primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnH-GUG-psbA_p1 trnH-GUG-psbA 1 CTGCCTTAATCCACTTGGCT CCTCTAGACCTAGCTGCGAT 20 20 50.0 55.0 57.9 58.1 0.0 0.0 0.230502 93 326 228-486 1.000000 90.2 trnH-GUG-psbA_p2 trnH-GUG-psbA 2 CTGCCTTAATCCACTTGGCT CTGCGATTGAAGCTCCATCT 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.183961 93 326 215-473 0.966667 89.2 trnH-GUG-psbA_p3 trnH-GUG-psbA 3 CTGCCTTAATCCACTTGGCT TTCCCTCTAGACCTAGCTGC 20 20 50.0 55.0 57.9 57.9 0.0 0.0 0.209518 93 326 231-489 0.966667 89.0 trnH-GUG-psbA_p4 trnH-GUG-psbA 4 ACTGCCTTAATCCACTTGGC CTGCGATTGAAGCTCCATCT 20 20 50.0 50.0 58.2 58.0 0.0 0.0 0.209748 93 326 216-474 0.966667 89.0 trnH-GUG-psbA_p5 trnH-GUG-psbA 5 ACTGCCTTAATCCACTTGGC TTCCCTCTAGACCTAGCTGC 20 20 50.0 55.0 58.2 57.9 0.0 0.0 0.235305 93 326 232-490 0.966667 88.8 trnK-UUU-rps16_p1 trnK-UUU-rps16 1 TCGTGGCCTTTCAAACGTTA AAAAGGGTATGTTGCTGCCA 20 20 45.0 45.0 58.0 58.0 3.3 0.0 0.057414 4148 4863 1428-1534 0.200000 55.7 trnK-UUU-rps16_p2 trnK-UUU-rps16 2 CGTGGCCTTTCAAACGTTAC CCCAATGAGCCGTTTATCGA 20 20 50.0 50.0 58.0 58.1 3.3 0.0 0.078811 4148 4863 951-1063 0.166667 54.6 trnK-UUU-rps16_p3 trnK-UUU-rps16 3 CGTGGCCTTTCAAACGTTAC TCCCAATGAGCCGTTTATCG 20 20 50.0 50.0 58.0 58.1 3.3 0.0 0.078811 4148 4863 952-1064 0.166667 54.6 trnK-UUU-rps16_p4 trnK-UUU-rps16 4 CGTGGCCTTTCAAACGTTAC AAAAGGGTATGTTGCTGCCA 20 20 50.0 45.0 58.0 58.0 3.3 0.0 0.035157 4148 4863 1427-1533 0.166667 54.2 trnK-UUU-rps16_p5 trnK-UUU-rps16 5 TGGCCTTTCAAACGTTACCA AAAAGGGTATGTTGCTGCCA 20 20 45.0 45.0 57.9 58.0 3.3 0.0 0.062475 4148 4863 1425-1531 0.166667 54.0 rps16_p1 rps16 1 ACCACGCAATCCAAACATTG AGGGGTTGAAAGAATTGCGA 20 20 45.0 45.0 57.6 57.7 0.0 0.0 0.723564 4864 5989 2380-2421 0.100000 42.8 rps16_p2 rps16 2 TACCGTTGAGTTAGCAACCC AGGGGTTGAAAGAATTGCGA 20 20 50.0 45.0 57.5 57.7 0.0 0.0 0.747122 4864 5989 2444-2485 0.100000 42.6 rps16_p3 rps16 3 CCACGCAATCCAAACATTGA AGGGGTTGAAAGAATTGCGA 20 20 45.0 45.0 57.3 57.7 0.0 0.0 1.007638 4864 5989 2379-2420 0.100000 40.5 rps16_p4 rps16 4 ACTCATGTTGGATTGGCACT AGGGGTTGAAAGAATTGCGA 20 20 45.0 45.0 57.4 57.7 0.0 0.0 0.878467 4864 5989 2066-2080 0.100000 39.9 rps16_p5 rps16 5 CGTTGAGTTAGCAACCCGAA AGGGGTTGAAAGAATTGCGA 20 20 50.0 45.0 58.5 57.7 0.0 0.0 0.782005 4864 5989 2481-2482 0.066667 38.1 rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 57.2 58.1 0.0 16.9 0.863824 5990 6996 759-1085 0.966667 83.8 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 GCACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 59.1 58.1 0.0 16.9 1.134559 5990 6996 762-1088 1.000000 82.9 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 CGTTGCTTTCTACCACATCG