primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	78	483	444-475	1.000000	85.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	78	483	458-489	1.000000	85.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	78	483	457-488	1.000000	85.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	78	483	459-490	1.000000	85.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	CGCATGGTGGATTCACAATC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.5	57.9	6.4	0.0	0.554952	78	483	473-504	1.000000	82.5
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4368	5188	943-956	1.000000	80.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4368	5188	942-955	1.000000	80.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAAGACCATAGCCGCACTT	AAAAAGGCGCTCAACCTACA	20	20	45.0	45.0	57.8	58.0	0.0	7.6	0.224713	4368	5188	964-977	1.000000	80.4
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.3	58.0	5.3	7.6	0.307146	4368	5188	952-965	1.000000	79.8
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	CTTGAAAAAGGCGCTCAACC	20	20	50.0	50.0	58.2	58.2	20.3	7.6	0.397463	4368	5188	946-959	1.000000	79.1
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6301	8214	1850-1987	1.000000	83.0
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GGGTACAAGAATCCACAGCC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.2	58.1	0.0	16.9	0.324664	6301	8214	1883-2020	1.000000	82.1
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	6301	8214	1854-1991	1.000000	81.9
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCACAGCCGATAATGTCCTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.7	58.1	0.0	16.9	0.408164	6301	8214	1871-2008	1.000000	81.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GTACAAGAATCCACAGCCGA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.6	58.1	0.0	16.9	0.456185	6301	8214	1881-2018	1.000000	81.0
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	ACGAATCACGCTTTTACCACT	20	21	45.0	42.9	58.0	58.2	0.0	0.0	1.275461	9522	10345	892-898	1.000000	71.2
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	ATTAGCAATCCGCCGCTTTA	ACGAATCACGCTTTTACCAC	20	20	45.0	45.0	58.0	56.8	0.0	0.0	1.282487	9522	10345	892-898	1.000000	71.2
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ATTAGCAATCCGCCGCTTTA	AACGAATCACGCTTTTACCAC	20	21	45.0	42.9	58.0	57.5	0.0	0.0	1.569469	9522	10345	893-899	1.000000	68.9
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	ATTAGCAATCCGCCGCTTTA	CGAATCACGCTTTTACCACT	20	20	45.0	45.0	58.0	56.5	0.0	0.0	1.572859	9522	10345	891-897	1.000000	68.9
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	GCTTTAGTCCACTCAGCCAT	ACGAATCACGCTTTTACCACT	20	21	50.0	42.9	57.6	58.2	0.9	0.0	1.647203	9522	10345	878-884	1.000000	68.3
rpoC2_p1	rpoC2	1	AATCGTGAGGGAGAAAGCAC	TTCGTTGGGTCCTTTAGGGA	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.368487	18651	18770	245-245	1.000000	78.0
rpoC2_p2	rpoC2	2	AATCGTGAGGGAGAAAGCAC	GATCCCGCGATTCCCATTAG	20	20	50.0	55.0	57.9	58.3	0.0	8.2	0.373751	18651	18770	268-268	1.000000	77.9
rpoC2_p3	rpoC2	3	GTGAGGGAGAAAGCACCAAT	TTCGTTGGGTCCTTTAGGGA	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.474459	18651	18770	241-241	1.000000	77.2
