primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnH-GUG-psbA_p1 trnH-GUG-psbA 1 CCACTTGGCTACATCCGC AGCTGCTGTTGAAGTTCCAT 18 20 61.1 45.0 58.2 57.7 0.0 0.0 2.469549 70 744 358-952 0.909091 68.6 trnH-GUG-psbA_p2 trnH-GUG-psbA 2 CCACTTGGCTACATCCGC GCTGCTGTTGAAGTTCCATC 18 20 61.1 50.0 58.2 57.4 0.0 0.0 2.812492 70 744 357-951 0.909091 65.9 trnH-GUG-psbA_p3 trnH-GUG-psbA 3 CCACTTGGCTACATCCGC TAGCTGCTGTTGAAGTTCCA 18 20 61.1 45.0 58.2 57.1 0.0 13.4 3.115205 70 744 359-953 0.909091 63.4 trnH-GUG-psbA_p4 trnH-GUG-psbA 4 CCACTTGGCTACATCCGC TTAGCTGCTGTTGAAGTTCCA 18 21 61.1 42.9 58.2 57.8 0.0 13.4 3.393669 70 744 360-954 0.909091 61.2 trnH-GUG-psbA_p5 trnH-GUG-psbA 5 CCACTTGGCTACATCCGC TAGCTGCTGTTGAAGTTCCAT 18 21 61.1 42.9 58.2 57.6 0.0 13.4 3.609943 70 744 359-953 0.909091 59.5 rps16_p1 rps16 1 TATGGGTGGGGATAAACGGA ATCAATTCCATCCCGGTTCG 20 20 50.0 50.0 57.8 58.0 0.0 0.0 0.226248 5513 7224 2018-2395 0.500000 70.2 rps16_p2 rps16 2 TATGGGTGGGGATAAACGGA GTTAATGTAGCGACTCGGGG 20 20 50.0 55.0 57.8 58.2 0.0 0.0 0.344470 5513 7224 2624-2984 0.454545 67.0 rps16_p3 rps16 3 AAAGCAAGAAAAGGTGCGTG ATCAATTCCATCCCGGTTCG 20 20 45.0 50.0 57.8 58.0 0.0 0.0 0.241007 5513 7224 1755-2132 0.227273 53.5 rps16_p4 rps16 4 CAAAGCAAGAAAAGGTGCGT ATCAATTCCATCCCGGTTCG 20 20 45.0 50.0 57.8 58.0 0.0 0.0 0.241007 5513 7224 1756-2133 0.227273 53.5 rps16_p5 rps16 5 AAAGCAAGAAAAGGTGCGTG GTTAATGTAGCGACTCGGGG 20 20 45.0 55.0 57.8 58.2 0.0 0.0 0.359229 5513 7224 2371-2753 0.227273 52.1 trnS-GCU-trnG-UCC_p1 trnS-GCU-trnG-UCC 1 GAGAAAGCGGAGAAAGAGGG AGGACGAATCACACTTTTACCA 20 22 55.0 40.9 58.0 57.7 0.0 0.0 2.362997 9621 11528 1040-2954 0.954545 71.3 trnS-GCU-trnG-UCC_p2 trnS-GCU-trnG-UCC 2 ATTAGCAATCCGCCGCTTTA AGGACGAATCACACTTTTACCA 20 22 45.0 40.9 58.0 57.7 0.0 0.0 2.384065 9621 11528 985-2899 0.954545 71.1 trnS-GCU-trnG-UCC_p3 trnS-GCU-trnG-UCC 3 GAGAAAGCGGAGAAAGAGGG GGACGAATCACACTTTTACCAC 20 22 55.0 45.5 58.0 57.6 0.0 0.0 2.417174 9621 11528 1039-2953 0.954545 70.8 trnS-GCU-trnG-UCC_p4 trnS-GCU-trnG-UCC 4 ATTAGCAATCCGCCGCTTTA GGACGAATCACACTTTTACCAC 20 22 45.0 45.5 58.0 57.6 0.0 0.0 2.438243 9621 11528 984-2898 0.954545 70.7 trnS-GCU-trnG-UCC_p5 trnS-GCU-trnG-UCC 5 TCAAGTGACCAGAGAAAGCG AGGACGAATCACACTTTTACCA 20 22 50.0 40.9 57.8 57.7 0.0 0.0 