primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	ATATTATGGGCGAACGACGG	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.107227	102	362	438-442	1.000000	80.8
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	ATATTATGGGCGAACGACGG	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.140446	102	362	388-392	1.000000	80.7
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	ATATTATGGGCGAACGACGG	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.149276	102	362	391-395	1.000000	80.6
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	102	362	377-381	1.000000	80.6
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	102	362	391-395	1.000000	80.5
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	CGGAACTTCGCCCTAATCAA	20	20	50.0	50.0	58.2	58.0	20.3	0.0	0.186962	4277	5257	1045-1097	1.000000	82.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	CGGAACTTCGCCCTAATCAA	20	20	50.0	50.0	58.2	58.0	20.3	0.0	0.186962	4277	5257	1044-1096	1.000000	82.0
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCGCACTTAAAAGCCGAGTA	CGGAACTTCGCCCTAATCAA	20	20	50.0	50.0	58.3	58.0	5.3	0.0	0.301348	4277	5257	1054-1106	1.000000	81.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	58.2	20.3	1.3	0.358736	4277	5257	1100-1152	1.000000	80.5
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	58.2	20.3	1.3	0.358736	4277	5257	1099-1151	1.000000	80.5
rps16_p1	rps16	1	AGAAACAAAAAGGTTATACAAGGCT	TATGTACCGACCGAACCGAT	25	20	32.0	50.0	57.4	58.3	0.0	0.0	5.918149	5258	6391	1271-1274	1.000000	40.4
rps16_p2	rps16	2	AGAAACAAAAAGGTTATACAAGGCT	CCGACCGAACCGATGATTAT	25	20	32.0	50.0	57.4	57.6	0.0	0.0	6.031156	5258	6391	1265-1268	1.000000	40.4
rps16_p3	rps16	3	ACAAAAAGGTTATACAAGGCT	TATGTACCGACCGAACCGAT	21	20	33.3	50.0	53.3	58.3	0.0	0.0	6.032038	5258	6391	1267-1270	1.000000	40.4
rps16_p4	rps16	4	AGAAACAAAAAGGTTATACAAGGCT	ATTATGTACCGACCGAACCG	25	20	32.0	50.0	57.4	57.5	0.0	0.0	6.094395	5258	6391	1273-1276	1.000000	40.4
rps16_p5	rps16	5	GGAAATAGCAAAGAAACAAAAAGGT	TATGTACCGACCGAACCGAT	25	20	32.0	50.0	57.2	58.3	0.0	0.0	6.107039	5258	6391	1282-1285	1.000000	40.4
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6392	7222	889-915	1.000000	81.0
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GCTTTCTGCCACATCGTTTT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.6	58.1	0.0	16.9	0.498377	6392	7222	866-892	1.000000	79.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.6	58.1	23.3	16.9	0.491095	6392	7222	899-925	1.000000	79.2
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.4	58.1	23.3	16.9	0.499524	6392	7222	900-926	1.000000	79.2
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TCATGTCCTTCAAGTCGCAC	GTGTCGACTAGAAATGGGGC	20	20	50.0	55.0	58.2	58.3	0.0	17.9	0.551081	6392	7222	951-977	1.000000	78.6
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	AGCCTTCCAAGCTAACGATG	ACAATCTCAATCATCCCCGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056966	10172	11165	1111-1568	1.000000	85.1
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	CCTAGCCTTCCAAGCTAACG	ACAATCTCAATCATCCCCGC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.085803	10172	11165	1114-1571	1.000000	84.9