rpoC2_p4	rpoC2	4	GTGAGGGAGAAAGCACCAAT	GATCCCGCGATTCCCATTAG	20	20	50.0	55.0	57.8	58.3	0.0	8.2	0.479723	18651	18770	264-264	1.000000	77.1
rpoC2_p5	rpoC2	5	AATCGTGAGGGAGAAAGCAC	ATTCGTTGGGTCCTTTAGGG	20	20	50.0	50.0	57.9	57.2	0.0	0.0	0.901681	18651	18770	246-246	1.000000	73.8
rpoC2-rpoC1_p1	rpoC2-rpoC1	1	GGTGTGCGATGTATATGCCA	AGGCCTACTCATACGGTACC	20	20	50.0	55.0	58.1	58.0	0.0	1.3	0.129826	21720	21880	298-298	1.000000	85.7
rpoC2-rpoC1_p2	rpoC2-rpoC1	2	GGTGTGCGATGTATATGCCA	GGCCTACTCATACGGTACCT	20	20	50.0	55.0	58.1	58.0	0.0	10.9	0.129826	21720	21880	297-297	1.000000	85.7
rpoC2-rpoC1_p3	rpoC2-rpoC1	3	AGGGTGTGCGATGTATATGC	GGCCTACTCATACGGTACCT	20	20	50.0	55.0	57.8	58.0	0.0	10.9	0.177295	21720	21880	299-299	1.000000	85.3
rpoC2-rpoC1_p4	rpoC2-rpoC1	4	AGGGTGTGCGATGTATATGC	AGGCCTACTCATACGGTACC	20	20	50.0	55.0	57.8	58.0	0.0	1.3	0.177295	21720	21880	300-300	1.000000	85.2
rpoC2-rpoC1_p5	rpoC2-rpoC1	5	GGTGTGCGATGTATATGCCA	AGAAGAAGCCATACAGGGGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.149776	21720	21880	324-324	1.000000	85.0
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAATGAACAACGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	21	20	42.9	50.0	58.2	57.9	0.0	0.0	1.319742	31394	32365	1084-1140	1.000000	70.4
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	GAATGAACAACGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	56.7	57.9	0.0	0.0	1.336812	31394	32365	1083-1139	1.000000	70.3
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGAATGAACAACGCAGTAGCA	ATGCTGTACACCCCATTTCC	21	20	42.9	50.0	58.2	57.9	0.0	0.0	1.377563	31394	32365	1113-1169	1.000000	69.9
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	GAATGAACAACGCAGTAGCA	ATGCTGTACACCCCATTTCC	20	20	45.0	50.0	56.7	57.9	0.0	0.0	1.394634	31394	32365	1112-1168	1.000000	69.8
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	GAATGAACAACGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	21	20	42.9	50.0	57.5	57.9	0.0	0.0	1.622212	31394	32365	1083-1139	1.000000	68.0
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	32965	33688	824-830	1.000000	78.9
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	32965	33688	803-809	1.000000	78.8
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CAACCCCCTTTCGTCTTACC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.188613	32965	33688	866-871	0.666667	66.0
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CAACCCCCTTTCGTCTTACC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.246710	32965	33688	845-850	0.666667	65.5
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CAACCCCCTTTCGTCTTACC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.268865	32965	33688	842-847	0.666667	65.4
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TCTCCTGAGGTTGTCGGAAT	TTGTGGTCGGTTGGTGAATT	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.121466	46718	47530	1023-1035	1.000000	80.3
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TCTCCTGAGGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.212764	46718	47530	993-1005	1.000000	79.6
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	58.1	57.8	0.0	5.6	0.254079	46718	47530	943-955	1.000000	79.3
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TCTCCTGAGGTTGTCGGAAT	ATTGTGGTCGGTTGGTGAAT	20	20	50.0	45.0	58.1	57.7	0.0	0.0	0.347780	46718	47530	1024-1036	1.000000	78.5