TTCGGAGGTTCGAATCCTTC 20 20 50.0 50.0 57.2 57.3 0.0 16.9 1.455196 5990 6996 763-1089 0.966667 79.0 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 CGTTGCTTTCTACCACATCG TGATCTCGGTATTCGGAGGT 20 20 50.0 50.0 57.2 57.6 0.0 7.0 1.147704 5990 6996 774-1087 0.900000 78.8 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 GCACGTTGCTTTCTACCACA TGATCTCGGTATTCGGAGGT 20 20 50.0 50.0 59.1 57.6 0.0 7.0 1.418439 5990 6996 777-1090 0.900000 76.7 trnS-GCU-trnG-UCC_p1 trnS-GCU-trnG-UCC 1 CTTTCGTCCACTCAGCCATC TACCACTAAACTATACCCGC 20 20 55.0 45.0 58.6 54.0 0.0 0.0 4.647333 8257 8941 568-763 1.000000 54.8 trnS-GCU-trnG-UCC_p2 trnS-GCU-trnG-UCC 2 GATTCGAACCCTCGGTACAA TACCACTAAACTATACCCGC 20 20 50.0 45.0 57.3 54.0 0.0 0.0 4.671265 8257 8941 619-814 1.000000 54.6 trnS-GCU-trnG-UCC_p3 trnS-GCU-trnG-UCC 3 CTTTCGTCCACTCAGCCATC TTACCACTAAACTATACCCGC 20 21 55.0 42.9 58.6 54.9 0.0 0.0 4.783592 8257 8941 569-764 1.000000 53.7 trnS-GCU-trnG-UCC_p4 trnS-GCU-trnG-UCC 4 GATTCGAACCCTCGGTACAA TTACCACTAAACTATACCCGC 20 21 50.0 42.9 57.3 54.9 0.0 0.0 4.807525 8257 8941 620-815 1.000000 53.5 trnS-GCU-trnG-UCC_p5 trnS-GCU-trnG-UCC 5 CGGAAAGAGAGGGATTCGAA TACCACTAAACTATACCCGC 20 20 50.0 45.0 57.0 54.0 0.0 0.0 4.972602 8257 8941 631-826 1.000000 52.2 trnG-UCC-trnR-UCU_p1 trnG-UCC-trnR-UCU 1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 20 21 50.0 47.6 58.0 56.9 0.0 0.0 2.081443 9696 9944 324-349 1.000000 75.3 trnG-UCC-trnR-UCU_p2 trnG-UCC-trnR-UCU 2 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 20 21 55.0 47.6 58.1 56.9 0.0 0.0 2.110280 9696 9944 327-352 1.000000 75.1 trnG-UCC-trnR-UCU_p3 trnG-UCC-trnR-UCU 3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 20 22 50.0 45.5 58.0 57.9 0.0 0.0 2.164771 9696 9944 330-355 0.966667 73.3 trnG-UCC-trnR-UCU_p4 trnG-UCC-trnR-UCU 4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTCTGTCCT 20 22 55.0 45.5 58.1 57.9 0.0 0.0 2.193608 9696 9944 333-358 0.966667 73.1 trnG-UCC-trnR-UCU_p5 trnG-UCC-trnR-UCU 5 CCCTAGCCTTCCAAGCTAAC AGAAGACCTCTGTCCTATCCA 20 21 55.0 47.6 57.7 56.9 0.0 0.0 2.385293 9696 9944 328-353 1.000000 72.9 rpoB-trnC-GCA_p1 rpoB-trnC-GCA 1 CGAGCCCATATCCTTGCTTT TGGCGCATAGTCAAAAAGGT 20 20 50.0 45.0 58.0 58.1 0.0 10.8 0.122841 26538 27694 1961-2000 0.200000 51.2 rpoB-trnC-GCA_p2 rpoB-trnC-GCA 2 GAAAATACCGGATCCCCACC TGGCGCATAGTCAAAAAGGT 20 20 55.0 45.0 58.0 58.1 9.5 10.8 0.118657 26538 27694 2168-2207 0.200000 50.9 rpoB-trnC-GCA_p3 rpoB-trnC-GCA 3 CGACAAGCCCTGATCAATGA GAATGAGTGGCGCATAGTCA 20 20 50.0 50.0 58.0 58.1 0.0 19.5 0.088446 26538 27694 1552-1591 0.166667 50.0 rpoB-trnC-GCA_p4 rpoB-trnC-GCA 4 CGAGCCCATATCCTTGCTTT GAATGAGTGGCGCATAGTCA 20 