primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	GGAATCCTGGGCGTAAAGAA	GGGTTCGAATCCCTCTCTCT	20	20	50.0	55.0	57.6	58.2	0.0	17.2	0.630238	8753	8810	102-104	1.000000	79.6
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	GGAATCCTGGGCGTAAAGAA	TTCGAATCCCTCTCTCTCCG	20	20	50.0	55.0	57.6	58.3	0.0	0.0	0.731772	8753	8810	99-101	1.000000	79.0
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	GGAATCCTGGGCGTAAAGAA	GACTAAAGCGGTGGATTGCT	20	20	50.0	50.0	57.6	58.3	0.0	0.0	0.674243	8753	8810	150-152	1.000000	77.8
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	GGAATCCTGGGCGTAAAGAA	GATGGCTGAGCGGACTAAAG	20	20	50.0	55.0	57.6	58.4	0.0	0.0	0.836954	8753	8810	162-164	1.000000	76.3
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	TTATCGAACGATCCAGGACG	GGGTTCGAATCCCTCTCTCT	20	20	50.0	55.0	57.2	58.2	12.9	17.2	0.985762	8753	8810	121-123	1.000000	76.1
atpH-atpI_p1	atpH-atpI	1	TCCAATAGAAGCGAGGCCTA	CGAATCCATGGAGGGTCATC	20	20	50.0	55.0	57.9	57.8	4.4	21.3	0.258018	14154	14826	766-768	1.000000	79.0
atpH-atpI_p2	atpH-atpI	2	CCTACGGATAATCCAGCAGC	CGAATCCATGGAGGGTCATC	20	20	55.0	55.0	57.9	57.8	0.0	21.3	0.261932	14154	14826	750-752	1.000000	79.0
atpH-atpI_p3	atpH-atpI	3	GCCTACGGATAATCCAGCAG	CGAATCCATGGAGGGTCATC	20	20	55.0	55.0	57.9	57.8	0.0	21.3	0.261932	14154	14826	751-753	1.000000	79.0
atpH-atpI_p4	atpH-atpI	4	AACGGAAGCAGCAGAAATCA	CGAATCCATGGAGGGTCATC	20	20	45.0	55.0	58.1	57.8	0.0	21.3	0.279658	14154	14826	727-729	1.000000	78.9
atpH-atpI_p5	atpH-atpI	5	ACGGAAGCAGCAGAAATCAA	CGAATCCATGGAGGGTCATC	20	20	45.0	55.0	58.1	57.8	0.0	21.3	0.279658	14154	14826	726-728	1.000000	78.9
petN-psbM_p1	petN-psbM	1	GGCTGCTCTAATGGTCGTTT	ACGGTGAGTCAAAGCAATTG	20	20	50.0	45.0	58.3	56.9	0.0	0.0	1.326257	27486	28359	953-963	1.000000	70.9
petN-psbM_p2	petN-psbM	2	CTTGGGCTGCTCTAATGGTC	ACGGTGAGTCAAAGCAATTG	20	20	55.0	45.0	58.3	56.9	0.0	0.0	1.392156	27486	28359	957-967	1.000000	70.4
petN-psbM_p3	petN-psbM	3	GGCTGCTCTAATGGTCGTTT	CGGTGAGTCAAAGCAATTGA	20	20	50.0	45.0	58.3	56.7	0.0	10.3	1.609876	27486	28359	952-962	1.000000	68.6
petN-psbM_p4	petN-psbM	4	GGCTGCTCTAATGGTCGTTT	AACGGTGAGTCAAAGCAATTG	20	21	50.0	42.9	58.3	57.6	0.0	0.0	1.615022	27486	28359	954-964	1.000000	68.6
petN-psbM_p5	petN-psbM	5	CTTGGGCTGCTCTAATGGTC	CGGTGAGTCAAAGCAATTGA	20	20	55.0	45.0	58.3	56.7	0.0	10.3	1.675774	27486	28359	956-966	1.000000	68.1
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	CAATTGCTTTGACTCACCGT	TTAGAGTACCGCCCTGTCAA	20	20	45.0	50.0	56.9	58.1	0.0	0.0	1.137837	28465	29805	1492-1502	1.000000	72.0
