primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	CAATCCACTGCCTTGATCCA	AGCTGCTGTTGAAGTTCCAT	20	20	50.0	45.0	57.9	57.7	0.0	0.0	0.416427	62	550	552-690	1.000000	84.0
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	AATCCACTGCCTTGATCCAC	AGCTGCTGTTGAAGTTCCAT	20	20	50.0	45.0	57.9	57.7	0.0	0.0	0.417934	62	550	551-689	1.000000	84.0
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	ACAATCCACTGCCTTGATCC	AGCTGCTGTTGAAGTTCCAT	20	20	50.0	45.0	57.9	57.7	0.0	0.0	0.417934	62	550	553-691	1.000000	84.0
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	CAATCCACTGCCTTGATCCA	GCTGCTGTTGAAGTTCCATC	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.759370	62	550	551-689	1.000000	81.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	AATCCACTGCCTTGATCCAC	GCTGCTGTTGAAGTTCCATC	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.760877	62	550	550-688	1.000000	81.3
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCTTTCAGGATCAGTCGTGG	AGCTCAACCCACAAGAACTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.042864	4547	5350	951-991	1.000000	89.5
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCTTTCAGGATCAGTCGTGG	AGAGATCTTCGGAGAGTGGG	20	20	55.0	55.0	58.0	57.9	0.0	0.0	0.081634	4547	5350	1040-1080	1.000000	89.1
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCAGGATCAGTCGTGGTCTT	AGCTCAACCCACAAGAACTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.102397	4547	5350	947-987	1.000000	89.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCTTTCAGGATCAGTCGTGG	TTCTTTCTTGCTTGAGCCGT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.048417	4547	5350	1166-1211	1.000000	88.9
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCTTTCAGGATCAGTCGTGG	GCCGTACGAGGAGAAAACTT	20	20	55.0	50.0	58.0	57.9	0.0	18.8	0.091715	4547	5350	1196-1196	0.500000	59.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6631	7365	808-1476	1.000000	86.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TGATCACGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.7	58.1	0.0	16.9	0.423798	6631	7365	822-1490	1.000000	84.6
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6631	7365	812-1480	1.000000	81.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.2	58.1	0.0	16.9	0.863824	6631	7365	800-1468	1.000000	81.3
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AGTCGCACGTTGCTTTCTAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.9	58.1	8.1	16.9	0.938588	6631	7365	807-1475	1.000000	80.6
trnS-GCU-trnS-CGA_p1	trnS-GCU-trnS-CGA	1	GCTTTAGTCCACTCAGCCAT	AGGACGAATCACACTTTTACCA	20	22	50.0	40.9	57.6	57.7	0.9	0.0	2.755807	8820	10649	1776-1893	1.000000	70.0
trnS-GCU-trnS-CGA_p2	trnS-GCU-trnS-CGA	2	GCTTTAGTCCACTCAGCCAT	GGACGAATCACACTTTTACCAC	20	22	50.0	45.5	57.6	57.6	0.9	0.0	2.809984	8820	10649	1775-1892	1.000000	69.5
trnS-GCU-trnS-CGA_p3	trnS-GCU-trnS-CGA	3	AGTCCACTCAGCCATCTCTC	AGGACGAATCACACTTTTACCA	20	22	55.0	40.9	58.5	57.7	0.0	0.0	2.861821	8820	10649	1771-1888	1.000000	69.1
trnS-GCU-trnS-CGA_p4	trnS-GCU-trnS-CGA	4	AGTCCACTCAGCCATCTCTC	GGACGAATCACACTTTTACCAC	20	22	55.0	45.5	58.5	57.6	0.0	0.0	2.915998	8820	10649	1770-1887	1.000000	68.7