2.500713 9621 11528 1051-2965 0.909091 68.4 rpoB-trnC-GCA_p1 rpoB-trnC-GCA 1 CCCTCATTTTCATCCCGGAG GCGACACCCAGATTTGAACT 20 20 55.0 50.0 58.0 58.5 0.7 0.0 0.503370 29731 31263 1555-1987 0.954545 86.2 rpoB-trnC-GCA_p2 rpoB-trnC-GCA 2 CTCATTTTCATCCCGGAGCA GCGACACCCAGATTTGAACT 20 20 50.0 50.0 58.0 58.5 0.7 0.0 0.518928 29731 31263 1553-1985 0.954545 86.0 rpoB-trnC-GCA_p3 rpoB-trnC-GCA 3 CCCTCATTTTCATCCCGGAG CCTTACCACTTGGCCATGTC 20 20 55.0 55.0 58.0 58.5 0.7 20.8 0.563454 29731 31263 1509-1941 0.954545 85.7 rpoB-trnC-GCA_p4 rpoB-trnC-GCA 4 CCCTCATTTTCATCCCGGAG TAAAGGATTTGCAGTCCCCC 20 20 55.0 50.0 58.0 57.5 0.7 14.6 0.552233 29731 31263 1530-1840 0.909091 83.9 rpoB-trnC-GCA_p5 rpoB-trnC-GCA 5 CTCATTTTCATCCCGGAGCA TAAAGGATTTGCAGTCCCCC 20 20 50.0 50.0 58.0 57.5 0.7 14.6 0.567791 29731 31263 1528-1838 0.909091 83.8 trnE-UUC-trnT-GGU_p1 trnE-UUC-trnT-GGU 1 AGAGATGTCCTGAACCGCTA TCTACCACTGAGTTAAAAGGGC 20 22 50.0 45.5 57.9 57.7 0.0 0.0 2.427295 34987 35893 270-1608 0.954545 70.8 trnE-UUC-trnT-GGU_p2 trnE-UUC-trnT-GGU 2 AGATGTCCTGAACCGCTAGA TCTACCACTGAGTTAAAAGGGC 20 22 50.0 45.5 57.9 57.7 0.0 0.0 2.427295 34987 35893 268-1606 0.954545 70.8 trnE-UUC-trnT-GGU_p3 trnE-UUC-trnT-GGU 3 GATGTCCTGAACCGCTAGAC TCTACCACTGAGTTAAAAGGGC 20 22 55.0 45.5 57.8 57.7 0.0 0.0 2.493251 34987 35893 267-1605 0.954545 70.2 trnE-UUC-trnT-GGU_p4 trnE-UUC-trnT-GGU 4 GCTGCCTCCTTGAAAGAGAG TCTACCACTGAGTTAAAAGGGC 20 22 55.0 45.5 58.3 57.7 0.0 0.0 2.545287 34987 35893 286-1624 0.954545 69.8 trnE-UUC-trnT-GGU_p5 trnE-UUC-trnT-GGU 5 GAGATGTCCTGAACCGCTAG TCTACCACTGAGTTAAAAGGGC 20 22 55.0 45.5 57.5 57.7 0.0 0.0 2.782407 34987 35893 269-1607 0.954545 67.9 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.137364 51826 53579 768-1906 0.954545 89.1 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 AAGTTACCGCGGCATAACAT ACCAATTTCGCCATATCCCC 20 20 45.0 50.0 58.0 58.0 29.5 0.0 0.037304 51826 53579 1176-2090 0.863636 86.2 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 AAGTTACCGCGGCATAACAT TCCATACCAAGGCTCAATGC 20 20 45.0 50.0 58.0 57.9 29.5 0.0 0.089818 51826 53579 1215-2129 0.863636 85.8 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 AAGTTACCGCGGCATAACAT ACCAAGGCTCAATGCAATCA 20 20 45.0 45.0 58.0 58.1 29.5 0.0 0.105193 51826 53579 1210-2124 