primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	1122	1316	295-338	1.000000	90.7
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	1122	1316	309-352	1.000000	90.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	1122	1316	308-351	1.000000	90.4
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	1122	1316	310-353	1.000000	90.4
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	1122	1316	245-288	1.000000	90.4
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	GGGGGTTACTCGAACGAATC	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.106300	9418	9983	622-671	1.000000	88.7
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	ACGGAAAGAGAGGGATTCGA	GGGGGTTACTCGAACGAATC	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.219442	9418	9983	671-720	1.000000	87.4
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	GCTTTAGTCCACTCAGCCAT	GGGGGTTACTCGAACGAATC	20	20	50.0	55.0	57.6	58.1	0.9	0.0	0.478042	9418	9983	608-657	1.000000	85.9
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	ATTAGCAATCCGCCGCTTTA	AGGGGGTTACTCGAACGAAT	20	20	45.0	50.0	58.0	58.4	0.0	0.0	0.473140	9418	9983	623-672	1.000000	85.8
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AGTCCACTCAGCCATCTCTC	GGGGGTTACTCGAACGAATC	20	20	55.0	55.0	58.5	58.1	0.0	0.0	0.584056	9418	9983	603-652	1.000000	85.1
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	58.0	57.9	1.4	21.3	0.104185	14458	15377	799-1246	1.000000	86.5
atpH-atpI_p2	atpH-atpI	2	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	20	20	50.0	55.0	57.9	57.9	0.0	21.3	0.178077	14458	15377	855-1302	1.000000	85.8
atpH-atpI_p3	atpH-atpI	3	AGCCAATCCAGCAGCAATAA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	57.9	57.9	0.0	21.3	0.214244	14458	15377	815-1262	1.000000	85.6
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	58.2	57.9	1.4	21.3	0.249724	14458	15377	800-1247	1.000000	85.3
atpH-atpI_p5	atpH-atpI	5	GCAGTACCTTGACCAACTCC	ATAGGGGAATCCATGGAGGG	20	20	55.0	55.0	58.2	57.9	0.0	21.3	0.256114	14458	15377	859-1306	1.000000	85.1
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	GTGTAGTACCCTACCCCCAG	GAACCGATGACTTACGCCTT	20	20	60.0	50.0	58.2	58.0	0.0	0.0	0.220324	32169	32931	327-1042	1.000000	89.1
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	GTGTAGTACCCTACCCCCAG	TACCGCTGAGTTAAAAGGGC	20	20	60.0	50.0	58.2	57.9	0.0	0.0	0.317890	32169	32931	292-1007	1.000000	88.6
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	32169	32931	292-1007	0.965517	88.2
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.273369	32169	32931	257-972	0.965517	87.7
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.320736	32169	32931	289-1004	0.965517	87.0
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	20	20	50.0	45.0	57.9	58.1	4.1	11.5	0.219123	43431	44137	800-845	1.000000	85.1
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	57.9	57.8	4.1	0.0	0.279161	43431	44137	787-832	1.000000	84.7
psaA-ycf3_p3	psaA-ycf3	3	TTCTTGAGAAATGACCCGGC	AGCGCAGAATTGGTTGAAGA	20	20	50.0	45.0	58.2	58.1	0.0	11.5	0.282564	43431	44137	823-868	1.000000	84.6
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	57.9	58.2	4.1	0.0	0.306932	43431	44137	791-836	1.000000	84.5
psaA-ycf3_p5	psaA-ycf3	5	TTCTTGAGAAATGACCCGGC	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	58.2	57.8	0.0	0.0	0.342602	43431	44137	810-855	1.000000	84.1
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	58.0	58.1	8.2	0.0	1.134847	46141	46969	899-921	1.000000	79.1
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	21	20	47.6	45.0	58.0	57.8	8.2	0.0	1.233148	46141	46969	870-892	1.000000	78.6
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	46141	46969	920-942	1.000000	78.4
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	21	20	47.6	50.0	58.0	58.3	8.2	0.0	1.301418	46141	46969	872-894	1.000000	78.0
