primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	58.0	55.5	0.0	0.0	3.574049	8423	8943	566-601	1.000000	63.4
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	20	22	45.0	36.4	58.0	56.2	0.0	0.0	3.814520	8423	8943	567-602	1.000000	61.5
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	GCTTTAGTCCACTCAGCCAT	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.6	55.5	0.9	0.0	3.945791	8423	8943	552-587	1.000000	60.4
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	ATTAGCAATCCGCCGCTTTA	TACCACTAAACTATACCCGC	20	20	45.0	45.0	58.0	54.0	0.0	0.0	4.049283	8423	8943	552-587	1.000000	59.6
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AGTCCACTCAGCCATCTCTC	ACGAATCACACTTTTACCACT	20	21	55.0	38.1	58.5	55.5	0.0	0.0	4.051804	8423	8943	547-582	1.000000	59.6
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TGCTTTGGCTTTACTCACGT	20	20	45.0	45.0	58.0	58.0	1.4	0.0	0.076593	13450	14501	1415-1467	1.000000	88.7
atpH-atpI_p2	atpH-atpI	2	ATAAACAACCCGCACACACT	TGCTTTGGCTTTACTCACGT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.062547	13450	14501	2122-2168	1.000000	88.6
atpH-atpI_p3	atpH-atpI	3	ATAAACAACCCGCACACACT	GAATACGGTCCTTGGGTTCC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.058445	13450	14501	2268-2314	1.000000	88.2
atpH-atpI_p4	atpH-atpI	4	ATAACGGAAGCGGCAGAAAT	GAATACGGTCCTTGGGTTCC	20	20	45.0	55.0	58.0	58.0	1.4	0.0	0.072491	13450	14501	1561-1613	1.000000	88.1
atpH-atpI_p5	atpH-atpI	5	ATAAACAACCCGCACACACT	TTCGGAACCCACAAACCATT	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.099069	13450	14501	2363-2409	1.000000	87.6
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CTCATTTCCATCCCCGAGC	CTTACCACTCGGCCATGC	19	18	57.9	61.1	58.0	58.2	0.0	2.2	3.198964	26722	28002	1277-1324	1.000000	66.4
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	ATTCCCTCATTTCCATCCCC	CTTACCACTCGGCCATGC	20	18	50.0	61.1	56.9	58.2	0.0	2.2	3.267460	26722	28002	1282-1329	1.000000	65.9
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCATTTCCATCCCCGAGCAT	CTTACCACTCGGCCATGC	20	18	50.0	61.1	59.2	58.2	0.0	2.2	3.340389	26722	28002	1276-1323	1.000000	65.3
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	ACATTCCCTCATTTCCATCCC	CTTACCACTCGGCCATGC	21	18	47.6	61.1	57.7	58.2	0.0	2.2	3.505435	26722	28002	1284-1331	1.000000	64.0
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CTCATTTCCATCCCCGAGC	CCTTACCACTCGGCCATG	19	18	57.9	61.1	58.0	57.5	0.0	2.2	3.552713	26722	28002	1278-1325	1.000000	63.6
trnC-GCA-petN_p1	trnC-GCA-petN	1	CGCAGTCAGCAGTCAATAGT	ACTTCTTCCCCACACTACGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.007182	28074	28948	1295-1317	1.000000	91.9
trnC-GCA-petN_p2	trnC-GCA-petN	2	CGCAGTCAGCAGTCAATAGT	TTCTTCCCCACACTACGAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.007182	28074	28948	1302-1315	0.666667	76.5
trnC-GCA-petN_p3	trnC-GCA-petN	3	ATACTAGACGGGGATGGGTG	ACTTCTTCCCCACACTACGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.016493	28074	28948	1203-1203	0.333333	53.2
