primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCGCACTTAAAAGCCGAGTA	GGGCGGAGGTTTTTAAGGAA	20	20	50.0	50.0	58.3	58.1	5.3	0.0	0.369028	4174	5022	859-966	1.000000	86.3
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCGCACTTAAAAGCCGAGTA	CAAGAGGGCGGAGGTTTTTA	20	20	50.0	50.0	58.3	57.8	5.3	0.0	0.477008	4174	5022	864-971	1.000000	85.4
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCGCACTTAAAAGCCGAGTA	AGAGGGCGGAGGTTTTTAAG	20	20	50.0	50.0	58.3	57.5	5.3	0.0	0.774481	4174	5022	862-969	1.000000	83.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGCACTTAAAAGCCGAGTA	GAGGGCGGAGGTTTTTAAGG	20	20	50.0	55.0	58.3	58.5	5.3	0.0	0.822302	4174	5022	861-968	1.000000	82.7
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CGAATTCCTTGCTTCATCCG	GGGCGGAGGTTTTTAAGGAA	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.831009	4174	5022	898-1005	1.000000	82.4
rps16_p1	rps16	1	TTGTGTGTTGGATTGGCACT	TGACTGTCCTGACTGAACCA	20	20	45.0	50.0	58.2	58.2	0.0	5.2	0.437661	5023	6135	1310-1324	1.000000	82.2
rps16_p2	rps16	2	TTTGTGTGTTGGATTGGCAC	TGACTGTCCTGACTGAACCA	20	20	45.0	50.0	57.4	58.2	0.0	5.2	0.805117	5023	6135	1311-1325	1.000000	79.3
rps16_p3	rps16	3	TGTGTGTTGGATTGGCACTA	TGACTGTCCTGACTGAACCA	20	20	45.0	50.0	57.4	58.2	0.0	5.2	0.859454	5023	6135	1309-1323	1.000000	78.8
rps16_p4	rps16	4	TGTGTTGGATTGGCACTACA	TGACTGTCCTGACTGAACCA	20	20	45.0	50.0	57.4	58.2	0.0	5.2	0.859454	5023	6135	1307-1321	1.000000	78.8
rps16_p5	rps16	5	TTGTGTGTTGGATTGGCACT	ACCGGTTCCAAATTTGAGGA	20	20	45.0	45.0	58.2	57.3	0.0	19.9	0.894034	5023	6135	1253-1267	1.000000	78.6
trnS-trnG-UCC_p1	trnS-trnG-UCC	1	ACGGAAAGAGAGGGATTCGA	TCAAACCGGTAGACCCCTTA	20	20	50.0	50.0	58.2	57.7	0.0	8.0	0.475347	8062	8718	788-851	1.000000	85.0
trnS-trnG-UCC_p2	trnS-trnG-UCC	2	ACGGAAAGAGAGGGATTCGA	AACCGGTAGACCCCTTAACT	20	20	50.0	50.0	58.2	57.7	0.0	8.0	0.483680	8062	8718	785-848	1.000000	84.9
trnS-trnG-UCC_p3	trnS-trnG-UCC	3	ACGGAAAGAGAGGGATTCGA	AATCAAACCGGTAGACCCCT	20	20	50.0	50.0	58.2	58.3	0.0	8.0	0.486211	8062	8718	790-853	1.000000	84.9
trnS-trnG-UCC_p4	trnS-trnG-UCC	4	ACGGAAAGAGAGGGATTCGA	ATCAAACCGGTAGACCCCTT	20	20	50.0	50.0	58.2	58.3	0.0	8.0	0.486211	8062	8718	789-852	1.000000	84.9
trnS-trnG-UCC_p5	trnS-trnG-UCC	5	TGGAAACGGAAAGAGAGGGA	TCAAACCGGTAGACCCCTTA	20	20	50.0	50.0	58.3	57.7	0.0	8.0	0.599454	8062	8718	793-856	1.000000	83.9
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTTGCAACTTTAGCTGCAGC	20	20	45.0	45.0	58.0	57.9	1.4	31.5	0.176983	13467	14214	822-837	1.000000	83.3
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	20	20	45.0	55.0	58.0	57.8	1.4	21.3	0.214265	13467	14214	793-808	1.000000	83.2
