primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	trnK-UUU-rbcL	1	AGCCGAGTACTCTACCATTGA	GCTTGCGAAGTATGGAAGGA	21	20	47.6	50.0	58.0	58.0	20.3	0.0	1.038479	4335	5248	1003-1004	1.000000	72.7
trnK-UUU-rbcL_p2	trnK-UUU-rbcL	2	AGCCGAGTACTCTACCATTGA	AGTCCTGAATTAGCTGCTGC	21	20	47.6	50.0	58.0	58.0	20.3	13.4	1.049834	4335	5248	1021-1022	1.000000	72.6
trnK-UUU-rbcL_p3	trnK-UUU-rbcL	3	AGCCGAGTACTCTACCATTGA	GAGGCTAGCAAATGGAGTCC	21	20	47.6	55.0	58.0	58.0	20.3	18.2	1.053887	4335	5248	1036-1037	1.000000	72.6
trnK-UUU-rbcL_p4	trnK-UUU-rbcL	4	AGCCGAGTACTCTACCATTGA	TGCTTGCGAAGTATGGAAGG	21	20	47.6	50.0	58.0	58.3	20.3	0.0	1.276465	4335	5248	1004-1005	1.000000	70.8
trnK-UUU-rbcL_p5	trnK-UUU-rbcL	5	AGCCGAGTACTCTACCATTGA	GGCTAGCAAATGGAGTCCTG	21	20	47.6	55.0	58.0	58.3	20.3	18.2	1.341135	4335	5248	1034-1035	1.000000	70.3
ndhJ-trnC-GCA_p1	ndhJ-trnC-GCA	1	TTGGATAGGATGGCCTTTGC	ACTGCAAATCCCTTTTCCCC	20	20	50.0	50.0	57.9	58.4	3.3	0.0	0.427268	12491	13746	1371-1402	1.000000	77.1
ndhJ-trnC-GCA_p2	ndhJ-trnC-GCA	2	TTGGATAGGATGGCCTTTGC	GCAAATCCCTTTTCCCCAGT	20	20	50.0	50.0	57.9	58.4	3.3	0.0	0.427268	12491	13746	1368-1399	1.000000	77.1
ndhJ-trnC-GCA_p3	ndhJ-trnC-GCA	3	TTGGATAGGATGGCCTTTGC	CTGCAAATCCCTTTTCCCCA	20	20	50.0	50.0	57.9	58.4	3.3	0.0	0.428468	12491	13746	1370-1401	1.000000	77.1
ndhJ-trnC-GCA_p4	ndhJ-trnC-GCA	4	TTGGATAGGATGGCCTTTGC	TGCAAATCCCTTTTCCCCAG	20	20	50.0	50.0	57.9	58.4	3.3	0.0	0.428468	12491	13746	1369-1400	1.000000	77.1
ndhJ-trnC-GCA_p5	ndhJ-trnC-GCA	5	TTGGATAGGATGGCCTTTGC	AGTTCAAATCTGGGTGTCGC	20	20	50.0	50.0	57.9	58.5	3.3	0.0	0.548020	12491	13746	1351-1382	1.000000	76.1
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	CTTGTTGAGATCAGGCGACA	CGTCTTTTAAGCCCCGATCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.103151	13821	14576	936-939	1.000000	79.9
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	TTGTTGAGATCAGGCGACAC	CGTCTTTTAAGCCCCGATCA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.222774	13821	14576	935-938	1.000000	79.0
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	CTTGTTGAGATCAGGCGACA	AGTTCGTCTTTTAAGCCCCG	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.280009	13821	14576	940-943	1.000000	78.5