20 50.0 50.0 58.0 58.1 0.0 19.5 0.087154 26538 27694 1968-2007 0.166667 49.5 rpoB-trnC-GCA_p5 rpoB-trnC-GCA 5 GAAAATACCGGATCCCCACC GAATGAGTGGCGCATAGTCA 20 20 55.0 50.0 58.0 58.1 9.5 19.5 0.082970 26538 27694 2175-2214 0.166667 49.3 trnT-GGU-psbD_p1 trnT-GGU-psbD 1 GCCCTTTTAACTCAGCGGTA ACAAAAACGAAACGGTCCCT 20 20 50.0 45.0 57.9 58.2 0.0 0.0 0.349607 30761 32042 1022-1463 0.966667 87.9 trnT-GGU-psbD_p2 trnT-GGU-psbD 2 GCCCTTTTAACTCAGCGGTA GGACCAGCCTACAAAAACGA 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.216651 30761 32042 1032-1473 0.933333 87.6 trnT-GGU-psbD_p3 trnT-GGU-psbD 3 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 20 20 50.0 50.0 58.3 58.1 0.0 0.0 0.395920 30761 32042 995-1436 0.966667 87.5 trnT-GGU-psbD_p4 trnT-GGU-psbD 4 GCCCTTTTAACTCAGCGGTA AGAGCAATAAACCGGACCAG 20 20 50.0 50.0 57.9 57.6 0.0 9.5 0.506789 30761 32042 1045-1486 0.933333 85.3 trnT-GGU-psbD_p5 trnT-GGU-psbD 5 GCCCTTTTAACTCAGCGGTA CAAAAACGAAACGGTCCCTC 20 20 50.0 50.0 57.9 57.6 0.0 0.0 0.498082 30761 32042 1343-1462 0.900000 84.0 psaA-pafI_p1 psaA-pafI 1 GCCCATTCCTCGAAAGAAGT ATCACTAGACGTTTCGAATAA 20 21 50.0 33.3 57.9 52.5 4.1 0.0 6.622224 40904 41589 770-804 1.000000 52.0 psaA-pafI_p2 psaA-pafI 2 TTTTACTTCTGGTTCCGGCG ATCACTAGACGTTTCGAATAA 20 21 50.0 33.3 58.5 52.5 0.0 0.0 6.991800 40904 41589 723-757 1.000000 52.0 psaA-pafI_p3 psaA-pafI 3 GGCCCATTCCTCGAAAGAAG ATCACTAGACGTTTCGAATAA 20 21 55.0 33.3 58.6 52.5 0.0 0.0 7.123358 40904 41589 771-805 1.000000 52.0 psaA-pafI_p4 psaA-pafI 4 TTTACTTCTGGTTCCGGCG ATCACTAGACGTTTCGAATAA 19 21 52.6 33.3 57.8 52.5 0.0 0.0 7.727329 40904 41589 722-756 1.000000 52.0 psaA-pafI_p5 psaA-pafI 5 CTGGCCCATTCCTCGAAAG ATCACTAGACGTTTCGAATAA 19 21 57.9 33.3 58.2 52.5 0.0 0.0 7.721013 40904 41589 773-807 0.966667 50.7 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.137364 45804 46561 771-886 1.000000 90.9 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 20 20 55.0 50.0 57.9 58.1 0.0 0.0 0.202173 45804 46561 779-894 1.000000 90.4 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 20 20 55.0 50.0 57.9 58.2 0.0 10.2 0.372548 45804 46561 785-900 1.000000 89.0 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 20 20 50.0 50.0 57.8 58.0 15.5 0.0 0.245117 45804 46561 803-918 0.966667 88.7 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 20 20 50.0 50.0 57.8 58.1 15.5 0.0 0.309926 45804 46561 811-926 0.966667 88.2 trnL-UAA-trnF-GAA_p1 trnL-UAA-trnF-GAA 1 AATCGTGAGGGTTCAAGTCC TCTACCAGCTGAGCTATCCC 20 20 50.0 55.0 57.5 58.0 0.0 17.4 0.476915 47149 47540 434-478 1.000000 88.2 trnL-UAA-trnF-GAA_p2 trnL-UAA-trnF-GAA 2 AATCGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 