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTCTCCTGAGGTTGTCGGAA	TTGTGGTCGGTTGGTGAATT	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.347994	46718	47530	1024-1036	1.000000	78.5
ycf4-cemA_p1	ycf4-cemA	1	AAAAAGCTGCTGAATTGGCC	AGATCCACCAGGGCAAAAAG	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.245495	63620	64530	1017-1036	1.000000	80.4
ycf4-cemA_p2	ycf4-cemA	2	AAAAAGCTGCTGAATTGGCC	GAGATCCACCAGGGCAAAAA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.251008	63620	64530	1018-1037	1.000000	80.3
ycf4-cemA_p3	ycf4-cemA	3	AAAAAGCTGCTGAATTGGCC	GATCCACCAGGGCAAAAAGA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.251008	63620	64530	1016-1035	1.000000	80.3
ycf4-cemA_p4	ycf4-cemA	4	AAAGCTGCTGAATTGGCCTA	AGATCCACCAGGGCAAAAAG	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.291785	63620	64530	1015-1034	1.000000	80.0
ycf4-cemA_p5	ycf4-cemA	5	AAAGCTGCTGAATTGGCCTA	GATCCACCAGGGCAAAAAGA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.297297	63620	64530	1014-1033	1.000000	80.0
petA-psbJ_p1	petA-psbJ	1	ACGTGTCCAAGGTTTGTTGT	GGCTCATATTCCGGATTGGG	20	20	45.0	55.0	58.2	58.2	0.0	4.1	0.344636	66406	67555	1294-1318	1.000000	80.3
petA-psbJ_p2	petA-psbJ	2	ACGTGTCCAAGGTTTGTTGT	CGGCTCATATTCCGGATTGG	20	20	45.0	55.0	58.2	58.6	0.0	4.1	0.728252	66406	67555	1295-1319	1.000000	77.2
petA-psbJ_p3	petA-psbJ	3	ACGTGTCCAAGGTTTGTTGT	GCTCATATTCCGGATTGGGA	20	20	45.0	50.0	58.2	57.1	0.0	4.1	1.030186	66406	67555	1293-1317	1.000000	74.8
petA-psbJ_p4	petA-psbJ	4	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	20	20	45.0	50.0	58.2	57.1	0.0	4.1	1.058172	66406	67555	1287-1311	1.000000	74.6
petA-psbJ_p5	petA-psbJ	5	CGTGTCCAAGGTTTGTTGTT	GGCTCATATTCCGGATTGGG	20	20	45.0	55.0	57.1	58.2	0.0	4.1	1.105127	66406	67555	1293-1317	1.000000	74.2
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.527577	68325	69574	1427-1449	1.000000	77.1
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.531926	68325	69574	1425-1447	1.000000	77.0
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AAGCAGCCAGTAGAAAACCG	20	20	45.0	50.0	58.2	58.5	0.0	0.0	0.647263	68325	69574	1436-1458	1.000000	76.1
psbE-petL_p4	psbE-petL	4	ACGAATAACCAACCCGCAAT	GCAGCCAGTAGAAAACCGAA	20	20	45.0	50.0	58.2	58.5	0.0	0.0	0.651612	68325	69574	1434-1456	1.000000	76.1
psbE-petL_p5	psbE-petL	5	TGCTGACGAATAACCAACCC	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.660168	68325	69574	1441-1463	1.000000	76.0
ycf2_p1	ycf2	1	CGACATTGCACGATCAAAGG	GCGAACCCCAGTTAGATTCA	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.560415	90385	90504	316-316	1.000000	76.8
ycf2_p2	ycf2	2	CGACATTGCACGATCAAAGG	GATCACATTCCTCCATCGCC	20	20	50.0	55.0	57.8	58.5	0.0	0.0	0.646584	90385	90504	336-336	1.000000	76.0
ycf2_p3	ycf2	3	CGACATTGCACGATCAAAGG	ACGATCACATTCCTCCATCG	20	20	50.0	50.0	57.8	57.5	0.0	0.0	0.679752	90385	90504	338-338	1.000000	75.7
ycf2_p4	ycf2	4	CGACATTGCACGATCAAAGG	CGCGAACCCCAGTTAGATTC	20	20	50.0	55.0	57.8	58.7	0.0	0.0	0.876401	90385	90504	317-317	1.000000	74.3
ycf2_p5	ycf2	5	CGACATTGCACGATCAAAGG	CGATCACATTCCTCCATCGC	20	20	50.0	55.0	57.8	58.9	0.0	0.0	1.020521	90385	90504	337-337	1.000000	73.0
