primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	GCCGTACGAGGAGAAAACTT	20	20	50.0	50.0	58.2	57.9	20.3	18.8	0.244399	4270	5176	1220-1244	1.000000	83.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	GCCGTACGAGGAGAAAACTT	20	20	50.0	50.0	58.2	57.9	20.3	18.8	0.244399	4270	5176	1219-1243	1.000000	83.0
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	AAAAGGGTATGTTGCTGCCA	20	20	50.0	45.0	58.2	58.0	20.3	0.0	0.176662	4270	5176	1623-1647	1.000000	82.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	AAAAGGGTATGTTGCTGCCA	20	20	50.0	45.0	58.2	58.0	20.3	0.0	0.176662	4270	5176	1622-1646	1.000000	82.6
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	GGGAGAAGTCCTTCCCGTAT	AAAAGGGTATGTTGCTGCCA	20	20	55.0	45.0	58.2	58.0	3.5	0.0	0.217424	4270	5176	1983-2007	1.000000	81.9
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	8756	9474	781-800	1.000000	54.8
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	8756	9474	780-799	1.000000	54.8
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8756	9474	784-803	1.000000	53.8
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8756	9474	783-802	1.000000	53.8
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.674529	8756	9474	782-801	1.000000	53.8
psaA-ycf3_p1	psaA-ycf3	1	TGAGAAATGACCCGGTTTGG	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	58.1	58.0	0.0	18.7	0.140786	41627	42612	1057-1165	1.000000	85.9
psaA-ycf3_p2	psaA-ycf3	2	CCCATGCCTCGAAAGAAGTT	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	58.2	58.0	0.0	18.7	0.218688	41627	42612	1037-1145	1.000000	85.3
psaA-ycf3_p3	psaA-ycf3	3	TGAGAAATGACCCGGTTTGG	AGGCTATAGCGCTTACTCCT	20	20	50.0	50.0	58.1	57.7	0.0	18.7	0.391786	41627	42612	1051-1159	1.000000	83.9
psaA-ycf3_p4	psaA-ycf3	4	TGAGAAATGACCCGGTTTGG	TGGTTGAAGATCACGAGACG	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.443410	41627	42612	1007-1115	1.000000	83.6
psaA-ycf3_p5	psaA-ycf3	5	TGAGAAATGACCCGGTTTGG	TTGGAAACAGGCTATAGCGC	20	20	50.0	50.0	58.1	58.3	0.0	18.3	0.432019	41627	42612	1059-1167	1.000000	83.5
clpP_p1	clpP	1	ATAGCTGACCCTCGTAGTCC	ACGCAATGGGGGATTGATAC	20	20	55.0	50.0	58.0	58.0	0.0	1.6	0.034678	70216	72177	2800-2990	1.000000	84.7
clpP_p2	clpP	2	GGATAGCTGACCCTCGTAGT	ACGCAATGGGGGATTGATAC	20	20	55.0	50.0	58.0	58.0	0.0	1.6	0.034678	70216	72177	2802-2992	1.000000	84.7
clpP_p3	clpP	3	CTGGATAGCTGACCCTCGTA	ACGCAATGGGGGATTGATAC	20	20	55.0	50.0	58.0	58.0	0.0	1.6	0.036083	70216	72177	2804-2994	1.000000	84.7
clpP_p4	clpP	4	TGGATAGCTGACCCTCGTAG	ACGCAATGGGGGATTGATAC	20	20	55.0	50.0	58.0	58.0	0.0	1.6	0.036083	70216	72177	2803-2993	1.000000	84.7