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	21	20	47.6	50.0	58.0	58.4	8.2	0.0	1.427808	46141	46969	900-922	1.000000	76.7
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	48400	49191	827-884	1.000000	87.6
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	48400	49191	859-916	1.000000	86.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ATTAAGTCCGCAGCGTCTAC	20	20	55.0	50.0	57.9	57.8	0.0	15.0	0.338047	48400	49191	845-902	1.000000	85.9
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	ATTAAGTCCGCAGCGTCTAC	20	20	50.0	50.0	57.8	57.8	15.5	15.0	0.445800	48400	49191	877-934	1.000000	84.9
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATGCGATGCTCTAACCTCTG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.5	58.0	0.0	0.0	0.527459	48400	49191	829-886	1.000000	84.5
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCAGGCCCTTTGTTAATCGG	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.230316	52291	53369	1118-1283	0.965517	84.9
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TAATCGGGGCTAAAACTCCG	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.4	58.1	0.0	0.0	0.715163	52291	53369	1105-1270	1.000000	82.5
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CCCTTTGTTAATCGGGGCTA	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.3	58.1	0.0	0.0	0.828958	52291	53369	1113-1278	1.000000	81.5
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGGGCTAAAACTCCGGAAAC	CCGAGAAAGTCTACGGTTCG	20	20	55.0	55.0	58.8	58.1	5.2	0.0	0.936224	52291	53369	1100-1265	1.000000	80.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCAGGCCCTTTGTTAATCGG	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.806132	52291	53369	1103-1268	0.965517	80.3
accD_p1	accD	1	CATGCAAAGCAAATAGGGGG	ATGTCAACAAAGAAACCCCA	20	20	50.0	40.0	57.4	55.3	0.0	0.0	3.262734	59187	60656	1566-1596	0.965517	55.7
accD_p2	accD	2	CATGCAAAGCAAATAGGGGG	TGTCAACAAAGAAACCCCAT	20	20	50.0	40.0	57.4	55.3	0.0	0.0	3.262734	59187	60656	1565-1595	0.965517	55.7
accD_p3	accD	3	CATGCAAAGCAAATAGGGGG	GTCAACAAAGAAACCCCATTCA	20	22	50.0	40.9	57.4	57.3	0.0	0.0	3.271781	59187	60656	1564-1594	0.965517	55.6
accD_p4	accD	4	CATGCAAAGCAAATAGGGGG	TGTCAACAAAGAAACCCCATTC	20	22	50.0	40.9	57.4	57.3	0.0	0.0	3.271781	59187	60656	1565-1595	0.965517	55.6
accD_p5	accD	5	AGCAAATAGGGGGCAAGAAA	TGTCAACAAAGAAACCCCAT	20	20	45.0	40.0	57.4	55.3	0.0	0.0	3.276659	59187	60656	1558-1588	0.965517	55.6
petA-psbJ_p1	petA-psbJ	1	CTTACGTGTCCAAGGCCTTT	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	58.1	58.1	17.6	0.0	1.201437	64311	65260	1116-1187	0.965517	79.3
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	57.8	58.1	0.0	0.0	1.248500	64311	65260	1103-1174	0.965517	78.9
petA-psbJ_p3	petA-psbJ	3	CTTACGTGTCCAAGGCCTTT	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	58.1	57.8	17.6	0.0	1.293037	64311	65260	1117-1188	0.965517	78.6
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	57.8	57.8	0.0	0.0	1.340100	64311	65260	1104-1175	0.965517	78.2
petA-psbJ_p5	petA-psbJ	5	GATCCCTTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	20	21	55.0	47.6	57.7	58.1	7.7	0.0	1.404770	64311	65260	1121-1192	0.965517	77.7
ycf2_p1	ycf2	1	TGGGCTCCTAGAATATGGCA	TGGTTCCACTCTGCAAGAAC	20	20	50.0	50.0	57.9	58.0	0.0	3.4	0.149551	93252	93371	160-178	1.000000	90.8
ycf2_p2	ycf2	2	TGGGCTCCTAGAATATGGCA	CACTCTGCAAGAACTCCGAA	20	20	50.0	50.0	57.9	57.8	0.0	3.4	0.271723	93252	93371	154-172	1.000000	89.8
ycf2_p3	ycf2	3	TGGGCTCCTAGAATATGGCA	GTGTCTGGTACTGCATGGTT	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.301873	93252	93371	175-193	1.000000	89.6
ycf2_p4	ycf2	4	TGGGCTCCTAGAATATGGCA	CATGGTTCCACTCTGCAAGA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.306463	93252	93371	162-180	1.000000	89.5
ycf2_p5	ycf2	5	TGGGCTCCTAGAATATGGCA	CTGCAGGGTCCCAAATGAAT	20	20	50.0	50.0	57.9	58.1	0.0	11.6	0.260240	93252	93371	238-256	1.000000	88.7