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	AGCCTTCCAAGCTAACGATG	TCAGACCGGCCATTAATGTG	20	20	50.0	50.0	58.0	58.0	0.0	14.3	0.066796	10172	11165	1316-1773	1.000000	84.8
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	AGCCTTCCAAGCTAACGATG	TTCGGTTTCCAGGGTAGGAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.068705	10172	11165	1291-1748	1.000000	84.8
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	CCTAGCCTTCCAAGCTAACG	TCAGACCGGCCATTAATGTG	20	20	55.0	50.0	58.1	58.0	0.0	14.3	0.095633	10172	11165	1319-1776	1.000000	84.6
atpH-atpI_p1	atpH-atpI	1	AATAGAAGCAAGCCCGACAG	GCGAATCCATGGAAGGTCAT	20	20	50.0	50.0	58.0	58.0	0.0	20.7	0.056966	14751	15936	1258-1284	1.000000	81.6
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	20	20	45.0	50.0	58.0	58.0	1.4	20.7	0.065515	14751	15936	1224-1250	1.000000	81.6
atpH-atpI_p3	atpH-atpI	3	AATAGAAGCAAGCCCGACAG	TTTTTGCAACCTTAGCTGCG	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.162578	14751	15936	1288-1314	1.000000	80.7
atpH-atpI_p4	atpH-atpI	4	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACCTTAGCTGCG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	14751	15936	1254-1280	1.000000	80.7
atpH-atpI_p5	atpH-atpI	5	AATAACGGAAGCGGCAGAAA	GCGAATCCATGGAAGGTCAT	20	20	45.0	50.0	58.2	58.0	1.4	20.7	0.211053	14751	15936	1225-1251	1.000000	80.4
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	TCACCTTGGCTGACTGTTTT	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	57.6	58.5	0.0	5.4	0.912704	31859	33106	1377-1416	1.000000	75.4
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AATCACCTTGGCTGACTGTT	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	57.3	58.5	0.0	5.4	1.141734	31859	33106	1379-1418	1.000000	73.5
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	ATCACCTTGGCTGACTGTTT	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	57.3	58.5	0.0	5.4	1.141734	31859	33106	1378-1417	1.000000	73.5
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	TCACCTTGGCTGACTGTTTT	GTTCAATTGGTCAGAGCACC	20	20	45.0	50.0	57.6	57.0	0.0	5.4	1.432858	31859	33106	1378-1417	1.000000	71.2
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	ATCACCTTGGCTGACTGTTTT	TTCAATTGGTCAGAGCACCG	21	20	42.9	50.0	58.1	58.5	0.0	5.4	1.527884	31859	33106	1378-1417	1.000000	70.5
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GGCCAGACCAACCTACAAAA	20	20	55.0	50.0	58.1	58.0	7.6	0.0	0.074361	34436	35915	1643-1659	1.000000	83.7
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GGCCAGACCAACCTACAAAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.096516	34436	35915	1646-1662	1.000000	83.5
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	TAGGCCAGACCAACCTACAA	20	20	55.0	50.0	58.1	58.0	7.6	5.0	0.097032	34436	35915	1645-1661	1.000000	83.5
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	20	20	55.0	45.0	58.1	58.0	7.6	0.0	0.106399	34436	35915	1633-1649	1.000000	83.4
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TCAGTGGTAGAGTAACGCCA	TAGGCCAGACCAACCTACAA	20	20	50.0	50.0	58.1	58.0	0.0	5.0	0.119187	34436	35915	1648-1664	1.000000	83.3
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	TAAGATCGCTTCCGGCTCTA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.155684	49294	49673	540-548	1.000000	79.8