0.863636 85.7 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 AAGTTACCGCGGCATAACAT TAGCGTCTACCAATTTCGCC 20 20 45.0 50.0 58.0 58.1 29.5 0.0 0.102113 51826 53579 1184-2098 0.727273 80.3 accD_p1 accD 1 AAGCCAAGGGAGGGTATCTT TCCCATACATCGCAGCTAGA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.017544 64855 66084 2533-2869 0.590909 75.5 accD_p2 accD 2 GTCGCGTTCCTATGGAAACT CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 58.0 58.0 5.8 26.9 0.032974 64855 66084 2394-2979 0.590909 75.4 accD_p3 accD 3 AAGCCAAGGGAGGGTATCTT CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 58.0 58.0 0.0 26.9 0.039924 64855 66084 2477-2813 0.590909 75.3 accD_p4 accD 4 CATAGCTCAAGCCAAGGGAG TCCCATACATCGCAGCTAGA 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.040071 64855 66084 2541-2877 0.590909 75.3 accD_p5 accD 5 GTCGCGTTCCTATGGAAACT TCCCATACATCGCAGCTAGA 20 20 50.0 50.0 58.0 58.0 5.8 0.0 0.010594 64855 66084 2450-2815 0.545455 73.7 rpl16_p1 rpl16 1 CCGTTTTCACGCTTGAGTTC CGACGGAAAAGAAGTTGCAC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.037434 89229 91407 1946-2989 0.409091 68.1 rpl16_p2 rpl16 2 CCGTTTTCACGCTTGAGTTC GACGGAAAAGAAGTTGCACG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.037434 89229 91407 1945-2988 0.409091 68.1 rpl16_p3 rpl16 3 AATTTTCGAGCCCCGCTATT GACGGAAAAGAAGTTGCACG 20 20 45.0 50.0 57.9 58.0 0.0 0.0 0.071499 89229 91407 1780-2862 0.363636 65.2 rpl16_p4 rpl16 4 TCTCAATTCCCTGGCGATTG CGACGGAAAAGAAGTTGCAC 20 20 50.0 50.0 58.0 58.0 11.7 0.0 0.061403 89229 91407 2046-2946 0.272727 58.6 rpl16_p5 rpl16 5 TCTCAATTCCCTGGCGATTG GACGGAAAAGAAGTTGCACG 20 20 50.0 50.0 58.0 58.0 11.7 0.0 0.061403 89229 91407 2045-2945 0.272727 58.6 trnL-CAA-ndhB_p1 trnL-CAA-ndhB 1 GTCTCGCGTGTCTACCATTT TGCAATTGCTCAGGATACCC 20 20 50.0 50.0 58.0 57.9 5.8 29.4 0.064968 103560 104701 464-2578 0.909091 87.8 trnL-CAA-ndhB_p2 trnL-CAA-ndhB 2 CTCGCGTGTCTACCATTTCA TGCAATTGCTCAGGATACCC 20 20 50.0 50.0 58.0 57.9 5.8 29.4 0.066338 103560 104701 462-2576 0.909091 87.8 trnL-CAA-ndhB_p3 trnL-CAA-ndhB 3 GCGTGTCTACCATTTCACCA TGCAATTGCTCAGGATACCC 20 20 50.0 50.0 58.2 57.9 0.0 29.4 0.253803 103560 104701 459-2573 0.909091 86.3 trnL-CAA-ndhB_p4 trnL-CAA-ndhB 4 TCTCGCGTGTCTACCATTTC TGCAATTGCTCAGGATACCC 20 20 50.0 50.0 57.7 57.9 