primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	trnH-CAC-psbA	1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	20	20	50.0	55.0	57.9	58.1	0.0	12.9	0.188044	76	452	402-435	1.000000	90.5
trnH-CAC-psbA_p2	trnH-CAC-psbA	2	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	76	452	417-450	1.000000	90.4
trnH-CAC-psbA_p3	trnH-CAC-psbA	3	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	76	452	420-453	1.000000	90.3
trnH-CAC-psbA_p4	trnH-CAC-psbA	4	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	20	20	50.0	55.0	58.2	58.1	0.0	12.9	0.213831	76	452	403-436	1.000000	90.3
trnH-CAC-psbA_p5	trnH-CAC-psbA	5	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	58.2	57.9	0.0	13.4	0.226475	76	452	418-451	1.000000	90.2
trnK-AAA-rps16_p1	trnK-AAA-rps16	1	CCTTTCAGGATCAGTCGTGG	GCCTTGTTGCTGTCGTTTTT	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.094492	4403	5123	1358-1424	1.000000	90.0
trnK-AAA-rps16_p2	trnK-AAA-rps16	2	TCGACACCGAAAACAAGGTT	GCCTTGTTGCTGTCGTTTTT	20	20	45.0	45.0	58.0	58.1	0.0	0.0	0.105760	4403	5123	1450-1516	1.000000	89.8
trnK-AAA-rps16_p3	trnK-AAA-rps16	3	TGGTCTTACAAACGCTACCG	GCCTTGTTGCTGTCGTTTTT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.147593	4403	5123	1341-1407	1.000000	89.6
trnK-AAA-rps16_p4	trnK-AAA-rps16	4	TCAGGATCAGTCGTGGTCTT	GCCTTGTTGCTGTCGTTTTT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.154025	4403	5123	1354-1420	1.000000	89.4
trnK-AAA-rps16_p5	trnK-AAA-rps16	5	TTCAGGATCAGTCGTGGTCT	GCCTTGTTGCTGTCGTTTTT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.154025	4403	5123	1355-1421	1.000000	89.4
rps16-trnQ-CAA_p1	rps16-trnQ-CAA	1	GCAAGCTCTATACGCGTCTT	CAAAACCCTAGCACCACTGT	20	20	50.0	50.0	58.2	58.0	18.6	0.0	0.177294	5309	5988	1116-1116	0.250000	48.6
rps16-trnQ-CAA_p2	rps16-trnQ-CAA	2	GCAAGCTCTATACGCGTCTT	TGGCGACTAAAAAGGTGCTT	20	20	50.0	45.0	58.2	58.0	18.6	0.0	0.182891	5309	5988	1469-1469	0.250000	48.5
rps16-trnQ-CAA_p3	rps16-trnQ-CAA	3	GCAAGCTCTATACGCGTCTT	TGTCAAAACCCTAGCACCAC	20	20	50.0	50.0	58.2	58.0	18.6	0.0	0.182891	5309	5988	1119-1119	0.250000	48.5
rps16-trnQ-CAA_p4	rps16-trnQ-CAA	4	GCAAGCTCTATACGCGTCTT	TTGGCGACTAAAAAGGTGCT	20	20	50.0	45.0	58.2	58.0	18.6	0.0	0.182891	5309	5988	1470-1470	0.250000	48.5
rps16-trnQ-CAA_p5	rps16-trnQ-CAA	5	GCGCCCTTTTCAGTAAATCG	CAAAACCCTAGCACCACTGT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.192804	5309	5988	986-986	0.250000	48.5
trnS-AGC-trnG-GGA_p1	trnS-AGC-trnG-GGA	1	CCAACGCTTTAGTCCACTCA	GGAACGAATCACACTTTTACCA	20	22	50.0	40.9	57.8	56.9	0.0	0.0	3.248264	7373	8110	661-807	1.000000	64.5
trnS-AGC-trnG-GGA_p2	trnS-AGC-trnG-GGA	2	AACGCTTTAGTCCACTCAGC	GGAACGAATCACACTTTTACCA	20	22	50.0	40.9	58.2	56.9	2.0	0.0	3.287421	7373	8110	659-805	1.000000	64.2