ndhF_p1	ndhF	1	CTCATGAAGGCCTACCGTTG	GGGGGTAGGATCCGGTAAAT	20	20	55.0	55.0	58.3	58.3	5.2	6.0	0.604874	111483	113729	2359-2450	0.551724	61.6
ndhF_p2	ndhF	2	AATTGGAATTCCTGTCGGGC	GGGGGTAGGATCCGGTAAAT	20	20	50.0	55.0	58.5	58.3	24.7	6.0	0.787197	111483	113729	2383-2474	0.551724	60.1
ndhF_p3	ndhF	3	ATGAAGGCCTACCGTTGGAT	GGGGGTAGGATCCGGTAAAT	20	20	50.0	55.0	58.8	58.3	5.2	6.0	1.048231	111483	113729	2356-2447	0.551724	58.0
ndhF_p4	ndhF	4	CTCATGAAGGCCTACCGTTG	GGGTAGGATCCGGTAAATCG	20	20	55.0	55.0	58.3	57.3	5.2	6.0	1.081361	111483	113729	2357-2448	0.551724	57.8
ndhF_p5	ndhF	5	CTCATGAAGGCCTACCGTTG	TTGAAAGGGGGTAGGATCCG	20	20	55.0	55.0	58.3	58.8	5.2	6.0	1.133726	111483	113729	2365-2456	0.551724	57.3
ndhF-rpl32_p1	ndhF-rpl32	1	AAGCGGGTCGATCAAGTAAC	TGACAAACAGGTTGATGGGG	20	20	50.0	50.0	58.0	58.0	0.0	4.8	0.012015	113730	114772	1702-1781	1.000000	88.3
ndhF-rpl32_p2	ndhF-rpl32	2	AAGCGGGTCGATCAAGTAAC	AATTCCAGGCTTTTGTCGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.010594	113730	114772	1951-2044	0.965517	87.2
ndhF-rpl32_p3	ndhF-rpl32	3	AAGCGGGTCGATCAAGTAAC	ATTCCAGGCTTTTGTCGGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.010594	113730	114772	1950-2043	0.965517	87.2
ndhF-rpl32_p4	ndhF-rpl32	4	CCGTGTGAACCAAAACCCTA	AATTCCAGGCTTTTGTCGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.034532	113730	114772	2179-2272	0.965517	86.7
ndhF-rpl32_p5	ndhF-rpl32	5	CCGTGTGAACCAAAACCCTA	ATTCCAGGCTTTTGTCGGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.034532	113730	114772	2178-2271	0.965517	86.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGGGATATTGGGCAGCCTTA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.174643	114935	115850	1030-1125	1.000000	88.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGATATTGGGCAGCCTTA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.176014	114935	115850	1031-1126	1.000000	88.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGGATATTGGGCAGCCTTA	CTCGCACTGCTTCCTAAGAG	20	20	50.0	55.0	58.2	58.1	12.4	0.0	0.246895	114935	115850	1051-1146	1.000000	87.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGATATTGGGCAGCCTTA	CTCTCGCACTGCTTCCTAAG	20	20	50.0	55.0	58.2	58.1	12.4	0.0	0.246895	114935	115850	1053-1148	1.000000	87.3
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGGATATTGGGCAGCCTTA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.2	57.9	12.4	0.0	0.291090	114935	115850	1040-1135	1.000000	87.1
ndhG-ndhI_p1	ndhG-ndhI	1	AAGACCCGATCCCAGAAAGA	AGGGCGTTTACCAATGTCAA	20	20	50.0	45.0	58.0	57.7	0.0	0.0	0.327313	120416	120776	489-519	1.000000	85.4
ndhG-ndhI_p2	ndhG-ndhI	2	AAGACCCGATCCCAGAAAGA	TGCTTTAGGGCGTTTACCAA	20	20	50.0	45.0	58.0	57.7	0.0	0.0	0.320917	120416	120776	495-525	1.000000	85.3
ndhG-ndhI_p3	ndhG-ndhI	3	AAGACCCGATCCCAGAAAGA	TTGCTTTAGGGCGTTTACCA	20	20	50.0	45.0	58.0	57.7	0.0	0.0	0.320917	120416	120776	496-526	1.000000	85.3
ndhG-ndhI_p4	ndhG-ndhI	4	AATACCACTCCCAGACCTCC	TGCTTTAGGGCGTTTACCAA	20	20	55.0	45.0	58.1	57.7	0.0	0.0	0.392597	120416	120776	521-551	1.000000	84.7
ndhG-ndhI_p5	ndhG-ndhI	5	AATACCACTCCCAGACCTCC	TTGCTTTAGGGCGTTTACCA	20	20	55.0	45.0	58.1	57.7	0.0	0.0	0.392597	120416	120776	522-552	1.000000	84.7
ycf1_p1	ycf1	1	TCCGCGATTTTTATCCACGT	ACATGCCGAAGTGATGGAAA	20	20	45.0	45.0	58.0	57.8	8.2	0.0	0.211764	129221	129340	1079-1181	1.000000	87.1
ycf1_p2	ycf1	2	TCCGCGATTTTTATCCACGT	GATGGAATCGCCCATTACGA	20	20	45.0	50.0	58.0	57.8	8.2	0.0	0.176755	129221	129340	1162-1264	1.000000	87.0
ycf1_p3	ycf1	3	CACTCATCGAAGTCCTCAGC	GATGGAATCGCCCATTACGA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.243900	129221	129340	1327-1429	1.000000	86.4
ycf1_p4	ycf1	4	TACGCCTTTGAACACGCATA	GATGGAATCGCCCATTACGA	20	20	45.0	50.0	57.9	57.8	0.0	0.0	0.245543	129221	129340	966-1032	1.000000	86.3
ycf1_p5	ycf1	5	TGCGCATTTGCTTGAAACAA	GATGGAATCGCCCATTACGA	20	20	40.0	50.0	58.1	57.8	5.7	0.0	0.231912	129221	129340	1003-1069	0.931034	83.8