5.8 29.4 0.330507 103560 104701 463-2577 0.909091 85.7 trnL-CAA-ndhB_p5 trnL-CAA-ndhB 5 TCGCGTGTCTACCATTTCAC TGCAATTGCTCAGGATACCC 20 20 50.0 50.0 58.3 57.9 5.8 29.4 0.351881 103560 104701 461-2575 0.909091 85.5 rps7_p1 rps7 1 CCGATCGAGATGTATGGATCC TCTTGAACCTCTTTCACGCT 21 20 52.4 45.0 57.6 57.1 18.7 0.0 2.339433 107241 107708 546-552 0.954545 71.5 rps7_p2 rps7 2 CCGATCGAGATGTATGGATCC TTCTTGAACCTCTTTCACGCT 21 21 52.4 42.9 57.6 57.8 18.7 0.0 2.622751 107241 107708 547-553 0.954545 69.2 rps7_p3 rps7 3 CCGATCGAGATGTATGGATCC CTTGAACCTCTTTCACGCTC 21 20 52.4 50.0 57.6 56.8 18.7 0.0 2.668721 107241 107708 545-551 0.954545 68.8 rps7_p4 rps7 4 CCGATCGAGATGTATGGATCC TCTTGAACCTCTTTCACGCTC 21 21 52.4 47.6 57.6 58.2 18.7 0.0 2.673096 107241 107708 546-552 0.954545 68.8 rps7_p5 rps7 5 TCGATTCTTTTCCGATCGAGA TCTTGAACCTCTTTCACGCT 21 20 42.9 45.0 57.0 57.1 18.7 0.0 2.960374 107241 107708 557-563 0.863636 62.9 rps12_p1 rps12 1 TGACATGAGCGTGAAAGAGG ATTGAACGAGGAGCCGTATG 20 20 50.0 50.0 57.9 57.8 0.0 0.0 0.309678 107763 108563 940-955 0.954545 87.7 rps12_p2 rps12 2 GACATGAGCGTGAAAGAGGT ATTGAACGAGGAGCCGTATG 20 20 50.0 50.0 57.9 57.8 0.0 0.0 0.311123 107763 108563 939-954 0.954545 87.7 rps12_p3 rps12 3 CAGTACCTCGACGTGACATG ATTGAACGAGGAGCCGTATG 20 20 55.0 50.0 58.1 57.8 0.0 0.0 0.328723 107763 108563 953-968 0.954545 87.6 rps12_p4 rps12 4 TGACATGAGCGTGAAAGAGG AAGAATTGAACGAGGAGCCG 20 20 50.0 50.0 57.9 58.3 0.0 0.0 0.357756 107763 108563 944-959 0.954545 87.3 rps12_p5 rps12 5 GACATGAGCGTGAAAGAGGT AAGAATTGAACGAGGAGCCG 20 20 50.0 50.0 57.9 58.3 0.0 0.0 0.359201 107763 108563 943-958 0.954545 87.3 ycf1-ndhF_p1 ycf1-ndhF 1 TCGAAGAAGAGAGATGCGTC TATTTAGTAGGGGGAGGGCG 20 20 50.0 55.0 57.2 58.0 0.0 0.0 0.854382 125068 126802 376-2863 0.590909 68.8 ycf1-ndhF_p2 ycf1-ndhF 2 CGAAGAAGAGAGATGCGTCT TATTTAGTAGGGGGAGGGCG 20 20 50.0 55.0 57.2 58.0 8.6 0.0 0.862015 125068 126802 375-2862 0.590909 68.7 ycf1-ndhF_p3 ycf1-ndhF 3 TCGAAGAAGAGAGATGCGTC TGGTTGGCTCCATCGAAAAA 20 20 50.0 45.0 57.2 58.0 0.0 0.0 0.846281 125068 126802 775-2879 0.545455 67.0 ycf1-ndhF_p4 ycf1-ndhF 4 CGAAGAAGAGAGATGCGTCT TGGTTGGCTCCATCGAAAAA 20 20 50.0 45.0 57.2 58.0 8.6 0.0 0.853915 125068 126802 774-2878 0.545455 67.0 ycf1-ndhF_p5 ycf1-ndhF 5 TCGAAGAAGAGAGATGCGTC