trnC-GCA-petN_p4	trnC-GCA-petN	4	ATACTAGACGGGGATGGGTG	TTCTTCCCCACACTACGAGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.016493	28074	28948	1201-1201	0.333333	53.2
trnC-GCA-petN_p5	trnC-GCA-petN	5	GGATGGGTGTGGATCCAATC	TTCTTCCCCACACTACGAGT	20	20	55.0	50.0	58.0	58.0	18.2	0.0	0.018488	28074	28948	1190-1190	0.333333	53.2
petN-psbM_p1	petN-psbM	1	TAGGTTGGAGAGCCCGATAG	TTCAAAACCAGCGGGATTCA	20	20	55.0	45.0	58.0	58.0	0.0	10.9	0.014908	29039	30100	1982-1982	0.333333	53.2
petN-psbM_p2	petN-psbM	2	ACTCGTAGTGTGGGGAAGAA	TTCAAAACCAGCGGGATTCA	20	20	50.0	45.0	58.0	58.0	0.0	10.9	0.017077	29039	30100	1312-1312	0.333333	53.2
petN-psbM_p3	petN-psbM	3	TCGTAGTGTGGGGAAGAAGT	TTCAAAACCAGCGGGATTCA	20	20	50.0	45.0	58.0	58.0	0.0	10.9	0.017077	29039	30100	1310-1310	0.333333	53.2
petN-psbM_p4	petN-psbM	4	AGTGTCTAATGGCTGGACCT	TTCAAAACCAGCGGGATTCA	20	20	50.0	45.0	58.0	58.0	0.0	10.9	0.053418	29039	30100	2007-2007	0.333333	52.9
petN-psbM_p5	petN-psbM	5	GAATTAGGTTGGAGAGCCCG	TTCAAAACCAGCGGGATTCA	20	20	55.0	45.0	58.0	58.0	0.0	10.9	0.056780	29039	30100	1986-1986	0.333333	52.9
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AAATGCAAGAATATTTACTTCCA	TTCAATTGGTCAGAGCACCG	23	20	26.1	50.0	52.7	58.5	0.0	5.4	8.816568	30206	31043	926-946	1.000000	51.3
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AATGCAAGAATATTTACTTCCAT	TTCAATTGGTCAGAGCACCG	23	20	26.1	50.0	52.4	58.5	0.0	5.4	9.034411	30206	31043	925-945	1.000000	51.3
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AAATGCAAGAATATTTACTTCCAT	TTCAATTGGTCAGAGCACCG	24	20	25.0	50.0	53.3	58.5	0.0	5.4	9.197929	30206	31043	926-946	1.000000	51.3
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AAATGCAAGAATATTTACTTCCA	GTTCAATTGGTCAGAGCACC	23	20	26.1	50.0	52.7	57.0	0.0	5.4	9.336721	30206	31043	927-947	1.000000	51.3
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AATGCAAGAATATTTACTTCCAT	GTTCAATTGGTCAGAGCACC	23	20	26.1	50.0	52.4	57.0	0.0	5.4	9.554565	30206	31043	926-946	1.000000	51.3
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CCATTTATTCGCGATCTAGGC	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	57.0	58.1	10.9	0.0	2.093591	45258	45755	560-604	1.000000	73.4
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CCATTTATTCGCGATCTAGGC	TTCCAATGCTACGCCTTGAA	21	20	47.6	45.0	57.0	57.8	10.9	5.6	2.191892	45258	45755	531-575	1.000000	73.0
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CCATTTATTCGCGATCTAGGC	ACCCTCGGTAAACAAAAGCC	21	20	47.6	50.0	57.0	58.4	10.9	0.0	2.386552	45258	45755	561-605	1.000000	71.1
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TCCATTTATTCGCGATCTAGGC	CCCTCGGTAAACAAAAGCCT	22	20	45.5	50.0	58.4	58.1	10.9	0.0	2.508500	45258	45755	561-605	1.000000	70.1
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	CCATTTATTCGCGATCTAGGC	AGTTCCAATGCTACGCCTTG	21	20	47.6	50.0	57.0	58.5	10.9	0.0	2.546074	45258	45755	533-577	1.000000	70.1
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	47184	47950	828-884	1.000000	89.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	33.1	10.2	0.482748	47184	47950	879-935	1.000000	88.1