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	CAATTGCTTTGACTCACCGT	ATTGGTTAGAGTACCGCCCT	20	20	45.0	50.0	56.9	58.1	0.0	0.0	1.189836	28465	29805	1497-1507	1.000000	71.5
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	TCAATTGCTTTGACTCACCG	TTAGAGTACCGCCCTGTCAA	20	20	45.0	50.0	56.7	58.1	0.0	0.0	1.421455	28465	29805	1493-1503	1.000000	69.7
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	CAATTGCTTTGACTCACCGTT	TTAGAGTACCGCCCTGTCAA	21	20	42.9	50.0	57.6	58.1	0.0	0.0	1.426602	28465	29805	1492-1502	1.000000	69.7
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	TCAATTGCTTTGACTCACCG	ATTGGTTAGAGTACCGCCCT	20	20	45.0	50.0	56.7	58.1	0.0	0.0	1.473454	28465	29805	1498-1508	1.000000	69.3
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	TGCAGTAGGAGGAAAATCCG	TGGCACGAGGATTTTCAGTC	20	20	50.0	50.0	57.3	58.2	0.0	0.0	0.882998	47428	47831	514-516	1.000000	74.2
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	GCAGTAGGAGGAAAATCCGT	TGGCACGAGGATTTTCAGTC	20	20	50.0	50.0	57.3	58.2	0.0	0.0	0.884856	47428	47831	513-515	1.000000	74.2
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	TGCAGTAGGAGGAAAATCCG	AATAATGGTTCCGCATGCCA	20	20	50.0	45.0	57.3	58.2	0.0	13.2	0.917299	47428	47831	553-555	1.000000	73.8
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	GCAGTAGGAGGAAAATCCGT	AATAATGGTTCCGCATGCCA	20	20	50.0	45.0	57.3	58.2	0.0	13.2	0.919157	47428	47831	552-554	1.000000	73.8
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	TGCAGTAGGAGGAAAATCCG	CCAGATTTGAACTGGTGGCA	20	20	50.0	50.0	57.3	58.4	0.0	0.0	1.071755	47428	47831	529-531	1.000000	72.6
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TTACTTATGGGGGCCAAAGC	TGGGTTCTTGGAACAGTTCG	20	20	50.0	50.0	57.8	58.0	4.4	7.2	0.191240	50032	52002	2164-2169	1.000000	79.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TTACTTATGGGGGCCAAAGC	TGCATGTTGGGTTCTTGGAA	20	20	50.0	45.0	57.8	57.9	4.4	0.0	0.232203	50032	52002	2171-2176	1.000000	79.1
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GGGGCCAAAGCTATGGAAAT	TGGGTTCTTGGAACAGTTCG	20	20	50.0	50.0	58.2	58.0	4.4	7.2	0.244793	50032	52002	2155-2160	1.000000	79.0
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGGGCCAAAGCTATGGAAAT	TGCATGTTGGGTTCTTGGAA	20	20	50.0	45.0	58.2	57.9	4.4	0.0	0.285756	50032	52002	2162-2167	1.000000	78.6
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CTCCGGCCCTTTACTTATGG	TGGGTTCTTGGAACAGTTCG	20	20	55.0	50.0	57.7	58.0	0.0	7.2	0.294324	50032	52002	2174-2179	1.000000	78.6
accD_p1	accD	1	TCGATTCACGAGGTTCTGAT	GGATCAAGACTTGTTTTTGGGG	20	22	45.0	45.5	56.4	57.7	0.0	14.7	3.873387	57839	60565	2883-2979	0.833333	44.5