trnS-AGC-trnG-GGA_p3	trnS-AGC-trnG-GGA	3	CCAACGCTTTAGTCCACTCA	GGAACGAATCACACTTTTACCAC	20	23	50.0	43.5	57.8	58.2	0.0	0.0	3.383831	7373	8110	661-807	1.000000	63.4
trnS-AGC-trnG-GGA_p4	trnS-AGC-trnG-GGA	4	AACGCTTTAGTCCACTCAGC	GGAACGAATCACACTTTTACCAC	20	23	50.0	43.5	58.2	58.2	2.0	0.0	3.422988	7373	8110	659-805	1.000000	63.1
trnS-AGC-trnG-GGA_p5	trnS-AGC-trnG-GGA	5	CCAACGCTTTAGTCCACTCA	AGGAACGAATCACACTTTTACCA	20	23	50.0	39.1	57.8	58.3	0.0	0.0	3.453494	7373	8110	662-808	1.000000	62.9
trnG-GGA-trnR-AGA_p1	trnG-GGA-trnR-AGA	1	AGCCTTCCAAGCTAACGATG	TGGTATTGCGTCCAATAGGA	20	20	50.0	45.0	58.0	56.3	0.0	0.0	1.708175	8893	9068	274-303	1.000000	74.0
trnG-GGA-trnR-AGA_p2	trnG-GGA-trnR-AGA	2	ACCCTTAGCCTTCCAAGCTA	TGGTATTGCGTCCAATAGGA	20	20	50.0	45.0	58.0	56.3	0.0	0.0	1.711492	8893	9068	280-309	1.000000	73.9
trnG-GGA-trnR-AGA_p3	trnG-GGA-trnR-AGA	3	CGACGTCGACTAAAACCCTT	TGGTATTGCGTCCAATAGGA	20	20	50.0	45.0	57.9	56.3	33.4	0.0	1.734431	8893	9068	294-323	1.000000	73.5
trnG-GGA-trnR-AGA_p4	trnG-GGA-trnR-AGA	4	AAACCCTTAGCCTTCCAAGC	TGGTATTGCGTCCAATAGGA	20	20	50.0	45.0	58.1	56.3	0.0	0.0	1.753606	8893	9068	282-311	1.000000	73.5
trnG-GGA-trnR-AGA_p5	trnG-GGA-trnR-AGA	5	AGCCTTCCAAGCTAACGATG	TTGGTATTGCGTCCAATAGGA	20	21	50.0	42.9	58.0	57.1	0.0	0.0	1.946197	8893	9068	275-304	1.000000	72.0
trnR-AGA-atpA_p1	trnR-AGA-atpA	1	AGCCTTCCAAGCTAACGATG	GGGTCGTGAAGCTTATCCAG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.088513	9141	9377	1208-1304	1.000000	86.0
trnR-AGA-atpA_p2	trnR-AGA-atpA	2	AGCCTTCCAAGCTAACGATG	GGTCGTGAAGCTTATCCAGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.088513	9141	9377	1207-1303	1.000000	86.0
trnR-AGA-atpA_p3	trnR-AGA-atpA	3	CGAAAGGCGTCCATTGTCTA	GGGTCGTGAAGCTTATCCAG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.063258	9141	9377	994-1114	1.000000	84.7
trnR-AGA-atpA_p4	trnR-AGA-atpA	4	CGAAAGGCGTCCATTGTCTA	GGTCGTGAAGCTTATCCAGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.063258	9141	9377	993-1113	1.000000	84.7
trnR-AGA-atpA_p5	trnR-AGA-atpA	5	TTCTGTCAAAGCACCCAGAC	GGTCGTGAAGCTTATCCAGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.091356	9141	9377	1400-1446	0.500000	60.8
atpH-atpI_p1	atpH-atpI	1	AACCGAAGCAGCAGAAATCA	TACAAGCGGGATTCAAGCTC	20	20	45.0	50.0	58.1	58.0	0.0	5.7	0.127956	12979	13451	582-601	1.000000	84.8
atpH-atpI_p2	atpH-atpI	2	GTCCAATAGAAGCAAGCCCA	TACAAGCGGGATTCAAGCTC	20	20	50.0	50.0	57.9	58.0	0.0	5.7	0.152946	12979	13451	622-641	1.000000	84.2
atpH-atpI_p3	atpH-atpI	3	AACCGAAGCAGCAGAAATCA	TTTTTGCCACTTTAGCTGCG	20	20	45.0	45.0	58.1	57.9	0.0	0.0	0.236520	12979	13451	559-578	1.000000	84.2