trnS-GCU-trnS-CGA_p5	trnS-GCU-trnS-CGA	5	TAGTCCACTCAGCCATCTCT	AGGACGAATCACACTTTTACCA	20	22	50.0	40.9	57.2	57.7	0.0	0.0	3.182495	8820	10649	1772-1889	1.000000	66.5
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	33970	34767	851-885	1.000000	87.4
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	33970	34767	849-883	1.000000	87.4
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	33970	34767	828-862	1.000000	87.1
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	33970	34767	830-864	1.000000	87.0
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GATGTCCTGAACCGCTAGAC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.217323	33970	34767	848-882	1.000000	86.9
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	CCCCAGACGGCGTATTTTT	GATGGTCATCGGTTCGACTC	19	20	52.6	55.0	58.1	58.2	14.7	0.0	1.295812	49518	50020	583-741	1.000000	78.2
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	CCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	19	20	52.6	55.0	58.1	57.9	14.7	0.0	1.270002	49518	50020	614-772	1.000000	78.0
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	CCCCAGACGGCGTATTTTT	CCGCTTAGCTCAGAGGTTAG	19	20	52.6	55.0	58.1	57.5	14.7	7.6	1.637770	49518	50020	622-780	1.000000	75.0
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	CCCCAGACGGCGTATTTTT	CAGAGGTTAGAGCATCGCAT	19	20	52.6	50.0	58.1	57.5	14.7	0.0	1.660097	49518	50020	612-770	1.000000	74.9
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	CCCCAGACGGCGTATTTTT	TCAGAGGTTAGAGCATCGCA	19	20	52.6	50.0	58.1	58.5	14.7	0.0	1.662932	49518	50020	613-771	1.000000	74.9
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	51759	53113	944-1465	1.000000	88.3
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	51759	53113	987-1508	1.000000	86.8
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	51759	53113	986-1507	1.000000	86.8
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	CGCGCCTTAAACGTATCTTG	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.398918	51759	53113	969-1490	1.000000	86.4
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	TGGAAAATGGTCGGGATAGC	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.470617	51759	53113	992-1513	1.000000	85.7
accD-psaI_p1	accD-psaI	1	TCACAAGCGGCTGAGTATTC	GGAGTCACATGTCGTACCAC	20	20	50.0	55.0	58.0	58.0	1.8	0.0	0.017975	63605	64206	936-1652	1.000000	84.8
accD-psaI_p2	accD-psaI	2	TCACAAGCGGCTGAGTATTC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	1.8	26.9	0.037148	63605	64206	792-1509	1.000000	84.8
accD-psaI_p3	accD-psaI	3	TCACAAGCGGCTGAGTATTC	CCGCTTTTGACCAGAGCTAT	20	20	50.0	50.0	58.0	58.0	1.8	0.0	0.039983	63605	64206	1033-1749	1.000000	84.5
accD-psaI_p4	accD-psaI	4	AGTCGGCGAGAAAATAACCC	GGAGTCACATGTCGTACCAC	20	20	50.0	55.0	58.0	58.0	11.2	0.0	0.027778	63605	64206	1261-1261	0.500000	59.8
accD-psaI_p5	accD-psaI	5	AGTCGGCGAGAAAATAACCC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	11.2	26.9	0.046951	63605	64206	1117-1117	0.500000	59.6
petA-psbJ_p1	petA-psbJ	1	AGCACAAATCTTTTTGGTTCT	TCCGGATTAGGCTCATCTCT	21	20	33.3	50.0	54.5	57.3	3.8	0.0	5.226047	68152	69281	1193-1224	1.000000	49.5