clpP_p5	clpP	5	TCTTTGGCTCTACCCCTGAA	ACGCAATGGGGGATTGATAC	20	20	50.0	50.0	58.0	58.0	0.0	1.6	0.057606	70216	72177	2893-2959	0.750000	73.8
rpoA_p1	rpoA	1	AAAGTCTGAGACGACCCAGA	AGGGTACGAGAAAAGGGACA	20	20	50.0	50.0	58.0	58.0	7.0	0.0	0.023009	78033	79076	2232-2408	1.000000	88.1
rpoA_p2	rpoA	2	AAGTCTGAGACGACCCAGAA	AGGGTACGAGAAAAGGGACA	20	20	50.0	50.0	58.0	58.0	7.0	0.0	0.023009	78033	79076	2231-2407	1.000000	88.1
rpoA_p3	rpoA	3	AGTCTGAGACGACCCAGAAA	AGGGTACGAGAAAAGGGACA	20	20	50.0	50.0	58.0	58.0	7.0	0.0	0.023009	78033	79076	2230-2406	1.000000	88.1
rpoA_p4	rpoA	4	AGTCTGAGACGACCCAGAAA	CCGAGAACTGGTTCACGTAG	20	20	50.0	55.0	58.0	58.0	7.0	23.4	0.024660	78033	79076	2471-2647	1.000000	88.0
rpoA_p5	rpoA	5	AGTCTGAGACGACCCAGAAA	CGAGAACTGGTTCACGTAGG	20	20	50.0	55.0	58.0	58.0	7.0	19.5	0.024660	78033	79076	2470-2646	1.000000	88.0
rpoA-rps11_p1	rpoA-rps11	1	CGCGTTGCAATGCCTATTAG	AGGGTACGAGAAAAGGGACA	20	20	50.0	50.0	58.0	58.0	32.3	0.0	0.042616	79077	79186	466-579	1.000000	91.7
rpoA-rps11_p2	rpoA-rps11	2	CGCGTTGCAATGCCTATTAG	CCGAGAACTGGTTCACGTAG	20	20	50.0	55.0	58.0	58.0	32.3	23.4	0.044267	79077	79186	707-820	1.000000	90.1
rpoA-rps11_p3	rpoA-rps11	3	CGCGTTGCAATGCCTATTAG	CGAGAACTGGTTCACGTAGG	20	20	50.0	55.0	58.0	58.0	32.3	19.5	0.044267	79077	79186	706-819	1.000000	90.1
rpoA-rps11_p4	rpoA-rps11	4	TACCAAGTGCTTCTACCGGA	CGAGAACTGGTTCACGTAGG	20	20	50.0	55.0	58.1	58.0	9.5	19.5	0.093971	79077	79186	1227-1340	1.000000	88.5
rpoA-rps11_p5	rpoA-rps11	5	TACCAAGTGCTTCTACCGGA	AGGGTACGAGAAAAGGGACA	20	20	50.0	50.0	58.1	58.0	9.5	0.0	0.092320	79077	79186	987-1100	1.000000	88.1
rpl23_p1	rpl23	1	ACTGTCTACGGTTCCATTGC	ATGGAATGGAACGACGTAGG	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.691666	85953	86240	482-585	1.000000	79.2
rpl23_p2	rpl23	2	TGCTCGGGGTAGAAGTTTTG	ATGGAATGGAACGACGTAGG	20	20	50.0	50.0	57.8	57.4	0.0	0.0	0.775816	85953	86240	459-562	1.000000	78.8
rpl23_p3	rpl23	3	GCTCGGGGTAGAAGTTTTGT	ATGGAATGGAACGACGTAGG	20	20	50.0	50.0	57.8	57.4	0.0	0.0	0.777336	85953	86240	458-561	1.000000	78.8
rpl23_p4	rpl23	4	GTGCTCGGGGTAGAAGTTTT	ATGGAATGGAACGACGTAGG	20	20	50.0	50.0	57.8	57.4	0.0	0.0	0.777336	85953	86240	460-563	1.000000	78.8
rpl23_p5	rpl23	5	ACTGTCTACGGTTCCATTGC	AATGGAACGACGTAGGGATC	20	20	50.0	50.0	57.9	57.1	0.0	0.0	0.979656	85953	86240	478-581	1.000000	77.0
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	AAATCGTGATCGGGATAGCG	ACGACAACGACCGAATTGAT	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.140540	107962	108828	919-1467	0.875000	81.7
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	AAATCGTGATCGGGATAGCG	GCCCTATGGAAAATGCGGTA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.090333	107962	108828	1033-1508	0.500000	64.5