atpH-atpI_p3	atpH-atpI	3	AGCGGCAGAAATCAATGGAT	TTTGCAACTTTAGCTGCAGC	20	20	45.0	45.0	57.9	57.9	1.4	31.5	0.201567	13467	14214	814-829	1.000000	83.1
atpH-atpI_p4	atpH-atpI	4	AGCGGCAGAAATCAATGGAT	CGAATCCATGGAGGGTCATC	20	20	45.0	55.0	57.9	57.8	1.4	21.3	0.238848	13467	14214	785-800	1.000000	83.0
atpH-atpI_p5	atpH-atpI	5	TACCTTGACCAACTCCAGGT	TTTGCAACTTTAGCTGCAGC	20	20	50.0	45.0	57.9	57.9	0.0	31.5	0.250875	13467	14214	878-893	1.000000	82.4
trnC-GCA-petN_p1	trnC-GCA-petN	1	TGTTCCCCAGTTCAAATCCG	GCCCAAGCGAGACTTACAAT	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.356647	27642	28143	548-577	1.000000	82.4
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTTGTATCGTTTTGGCGGC	GCCCAAGCGAGACTTACAAT	20	20	45.0	50.0	57.9	58.3	0.0	0.0	0.314215	27642	28143	603-632	1.000000	82.3
trnC-GCA-petN_p3	trnC-GCA-petN	3	TGTTCCCCAGTTCAAATCCG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.446576	27642	28143	610-639	1.000000	81.2
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTTGTATCGTTTTGGCGGC	AGAGTCCACTTCTTCCCCAT	20	20	45.0	50.0	57.9	57.6	0.0	0.0	0.404145	27642	28143	665-694	1.000000	81.1
trnC-GCA-petN_p5	trnC-GCA-petN	5	GCAAATCCTTGTTCCCCAGT	GCCCAAGCGAGACTTACAAT	20	20	50.0	50.0	58.4	58.3	0.0	0.0	0.628345	27642	28143	557-586	1.000000	80.2
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	TCATTTTGGCTGACCGTTTT	TCAATTGGTTAGAGCACCGC	20	20	40.0	50.0	56.4	58.6	0.0	5.4	2.108386	29233	30306	872-1272	1.000000	71.8
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AATCATTTTGGCTGACCGTT	TCAATTGGTTAGAGCACCGC	20	20	40.0	50.0	56.2	58.6	0.0	5.4	2.339357	29233	30306	874-1274	1.000000	69.9
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	ATCATTTTGGCTGACCGTTT	TCAATTGGTTAGAGCACCGC	20	20	40.0	50.0	56.2	58.6	0.0	5.4	2.339357	29233	30306	873-1273	1.000000	69.9
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	TCATTTTGGCTGACCGTTTTT	TCAATTGGTTAGAGCACCGC	21	20	38.1	50.0	57.2	58.6	0.0	5.4	2.361326	29233	30306	872-1272	1.000000	69.8
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	TTTGGCTGACCGTTTTTACA	TCAATTGGTTAGAGCACCGC	20	20	40.0	50.0	56.1	58.6	0.0	5.4	2.457955	29233	30306	868-1268	1.000000	69.0
trnE-UUC-trnT_p1	trnE-UUC-trnT	1	GAATCCCCGTTGTCTCCTTG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.266904	31001	31494	601-638	1.000000	82.5
trnE-UUC-trnT_p2	trnE-UUC-trnT	2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	31001	31494	572-609	1.000000	82.5
trnE-UUC-trnT_p3	trnE-UUC-trnT	3	GAATCCCCGTTGTCTCCTTG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.325001	31001	31494	580-617	1.000000	82.2
trnE-UUC-trnT_p4	trnE-UUC-trnT	4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.345606	31001	31494	551-588	1.000000	82.2