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	47184	47950	860-916	1.000000	88.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	47184	47950	822-878	1.000000	87.7
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	47184	47950	860-916	1.000000	86.9
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GTACCATAAAGAAGCGGCCA	GAGGTGCCTATTTAGGTGCC	20	20	50.0	55.0	58.0	58.0	2.2	5.6	0.063976	51303	51977	1702-2434	1.000000	88.0
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGGTACCATAAAGAAGCGGC	GAGGTGCCTATTTAGGTGCC	20	20	50.0	55.0	58.0	58.0	11.6	5.6	0.063976	51303	51977	1704-2436	1.000000	88.0
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCAAAGTCGAATCGTGACCC	GAGGTGCCTATTTAGGTGCC	20	20	50.0	55.0	57.9	58.0	1.5	5.6	0.105843	51303	51977	1756-2488	1.000000	87.5
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GTACCATAAAGAAGCGGCCA	GGTGCCAAATAGACCCTACG	20	20	50.0	55.0	58.0	58.1	2.2	0.0	0.083215	51303	51977	1688-1688	0.333333	52.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGGTACCATAAAGAAGCGGC	GGTGCCAAATAGACCCTACG	20	20	50.0	55.0	58.0	58.1	11.6	0.0	0.083215	51303	51977	1690-1690	0.333333	52.7
rps12-psbB_p1	rps12-psbB	1	GGCTGTCTTGTATTTCTAATAAGTTGT	GGCCGGGATCATTCAATACA	27	20	33.3	50.0	58.1	57.7	0.0	2.2	7.323237	70326	72775	2543-2707	1.000000	52.0
rps12-psbB_p2	rps12-psbB	2	GGCTGTCTTGTATTTCTAATAAGT	GGCCGGGATCATTCAATACA	24	20	33.3	50.0	54.7	57.7	0.0	2.2	7.571508	70326	72775	2543-2707	1.000000	52.0
rps12-psbB_p3	rps12-psbB	3	GGCTGTCTTGTATTTCTAATAAGTTG	GGCCGGGATCATTCAATACA	26	20	34.6	50.0	56.7	57.7	0.0	2.2	7.617248	70326	72775	2543-2707	1.000000	52.0
rps12-psbB_p4	rps12-psbB	4	GGCTGTCTTGTATTTCTAATAAGTT	GGCCGGGATCATTCAATACA	25	20	32.0	50.0	55.4	57.7	0.0	2.2	7.868553	70326	72775	2543-2707	1.000000	52.0
rps12-psbB_p5	rps12-psbB	5	GGCTGTCTTGTATTTCTAATAAGTTGT	CGGCCGGGATCATTCAATAC	27	20	33.3	55.0	58.1	58.8	0.0	20.1	7.896149	70326	72775	2544-2708	1.000000	52.0
ycf15-trnL-CAA_p1	ycf15-trnL-CAA	1	ATACCCGTCACATGCTTTCC	TGAGGAGAGCTTTGTGGAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.053843	94990	95989	1309-1309	0.333333	52.9
ycf15-trnL-CAA_p2	ycf15-trnL-CAA	2	ATACCCGTCACATGCTTTCC	AGAAGGGGTTGGAGTCGTTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.058194	94990	95989	1191-1191	0.333333	52.9
ycf15-trnL-CAA_p3	ycf15-trnL-CAA	3	TCGTTCCAGTGAAAAGGCAA	TGAGGAGAGCTTTGTGGAGA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.061018	94990	95989	1917-1917	0.333333	52.8
ycf15-trnL-CAA_p4	ycf15-trnL-CAA	4	TTCGTTCCAGTGAAAAGGCA	TGAGGAGAGCTTTGTGGAGA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.061018	94990	95989	1918-1918	0.333333	52.8
ycf15-trnL-CAA_p5	ycf15-trnL-CAA	5	ATACCCGTCACATGCTTTCC	CATTCCGATGTAGTGGTGCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.061151	94990	95989	1210-1210	0.333333	52.8
trnN-GUU-rps15_p1	trnN-GUU-rps15	1	AAGAGAAGAAAGGGCGCAAA	TTTGCAGACCTTCCCTTTCC	20	20	45.0	50.0	58.0	58.0	10.8	0.0	0.023367	117975	118574	1217-1217	0.333333	53.1