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	CTTGTTGAGATCAGGCGACA	GTTCGTCTTTTAAGCCCCGA	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.285066	13821	14576	939-942	1.000000	78.5
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	GGGGAAAAGGGATTTGCAGT	CGTCTTTTAAGCCCCGATCA	20	20	50.0	50.0	58.4	58.0	0.0	0.0	0.374033	13821	14576	902-905	1.000000	77.8
rpoC2-rps2_p1	rpoC2-rps2	1	TAGGAGCGGATTCATCCCTT	GTGCCATTCTCGGATTCCAT	20	20	50.0	50.0	57.9	58.0	0.0	3.1	0.130281	25014	25089	195-196	1.000000	79.8
rpoC2-rps2_p2	rpoC2-rps2	2	TAGGAGCGGATTCATCCCTT	AAGGTGCCATTCTCGGATTC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.147276	25014	25089	198-199	1.000000	79.6
rpoC2-rps2_p3	rpoC2-rps2	3	TAGGAGCGGATTCATCCCTT	CCGAGTACCATGGCCAAAAT	20	20	50.0	50.0	57.9	58.2	0.0	11.0	0.340446	25014	25089	173-174	1.000000	78.2
rpoC2-rps2_p4	rpoC2-rps2	4	CTAGGAGCGGATTCATCCCT	GTGCCATTCTCGGATTCCAT	20	20	55.0	50.0	58.4	58.0	0.0	3.1	0.388599	25014	25089	196-197	1.000000	77.7
rpoC2-rps2_p5	rpoC2-rps2	5	TCTAGGAGCGGATTCATCCC	GTGCCATTCTCGGATTCCAT	20	20	55.0	50.0	58.4	58.0	0.0	3.1	0.393354	25014	25089	197-198	1.000000	77.7
atpH-atpF_p1	atpH-atpF	1	TGGGCTGGTTGTAGCATTAG	CCAGTAACCCAAGCAAAGGA	20	20	50.0	50.0	57.6	57.7	0.0	0.0	0.704733	27997	28077	171-172	1.000000	75.3
atpH-atpF_p2	atpH-atpF	2	AGGCAGAGGGAAAAATACGG	CCAGTAACCCAAGCAAAGGA	20	20	50.0	50.0	57.6	57.7	0.0	0.0	0.706423	27997	28077	238-239	1.000000	75.0
atpH-atpF_p3	atpH-atpF	3	TGGGCTGGTTGTAGCATTAG	CCGAGCACCACACTTAGATT	20	20	50.0	50.0	57.6	57.6	0.0	0.0	0.805029	27997	28077	245-246	1.000000	74.2
atpH-atpF_p4	atpH-atpF	4	CCTGAGGCAGAGGGAAAAAT	CCAGTAACCCAAGCAAAGGA	20	20	50.0	50.0	57.5	57.7	0.0	0.0	0.807911	27997	28077	242-243	1.000000	74.2
atpH-atpF_p5	atpH-atpF	5	AGGCAGAGGGAAAAATACGG	CCGAGCACCACACTTAGATT	20	20	50.0	50.0	57.6	57.6	0.0	0.0	0.806719	27997	28077	312-313	1.000000	74.1
trnL-UAA_p1	trnL-UAA	1	TCAATTCAACAGTTCTGGCA	AGAGAGTTAACCAGGCCTTT	20	20	40.0	45.0	55.5	56.0	4.8	0.0	4.474454	35223	35831	709-711	1.000000	44.4
trnL-UAA_p2	trnL-UAA	2	ATCAATTCAACAGTTCTGGCA	AGAGAGTTAACCAGGCCTTT	21	20	38.1	45.0	56.1	56.0	4.8	0.0	4.899413	35223	35831	710-712	1.000000	41.0