accD_p2	accD	2	TGTGCAAAGGATATCACTCGA	GGATCAAGACTTGTTTTTGGGG	21	22	42.9	45.5	57.1	57.7	4.8	14.7	4.142991	57839	60565	2900-2996	0.833333	42.3
accD_p3	accD	3	CGAGTTCTTCAAGTCGTTCCA	GGATCAAGACTTGTTTTTGGGG	21	22	47.6	45.5	58.3	57.7	4.2	14.7	3.517975	57839	60565	2948-2996	0.500000	33.6
accD_p4	accD	4	CGAGTTCTTCAAGTCGTTCC	GGATCAAGACTTGTTTTTGGGG	20	22	50.0	45.5	56.5	57.7	4.2	14.7	3.747898	57839	60565	2948-2996	0.500000	31.8
accD_p5	accD	5	CGAGTTCTTCAAGTCGTTCCA	TCAAGACTTGTTTTTGGGGA	21	20	47.6	40.0	58.3	55.0	4.2	14.7	4.283170	57839	60565	2945-2993	0.500000	27.5
clpP-rps12_p1	clpP-rps12	1	AATTTGGCCTTGTCGACCAA	TTCTGTATTGGCTGTCTCGC	20	20	45.0	50.0	58.3	58.0	14.1	0.0	0.311639	61900	62507	768-774	1.000000	80.9
clpP-rps12_p2	clpP-rps12	2	TTTGGCCTTGTCGACCAAAT	TTCTGTATTGGCTGTCTCGC	20	20	45.0	50.0	58.3	58.0	14.1	0.0	0.311639	61900	62507	766-772	1.000000	80.9
clpP-rps12_p3	clpP-rps12	3	AATTTGGCCTTGTCGACCAA	CCTTTACGCTGAGGACATCC	20	20	45.0	55.0	58.3	58.1	14.1	0.0	0.360169	61900	62507	813-819	1.000000	80.4
clpP-rps12_p4	clpP-rps12	4	TTTGGCCTTGTCGACCAAAT	CCTTTACGCTGAGGACATCC	20	20	45.0	55.0	58.3	58.1	14.1	0.0	0.360169	61900	62507	811-817	1.000000	80.4
clpP-rps12_p5	clpP-rps12	5	AGAATTTGGCCTTGTCGACC	TTCTGTATTGGCTGTCTCGC	20	20	50.0	50.0	58.5	58.0	14.1	0.0	0.475416	61900	62507	770-776	1.000000	79.6
psbJ-petA_p1	psbJ-petA	1	TCCGGATTAGGATCATCCCT	TAGCATCTGTCGTTTTGGCA	20	20	50.0	45.0	57.0	57.8	0.0	0.0	1.200037	68202	69210	1112-1123	1.000000	72.1
psbJ-petA_p2	psbJ-petA	2	TCCGGATTAGGATCATCCCT	TCTGTCGTTTTGGCACAGAT	20	20	50.0	45.0	57.0	57.7	0.0	0.0	1.277189	68202	69210	1107-1118	1.000000	71.5
psbJ-petA_p3	psbJ-petA	3	TCCGGATTAGGATCATCCCT	CATCTGTCGTTTTGGCACAG	20	20	50.0	50.0	57.0	57.7	0.0	0.0	1.343449	68202	69210	1109-1120	1.000000	71.0
psbJ-petA_p4	psbJ-petA	4	TTCCGGATTAGGATCATCCCT	TAGCATCTGTCGTTTTGGCA	21	20	47.6	45.0	57.7	57.8	4.1	0.0	1.453242	68202	69210	1113-1124	1.000000	70.1
psbJ-petA_p5	psbJ-petA	5	TTCCGGATTAGGATCATCCCT	TCTGTCGTTTTGGCACAGAT	21	20	47.6	45.0	57.7	57.7	4.1	0.0	1.530393	68202	69210	1108-1119	1.000000	69.5
ycf2-trnL-CAA_p1	ycf2-trnL-CAA	1	TCGGAATGACATTCACTGCT	AAATGGTAGACACGCGAGAC	20	20	45.0	50.0	57.2	58.0	25.6	15.9	0.776083	94888	95467	662-707	1.000000	78.6
ycf2-trnL-CAA_p2	ycf2-trnL-CAA	2	TCGGAATGACATTCACTGCT	TGAAATGGTAGACACGCGAG	20	20	45.0	50.0	57.2	58.0	25.6	15.9	0.777454	94888	95467	664-709	1.000000	78.6
ycf2-trnL-CAA_p3	ycf2-trnL-CAA	3	ATGGCTTTCTCGGAATGACA	AAATGGTAGACACGCGAGAC	20	20	45.0	50.0	57.2	58.0	9.4	15.9	0.795303	94888	95467	671-716	1.000000	78.4
ycf2-trnL-CAA_p4	ycf2-trnL-CAA	4	TGGCTTTCTCGGAATGACAT	AAATGGTAGACACGCGAGAC	20	20	45.0	50.0	57.2	58.0	4.6	15.9	0.795303	94888	95467	670-715	1.000000	78.4