trnE-UUC-trnT_p5	trnE-UUC-trnT	5	GAATCCCCGTTGTCTCCTTG	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.347155	31001	31494	577-614	1.000000	82.0
psaA-ycf3_p1	psaA-ycf3	1	TGGCCCATTCTTCAAACGAA	ATCAAGCCGCTGAGTATTGG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.052665	42004	42773	933-974	1.000000	84.9
psaA-ycf3_p2	psaA-ycf3	2	TGGCCCATTCTTCAAACGAA	ATTGGTTGAAGATCACGGGG	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.140477	42004	42773	867-908	1.000000	84.6
psaA-ycf3_p3	psaA-ycf3	3	GGTCTGGCCCATTCTTCAAA	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.117603	42004	42773	937-978	1.000000	84.4
psaA-ycf3_p4	psaA-ycf3	4	TGGCCCATTCTTCAAACGAA	CGATCAAGCCGCTGAGTATT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.153615	42004	42773	935-976	1.000000	84.1
psaA-ycf3_p5	psaA-ycf3	5	GGTCTGGCCCATTCTTCAAA	ATTGGTTGAAGATCACGGGG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.205415	42004	42773	871-912	1.000000	84.1
rps4-trnT_p1	rps4-trnT	1	AGTCCAGGTAAAGCCCCTAG	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.251799	46245	46709	584-604	1.000000	83.2
rps4-trnT_p2	rps4-trnT	2	GTCCAGGTAAAGCCCCTAGA	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.257252	46245	46709	583-603	1.000000	83.2
rps4-trnT_p3	rps4-trnT	3	TCCAGGTAAAGCCCCTAGAC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.257252	46245	46709	582-602	1.000000	83.2
rps4-trnT_p4	rps4-trnT	4	AGTCCAGGTAAAGCCCCTAG	GGTTCGATTCCGATAGCTGG	20	20	55.0	55.0	58.1	58.2	0.0	0.0	0.334534	46245	46709	543-563	1.000000	83.0
rps4-trnT_p5	rps4-trnT	5	TCCAGGTAAAGCCCCTAGAC	GGTTCGATTCCGATAGCTGG	20	20	55.0	55.0	58.1	58.2	0.0	0.0	0.339987	46245	46709	541-561	1.000000	83.0
trnT-trnL-UAA_p1	trnT-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	AGCCAATTAAGTCCGTAGCG	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.196808	46783	47435	709-736	1.000000	85.3
trnT-trnL-UAA_p2	trnT-trnL-UAA	2	AAAGCCAGCTATCGGAATCG	AGCCAATTAAGTCCGTAGCG	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.192987	46783	47435	754-781	1.000000	84.9
trnT-trnL-UAA_p3	trnT-trnL-UAA	3	GGAATCGAACCGATGACCAT	AGCCAATTAAGTCCGTAGCG	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.304561	46783	47435	741-768	1.000000	84.1
trnT-trnL-UAA_p4	trnT-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46783	47435	697-724	1.000000	83.9
trnT-trnL-UAA_p5	trnT-trnL-UAA	5	AAAGCCAGCTATCGGAATCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.1	58.2	0.0	10.2	0.377368	46783	47435	742-769	1.000000	83.5
accD-psaI_p1	accD-psaI	1	TTGATCCAATCGTACCACGT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.0	58.0	0.0	26.9	1.069577	59002	59522	655-696	1.000000	79.1