atpH-atpI_p4	atpH-atpI	4	GTCCAATAGAAGCAAGCCCA	TTTTTGCCACTTTAGCTGCG	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.261511	12979	13451	599-618	1.000000	83.6
atpH-atpI_p5	atpH-atpI	5	AGGTCCAATAGAAGCAAGCC	TACAAGCGGGATTCAAGCTC	20	20	50.0	50.0	57.6	58.0	0.0	5.7	0.451018	12979	13451	624-643	1.000000	81.8
psbM-trnD-GAC_p1	psbM-trnD-GAC	1	AGGAACGAGAATAAAGAGTGCA	TTCAATTGGTCAGAGCACCG	22	20	40.9	50.0	57.5	58.5	0.0	5.4	3.014466	27838	28876	1133-1157	1.000000	64.9
psbM-trnD-GAC_p2	psbM-trnD-GAC	2	GGAACGAGAATAAAGAGTGCAG	TTCAATTGGTCAGAGCACCG	22	20	45.5	50.0	57.2	58.5	0.0	5.4	3.326442	27838	28876	1132-1156	1.000000	62.4
psbM-trnD-GAC_p3	psbM-trnD-GAC	3	GGAACGAGAATAAAGAGTGCA	TTCAATTGGTCAGAGCACCG	21	20	42.9	50.0	56.0	58.5	0.0	5.4	3.473883	27838	28876	1132-1156	1.000000	61.2
psbM-trnD-GAC_p4	psbM-trnD-GAC	4	CGAGAATAAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	22	20	45.5	50.0	57.0	58.5	0.0	5.4	3.503685	27838	28876	1128-1152	1.000000	61.0
psbM-trnD-GAC_p5	psbM-trnD-GAC	5	AGGAACGAGAATAAAGAGTGCA	GTTCAATTGGTCAGAGCACC	22	20	40.9	50.0	57.5	57.0	0.0	5.4	3.534620	27838	28876	1134-1158	1.000000	60.7
trnT-ACA-trnL-UUA_p1	trnT-ACA-trnL-UUA	1	GCGATGCTCTAACCTCTGAG	ATTAAGTCCGCAGCGTCTAC	20	20	55.0	50.0	57.9	57.8	0.0	15.0	0.338047	45073	45851	806-911	1.000000	87.0
trnT-ACA-trnL-UUA_p2	trnT-ACA-trnL-UUA	2	GGAATCGAACCGATGACCAT	ATTAAGTCCGCAGCGTCTAC	20	20	50.0	50.0	57.8	57.8	15.5	15.0	0.445800	45073	45851	838-943	1.000000	85.9
trnT-ACA-trnL-UUA_p3	trnT-ACA-trnL-UUA	3	GCGATGCTCTAACCTCTGAG	CGTCTACCAATTCCGCCATA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.652117	45073	45851	793-898	1.000000	84.6
trnT-ACA-trnL-UUA_p4	trnT-ACA-trnL-UUA	4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	45073	45851	831-936	1.000000	84.4
trnT-ACA-trnL-UUA_p5	trnT-ACA-trnL-UUA	5	CTAACCTCTGAGCTAAGCGG	ATTAAGTCCGCAGCGTCTAC	20	20	55.0	50.0	57.5	57.8	5.5	15.0	0.705816	45073	45851	798-903	1.000000	84.1
ndhC-trnV-GUA_p1	ndhC-trnV-GUA	1	GCCCAGAAAATATCATATTCGT	GGTTCGAATCCGTATAGCCC	22	20	36.4	55.0	54.4	57.9	0.0	0.0	5.684147	48687	49422	793-872	1.000000	48.0
ndhC-trnV-GUA_p2	ndhC-trnV-GUA	2	GCCCAGAAAATATCATATTCGT	CGGTTCGAATCCGTATAGCC	22	20	36.4	55.0	54.4	58.3	0.0	22.8	5.913528	48687	49422	794-873	1.000000	48.0
ndhC-trnV-GUA_p3	ndhC-trnV-GUA	3	GCCCAGAAAATATCATATTCGT	CAGGTCTACGGTTCGAATCC	22	20	36.4	55.0	54.4	57.8	0.0	0.0	5.813474	48687	49422	802-881	1.000000	47.9
ndhC-trnV-GUA_p4	ndhC-trnV-GUA	4	GCCCAGAAAATATCATATTCGT	GCAGGTCTACGGTTCGAATC	22	20	36.4	55.0	54.4	58.4	0.0	0.0	6.050516	48687	49422	803-882	1.000000	47.9
ndhC-trnV-GUA_p5	ndhC-trnV-GUA	5	GCCCAGAAAATATCATATTCGT	AGCAGGTCTACGGTTCGAAT	22	20	36.4	50.0	54.4	58.8	0.0	0.0	6.424619	48687	49422	804-883	1.000000	47.9