trnL-UAA_p3	trnL-UAA	3	TCAATTCAACAGTTCTGGCAT	AGAGAGTTAACCAGGCCTTT	21	20	38.1	45.0	56.1	56.0	4.8	0.0	4.899413	35223	35831	709-711	1.000000	41.0
trnL-UAA_p4	trnL-UAA	4	GAATCAATTCAACAGTTCTGGCA	AGAGAGTTAACCAGGCCTTT	23	20	39.1	45.0	57.9	56.0	4.8	0.0	5.078679	35223	35831	712-714	1.000000	40.2
trnL-UAA_p5	trnL-UAA	5	TGAATCAATTCAACAGTTCTGGC	AGAGAGTTAACCAGGCCTTT	23	20	39.1	45.0	57.9	56.0	3.0	0.0	5.078679	35223	35831	713-715	1.000000	40.2
psaA-ycf3_p1	psaA-ycf3	1	TGCCCATTCCTCGAAAGAAG	AGCCCTTACCCCTGGTAATT	20	20	50.0	50.0	57.9	58.0	4.1	6.2	0.117992	50465	51193	877-877	1.000000	79.3
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	AGCCCTTACCCCTGGTAATT	20	20	50.0	50.0	57.9	58.0	4.1	6.2	0.119474	50465	51193	876-876	1.000000	79.3
psaA-ycf3_p3	psaA-ycf3	3	TGCCCATTCCTCGAAAGAAG	AGCTATAGCCCTTACCCCTG	20	20	50.0	55.0	57.9	58.0	4.1	21.2	0.145355	50465	51193	883-883	1.000000	79.1
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	AGCTATAGCCCTTACCCCTG	20	20	50.0	55.0	57.9	58.0	4.1	21.2	0.146838	50465	51193	882-882	1.000000	79.1
psaA-ycf3_p5	psaA-ycf3	5	TGCCCATTCCTCGAAAGAAG	TTGGTTGAAGATCACAGGGC	20	20	50.0	50.0	57.9	58.1	4.1	0.0	0.204161	50465	51193	841-841	1.000000	78.7
psaJ-rpl33_p1	psaJ-rpl33	1	TACTCTATGGTTCGGGGCTT	CGGATATCTTTACCCTTGGCC	20	21	50.0	52.4	57.8	58.2	0.0	0.0	1.390507	66230	66652	537-537	1.000000	69.6
psaJ-rpl33_p2	psaJ-rpl33	2	GCTTTAGCGGGTCTCTTGAT	CGGATATCTTTACCCTTGGCC	20	21	50.0	52.4	57.7	58.2	0.0	0.0	1.538024	66230	66652	521-521	1.000000	68.5
psaJ-rpl33_p3	psaJ-rpl33	3	CTATGGTTCGGGGCTTTAGC	CGGATATCTTTACCCTTGGCC	20	21	55.0	52.4	58.4	58.2	0.0	0.0	1.626164	66230	66652	533-533	1.000000	67.7
psaJ-rpl33_p4	psaJ-rpl33	4	AGTACTCTATGGTTCGGGGC	CGGATATCTTTACCCTTGGCC	20	21	55.0	52.4	58.6	58.2	0.0	0.0	1.809454	66230	66652	539-539	1.000000	66.3
psaJ-rpl33_p5	psaJ-rpl33	5	GTACTCTATGGTTCGGGGCT	CGGATATCTTTACCCTTGGCC	20	21	55.0	52.4	58.6	58.2	0.0	0.0	1.809454	66230	66652	538-538	1.000000	66.3
clpP_p1	clpP	1	CCTTCCTTGCCGTTCGTTAA	ACGTTTCCACATCAAAGTGAGA	20	22	50.0	40.9	58.5	58.2	0.0	0.0	2.680883	69051	71101	2124-2126	1.000000	59.0