accD-psaI_p2	accD-psaI	2	TTTGATCCAATCGTACCACGT	CCATTGCAATTGCCGGAAAT	21	20	42.9	45.0	57.7	58.0	0.0	26.9	1.349215	59002	59522	656-697	1.000000	76.9
accD-psaI_p3	accD-psaI	3	TTGATCCAATCGTACCACGT	GCCGGAAATACTAGGCCTAC	20	20	45.0	55.0	57.0	57.5	0.0	8.9	1.494869	59002	59522	644-685	1.000000	75.8
accD-psaI_p4	accD-psaI	4	TTGATCCAATCGTACCACGT	AAAGACGCCATTGCAATTGC	20	20	45.0	45.0	57.0	58.6	0.0	23.7	1.611078	59002	59522	662-703	1.000000	74.7
accD-psaI_p5	accD-psaI	5	TTTGATCCAATCGTACCACGT	GCCGGAAATACTAGGCCTAC	21	20	42.9	55.0	57.7	57.5	0.0	8.9	1.774507	59002	59522	645-686	1.000000	73.5
petA-psbJ_p1	petA-psbJ	1	TGCGCGTACAAGGTCTTTT	CTGGATTAGGCTCATCGCTG	19	20	47.4	55.0	58.0	58.2	8.4	2.8	1.211752	62750	63676	1057-1070	1.000000	73.3
petA-psbJ_p2	petA-psbJ	2	CGCGTACAAGGTCTTTTGTT	CTGGATTAGGCTCATCGCTG	20	20	45.0	55.0	57.0	58.2	0.0	2.8	1.237383	62750	63676	1055-1068	1.000000	73.1
petA-psbJ_p3	petA-psbJ	3	TGCGCGTACAAGGTCTTTT	TCTGGATTAGGCTCATCGCT	19	20	47.4	50.0	58.0	58.3	8.4	2.8	1.295432	62750	63676	1058-1071	1.000000	72.6
petA-psbJ_p4	petA-psbJ	4	CGCGTACAAGGTCTTTTGTTC	CTGGATTAGGCTCATCGCTG	21	20	47.6	55.0	58.1	58.2	0.0	2.8	1.306765	62750	63676	1055-1068	1.000000	72.6
petA-psbJ_p5	petA-psbJ	5	CGCGTACAAGGTCTTTTGTT	TCTGGATTAGGCTCATCGCT	20	20	45.0	50.0	57.0	58.3	0.0	2.8	1.321063	62750	63676	1056-1069	1.000000	72.4
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AGCGGAGGTTATAGTTAAAGCC	20	22	50.0	45.5	57.9	57.9	6.4	0.0	2.120635	64458	65328	1032-1061	1.000000	67.2
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	GGAGGTTATAGTTAAAGCCGCT	20	22	50.0	45.5	57.9	57.9	6.4	0.0	2.120635	64458	65328	1029-1058	1.000000	67.2
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	GGAGGTTATAGTTAAAGCCGCT	20	22	50.0	45.5	57.8	57.9	24.9	0.0	2.225643	64458	65328	952-981	1.000000	66.7
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	AGCGGAGGTTATAGTTAAAGCC	20	22	50.0	45.5	57.8	57.9	24.9	0.0	2.225643	64458	65328	955-984	1.000000	66.6
psbE-petL_p5	psbE-petL	5	ACGAATAACCAACCCGCAAT	GGAGGTTATAGTTAAAGCCGCT	20	22	45.0	45.5	58.2	57.9	0.0	0.0	2.240321	64458	65328	1038-1067	1.000000	66.2
psaJ-rpl33_p1	psaJ-rpl33	1	TTTTCCCAGATGCGTTGACA	ATATCTGGAAACGCCCCTTG	20	20	45.0	50.0	58.0	57.7	0.0	0.0	0.374146	66726	67067	474-478	1.000000	83.1
psaJ-rpl33_p2	psaJ-rpl33	2	ATGCGTTGACATTCCCCTTT	ATATCTGGAAACGCCCCTTG	20	20	45.0	50.0	58.1	57.7	0.0	0.0	0.425055	66726	67067	465-469	1.000000	82.8
psaJ-rpl33_p3	psaJ-rpl33	3	TTGGGTCTTTAGCCGGTCTA	ATATCTGGAAACGCCCCTTG	20	20	50.0	50.0	58.1	57.7	1.9	0.0	0.407034	66726	67067	513-517	1.000000	82.3
psaJ-rpl33_p4	psaJ-rpl33	4	ACATATCTTTCCGTGGCACC	ATATCTGGAAACGCCCCTTG	20	20	50.0	50.0	58.0	57.7	12.9	0.0	0.386798	66726	67067	553-557	1.000000	82.1