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.196808	49294	49673	544-552	1.000000	79.4
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	CTTCCGGCTCTAGGCTTTTT	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.248083	49294	49673	532-540	1.000000	79.1
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.263437	49294	49673	508-516	1.000000	79.0
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	TTTCTAAGATCGCTTCCGGC	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.304561	49294	49673	512-520	1.000000	78.6
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	49747	50941	1232-1273	1.000000	81.7
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	49747	50941	1240-1281	1.000000	81.2
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	49747	50941	1264-1305	1.000000	80.8
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	49747	50941	1272-1313	1.000000	80.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	49747	50941	1246-1287	1.000000	79.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGGGCTAAAACTCCGGAAAT	GCACGTTGGGTCTTTGAAAC	20	20	50.0	50.0	57.9	58.2	5.2	0.0	0.304202	54423	55034	807-874	1.000000	81.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	5.2	0.0	0.336606	54423	55034	743-810	1.000000	81.1
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GGGGCTAAAACTCCGGAAAT	AATGCACGTTGGGTCTTTGA	20	20	50.0	45.0	57.9	58.3	5.2	0.0	0.452969	54423	55034	810-877	1.000000	79.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGGGCTAAAACTCCGGAAAT	ATGCACGTTGGGTCTTTGAA	20	20	50.0	45.0	57.9	58.3	5.2	0.0	0.452969	54423	55034	809-876	1.000000	79.9
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CTGGTCCTTTGCTAATCGGG	GCACGTTGGGTCTTTGAAAC	20	20	55.0	50.0	58.3	58.2	0.0	0.0	0.496460	54423	55034	824-891	1.000000	79.5
petA-psbJ_p1	petA-psbJ	1	TGCTCTTCTTGGCATCTGTT	AGCCGGTATTCTTGTGATCG	20	20	45.0	50.0	57.4	57.8	0.0	1.9	0.799074	66634	67612	1150-1159	1.000000	74.0
petA-psbJ_p2	petA-psbJ	2	TTGCTCTTCTTGGCATCTGT	AGCCGGTATTCTTGTGATCG	20	20	45.0	50.0	57.4	57.8	0.0	1.9	0.799074	66634	67612	1151-1160	1.000000	74.0
petA-psbJ_p3	petA-psbJ	3	CGTGTCCAAGGTCTTTTGCT	AGCCGGTATTCTTGTGATCG	20	20	50.0	50.0	58.7	57.8	0.0	1.9	0.916070	66634	67612	1166-1175	1.000000	73.1
petA-psbJ_p4	petA-psbJ	4	GGTCTTTTGCTCTTCTTGGC	AGCCGGTATTCTTGTGATCG	20	20	50.0	50.0	57.3	57.8	0.0	1.9	0.938964	66634	67612	1157-1166	1.000000	72.9
petA-psbJ_p5	petA-psbJ	5	TCCATTACGTGTCCAAGGTC	AGCCGGTATTCTTGTGATCG	20	20	50.0	50.0	57.2	57.8	0.0	1.9	0.980015	66634	67612	1173-1182	1.000000	72.6
petL-petG_p1	petL-petG	1	CGGTCTGAGCAAGATACGAC	CAACTGATCACCACGTCTGT	20	20	55.0	50.0	58.2	57.8	7.7	0.0	0.326022	69718	69875	291-291	1.000000	77.7
petL-petG_p2	petL-petG	2	TCGGTCTGAGCAAGATACGA	CAACTGATCACCACGTCTGT	20	20	50.0	50.0	58.3	57.8	7.7	0.0	0.408384	69718	69875	292-292	1.000000	77.0
petL-petG_p3	petL-petG	3	CGGTCTGAGCAAGATACGAC	ATCCAACTGATCACCACGTC	20	20	55.0	50.0	58.2	57.6	7.7	0.0	0.557829	69718	69875	294-294	1.000000	75.8
petL-petG_p4	petL-petG	4	TCGGTCTGAGCAAGATACGA	ATCCAACTGATCACCACGTC	20	20	50.0	50.0	58.3	57.6	7.7	0.0	0.640191	69718	69875	295-295	1.000000	75.1
petL-petG_p5	petL-petG	5	CGGTCTGAGCAAGATACGAC	CCAACTGATCACCACGTCTG	20	20	55.0	55.0	58.2	58.6	7.7	0.0	0.738654	69718	69875	292-292	1.000000	74.4