trnN-GUU-rps15_p2	trnN-GUU-rps15	2	AAGAGAAGAAAGGGCGCAAA	GACCATCGCGGGACTATTTT	20	20	45.0	50.0	58.0	58.1	10.8	6.1	0.069278	117975	118574	1278-1278	0.333333	52.8
trnN-GUU-rps15_p3	trnN-GUU-rps15	3	AAGAGAAGAAAGGGCGCAAA	TCCCTTTCCATTGAGTCCCT	20	20	45.0	50.0	58.0	57.9	10.8	0.0	0.078500	117975	118574	1206-1206	0.333333	52.7
trnN-GUU-rps15_p4	trnN-GUU-rps15	4	AAGAGAAGAAAGGGCGCAAA	TATGACCATCGCGGGACTAT	20	20	45.0	50.0	58.0	58.1	10.8	6.1	0.099503	117975	118574	1281-1281	0.333333	52.5
trnN-GUU-rps15_p5	trnN-GUU-rps15	5	AAGAGAAGAAAGGGCGCAAA	CGAGAGGTCCCCCTTAAGAA	20	20	45.0	55.0	58.0	58.1	10.8	3.4	0.156762	117975	118574	1005-1005	0.333333	52.1
ndhF-rpl32_p1	ndhF-rpl32	1	TGAGCCACCCGTATGGATAT	TTGAACGCTGTCCAATACCC	20	20	50.0	50.0	58.0	58.2	1.3	0.0	0.208101	128733	129380	768-776	0.666667	77.0
ndhF-rpl32_p2	ndhF-rpl32	2	CAAGAGGTCGTGTGAACCAA	TTGAACGCTGTCCAATACCC	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.235821	128733	129380	1248-1256	0.666667	74.6
ndhF-rpl32_p3	ndhF-rpl32	3	ATGAGCCACCCGTATGGATA	TTGAACGCTGTCCAATACCC	20	20	50.0	50.0	58.0	58.2	1.3	0.0	0.208101	128733	129380	769-769	0.333333	51.7
ndhF-rpl32_p4	ndhF-rpl32	4	TATGAGCCACCCGTATGGAT	TTGAACGCTGTCCAATACCC	20	20	50.0	50.0	58.0	58.2	1.3	0.0	0.208101	128733	129380	770-770	0.333333	51.7
ndhF-rpl32_p5	ndhF-rpl32	5	GAATTTTCTGTTGCCGTCGG	TTGAACGCTGTCCAATACCC	20	20	50.0	50.0	58.0	58.2	7.0	0.0	0.220179	128733	129380	844-844	0.333333	51.6
rps15-trnN-GUU_p1	rps15-trnN-GUU	1	CGACGCAGATTCACCCTATT	CCAATCCACTCGATTTCAACC	20	21	50.0	47.6	57.8	57.2	0.0	0.0	1.989953	141555	141674	533-533	0.333333	37.4
rps15-trnN-GUU_p2	rps15-trnN-GUU	2	CGACGCAGATTCACCCTATT	TGCCAGACTCAATACACAAGT	20	21	50.0	42.9	57.8	57.2	0.0	0.0	1.994807	141555	141674	642-642	0.333333	37.4
rps15-trnN-GUU_p3	rps15-trnN-GUU	3	TACGACGCAGATTCACCCTA	CCAATCCACTCGATTTCAACC	20	21	50.0	47.6	58.2	57.2	0.0	0.0	2.010955	141555	141674	535-535	0.333333	37.2
rps15-trnN-GUU_p4	rps15-trnN-GUU	4	TACGACGCAGATTCACCCTA	TGCCAGACTCAATACACAAGT	20	21	50.0	42.9	58.2	57.2	0.0	0.0	2.015808	141555	141674	644-644	0.333333	37.2
rps15-trnN-GUU_p5	rps15-trnN-GUU	5	TGCAGGACTCATTGATGCAG	CCAATCCACTCGATTTCAACC	20	21	50.0	47.6	58.3	57.2	8.3	0.0	2.018415	141555	141674	916-916	0.333333	37.2
trnL-CAA-ycf15_p1	trnL-CAA-ycf15	1	TGAGGAGAGCTTTGTGGAGA	ATACCCGTCACATGCTTTCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.053843	166609	167608	1309-1309	0.333333	52.9
trnL-CAA-ycf15_p2	trnL-CAA-ycf15	2	AGAAGGGGTTGGAGTCGTTA	ATACCCGTCACATGCTTTCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.058194	166609	167608	1191-1191	0.333333	52.9
trnL-CAA-ycf15_p3	trnL-CAA-ycf15	3	TGAGGAGAGCTTTGTGGAGA	TCGTTCCAGTGAAAAGGCAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.061018	166609	167608	1917-1917	0.333333	52.8
trnL-CAA-ycf15_p4	trnL-CAA-ycf15	4	TGAGGAGAGCTTTGTGGAGA	TTCGTTCCAGTGAAAAGGCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.061018	166609	167608	1918-1918	0.333333	52.8
trnL-CAA-ycf15_p5	trnL-CAA-ycf15	5	CATTCCGATGTAGTGGTGCA	ATACCCGTCACATGCTTTCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.061151	166609	167608	1210-1210	0.333333	52.8