clpP_p2	clpP	2	CCTTCCTTGCCGTTCGTTAA	ACGTTTCCACATCAAAGTGAG	20	21	50.0	42.9	58.5	56.8	0.0	0.0	2.727424	69051	71101	2124-2126	1.000000	58.6
clpP_p3	clpP	3	TCCTTGCCGTTCGTTAATCT	ACGTTTCCACATCAAAGTGAGA	20	22	45.0	40.9	57.2	58.2	0.0	0.0	2.950910	69051	71101	2121-2123	1.000000	56.8
clpP_p4	clpP	4	CCTTCCTTGCCGTTCGTTAA	ACGTTTCCACATCAAAGTGA	20	20	50.0	40.0	58.5	55.5	0.0	0.0	2.966331	69051	71101	2124-2126	1.000000	56.7
clpP_p5	clpP	5	TCCTTGCCGTTCGTTAATCT	ACGTTTCCACATCAAAGTGAG	20	21	45.0	42.9	57.2	56.8	0.0	0.0	2.997451	69051	71101	2121-2123	1.000000	56.5
psbB-psbT_p1	psbB-psbT	1	TTTTTGCTGGTATCGACCCA	TGGAACTTTAGGGGGTTCTCT	20	21	45.0	47.6	57.4	58.0	5.6	0.0	1.570487	73112	73310	384-384	1.000000	68.1
psbB-psbT_p2	psbB-psbT	2	GTTTTTGCTGGTATCGACCC	TGGAACTTTAGGGGGTTCTCT	20	21	50.0	47.6	57.1	58.0	0.0	0.0	1.913849	73112	73310	385-385	1.000000	65.3
psbB-psbT_p3	psbB-psbT	3	TGGTATCGACCCAGATTTGG	TGGAACTTTAGGGGGTTCTCT	20	21	50.0	47.6	57.0	58.0	1.6	0.0	2.000457	73112	73310	377-377	1.000000	64.6
psbB-psbT_p4	psbB-psbT	4	TTTTTGCTGGTATCGACCCA	AGTTGGAACTTTAGGGGGTTC	20	21	45.0	47.6	57.4	57.5	5.6	0.0	2.091106	73112	73310	387-387	1.000000	63.9
psbB-psbT_p5	psbB-psbT	5	TTTTTGCTGGTATCGACCCA	GTTGGAACTTTAGGGGGTTCT	20	21	45.0	47.6	57.4	57.5	5.6	0.0	2.091106	73112	73310	386-386	1.000000	63.9
psbT-psbN_p1	psbT-psbN	1	AGAGAACCCCCTAAAGTTCCA	CCATTCGAAGAACATGGGGA	21	20	47.6	50.0	58.0	57.6	0.0	3.3	1.418440	73419	73480	125-125	1.000000	69.9
psbT-psbN_p2	psbT-psbN	2	AGAGAACCCCCTAAAGTTCCA	TCCATTCGAAGAACATGGGG	21	20	47.6	50.0	58.0	57.6	0.0	13.4	1.418440	73419	73480	126-126	1.000000	69.9
psbT-psbN_p3	psbT-psbN	3	AGAGAACCCCCTAAAGTTCCA	GGCAACCCTCTCAACAACTA	21	20	47.6	50.0	58.0	57.4	0.0	0.0	1.561550	73419	73480	151-151	1.000000	68.6
psbT-psbN_p4	psbT-psbN	4	GAACCCCCTAAAGTTCCAACT	CCATTCGAAGAACATGGGGA	21	20	47.6	50.0	57.5	57.6	0.0	3.3	1.939059	73419	73480	122-122	1.000000	65.8
psbT-psbN_p5	psbT-psbN	5	GAACCCCCTAAAGTTCCAACT	TCCATTCGAAGAACATGGGG	21	20	47.6	50.0	57.5	57.6	0.0	13.4	1.939059	73419	73480	123-123	1.000000	65.8
psbH-petB_p1	psbH-petB	1	TAATGGGTGTTGCAATGGCT	TCAGTAACGGTCGGACGATA	20	20	45.0	50.0	58.1	58.0	0.6	1.4	0.086965	73942	75085	1274-1274	1.000000	79.5