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	CTGGACGGATTCCTCTTTGG	20	20	50.0	55.0	58.0	58.0	0.0	0.3	0.068225	60646	61608	1098-1203	1.000000	86.9
petA-psbJ_p2	petA-psbJ	2	CATTACGTGTCCAAGGCCTT	CTGGACGGATTCCTCTTTGG	20	20	50.0	55.0	58.2	58.0	13.8	0.3	0.208622	60646	61608	1095-1200	1.000000	85.8
petA-psbJ_p3	petA-psbJ	3	TTACGTGTCCAAGGCCTTTT	CTGGACGGATTCCTCTTTGG	20	20	45.0	55.0	57.6	58.0	17.6	0.3	0.381544	60646	61608	1093-1198	1.000000	84.4
petA-psbJ_p4	petA-psbJ	4	TACGTGTCCAAGGCCTTTTG	CTGGACGGATTCCTCTTTGG	20	20	50.0	55.0	58.4	58.0	17.6	0.3	0.429815	60646	61608	1092-1197	1.000000	84.1
petA-psbJ_p5	petA-psbJ	5	TGTCCAAGGCCTTTTGTTCT	CTGGACGGATTCCTCTTTGG	20	20	45.0	55.0	57.5	58.0	17.6	0.3	0.482439	60646	61608	1088-1193	1.000000	83.7
trnW-UGG-trnP-CCA_p1	trnW-UGG-trnP-CCA	1	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.144618	63842	64030	250-258	1.000000	87.0
trnW-UGG-trnP-CCA_p2	trnW-UGG-trnP-CCA	2	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145952	63842	64030	248-256	1.000000	87.0
trnW-UGG-trnP-CCA_p3	trnW-UGG-trnP-CCA	3	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145952	63842	64030	251-259	1.000000	86.9
trnW-UGG-trnP-CCA_p4	trnW-UGG-trnP-CCA	4	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.136582	63842	64030	261-269	1.000000	86.8
trnW-UGG-trnP-CCA_p5	trnW-UGG-trnP-CCA	5	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.137926	63842	64030	262-270	1.000000	86.8
psaJ-rpl33_p1	psaJ-rpl33	1	ACCCGTGACATTTTGTACCC	GGGGTGTTATGCCGATTCTT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.152537	64585	65011	1036-1101	1.000000	86.3
psaJ-rpl33_p2	psaJ-rpl33	2	CCCGTGACATTTTGTACCCA	GGGGTGTTATGCCGATTCTT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.153880	64585	65011	1035-1100	1.000000	86.3
psaJ-rpl33_p3	psaJ-rpl33	3	ACCCGTGACATTTTGTACCC	TCCAATCGACTAGGGGTGTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.152770	64585	65011	1048-1113	1.000000	86.2
psaJ-rpl33_p4	psaJ-rpl33	4	CCCGTGACATTTTGTACCCA	TCCAATCGACTAGGGGTGTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.154114	64585	65011	1047-1112	1.000000	86.2
psaJ-rpl33_p5	psaJ-rpl33	5	ACCCGTGACATTTTGTACCC	TTCCAATCGACTAGGGGTGT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.152770	64585	65011	1103-1114	0.500000	60.4
rpl16_p1	rpl16	1	ATCGGCGATTACTAGCTCCT	GTATGGAGGGAATTGCACGT	20	20	50.0	50.0	58.1	58.0	18.1	0.0	0.125970	78573	79975	2223-2313	1.000000	86.8
rpl16_p2	rpl16	2	TAACGTCACCAATATGCGCA	TGAACAGGCGAATACAAGGG	20	20	45.0	50.0	58.0	57.9	15.0	0.0	0.119490	78573	79975	1955-2045	0.750000	76.8
rpl16_p3	rpl16	3	TAACGTCACCAATATGCGCA	GTATGGAGGGAATTGCACGT	20	20	45.0	50.0	58.0	58.0	15.0	0.0	0.049927	78573	79975	2244-2334	0.750000	76.7