TGCTTTTGGTCAAGGGGAAA 20 20 50.0 45.0 57.2 57.8 0.0 0.0 1.001481 125068 126802 818-2922 0.545455 65.8 ndhG-ndhI_p1 ndhG-ndhI 1 AAACTACTCCTAGGCCTCCT TCATTCAAGGACAGTTACAA 20 20 50.0 35.0 56.7 52.0 9.7 0.0 7.232354 136757 137625 292-1529 0.954545 50.2 ndhG-ndhI_p2 ndhG-ndhI 2 AAACTACTCCTAGGCCTCCT TCATTCAAGGACAGTTACAAA 20 21 50.0 33.3 56.7 53.0 9.7 0.0 7.265052 136757 137625 292-1529 0.954545 50.2 ndhG-ndhI_p3 ndhG-ndhI 3 AAAACTACTCCTAGGCCTCCT TCATTCAAGGACAGTTACAA 21 20 47.6 35.0 57.5 52.0 9.7 0.0 7.472917 136757 137625 293-1530 0.954545 50.2 ndhG-ndhI_p4 ndhG-ndhI 4 AAAACTACTCCTAGGCCTCCT TCATTCAAGGACAGTTACAAA 21 21 47.6 33.3 57.5 53.0 9.7 0.0 7.505614 136757 137625 293-1530 0.954545 50.2 ndhG-ndhI_p5 ndhG-ndhI 5 AAACTACTCCTAGGCCTCCT TCATTCAAGGACAGTTACAAAT 20 22 50.0 31.8 56.7 53.6 9.7 0.0 7.607690 136757 137625 292-1529 0.954545 50.2 rps15-ycf1_p1 rps15-ycf1 1 TCCCAACGCGTTACATAAGG TCGAAGAAGAGAGATGCGTC 20 20 50.0 50.0 58.0 57.2 26.1 0.0 0.847511 142038 143752 887-2978 0.636364 70.7 rps15-ycf1_p2 rps15-ycf1 2 TCCCAACGCGTTACATAAGG CGAAGAAGAGAGATGCGTCT 20 20 50.0 50.0 58.0 57.2 26.1 8.6 0.855145 142038 143752 886-2977 0.636364 70.6 rps15-ycf1_p3 rps15-ycf1 3 GCTAGATAATCCCAACGCGT TCGAAGAAGAGAGATGCGTC 20 20 50.0 50.0 57.8 57.2 5.6 0.0 0.984972 142038 143752 896-2987 0.636364 69.6 rps15-ycf1_p4 rps15-ycf1 4 GCTAGATAATCCCAACGCGT CGAAGAAGAGAGATGCGTCT 20 20 50.0 50.0 57.8 57.2 5.6 8.6 0.992605 142038 143752 895-2986 0.636364 69.5 rps15-ycf1_p5 rps15-ycf1 5 TCAGTCGAAGAACACCATGC TCGAAGAAGAGAGATGCGTC 20 20 50.0 50.0 58.2 57.2 0.0 0.0 1.038807 142038 143752 760-2851 0.636364 69.1 trnI-GAU_p1 trnI-GAU 1 GCATGCATGAAGGAGTCAGA AGAGGGATGGGGTTTCTCTC 20 20 50.0 55.0 58.0 58.1 30.6 0.0 0.147682 155219 156236 1129-1141 1.000000 90.8 trnI-GAU_p2 trnI-GAU 2 GCATGCATGAAGGAGTCAGA GAGAGGGATGGGGTTTCTCT 20 20 50.0 55.0 58.0 58.1 30.6 0.0 0.147682 155219 156236 1130-1142 1.000000 90.8 trnI-GAU_p3 trnI-GAU 3 TGCATGAAGGAGTCAGATGC AGAGAGGGATGGGGTTTCTC 20 20 50.0 55.0 58.0 58.1 0.0 0.0 0.147682 155219 156236 1128-1140 1.000000 90.8 trnI-GAU_p4 trnI-GAU 4 TGCATGAAGGAGTCAGATGC AGAGGGATGGGGTTTCTCTC 20 20 50.0 55.0 58.0 58.1 0.0 0.0 0.147682 155219 156236 1126-1138 1.000000 90.8 trnI-GAU_p5 trnI-GAU 5 TGCATGAAGGAGTCAGATGC GAGAGGGATGGGGTTTCTCT 20 20 