petA-psbJ_p2	petA-psbJ	2	AGCACAAATCTTTTTGGTTCT	CCGGATTAGGCTCATCTCTG	21	20	33.3	55.0	54.5	57.3	3.8	8.5	5.280826	68152	69281	1192-1223	1.000000	49.5
petA-psbJ_p3	petA-psbJ	3	AGCACAAATCTTTTTGGTTCTT	TCCGGATTAGGCTCATCTCT	22	20	31.8	50.0	55.3	57.3	3.8	0.0	5.415396	68152	69281	1193-1224	1.000000	49.5
petA-psbJ_p4	petA-psbJ	4	AGCACAAATCTTTTTGGTTCTT	CCGGATTAGGCTCATCTCTG	22	20	31.8	55.0	55.3	57.3	3.8	8.5	5.470176	68152	69281	1192-1223	1.000000	49.5
petA-psbJ_p5	petA-psbJ	5	AGCACAAATCTTTTTGGTTCT	TTCCGGATTAGGCTCATCTCT	21	21	33.3	47.6	54.5	58.0	3.8	4.1	5.576732	68152	69281	1194-1225	1.000000	49.5
rpl16_p1	rpl16	1	ACACATCAATTCTCGAGCCC	TTCCCCTACAAACCATTCGC	20	20	50.0	50.0	58.0	58.2	7.8	0.0	0.200859	86949	88817	2181-2323	1.000000	90.0
rpl16_p2	rpl16	2	ATTGGGCACTGCTTCTTTGA	TTCCCCTACAAACCATTCGC	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.171373	86949	88817	2407-2407	0.500000	58.6
rpl16_p3	rpl16	3	TGGGCACTGCTTCTTTGATT	TTCCCCTACAAACCATTCGC	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.171373	86949	88817	2405-2405	0.500000	58.6
rpl16_p4	rpl16	4	TTGGGCACTGCTTCTTTGAT	TTCCCCTACAAACCATTCGC	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.171373	86949	88817	2406-2406	0.500000	58.6
rpl16_p5	rpl16	5	TCTCAATTCCCTGGCGATTG	TTCCCCTACAAACCATTCGC	20	20	50.0	50.0	58.0	58.2	11.7	0.0	0.213707	86949	88817	2581-2581	0.500000	58.3
trnL-CAA-ndhB_p1	trnL-CAA-ndhB	1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.0	57.9	5.8	29.4	0.064968	100611	101884	898-1334	1.000000	86.2
trnL-CAA-ndhB_p2	trnL-CAA-ndhB	2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.0	57.9	5.8	29.4	0.066338	100611	101884	896-1332	1.000000	86.2
trnL-CAA-ndhB_p3	trnL-CAA-ndhB	3	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.2	57.9	0.0	29.4	0.253803	100611	101884	893-1329	1.000000	84.7
trnL-CAA-ndhB_p4	trnL-CAA-ndhB	4	TCTCGCGTGTCTACCATTTC	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	57.7	57.9	5.8	29.4	0.330507	100611	101884	897-1333	1.000000	84.1
trnL-CAA-ndhB_p5	trnL-CAA-ndhB	5	TCGCGTGTCTACCATTTCAC	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.3	57.9	5.8	29.4	0.351881	100611	101884	895-1331	1.000000	83.9
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	CGGGAGAATTGATGACTGCA	TGGTTGGCTCCATCGAAAAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.040126	116186	117389	2056-2143	1.000000	91.7
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	GCCAGGTATTGACGGTGATT	TGGTTGGCTCCATCGAAAAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.050093	116186	117389	1941-2028	1.000000	91.6
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	CGGATCTACGCCAGGTATTG	TGGTTGGCTCCATCGAAAAA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.077699	116186	117389	1950-2037	1.000000	91.4
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	ATTATCCCGGATCTACGCCA	TGGTTGGCTCCATCGAAAAA	20	20	50.0	45.0	58.1	58.0	0.7	0.0	0.078685	116186	117389	1957-2044	1.000000	91.4
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	CGGGAGAATTGATGACTGCA	AGTTTCTTTGCGGGTGAACT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.081202	116186	117389	1684-1684	0.500000	59.4