20 20 50.0 50.0 57.5 58.1 0.0 20.1 0.526305 47149 47540 470-514 1.000000 87.8 trnL-UAA-trnF-GAA_p3 trnL-UAA-trnF-GAA 3 AATCGTGAGGGTTCAAGTCC GTGCCAGGAACCAGATTTGA 20 20 50.0 50.0 57.5 58.1 0.0 0.0 0.564710 47149 47540 485-529 1.000000 87.5 trnL-UAA-trnF-GAA_p4 trnL-UAA-trnF-GAA 4 AATCGTGAGGGTTCAAGTCC TCAGTCCTCTGCTCTACCAG 20 20 50.0 55.0 57.5 57.9 0.0 0.0 0.610708 47149 47540 446-490 1.000000 87.1 trnL-UAA-trnF-GAA_p5 trnL-UAA-trnF-GAA 5 AATCGTGAGGGTTCAAGTCC TGTGCCAGGAACCAGATTTG 20 20 50.0 50.0 57.5 58.4 0.0 0.0 0.854756 47149 47540 486-530 1.000000 85.2 trnF-GAA-ndhJ_p1 trnF-GAA-ndhJ 1 GGGATAGCTCAGCTGGTAGA AAGGATTACATTGCCCCCAA 20 20 55.0 45.0 58.0 57.1 10.3 0.0 0.940933 47614 48271 621-795 0.966667 83.1 trnF-GAA-ndhJ_p2 trnF-GAA-ndhJ 2 CCTCGTGTCACCAGTTCAAA AAGGATTACATTGCCCCCAA 20 20 50.0 45.0 58.1 57.1 0.0 0.0 0.990323 47614 48271 585-759 0.966667 82.7 trnF-GAA-ndhJ_p3 trnF-GAA-ndhJ 3 CTGGTAGAGCAGAGGACTGA AAGGATTACATTGCCCCCAA 20 20 55.0 45.0 57.9 57.1 2.5 0.0 1.074726 47614 48271 609-783 0.966667 82.1 trnF-GAA-ndhJ_p4 trnF-GAA-ndhJ 4 GGGATAGCTCAGCTGGTAGA AGGATTACATTGCCCCCAAT 20 20 55.0 45.0 58.0 56.8 10.3 0.0 1.177056 47614 48271 620-794 0.966667 81.3 trnF-GAA-ndhJ_p5 trnF-GAA-ndhJ 5 CCTCGTGTCACCAGTTCAAA AGGATTACATTGCCCCCAAT 20 20 50.0 45.0 58.1 56.8 0.0 0.0 1.226446 47614 48271 584-758 0.966667 80.9 ndhC-trnV-UAC_p1 ndhC-trnV-UAC 1 ACATCATATTCATAAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 25 20 28.0 55.0 55.4 58.7 0.0 0.0 8.311726 49948 51040 877-1181 1.000000 52.0 ndhC-trnV-UAC_p2 ndhC-trnV-UAC 2 ACATCATATTCATAAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 25 20 28.0 50.0 55.4 57.8 0.0 0.0 7.825660 49948 51040 889-1193 0.966667 50.7 ndhC-trnV-UAC_p3 ndhC-trnV-UAC 3 ACATCATATTCATAAAGCAGAAACA GAAGGTCTACGGTTCGAGTC 25 20 28.0 55.0 55.4 57.5 0.0 0.0 8.169432 49948 51040 888-1192 0.966667 50.7 ndhC-trnV-UAC_p4 ndhC-trnV-UAC 4 AACATCATATTCATAAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 26 20 26.9 50.0 56.0 57.8 0.0 0.0 8.171460 49948 51040 890-1194 0.966667 50.7 ndhC-trnV-UAC_p5 ndhC-trnV-UAC 5 ACATCATATTCATAAAGCAGAAACAT AGAAGGTCTACGGTTCGAGT 26 20 26.9 50.0 55.8 57.8 0.0 0.0 8.353173 49948 51040 889-1193 0.966667 50.7 psbE-petL_p1 psbE-petL 1 AAAGGATCAAAACGGCCAGT AGCGCTCTTAGTTCAGTTCG 20 20 45.0 50.0 58.0 58.0 2.2 2.6 0.020651 61652 62926 1546-2084 0.966667 90.5 psbE-petL_p2 psbE-petL 2 AAGGATCAAAACGGCCAGTT AGCGCTCTTAGTTCAGTTCG 20 20 45.0 50.0 58.0 58.0 2.2 2.6 0.020651 61652 62926 1545-2083 0.966667 90.5 psbE-petL_p3 psbE-petL 3 AAAGGATCAAAACGGCCAGT TTGGCAATTGGCTGGTAAGT 20 20 45.0 45.0 58.0 58.0 2.2 29.5 0.012866 61652 62926 1147-1687 