psbH-petB_p2	psbH-petB	2	TAATGGGTGTTGCAATGGCT	ATACTTGCCGACCATCGATG	20	20	45.0	50.0	58.1	57.8	0.6	18.8	0.219054	73942	75085	1358-1358	1.000000	78.4
psbH-petB_p3	psbH-petB	3	TAATGGGTGTTGCAATGGCT	ATCATACTTGCCGACCATCG	20	20	45.0	50.0	58.1	57.8	0.6	0.0	0.219054	73942	75085	1361-1361	1.000000	78.4
psbH-petB_p4	psbH-petB	4	TAATGGGTGTTGCAATGGCT	CATACTTGCCGACCATCGAT	20	20	45.0	50.0	58.1	57.8	0.6	0.0	0.219054	73942	75085	1359-1359	1.000000	78.4
psbH-petB_p5	psbH-petB	5	GGGTGTTGCAATGGCTCTAT	TCAGTAACGGTCGGACGATA	20	20	50.0	50.0	58.2	58.0	0.6	1.4	0.255006	73942	75085	1270-1270	1.000000	78.1
rps11-rpl36_p1	rps11-rpl36	1	CGTCCATTTTTACGCGAACC	AGGAGGGGGCGAATTATAGT	20	20	50.0	50.0	58.1	57.6	15.9	0.0	0.544424	78622	78953	436-436	1.000000	75.8
rps11-rpl36_p2	rps11-rpl36	2	TTACGCGAACCAATACGTCC	AGGAGGGGGCGAATTATAGT	20	20	50.0	50.0	58.4	57.6	15.9	0.0	0.789840	78622	78953	451-451	1.000000	73.9
rps11-rpl36_p3	rps11-rpl36	3	ACGTCCATTTTTACGCGAAC	AGGAGGGGGCGAATTATAGT	20	20	45.0	50.0	57.4	57.6	15.9	0.0	1.000165	78622	78953	437-437	1.000000	72.2
rps11-rpl36_p4	rps11-rpl36	4	CGTCCATTTTTACGCGAACC	AAGGGCCTCTGTTCGTAAAA	20	20	50.0	45.0	58.1	57.1	15.9	6.5	1.069741	78622	78953	482-482	1.000000	71.6
rps11-rpl36_p5	rps11-rpl36	5	CGTCCATTTTTACGCGAACC	CGTAGGAGGGGGCGAATTAT	20	20	50.0	55.0	58.1	59.0	15.9	0.0	1.152322	78622	78953	439-439	1.000000	71.0
rpl36-rps8_p1	rpl36-rps8	1	CCCTCCTACGGATCAATCGA	AATGACAGATCGAGAGGCTC	20	20	55.0	50.0	58.4	56.8	0.0	6.3	1.558334	79068	79550	608-648	1.000000	68.3
rpl36-rps8_p2	rpl36-rps8	2	ACTATAATTCGCCCCCTCCT	AATGACAGATCGAGAGGCTC	20	20	50.0	50.0	57.6	56.8	0.0	6.3	1.593375	79068	79550	621-661	1.000000	68.0
rpl36-rps8_p3	rpl36-rps8	3	CCTCCTACGGATCAATCGAC	AATGACAGATCGAGAGGCTC	20	20	55.0	50.0	57.3	56.8	0.0	6.3	1.884043	79068	79550	607-647	1.000000	65.7
rpl36-rps8_p4	rpl36-rps8	4	TTTTACGAACAGAGGCCCTT	AATGACAGATCGAGAGGCTC	20	20	45.0	50.0	57.1	56.8	0.0	6.3	2.118692	79068	79550	575-615	1.000000	63.9
rpl36-rps8_p5	rpl36-rps8	5	CCCTCCTACGGATCAATCGA	ACAAAGAATCGGTGGAGAAGT	20	21	55.0	42.9	58.4	57.2	0.0	0.0	2.154255	79068	79550	584-624	1.000000	63.6