rpl16_p4	rpl16	4	ACACATCAATTCTCGAGCCC	GTATGGAGGGAATTGCACGT	20	20	50.0	50.0	58.0	58.0	7.8	0.0	0.068225	78573	79975	2192-2282	0.750000	76.7
rpl16_p5	rpl16	5	TAACGTCACCAATATGCGCA	TGCACGTGTTGAATGGATCA	20	20	45.0	45.0	58.0	58.1	15.0	18.4	0.121995	78573	79975	1982-1982	0.250000	49.0
ndhF-rpl32_p1	ndhF-rpl32	1	TCCATGAATATTGATATGTATGTTCCA	ACGAATACGCTTTTTCGAGA	27	20	29.6	40.0	56.7	55.5	0.0	19.5	10.706462	109922	110631	381-786	1.000000	50.3
ndhF-rpl32_p2	ndhF-rpl32	2	CCATGAATATTGATATGTATGTTCCA	ACGAATACGCTTTTTCGAGA	26	20	30.8	40.0	55.5	55.5	0.0	19.5	10.945020	109922	110631	380-785	1.000000	50.3
ndhF-rpl32_p3	ndhF-rpl32	3	TCCATGAATATTGATATGTATGTTCCA	ACGAATACGCTTTTTCGAGAT	27	21	29.6	38.1	56.7	56.1	0.0	19.5	11.150403	109922	110631	381-786	1.000000	50.3
ndhF-rpl32_p4	ndhF-rpl32	4	TCCATGAATATTGATATGTATGTTCC	ACGAATACGCTTTTTCGAGA	26	20	30.8	40.0	55.3	55.5	0.0	19.5	11.174465	109922	110631	381-786	1.000000	50.3
ndhF-rpl32_p5	ndhF-rpl32	5	CCATGAATATTGATATGTATGTTCCA	ACGAATACGCTTTTTCGAGAT	26	21	30.8	38.1	55.5	56.1	0.0	19.5	11.388961	109922	110631	380-785	1.000000	50.3
ndhE-ndhG_p1	ndhE-ndhG	1	TTCGACTCGTGATCAATCCA	TTTCGGTGGCTCGTCAATAA	20	20	45.0	45.0	56.7	57.5	0.0	1.4	1.775971	115446	115719	333-381	1.000000	74.0
ndhE-ndhG_p2	ndhE-ndhG	2	TTCGACTCGTGATCAATCCA	ATTTCGGTGGCTCGTCAATA	20	20	45.0	45.0	56.7	57.3	0.0	1.4	2.000912	115446	115719	334-382	1.000000	72.2
ndhE-ndhG_p3	ndhE-ndhG	3	TCGACTCGTGATCAATCCAT	TTTCGGTGGCTCGTCAATAA	20	20	45.0	45.0	56.5	57.5	0.0	1.4	2.004375	115446	115719	332-380	1.000000	72.2
ndhE-ndhG_p4	ndhE-ndhG	4	TTTCGACTCGTGATCAATCCA	TTTCGGTGGCTCGTCAATAA	21	20	42.9	45.0	57.4	57.5	0.0	1.4	2.045161	115446	115719	334-382	1.000000	71.8
ndhE-ndhG_p5	ndhE-ndhG	5	TCGACTCGTGATCAATCCAT	ATTTCGGTGGCTCGTCAATA	20	20	45.0	45.0	56.5	57.3	0.0	1.4	2.229316	115446	115719	333-381	1.000000	70.4
ycf1_p1	ycf1	1	GTTTTACGCCTTTGGGAACG	GGAAGAATTGGAAGCCGACT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.156057	124439	124558	240-240	0.500000	61.4
ycf1_p2	ycf1	2	GTTTTACGCCTTTGGGAACG	TGGAAGAATTGGAAGCCGAC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.219498	124439	124558	241-241	0.500000	60.9
ycf1_p3	ycf1	3	TCGGATAGTTGAACCCCAGT	GGAAGAATTGGAAGCCGACT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.168358	124439	124558	663-663	0.500000	60.7
ycf1_p4	ycf1	4	GTTTTACGCCTTTGGGAACG	GTCCCAATGGAACCCGAAAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.193386	124439	124558	209-209	0.500000	60.4
ycf1_p5	ycf1	5	TCGGATAGTTGAACCCCAGT	GTCCCAATGGAACCCGAAAT	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.205687	124439	124558	632-632	0.500000	60.1