psaJ-rpl33_p5	psaJ-rpl33	5	ATGGTTTGGGTCTTTAGCCG	ATATCTGGAAACGCCCCTTG	20	20	50.0	50.0	58.2	57.7	0.0	0.0	0.514223	66726	67067	518-522	1.000000	81.4
rpl33-rps18_p1	rpl33-rps18	1	CTAGTCGGTTGGAATTGCGA	CAAACGCCTACGAAAAGCTC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.232336	67269	67553	398-432	1.000000	84.6
rpl33-rps18_p2	rpl33-rps18	2	CTAGTCGGTTGGAATTGCGA	TATTCGATCCCCCGATTGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.022938	67269	67553	425-459	0.950000	84.1
rpl33-rps18_p3	rpl33-rps18	3	GACACAATACGCCTAGTCGG	TATTCGATCCCCCGATTGGA	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.181972	67269	67553	437-471	0.950000	82.8
rpl33-rps18_p4	rpl33-rps18	4	ACAATACGCCTAGTCGGTTG	TATTCGATCCCCCGATTGGA	20	20	50.0	50.0	57.7	58.0	10.6	0.0	0.307316	67269	67553	434-468	0.950000	81.8
rpl33-rps18_p5	rpl33-rps18	5	CACAATACGCCTAGTCGGTT	TATTCGATCCCCCGATTGGA	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.307316	67269	67553	435-469	0.950000	81.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGCAGCGTTAAAAGCCTTTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.9	58.0	7.7	0.0	0.156746	111689	112867	1111-1305	1.000000	89.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGCAGCGTTAAAAGCCTTTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.9	58.0	7.7	0.0	0.158116	111689	112867	1112-1306	1.000000	89.6
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGCAGCGTTAAAAGCCTTTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.9	57.9	7.7	0.0	0.273193	111689	112867	1121-1315	1.000000	88.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGCAGCGTTAAAAGCCTTTT	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	57.9	57.8	7.7	0.0	0.345211	111689	112867	1120-1314	1.000000	88.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGCAGCGTTAAAAGCCTTTT	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	57.9	57.8	7.7	0.0	0.345211	111689	112867	1119-1313	1.000000	88.1
ycf1_p1	ycf1	1	TTACGTCTTTGAGCACGCAT	AAGTTCCTCGTTGGTCACAC	20	20	45.0	50.0	58.2	58.1	0.0	0.0	0.259780	125846	125965	235-259	1.000000	89.9
ycf1_p2	ycf1	2	TCAGATTGTTGGGCATACCG	AAGTTCCTCGTTGGTCACAC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.087492	125846	125965	211-211	0.950000	83.4
ycf1_p3	ycf1	3	TACCGTGTCAAAGCCACTTC	AAGTTCCTCGTTGGTCACAC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.176837	125846	125965	196-196	0.950000	82.7
ycf1_p4	ycf1	4	CATACCGTGTCAAAGCCACT	AAGTTCCTCGTTGGTCACAC	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.242124	125846	125965	198-198	0.950000	82.1
ycf1_p5	ycf1	5	AGATTGTTGGGCATACCGTG	AAGTTCCTCGTTGGTCACAC	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.300342	125846	125965	209-209	0.950000	81.7