rpl16-rps3_p1	rpl16-rps3	1	CTCAAGTGCCTGAAGAGCAT	TCCTCTACAAACCATTCGAGC	20	21	50.0	47.6	57.9	57.8	0.0	0.0	1.357907	81066	82337	1444-1449	1.000000	69.5
rpl16-rps3_p2	rpl16-rps3	2	CTCAAGTGCCTGAAGAGCAT	CCTCTACAAACCATTCGAGCT	20	21	50.0	47.6	57.9	57.7	0.0	0.0	1.363875	81066	82337	1443-1448	1.000000	69.4
rpl16-rps3_p3	rpl16-rps3	3	CTAGATGTGATCCAAGCGGG	TCCTCTACAAACCATTCGAGC	20	21	55.0	47.6	57.8	57.8	0.0	0.0	1.407828	81066	82337	1464-1469	1.000000	69.1
rpl16-rps3_p4	rpl16-rps3	4	CTAGATGTGATCCAAGCGGG	CCTCTACAAACCATTCGAGCT	20	21	55.0	47.6	57.8	57.7	0.0	0.0	1.413796	81066	82337	1463-1468	1.000000	69.0
rpl16-rps3_p5	rpl16-rps3	5	CTCAAGTGCCTGAAGAGCAT	GGTAGGGTTCCTCTACAAACC	20	21	50.0	52.4	57.9	57.4	0.0	14.6	1.734808	81066	82337	1452-1457	1.000000	66.5
rps19-rpl2_p1	rps19-rpl2	1	TGGTAGATGTTCTTGACCAAGT	GGGCCCCAATTGGTAGAAAA	22	20	40.9	50.0	57.0	58.1	9.3	22.9	3.029065	83832	83906	310-312	1.000000	56.5
rps19-rpl2_p2	rps19-rpl2	2	TGGTAGATGTTCTTGACCAAGT	TTGATTCTTCGTCGCCGTAG	22	20	40.9	50.0	57.0	58.1	9.3	0.0	3.070501	83832	83906	218-220	1.000000	56.5
rps19-rpl2_p3	rps19-rpl2	3	TGGTAGATGTTCTTGACCAAGT	TTGGGGTTATCCTGCACTTG	22	20	40.9	50.0	57.0	57.8	9.3	0.0	3.189021	83832	83906	276-278	1.000000	55.4
rps19-rpl2_p4	rps19-rpl2	4	TGGTAGATGTTCTTGACCAAGT	GCAACTCCTTGGGGTTATCC	22	20	40.9	55.0	57.0	58.2	9.3	4.4	3.210677	83832	83906	284-286	1.000000	55.2
rps19-rpl2_p5	rps19-rpl2	5	TCTTTTGCTGCTTTTGTGTT	GGGCCCCAATTGGTAGAAAA	20	20	35.0	50.0	54.8	58.1	0.0	22.9	3.244732	83832	83906	278-280	1.000000	54.9
ycf2_p1	ycf2	1	CCCTTTTCGCTCCGCTTAG	CAGCTCCGTATCAAGGTCAC	19	20	57.9	55.0	58.6	58.1	0.0	0.0	1.687902	91394	91513	213-276	1.000000	70.4
ycf2_p2	ycf2	2	CTGGACGATCCTATTTGGTCA	CAGCTCCGTATCAAGGTCAC	21	20	47.6	55.0	57.2	58.1	0.0	0.0	1.891669	91394	91513	149-212	1.000000	70.2
ycf2_p3	ycf2	3	GGGAAACCCTTTTCGCTCC	CAGCTCCGTATCAAGGTCAC	19	20	57.9	55.0	58.7	58.1	6.9	0.0	1.807981	91394	91513	219-282	1.000000	69.4
ycf2_p4	ycf2	4	CCCTTTTCGCTCCGCTTAG	CAAGGTCACGGTCGATATCG	19	20	57.9	55.0	58.6	58.2	0.0	19.1	1.860636	91394	91513	202-265	1.000000	69.2
ycf2_p5	ycf2	5	CCCTTTTCGCTCCGCTTAG	GTCACGGTCGATATCGTCAC	19	20	57.9	55.0	58.6	58.3	0.0	23.6	1.876179	91394	91513	198-261	1.000000	69.2