psaC-ndhE_p1	psaC-ndhE	1	GGCCCGTACACATTGAGTAC	CGTAACAGAAAATCAACGCGT	20	21	55.0	42.9	58.4	57.8	12.7	5.6	1.515138	124950	125785	947-1089	1.000000	77.4
psaC-ndhE_p2	psaC-ndhE	2	GCCCGTACACATTGAGTACA	CGTAACAGAAAATCAACGCGT	20	21	50.0	42.9	57.6	57.8	19.8	5.6	1.537183	124950	125785	946-1088	1.000000	77.2
psaC-ndhE_p3	psaC-ndhE	3	AGGCCCGTACACATTGAGTA	CGTAACAGAAAATCAACGCGT	20	21	50.0	42.9	58.4	57.8	0.0	5.6	1.599718	124950	125785	948-1090	1.000000	76.7
psaC-ndhE_p4	psaC-ndhE	4	GGCCCGTACACATTGAGTAC	TCGTAACAGAAAATCAACGCG	20	21	55.0	42.9	58.4	57.6	12.7	3.5	1.779012	124950	125785	948-1090	1.000000	75.3
psaC-ndhE_p5	psaC-ndhE	5	GCCCGTACACATTGAGTACA	TCGTAACAGAAAATCAACGCG	20	21	50.0	42.9	57.6	57.6	19.8	3.5	1.801056	124950	125785	947-1089	1.000000	75.1
ndhG-ndhI_p1	ndhG-ndhI	1	TGTATTGGCCCAGTTAAATCCA	TTCAAGGACAGTTACCAATT	22	20	40.9	35.0	57.7	52.3	0.0	0.0	8.060100	127020	127415	441-540	1.000000	51.1
ndhG-ndhI_p2	ndhG-ndhI	2	TGTATTGGCCCAGTTAAATCCA	ATTCAAGGACAGTTACCAAT	22	20	40.9	35.0	57.7	52.0	0.0	0.0	8.312317	127020	127415	442-541	1.000000	51.1
ndhG-ndhI_p3	ndhG-ndhI	3	TGTATTGGCCCAGTTAAATCCA	ATTCAAGGACAGTTACCAATT	22	21	40.9	33.3	57.7	53.0	0.0	0.0	8.335217	127020	127415	442-541	1.000000	51.1
ndhG-ndhI_p4	ndhG-ndhI	4	TGTATTGGCCCAGTTAAATCCA	TCAAGGACAGTTACCAATTAG	22	21	40.9	38.1	57.7	52.9	0.0	0.0	8.444059	127020	127415	440-539	1.000000	51.1
ndhG-ndhI_p5	ndhG-ndhI	5	TGTATTGGCCCAGTTAAATCCA	TTCAAGGACAGTTACCAATTAG	22	22	40.9	36.4	57.7	53.8	0.0	0.0	8.563220	127020	127415	441-540	1.000000	51.1
rps15-ycf1_p1	rps15-ycf1	1	AACACCATGCATTGATGGGT	TCATTGAACCGGTTCGTCTC	20	20	45.0	50.0	58.1	57.9	8.9	35.4	0.133170	131831	132673	1877-2114	1.000000	87.9
rps15-ycf1_p2	rps15-ycf1	2	AACACCATGCATTGATGGGT	CATTGAACCGGTTCGTCTCT	20	20	45.0	50.0	58.1	57.9	8.9	35.4	0.138909	131831	132673	1876-2113	1.000000	87.9
rps15-ycf1_p3	rps15-ycf1	3	TCCCAACGCGTTACATAAGG	TCATTGAACCGGTTCGTCTC	20	20	50.0	50.0	58.0	57.9	26.1	35.4	0.087484	131831	132673	2014-2251	1.000000	87.8
rps15-ycf1_p4	rps15-ycf1	4	TCCCAACGCGTTACATAAGG	CATTGAACCGGTTCGTCTCT	20	20	50.0	50.0	58.0	57.9	26.1	35.4	0.093223	131831	132673	2013-2250	1.000000	87.8
rps15-ycf1_p5	rps15-ycf1	5	TTCCCAATTGTTCTGGTGCA	TCATTGAACCGGTTCGTCTC	20	20	45.0	50.0	57.9	57.9	4.4	35.4	0.153717	131831	132673	2073-2310	1.000000	87.1
ndhB-trnL-CAA_p1	ndhB-trnL-CAA	1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	57.9	58.0	29.4	5.8	0.064968	153103	154376	899-1334	1.000000	86.0
ndhB-trnL-CAA_p2	ndhB-trnL-CAA	2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	57.9	58.0	29.4	5.8	0.066338	153103	154376	897-1332	1.000000	86.0
ndhB-trnL-CAA_p3	ndhB-trnL-CAA	3	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	20	20	50.0	50.0	57.9	58.2	29.4	0.0	0.253803	153103	154376	894-1329	1.000000	84.5
ndhB-trnL-CAA_p4	ndhB-trnL-CAA	4	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	20	20	50.0	50.0	57.9	57.7	29.4	5.8	0.330507	153103	154376	898-1333	1.000000	83.9
ndhB-trnL-CAA_p5	ndhB-trnL-CAA	5	TGCAATTGCTCAGGATACCC	TCGCGTGTCTACCATTTCAC	20	20	50.0	50.0	57.9	58.3	29.4	5.8	0.351881	153103	154376	896-1331	1.000000	83.7