ycf2-trnL-CAA_p5	ycf2-trnL-CAA	5	TGGCTTTCTCGGAATGACAT	TGAAATGGTAGACACGCGAG	20	20	45.0	50.0	57.2	58.0	4.6	15.9	0.796674	94888	95467	672-717	1.000000	78.4
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	TGGCAACTTTACGTAAGGCA	GACTTTCACCACGTCAAGGT	20	20	45.0	50.0	57.7	58.1	16.5	0.0	0.308225	99874	100861	1071-1081	1.000000	79.1
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	TGGCAACTTTACGTAAGGCA	TGACTTTCACCACGTCAAGG	20	20	45.0	50.0	57.7	58.1	16.5	0.0	0.309586	99874	100861	1072-1082	1.000000	79.1
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	TGGCAACTTTACGTAAGGCA	CGATGACTTTCACCACGTCA	20	20	45.0	50.0	57.7	58.2	16.5	0.0	0.479356	99874	100861	1075-1085	1.000000	77.7
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	ACGTAAGGCAGAGTTTGGTT	GACTTTCACCACGTCAAGGT	20	20	45.0	50.0	57.4	58.1	0.0	0.0	0.683521	99874	100861	1061-1071	1.000000	76.1
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	ACGTAAGGCAGAGTTTGGTT	TGACTTTCACCACGTCAAGG	20	20	45.0	50.0	57.4	58.1	0.0	0.0	0.684882	99874	100861	1062-1072	1.000000	76.1
trnV-GAC-rrn16_p1	trnV-GAC-rrn16	1	ACCTTGACGTGGTGAAAGTC	CCGACTTGCATGTGTTAAGC	20	20	50.0	50.0	58.1	57.8	0.0	10.4	0.297845	100934	102313	1107-1491	1.000000	84.0
trnV-GAC-rrn16_p2	trnV-GAC-rrn16	2	CCTTGACGTGGTGAAAGTCA	CCGACTTGCATGTGTTAAGC	20	20	50.0	50.0	58.1	57.8	0.0	10.4	0.299207	100934	102313	1106-1490	1.000000	84.0
trnV-GAC-rrn16_p3	trnV-GAC-rrn16	3	TGACGTGGTGAAAGTCATCG	CCGACTTGCATGTGTTAAGC	20	20	50.0	50.0	58.2	57.8	0.0	10.4	0.468976	100934	102313	1103-1487	1.000000	82.7
trnV-GAC-rrn16_p4	trnV-GAC-rrn16	4	ACCTTGACGTGGTGAAAGTC	CGTCCGACTTGCATGTGTTA	20	20	50.0	50.0	58.1	58.6	0.0	10.4	0.626132	100934	102313	1110-1494	1.000000	81.4
trnV-GAC-rrn16_p5	trnV-GAC-rrn16	5	CCTTGACGTGGTGAAAGTCA	CGTCCGACTTGCATGTGTTA	20	20	50.0	50.0	58.1	58.6	0.0	10.4	0.627493	100934	102313	1109-1493	1.000000	81.4
rrn4.5-rrn5_p1	rrn4.5-rrn5	1	CAGCTGAGGCATCCTAACAG	GCATCGAGCTATTTTTCCGC	20	20	55.0	50.0	58.0	58.0	8.4	0.0	0.073347	109322	109661	490-493	1.000000	80.9
rrn4.5-rrn5_p2	rrn4.5-rrn5	2	GCTGAGGCATCCTAACAGAC	GCATCGAGCTATTTTTCCGC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.077431	109322	109661	488-491	1.000000	80.9
rrn4.5-rrn5_p3	rrn4.5-rrn5	3	CAGCTGAGGCATCCTAACAG	AGTATCGTCACCGCAGTAGA	20	20	55.0	50.0	58.0	58.0	8.4	0.0	0.083276	109322	109661	456-459	1.000000	80.9
rrn4.5-rrn5_p4	rrn4.5-rrn5	4	CAGCTGAGGCATCCTAACAG	TATCGTCACCGCAGTAGAGT	20	20	55.0	50.0	58.0	58.0	8.4	0.0	0.083276	109322	109661	454-457	1.000000	80.9