petG-trnW-CCA_p1	petG-trnW-CCA	1	ACAGACGTGGTGATCAGTTG	TTCAAATCCTACAGGGCGTG	20	20	50.0	50.0	57.8	57.9	0.0	5.5	0.262804	69990	70113	173-173	1.000000	85.3
petG-trnW-CCA_p2	petG-trnW-CCA	2	ACAGACGTGGTGATCAGTTG	GTTCAAATCCTACAGGGCGT	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.264278	69990	70113	174-174	1.000000	85.2
petG-trnW-CCA_p3	petG-trnW-CCA	3	ACAGACGTGGTGATCAGTTG	AGAACGTGGGTCTCCAAAAC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.191682	69990	70113	206-206	1.000000	84.7
petG-trnW-CCA_p4	petG-trnW-CCA	4	ACAGACGTGGTGATCAGTTG	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	57.8	57.9	0.0	1.4	0.243803	69990	70113	192-192	1.000000	84.7
petG-trnW-CCA_p5	petG-trnW-CCA	5	ACAGACGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	57.8	58.0	0.0	2.6	0.170881	69990	70113	228-228	1.000000	84.2
rpl20-rps12_p1	rpl20-rps12	1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	72772	73543	926-928	1.000000	75.3
rpl20-rps12_p2	rpl20-rps12	2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	72772	73543	925-927	1.000000	75.3
rpl20-rps12_p3	rpl20-rps12	3	CTTGAATGAGCCCCTCGAAA	CAGCGACGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.844694	72772	73543	889-891	1.000000	73.8
rpl20-rps12_p4	rpl20-rps12	4	TTGAATGAGCCCCTCGAAAG	CAGCGACGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.844694	72772	73543	888-890	1.000000	73.8
rpl20-rps12_p5	rpl20-rps12	5	TTGAATGAGCCCCTCGAAAG	AGCGACGAGGAACATGTACT	20	20	50.0	50.0	57.9	58.8	0.0	0.0	0.939077	72772	73543	887-889	1.000000	73.0
ndhF-rpl32_p1	ndhF-rpl32	1	TTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	20	20	40.0	45.0	57.0	57.8	0.0	13.1	1.214860	116869	117986	1299-1314	1.000000	75.8
ndhF-rpl32_p2	ndhF-rpl32	2	TTGTTGCCGTTGGAAAAAGT	AAGCTTTCAATGCTGCCCTA	20	20	40.0	45.0	57.0	57.8	0.0	0.0	1.261149	116869	117986	1297-1312	1.000000	75.4
ndhF-rpl32_p3	ndhF-rpl32	3	TTGTTGCCGTTGGAAAAAGT	GCTGCCCTATATCCCTTCCT	20	20	40.0	55.0	57.0	58.3	0.0	0.0	1.377810	116869	117986	1286-1301	1.000000	74.5
ndhF-rpl32_p4	ndhF-rpl32	4	TTGTTGCCGTTGGAAAAAGT	AGCTTTCAATGCTGCCCTAT	20	20	40.0	45.0	57.0	57.6	0.0	0.0	1.489633	116869	117986	1296-1311	1.000000	73.6
ndhF-rpl32_p5	ndhF-rpl32	5	TTTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	21	20	38.1	45.0	57.7	57.8	0.0	13.1	1.495037	116869	117986	1300-1315	1.000000	73.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGCAGCATTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.8	58.0	14.0	0.0	0.182194	118146	119624	1571-1654	1.000000	83.8
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGCAGCATTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.8	58.0	14.0	0.0	0.183564	118146	119624	1572-1655	1.000000	83.8
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TAGGGCAGCATTGAAAGCTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.228483	118146	119624	1573-1656	1.000000	83.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TAGGGCAGCATTGAAAGCTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.229854	118146	119624	1574-1657	1.000000	83.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGCAGCATTGAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.8	57.9	14.0	0.0	0.298641	118146	119624	1581-1664	1.000000	82.8