0.833333 85.2 psbE-petL_p4 psbE-petL 4 AAGGATCAAAACGGCCAGTT TTGGCAATTGGCTGGTAAGT 20 20 45.0 45.0 58.0 58.0 2.2 29.5 0.012866 61652 62926 1146-1686 0.833333 85.2 psbE-petL_p5 psbE-petL 5 GTCCGTGAATAGCCAACCAT TTGGCAATTGGCTGGTAAGT 20 20 50.0 45.0 58.0 58.0 0.0 29.5 0.046629 61652 62926 1248-1788 0.800000 83.6 ndhF-rpl32_p1 ndhF-rpl32 1 GAAGCCTTTTTGTCGCGTTT TTTTGACTGCCCAGTGGAAA 20 20 45.0 45.0 58.2 57.8 5.8 12.0 0.316381 109409 110093 758-926 0.133333 49.9 ndhF-rpl32_p2 ndhF-rpl32 2 GAAGCCTTTTTGTCGCGTTT TTTTTGACTGCCCAGTGGAA 20 20 45.0 45.0 58.2 57.8 5.8 12.0 0.316381 109409 110093 759-927 0.133333 49.8 ndhF-rpl32_p3 ndhF-rpl32 3 AAAAGCCCACATACGACGAA TTTTGACTGCCCAGTGGAAA 20 20 45.0 45.0 57.8 57.8 0.0 12.0 0.324353 109409 110093 775-943 0.133333 49.7 ndhF-rpl32_p4 ndhF-rpl32 4 AAAAGCCCACATACGACGAA TTTTTGACTGCCCAGTGGAA 20 20 45.0 45.0 57.8 57.8 0.0 12.0 0.324353 109409 110093 776-944 0.133333 49.7 ndhF-rpl32_p5 ndhF-rpl32 5 CCTTTTTGTCGCGTTTGGAA TTTTGACTGCCCAGTGGAAA 20 20 45.0 45.0 57.8 57.8 5.8 12.0 0.347797 109409 110093 754-922 0.133333 49.6 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 TTCCACTGGGCAGTCAAAAA TGCCCCTTTTTGACGAATCA 20 20 45.0 45.0 57.8 58.0 0.0 0.0 0.163209 110256 110980 662-1108 0.200000 58.7 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 TTTCCACTGGGCAGTCAAAA TGCCCCTTTTTGACGAATCA 20 20 45.0 45.0 57.8 58.0 0.0 0.0 0.163209 110256 110980 663-1109 0.200000 58.7 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 TTCCACTGGGCAGTCAAAAA AAGAACACTTGGCGCAGTTA 20 20 45.0 45.0 57.8 58.0 0.0 10.8 0.189709 110256 110980 850-1296 0.200000 58.5 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 TTCCACTGGGCAGTCAAAAA TCATGCCCCTTTTTGACGAA 20 20 45.0 45.0 57.8 58.0 0.0 0.0 0.163209 110256 110980 1085-1111 0.133333 50.0 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 TTTCCACTGGGCAGTCAAAA TCATGCCCCTTTTTGACGAA 20 20 45.0 45.0 57.8 58.0 0.0 0.0 0.163209 110256 110980 1086-1112 0.133333 50.0 ycf1_p1 ycf1 1 GCAATCGCGTTTTCTTGGTT ATCACAGCCCCAAGTTCTTG 20 20 45.0 50.0 58.2 58.1 5.8 5.7 0.318790 121978 122097 1128-1134 0.200000 49.3 ycf1_p2 ycf1 2 CACCGTCCCATCTCAATTGA ATCACAGCCCCAAGTTCTTG 20 20 50.0 50.0 57.6 58.1 8.1 5.7 0.486442 121978 122097 1253-1259 0.200000 47.8 ycf1_p3 ycf1 3 ATTCAAAGCACCGTCCCATC ATCACAGCCCCAAGTTCTTG 20 20 50.0 50.0 58.5 58.1 0.0 5.7 0.621185 121978 122097 1261-1267 0.200000 46.7 ycf1_p4 ycf1 4 GATTCAAAGCACCGTCCCAT ATCACAGCCCCAAGTTCTTG 20 20 50.0 50.0 58.5 58.1 0.0 5.7 0.621185 121978 122097 1262-1268 0.200000 46.7 ycf1_p5 ycf1 5 TGCTTCCGTATTGCTCTTGT ATCACAGCCCCAAGTTCTTG 20 20 45.0 50.0 57.5 58.1 0.0 5.7 0.557555 121978 122097 1198-1204 0.166667 45.1