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	GTGTGGGTCTTCAGCGTATT	GCCCTATGGAAAATGCGGTA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.119103	107962	108828	991-1466	0.500000	64.2
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	TTAACGGTCAAAGTGAGCCC	GCCCTATGGAAAATGCGGTA	20	20	50.0	50.0	58.1	58.0	2.1	0.0	0.137454	107962	108828	646-1121	0.500000	64.1
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	GGTAAACGCGCTTTCACATC	GCCCTATGGAAAATGCGGTA	20	20	50.0	50.0	58.1	58.0	5.7	0.0	0.145967	107962	108828	1253-1722	0.500000	64.1
rps15-ndhF_p1	rps15-ndhF	1	TTGGGAAAACGTCAACGACT	CAAATAGGGGACGGCCTTTT	20	20	45.0	50.0	58.0	58.2	9.9	7.6	0.179675	115740	115760	757-794	1.000000	84.5
rps15-ndhF_p2	rps15-ndhF	2	TTGGGAAAACGTCAACGACT	CTGGGGCGATAAGAGGATTG	20	20	45.0	55.0	58.0	57.8	9.9	0.0	0.202881	115740	115760	286-323	1.000000	83.7
rps15-ndhF_p3	rps15-ndhF	3	TTGGGAAAACGTCAACGACT	ACTGGGGCGATAAGAGGATT	20	20	45.0	50.0	58.0	58.2	9.9	0.0	0.219189	115740	115760	287-324	1.000000	83.5
rps15-ndhF_p4	rps15-ndhF	4	TTGGGAAAACGTCAACGACT	CTTGACTGGGGCGATAAGAG	20	20	45.0	55.0	58.0	57.8	9.9	0.0	0.258218	115740	115760	291-328	1.000000	83.2
rps15-ndhF_p5	rps15-ndhF	5	TTGGGAAAACGTCAACGACT	AAAACAGGCCCGAAGAGAAG	20	20	45.0	50.0	58.0	58.1	9.9	0.0	0.129443	115740	115760	389-426	0.875000	79.1
ndhF-rpl32_p1	ndhF-rpl32	1	TCCACGCATATTGATATGTCTGT	TGATGCAGAAGTACGTTTTT	23	20	39.1	35.0	57.8	53.5	11.5	0.0	7.750006	117993	118433	500-502	1.000000	43.8
ndhF-rpl32_p2	ndhF-rpl32	2	TCCACGCATATTGATATGTCTGT	TTGATGCAGAAGTACGTTTT	23	20	39.1	35.0	57.8	53.5	11.5	0.0	7.750006	117993	118433	501-503	1.000000	43.8
ndhF-rpl32_p3	ndhF-rpl32	3	TCCACGCATATTGATATGTCTGT	TTTGATGCAGAAGTACGTTT	23	20	39.1	35.0	57.8	53.5	11.5	0.0	7.750006	117993	118433	502-504	1.000000	43.8
ndhF-rpl32_p4	ndhF-rpl32	4	TCCACGCATATTGATATGTCTGT	TTTTGATGCAGAAGTACGTT	23	20	39.1	35.0	57.8	53.5	11.5	0.0	7.750006	117993	118433	503-505	1.000000	43.8
ndhF-rpl32_p5	ndhF-rpl32	5	TCCACGCATATTGATATGTCTGT	TTTTTGATGCAGAAGTACGT	23	20	39.1	35.0	57.8	53.5	11.5	0.0	7.750006	117993	118433	504-506	1.000000	43.8
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	ACGACAACGACCGAATTGAT	AAATCGTGATCGGGATAGCG	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.140540	135381	136247	919-1467	0.875000	82.3
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	GCCCTATGGAAAATGCGGTA	AAATCGTGATCGGGATAGCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.090333	135381	136247	1033-1508	0.500000	64.5
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	GCCCTATGGAAAATGCGGTA	GTGTGGGTCTTCAGCGTATT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.119103	135381	136247	991-1466	0.500000	64.2
