	21	39.1	42.9	57.7	56.8	0.0	0.0	5.484239	2191	3708	1640-1640	1.000000	40.0
matK_p3	matK	3	ACATTTCCATGATCTCATAGCCA	CCTTGTTTTGACTGTATCGCAC	23	22	39.1	45.5	57.7	58.2	0.0	0.0	5.504345	2191	3708	1639-1639	1.000000	40.0
matK_p4	matK	4	ACATTTCCATGATCTCATAGCCA	ACCTTGTTTTGACTGTATCGCA	23	22	39.1	40.9	57.7	58.5	0.0	0.0	5.842066	2191	3708	1640-1640	1.000000	40.0
matK_p5	matK	5	ACATTTCCATGATCTCATAGCCA	TGTTTTGACTGTATCGCACT	23	20	39.1	40.0	57.7	55.3	0.0	0.0	5.986166	2191	3708	1636-1636	1.000000	40.0
matK-ACOS_CP_t002_p1	matK-ACOS_CP_t002	1	ACTCTCGAAAAAGAAGTGGGT	CGCTGTTTTGATTGCGTCAT	21	20	42.9	45.0	57.2	58.0	0.0	0.0	1.872363	3709	4464	948-948	1.000000	65.0
matK-ACOS_CP_t002_p2	matK-ACOS_CP_t002	2	ACTCTCGAAAAAGAAGTGGGT	AACGGTAGAGTACTCGGCTT	21	20	42.9	50.0	57.2	58.2	0.0	17.1	2.008544	3709	4464	859-859	1.000000	63.9
matK-ACOS_CP_t002_p3	matK-ACOS_CP_t002	3	ACTCTCGAAAAAGAAGTGGGT	ACGGTAGAGTACTCGGCTTT	21	20	42.9	50.0	57.2	58.2	0.0	17.1	2.008544	3709	4464	858-858	1.000000	63.9
matK-ACOS_CP_t002_p4	matK-ACOS_CP_t002	4	ACTCTCGAAAAAGAAGTGGGT	GGGTTGCTAACTCAACGGTA	21	20	42.9	50.0	57.2	57.5	0.0	0.0	2.300881	3709	4464	872-872	1.000000	61.6
matK-ACOS_CP_t002_p5	matK-ACOS_CP_t002	5	ACTCTCGAAAAAGAAGTGGGT	TTCGGGTTGCTAACTCAACG	21	20	42.9	50.0	57.2	58.5	0.0	0.0	2.335765	3709	4464	875-875	1.000000	61.3
rps16_p1	rps16	1	GAATCACCCACACCCTCTTC	AAACCAATGGAACGACCGAA	20	20	55.0	45.0	57.9	58.0	0.0	6.2	0.145251	5087	6203	1268-1268	1.000000	78.8
rps16_p2	rps16	2	AATCACCCACACCCTCTTCT	AAACCAATGGAACGACCGAA	20	20	50.0	45.0	58.3	58.0	0.0	6.2	0.282464	5087	6203	1267-1267	1.000000	77.7
rps16_p3	rps16	3	ATCACCCACACCCTCTTCTT	AAACCAATGGAACGACCGAA	20	20	50.0	45.0	58.3	58.0	0.0	6.2	0.282464	5087	6203	1266-1266	1.000000	77.7
rps16_p4	rps16	4	GAATCACCCACACCCTCTTC	TGGAACGACCGAACAAATGA	20	20	55.0	45.0	57.9	57.8	0.0	0.0	0.357389	5087	6203	1261-1261	1.000000	77.1
rps16_p5	rps16	5	CCCACACCCTCTTCTTTTGT	AAACCAATGGAACGACCGAA	20	20	50.0	45.0	57.6	58.0	0.0	6.2	0.394881	5087	6203	1262-1262	1.000000	76.8
rps16-ACOS_CP_t003_p1	rps16-ACOS_CP_t003	1	AGGGTGGGTGTAAGAATCCA	GAAATTGAAATGGGGCGTGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.062499	6204	7585	1550-1551	1.000000	79.6
rps16-ACOS_CP_t003_p2	rps16-ACOS_CP_t003	2	AGGGTGGGTGTAAGAATCCA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.129848	6204	7585	1492-1493	1.000000	79.0
rps16-ACOS_CP_t003_p3	rps16-ACOS_CP_t003	3	GGGTGGGTGTAAGAATCCAC	GAAATTGAAATGGGGCGTGG	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.133029	6204	7585	1549-1550	1.000000	79.0
rps16-ACOS_CP_t003_p4	rps16-ACOS_CP_t003	4	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	20	20	45.0	50.0	58.1	58.0	8.1	0.0	0.148076	6204	7585	1512-1513	1.000000	78.9