ndhF-rpl32_p1	ndhF-rpl32	1	GGAACAAGCAGAAGTCCCAA	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.323995	116039	117265	1330-1361	1.000000	79.6
ndhF-rpl32_p2	ndhF-rpl32	2	AGCAGAAGTCCCAACCCTAT	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.332664	116039	117265	1324-1355	1.000000	79.6
ndhF-rpl32_p3	ndhF-rpl32	3	AAGCAGAAGTCCCAACCCTA	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	58.3	57.7	0.0	0.0	0.562944	116039	117265	1325-1356	1.000000	77.7
ndhF-rpl32_p4	ndhF-rpl32	4	GGAACAAGCAGAAGTCCCAA	GGCCTGATATCCCCTTCCTT	20	20	50.0	55.0	58.0	58.5	0.0	0.0	0.568384	116039	117265	1388-1419	1.000000	77.6
ndhF-rpl32_p5	ndhF-rpl32	5	AGCAGAAGTCCCAACCCTAT	GGCCTGATATCCCCTTCCTT	20	20	50.0	55.0	58.0	58.5	0.0	0.0	0.577053	116039	117265	1382-1413	1.000000	77.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CGCGGCAAAAGCTTTATCTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.7	57.9	13.1	0.0	0.386849	117440	118822	1512-1524	1.000000	79.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CGCGGCAAAAGCTTTATCTT	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	57.7	57.8	13.1	0.0	0.447576	117440	118822	1505-1517	1.000000	78.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CGCGGCAAAAGCTTTATCTT	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	57.7	57.8	13.1	0.0	0.458867	117440	118822	1511-1523	1.000000	78.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CGCGGCAAAAGCTTTATCTT	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	57.7	57.8	13.1	0.0	0.458867	117440	118822	1510-1522	1.000000	78.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	CGCGGCAAAAGCTTTATCTT	GAGCAGCGTGTCTACCAATT	20	20	45.0	50.0	57.7	58.3	13.1	0.0	0.535719	117440	118822	1506-1518	1.000000	77.8
ccsA-ndhD_p1	ccsA-ndhD	1	TGGATATGCTATTTTGGGGTCA	TCCTGATTTCGTTCTCTCGC	22	20	40.9	50.0	57.2	57.7	0.0	0.0	3.084722	119941	120174	369-387	1.000000	59.5
ccsA-ndhD_p2	ccsA-ndhD	2	TGGATATGCTATTTTGGGGTCA	CCTGATTTCGTTCTCTCGCT	22	20	40.9	50.0	57.2	57.7	0.0	0.0	3.090966	119941	120174	368-386	1.000000	59.5
ccsA-ndhD_p3	ccsA-ndhD	3	TGGATATGCTATTTTGGGGTCAA	TCCTGATTTCGTTCTCTCGC	23	20	39.1	50.0	57.9	57.7	0.0	0.0	3.415650	119941	120174	369-387	1.000000	56.9
ccsA-ndhD_p4	ccsA-ndhD	4	TTGGATATGCTATTTTGGGGTCA	TCCTGATTTCGTTCTCTCGC	23	20	39.1	50.0	57.9	57.7	0.0	0.0	3.415650	119941	120174	370-388	1.000000	56.9
ccsA-ndhD_p5	ccsA-ndhD	5	TGGATATGCTATTTTGGGGTCAA	CCTGATTTCGTTCTCTCGCT	23	20	39.1	50.0	57.9	57.7	0.0	0.0	3.421894	119941	120174	368-386	1.000000	56.8
ycf1_p1	ycf1	1	GTATTCTGATCCGGATCCGC	CGACCGAAAAACTATCCCCC	20	20	55.0	55.0	58.0	58.3	35.7	0.0	0.342164	132242	132391	781-799	1.000000	80.9
ycf1_p2	ycf1	2	GTATTCTGATCCGGATCCGC	GACCGAAAAACTATCCCCCG	20	20	55.0	55.0	58.0	58.3	35.7	0.0	0.342164	132242	132391	780-798	1.000000	80.9
ycf1_p3	ycf1	3	TTGCCTCTTCCTCCTCAGAA	CGACCGAAAAACTATCCCCC	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.369861	132242	132391	1083-1101	1.000000	80.1
ycf1_p4	ycf1	4	TTGCCTCTTCCTCCTCAGAA	GACCGAAAAACTATCCCCCG	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.369861	132242	132391	1082-1100	1.000000	80.1
ycf1_p5	ycf1	5	TTTGCCTCTTCCTCCTCAGA	GACCGAAAAACTATCCCCCG	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.369861	132242	132391	1083-1101	1.000000	80.1