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	GCCCTATGGAAAATGCGGTA	TTAACGGTCAAAGTGAGCCC	20	20	50.0	50.0	58.0	58.1	0.0	2.1	0.137454	135381	136247	646-1121	0.500000	64.1
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	GCCCTATGGAAAATGCGGTA	GGTAAACGCGCTTTCACATC	20	20	50.0	50.0	58.0	58.1	0.0	5.7	0.145967	135381	136247	1253-1722	0.500000	64.1
rpl23_p1	rpl23	1	ATGGAATGGAACGACGTAGG	ACTGTCTACGGTTCCATTGC	20	20	50.0	50.0	57.4	57.9	0.0	0.0	0.691666	157969	158256	482-585	1.000000	78.3
rpl23_p2	rpl23	2	ATGGAATGGAACGACGTAGG	TGCTCGGGGTAGAAGTTTTG	20	20	50.0	50.0	57.4	57.8	0.0	0.0	0.775816	157969	158256	459-562	1.000000	77.8
rpl23_p3	rpl23	3	ATGGAATGGAACGACGTAGG	GCTCGGGGTAGAAGTTTTGT	20	20	50.0	50.0	57.4	57.8	0.0	0.0	0.777336	157969	158256	458-561	1.000000	77.8
rpl23_p4	rpl23	4	ATGGAATGGAACGACGTAGG	GTGCTCGGGGTAGAAGTTTT	20	20	50.0	50.0	57.4	57.8	0.0	0.0	0.777336	157969	158256	460-563	1.000000	77.8
rpl23_p5	rpl23	5	AATGGAACGACGTAGGGATC	ACTGTCTACGGTTCCATTGC	20	20	50.0	50.0	57.1	57.9	0.0	0.0	0.979656	157969	158256	478-581	1.000000	76.0
rps11-rpoA_p1	rps11-rpoA	1	AGGGTACGAGAAAAGGGACA	CGCGTTGCAATGCCTATTAG	20	20	50.0	50.0	58.0	58.0	0.0	32.3	0.042616	165023	165132	466-579	1.000000	90.4
rps11-rpoA_p2	rps11-rpoA	2	CCGAGAACTGGTTCACGTAG	CGCGTTGCAATGCCTATTAG	20	20	55.0	50.0	58.0	58.0	23.4	32.3	0.044267	165023	165132	707-820	1.000000	89.1
rps11-rpoA_p3	rps11-rpoA	3	CGAGAACTGGTTCACGTAGG	CGCGTTGCAATGCCTATTAG	20	20	55.0	50.0	58.0	58.0	19.5	32.3	0.044267	165023	165132	706-819	1.000000	89.1
rps11-rpoA_p4	rps11-rpoA	4	CGAGAACTGGTTCACGTAGG	TACCAAGTGCTTCTACCGGA	20	20	55.0	50.0	58.0	58.1	19.5	9.5	0.093971	165023	165132	1227-1340	1.000000	88.0
rps11-rpoA_p5	rps11-rpoA	5	AGGGTACGAGAAAAGGGACA	TACCAAGTGCTTCTACCGGA	20	20	50.0	50.0	58.0	58.1	0.0	9.5	0.092320	165023	165132	987-1100	1.000000	87.7
rpoA_p1	rpoA	1	AGGGTACGAGAAAAGGGACA	AAAGTCTGAGACGACCCAGA	20	20	50.0	50.0	58.0	58.0	0.0	7.0	0.023009	165133	166176	2232-2408	1.000000	87.8
rpoA_p2	rpoA	2	AGGGTACGAGAAAAGGGACA	AAGTCTGAGACGACCCAGAA	20	20	50.0	50.0	58.0	58.0	0.0	7.0	0.023009	165133	166176	2231-2407	1.000000	87.8
rpoA_p3	rpoA	3	AGGGTACGAGAAAAGGGACA	AGTCTGAGACGACCCAGAAA	20	20	50.0	50.0	58.0	58.0	0.0	7.0	0.023009	165133	166176	2230-2406	1.000000	87.8
rpoA_p4	rpoA	4	CGAGAACTGGTTCACGTAGG	AAGTCTGAGACGACCCAGAA	20	20	55.0	50.0	58.0	58.0	19.5	7.0	0.024660	165133	166176	2471-2647	1.000000	87.7
rpoA_p5	rpoA	5	CGAGAACTGGTTCACGTAGG	AGTCTGAGACGACCCAGAAA	20	20	55.0	50.0	58.0	58.0	19.5	7.0	0.024660	165133	166176	2470-2646	1.000000	87.7