rps16-ACOS_CP_t003_p5	rps16-ACOS_CP_t003	5	GGGTGGGTGTAAGAATCCAC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	57.9	58.1	0.0	16.9	0.200378	6204	7585	1491-1492	1.000000	78.5
ACOS_CP_t004-ACOS_CP_t005_p1	ACOS_CP_t004-ACOS_CP_t005	1	CAATCCGACGCTTTAGTCCA	AAATCAAACCGAGGGATCCC	20	20	50.0	50.0	58.0	57.6	0.0	24.6	0.441668	8932	9722	904-907	1.000000	76.5
ACOS_CP_t004-ACOS_CP_t005_p2	ACOS_CP_t004-ACOS_CP_t005	2	AATCCGACGCTTTAGTCCAC	AAATCAAACCGAGGGATCCC	20	20	50.0	50.0	58.0	57.6	0.0	24.6	0.443056	8932	9722	903-906	1.000000	76.5
ACOS_CP_t004-ACOS_CP_t005_p3	ACOS_CP_t004-ACOS_CP_t005	3	ACGGAAAGAGAGGGATTCGA	AAATCAAACCGAGGGATCCC	20	20	50.0	50.0	58.2	57.6	0.0	24.6	0.588433	8932	9722	958-961	1.000000	75.3
ACOS_CP_t004-ACOS_CP_t005_p4	ACOS_CP_t004-ACOS_CP_t005	4	AGCAATCCGACGCTTTAGTC	AAATCAAACCGAGGGATCCC	20	20	50.0	50.0	58.4	57.6	0.0	24.6	0.795508	8932	9722	906-909	1.000000	73.6
ACOS_CP_t004-ACOS_CP_t005_p5	ACOS_CP_t004-ACOS_CP_t005	5	CGACGCTTTAGTCCACTCAG	AAATCAAACCGAGGGATCCC	20	20	55.0	50.0	58.4	57.6	0.0	24.6	0.818224	8932	9722	899-902	1.000000	73.5
ACOS_CP_t005_p1	ACOS_CP_t005	1	AACACACACGTCAATGCTAT	ATTCGAATACAGGTCTATCTAAT	20	23	40.0	30.4	55.7	52.3	0.0	9.4	11.000329	9723	10553	993-994	1.000000	40.1
ACOS_CP_t005_p2	ACOS_CP_t005	2	ACACACACGTCAATGCTATA	ATTCGAATACAGGTCTATCTAAT	20	23	40.0	30.4	54.8	52.3	0.0	9.4	11.866444	9723	10553	992-993	1.000000	40.1
ACOS_CP_t005_p3	ACOS_CP_t005	3	AACACACACGTCAATGCTAT	ATTCGAATACAGGTCTATCTAATA	20	24	40.0	29.2	55.7	52.4	0.0	9.4	11.906455	9723	10553	993-994	1.000000	40.1
ACOS_CP_t005_p4	ACOS_CP_t005	4	AACACACACGTCAATGCTAT	TATTCGAATACAGGTCTATCTAAT	20	24	40.0	29.2	55.7	52.4	0.0	13.8	11.906455	9723	10553	994-995	1.000000	40.1
ACOS_CP_t005_p5	ACOS_CP_t005	5	AACACACACGTCAATGCTAT	TTCGAATACAGGTCTATCTAATAT	20	24	40.0	29.2	55.7	52.4	0.0	0.0	11.906455	9723	10553	992-993	1.000000	40.1
atpA_p1	atpA	1	ACGCAAAGATGAAGAGTGATCA	TGTTTGGGGCGATGAAAGAA	22	20	40.9	45.0	58.1	58.0	0.0	0.0	2.081371	11246	12769	1650-1650	1.000000	63.3
atpA_p2	atpA	2	ACGCAAAGATGAAGAGTGATCA	ATGTTTGGGGCGATGAAAGA	22	20	40.9	45.0	58.1	57.8	0.0	0.0	2.284041	11246	12769	1651-1651	1.000000	61.7
atpA_p3	atpA	3	ACGCAAAGATGAAGAGTGATCA	CATGTTTGGGGCGATGAAAG	22	20	40.9	50.0	58.1	57.7	0.0	0.0	2.352365	11246	12769	1652-1652	1.000000	61.2
atpA_p4	atpA	4	CGCAAAGATGAAGAGTGATCA	TGTTTGGGGCGATGAAAGAA	21	20	42.9	45.0	56.4	58.0	0.0	0.0	2.652529	11246	12769	1649-1649	1.000000	58.8
atpA_p5	atpA	5	ACGCAAAGATGAAGAGTGATC	TGTTTGGGGCGATGAAAGAA	21	20	42.9	45.0	56.4	58.0	0.0	0.0	2.654851	11246	12769	1650-1650	1.000000	58.8
atpF-atpH_p1	atpF-atpH	1	CAGTGACCCAAGGAAACGAA	GGTTGTAGCCTTAGCGCTTT	20	20	50.0	50.0	58.0	58.5	0.0	18.7	0.588181	14154	14704	634-635	1.000000	75.4
atpF-atpH_p2	atpF-atpH	2	CAGTGACCCAAGGAAACGAA	ATTGGTTGTAGCCTTAGCGC	20	20	50.0	50.0	58.0	58.6	0.0	0.0	0.658680	14154	14704	637-638	1.000000	74.9