50.0 55.0 58.0 58.1 0.0 0.0 0.147682 155219 156236 1127-1139 1.000000 90.8 rps7_p1 rps7 1 TCTTGAACCTCTTTCACGCT CCGATCGAGATGTATGGATCC 20 21 45.0 52.4 57.1 57.6 0.0 18.7 2.339433 161112 161579 546-552 1.000000 73.3 rps7_p2 rps7 2 TTCTTGAACCTCTTTCACGCT CCGATCGAGATGTATGGATCC 21 21 42.9 52.4 57.8 57.6 0.0 18.7 2.622751 161112 161579 547-553 1.000000 71.0 rps7_p3 rps7 3 CTTGAACCTCTTTCACGCTC CCGATCGAGATGTATGGATCC 20 21 50.0 52.4 56.8 57.6 0.0 18.7 2.668721 161112 161579 545-551 1.000000 70.7 rps7_p4 rps7 4 TCTTGAACCTCTTTCACGCTC CCGATCGAGATGTATGGATCC 21 21 47.6 52.4 58.2 57.6 0.0 18.7 2.673096 161112 161579 546-552 1.000000 70.6 rps7_p5 rps7 5 TCTTGAACCTCTTTCACGCT TCGATTCTTTTCCGATCGAGA 20 21 45.0 42.9 57.1 57.0 0.0 18.7 2.960374 161112 161579 557-563 0.909091 64.7 ndhB_p1 ndhB 1 ATTGGGACTTTTTCGGCGAT TAAGTTCATAACGCCAGCCC 20 20 45.0 50.0 58.2 58.0 11.8 0.0 0.198493 161909 164118 2351-2378 1.000000 90.4 ndhB_p2 ndhB 2 CAATTGGGACTTTTTCGGCG TAAGTTCATAACGCCAGCCC 20 20 50.0 50.0 58.3 58.0 0.0 0.0 0.335692 161909 164118 2353-2380 1.000000 89.3 ndhB_p3 ndhB 3 GGCGATTGGATGCAGTTACT TAAGTTCATAACGCCAGCCC 20 20 50.0 50.0 58.3 58.0 0.0 0.0 0.364261 161909 164118 2337-2364 1.000000 89.1 ndhB_p4 ndhB 4 AATTGGGACTTTTTCGGCGA TAAGTTCATAACGCCAGCCC 20 20 45.0 50.0 58.4 58.0 5.0 0.0 0.421162 161909 164118 2352-2379 1.000000 88.6 ndhB_p5 ndhB 5 TTGGGACTTTTTCGGCGATT TAAGTTCATAACGCCAGCCC 20 20 45.0 50.0 58.4 58.0 11.8 0.0 0.421162 161909 164118 2350-2377 1.000000 88.6 ndhB-trnL-CAA_p1 ndhB-trnL-CAA 1 TGCAATTGCTCAGGATACCC GTCTCGCGTGTCTACCATTT 20 20 50.0 50.0 57.9 58.0 29.4 5.8 0.064968 164119 165260 464-2578 0.954545 89.7 ndhB-trnL-CAA_p2 ndhB-trnL-CAA 2 TGCAATTGCTCAGGATACCC CTCGCGTGTCTACCATTTCA 20 20 50.0 50.0 57.9 58.0 29.4 5.8 0.066338 164119 165260 462-2576 0.954545 89.7 ndhB-trnL-CAA_p3 ndhB-trnL-CAA 3 TGCAATTGCTCAGGATACCC GCGTGTCTACCATTTCACCA 20 20 50.0 50.0 57.9 58.2 29.4 0.0 0.253803 164119 165260 459-2573 0.954545 88.2 ndhB-trnL-CAA_p4 ndhB-trnL-CAA 4 TGCAATTGCTCAGGATACCC TCTCGCGTGTCTACCATTTC 20 20 50.0 50.0 57.9 57.7 29.4 5.8 0.330507 164119 165260 463-2577 0.954545 87.5 ndhB-trnL-CAA_p5 ndhB-trnL-CAA 5 TGCAATTGCTCAGGATACCC TCGCGTGTCTACCATTTCAC 20 20 50.0 50.0 57.9 58.3 29.4 5.8 0.351881 164119 165260 461-2575 0.954545 87.4