rrn4.5-rrn5_p5	rrn4.5-rrn5	5	GCTGAGGCATCCTAACAGAC	TATCGTCACCGCAGTAGAGT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.087359	109322	109661	452-455	1.000000	80.9
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	20	20	55.0	50.0	57.8	57.7	18.9	0.0	0.526686	110071	110544	609-609	1.000000	76.6
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	CAGAGGATTAGAGCACGTGG	CCGATTGGTCGTAGGTTCAA	20	20	55.0	50.0	57.8	57.6	18.9	0.0	0.589447	110071	110544	571-571	1.000000	76.1
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	TTTTTGGAGGCACTTCTGGG	TTCCTCAGTAGCTCAGTGGT	20	20	50.0	50.0	58.3	57.7	0.0	0.0	0.607368	110071	110544	665-665	1.000000	75.9
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	TTTTTGGAGGCACTTCTGGG	CCGATTGGTCGTAGGTTCAA	20	20	50.0	50.0	58.3	57.6	0.0	0.0	0.670130	110071	110544	627-627	1.000000	75.4
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	GAACAGATTCCGTGGAGACG	TTCCTCAGTAGCTCAGTGGT	20	20	55.0	50.0	58.4	57.7	0.0	0.0	0.680631	110071	110544	641-641	1.000000	75.3
ycf1-chlN_p1	ycf1-chlN	1	CAAGGTCGTCAAGAACCCAT	GAAGACTTGGATCGGACCAC	20	20	50.0	55.0	57.8	58.0	0.0	0.0	0.196544	132189	133117	1105-1147	1.000000	80.7
ycf1-chlN_p2	ycf1-chlN	2	CAAGGTCGTCAAGAACCCAT	AATTGGTTACCCGACCCCTA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.215291	132189	133117	1143-1185	1.000000	80.4
ycf1-chlN_p3	ycf1-chlN	3	CAAGGTCGTCAAGAACCCAT	ACTTGGATCGGACCACCTTA	20	20	50.0	50.0	57.8	58.1	0.0	1.3	0.238020	132189	133117	1101-1143	1.000000	80.3
ycf1-chlN_p4	ycf1-chlN	4	CAAGGTCGTCAAGAACCCAT	TTGGATCGGACCACCTTAGT	20	20	50.0	50.0	57.8	58.1	0.0	1.3	0.238020	132189	133117	1099-1141	1.000000	80.3
ycf1-chlN_p5	ycf1-chlN	5	CAAGGTCGTCAAGAACCCAT	AGAAGACTTGGATCGGACCA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.246419	132189	133117	1106-1148	1.000000	80.3
chlL-trnH-GUG_p1	chlL-trnH-GUG	1	ACCAATTCCGCCTTTTCCAT	ATTATTTGGGGCGGACGTAG	20	20	45.0	50.0	58.1	57.8	0.0	0.0	0.312652	135414	136090	798-814	0.333333	51.0
chlL-trnH-GUG_p2	chlL-trnH-GUG	2	ACCAATTCCGCCTTTTCCAT	CTATTATTTGGGGCGGACGT	20	20	45.0	50.0	58.1	57.8	0.0	0.0	0.312652	135414	136090	800-816	0.333333	51.0
chlL-trnH-GUG_p3	chlL-trnH-GUG	3	GTCTGGCTAGGGCAATTGAA	ATTATTTGGGGCGGACGTAG	20	20	50.0	50.0	57.9	57.8	10.3	0.0	0.378614	135414	136090	841-857	0.333333	50.5
chlL-trnH-GUG_p4	chlL-trnH-GUG	4	GTCTGGCTAGGGCAATTGAA	CTATTATTTGGGGCGGACGT	20	20	50.0	50.0	57.9	57.8	10.3	0.0	0.378614	135414	136090	843-859	0.333333	50.5
chlL-trnH-GUG_p5	chlL-trnH-GUG	5	TTTTCACCACGTCTGGCTAG	ATTATTTGGGGCGGACGTAG	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.413662	135414	136090	851-867	0.333333	50.2