atpF-atpH_p3	atpF-atpH	3	CAGTGACCCAAGGAAACGAA	CGGAGGGAAAAATACGAGGT	20	20	50.0	50.0	58.0	57.3	0.0	0.0	0.724056	14154	14704	704-705	1.000000	74.3
atpF-atpH_p4	atpF-atpH	4	CAGTGACCCAAGGAAACGAA	GCGGAGGGAAAAATACGAGG	20	20	50.0	55.0	58.0	58.7	0.0	0.0	0.741479	14154	14704	705-706	1.000000	74.2
atpF-atpH_p5	atpF-atpH	5	CAGTGACCCAAGGAAACGAA	GGCGGAGGGAAAAATACGAG	20	20	50.0	55.0	58.0	58.7	0.0	0.0	0.741479	14154	14704	706-707	1.000000	74.2
rpoB_p1	rpoB	1	TCTTCTGGGGCATACGATGA	ACTCATGTGACAAGCCAAGG	20	20	50.0	50.0	58.2	58.1	0.0	0.9	0.310180	26135	26254	185-185	1.000000	79.2
rpoB_p2	rpoB	2	TCTTCTGGGGCATACGATGA	TCATGTGACAAGCCAAGGAC	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.315551	26135	26254	183-183	1.000000	79.2
rpoB_p3	rpoB	3	TCTTCTGGGGCATACGATGA	CAGACTCATGTGACAAGCCA	20	20	50.0	50.0	58.2	57.8	0.0	0.9	0.386134	26135	26254	188-188	1.000000	78.6
rpoB_p4	rpoB	4	TCTTCTGGGGCATACGATGA	CTCATGTGACAAGCCAAGGA	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.401365	26135	26254	184-184	1.000000	78.5
rpoB_p5	rpoB	5	TCTTCTGGGGCATACGATGA	TCAGACTCATGTGACAAGCC	20	20	50.0	50.0	58.2	57.5	0.0	0.9	0.674227	26135	26254	189-189	1.000000	76.3
petN-psbM_p1	petN-psbM	1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTCT	20	21	45.0	42.9	58.0	57.0	0.0	0.0	1.975331	30690	31714	1126-1180	1.000000	68.0
petN-psbM_p2	petN-psbM	2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTCTTCATTCT	20	21	50.0	42.9	57.8	57.0	0.0	0.0	2.160415	30690	31714	1132-1186	1.000000	66.5
petN-psbM_p3	petN-psbM	3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTCTTCATTCT	20	21	50.0	42.9	57.6	57.0	0.0	0.0	2.320150	30690	31714	1072-1126	1.000000	65.4
petN-psbM_p4	petN-psbM	4	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTC	20	20	45.0	45.0	58.0	55.5	0.0	0.0	2.521758	30690	31714	1126-1180	1.000000	63.6
petN-psbM_p5	petN-psbM	5	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTCTTCATTC	20	20	50.0	45.0	57.8	55.5	0.0	0.0	2.706841	30690	31714	1132-1186	1.000000	62.1
rps15-ycf1_p1	rps15-ycf1	1	TTGAAATTCAACGGACCCCC	AATAATGGGAGCCGCTTCAC	20	20	50.0	50.0	58.4	58.3	4.0	0.0	0.696499	128822	129371	654-688	1.000000	78.4
rps15-ycf1_p2	rps15-ycf1	2	TTGAAATTCAACGGACCCCC	CAATAATGGGAGCCGCTTCA	20	20	50.0	50.0	58.4	58.3	4.0	0.0	0.697698	128822	129371	655-689	1.000000	78.4
rps15-ycf1_p3	rps15-ycf1	3	TTGAAATTCAACGGACCCCC	TAATGGGAGCCGCTTCACTA	20	20	50.0	50.0	58.4	58.5	4.0	0.0	0.885168	128822	129371	652-686	1.000000	76.9
rps15-ycf1_p4	rps15-ycf1	4	GGACCCCCTGTTTTCTTCTT	AATAATGGGAGCCGCTTCAC	20	20	50.0	50.0	57.3	58.3	0.0	0.0	0.997204	128822	129371	642-676	1.000000	76.0
rps15-ycf1_p5	rps15-ycf1	5	GGACCCCCTGTTTTCTTCTT	CAATAATGGGAGCCGCTTCA	20	20	50.0	50.0	57.3	58.3	0.0	0.0	0.998403	128822	129371	643-677	1.000000	76.0