primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	AACAAGAGGGAAGCTATTGC	TTGAAAATTCGTGCGCTTGG	20	20	45.0	45.0	55.7	58.2	0.0	9.7	2.521655	473	1534	1180-1180	1.000000	60.0
psbA_p2	psbA	2	AACAAGAGGGAAGCTATTGC	ATATTGGTTGACACGGGCAT	20	20	45.0	45.0	55.7	57.6	0.0	0.0	2.721878	473	1534	1245-1245	1.000000	58.4
psbA_p3	psbA	3	AACAAGAGGGAAGCTATTGC	ATTGGTTGACACGGGCATAT	20	20	45.0	45.0	55.7	57.6	0.0	0.0	2.721878	473	1534	1243-1243	1.000000	58.4
psbA_p4	psbA	4	AAACAAGAGGGAAGCTATTGC	TTGAAAATTCGTGCGCTTGG	21	20	42.9	45.0	56.5	58.2	0.0	9.7	2.738574	473	1534	1181-1181	1.000000	58.2
psbA_p5	psbA	5	AAACAAGAGGGAAGCTATTGC	ATTGGTTGACACGGGCATAT	21	20	42.9	45.0	56.5	57.6	0.0	0.0	2.938798	473	1534	1244-1244	1.000000	56.6
trnK-UUU_p1	trnK-UUU	1	GCCCGTGTCAACCAATATCT	TCTGATTGTATCTACATACCCT	20	22	50.0	36.4	58.0	53.2	0.0	0.0	6.831189	1753	4358	2754-2755	1.000000	40.1
trnK-UUU_p2	trnK-UUU	2	GCCCGTGTCAACCAATATCT	TCTGATTGTATCTACATACCCTT	20	23	50.0	34.8	58.0	54.1	0.0	0.0	6.984390	1753	4358	2754-2755	1.000000	40.1
trnK-UUU_p3	trnK-UUU	3	GCCCGTGTCAACCAATATCT	TTCTGATTGTATCTACATACCCT	20	23	50.0	34.8	58.0	54.1	0.0	0.0	6.984390	1753	4358	2755-2756	1.000000	40.1
trnK-UUU_p4	trnK-UUU	4	TGCCCGTGTCAACCAATATC	TCTGATTGTATCTACATACCCT	20	22	50.0	36.4	58.3	53.2	0.0	0.0	7.047570	1753	4358	2755-2756	1.000000	40.1
trnK-UUU_p5	trnK-UUU	5	TGCCCGTGTCAACCAATATC	TCTGATTGTATCTACATACCCTT	20	23	50.0	34.8	58.3	54.1	0.0	0.0	7.200771	1753	4358	2755-2756	1.000000	40.1
matK_p1	matK	1	GTGATCAAATTTTTATTTACAACG	ACCCCCAAAATCCTCTACCT	24	20	25.0	50.0	52.1	57.6	0.0	0.0	10.281302	2067	3587	1598-1598	1.000000	40.2
matK_p2	matK	2	GTGATCAAATTTTTATTTACAACG	AACCCCCAAAATCCTCTACC	24	20	25.0	50.0	52.1	56.8	0.0	0.0	11.121642	2067	3587	1599-1599	1.000000	40.2
matK_p3	matK	3	GTGATCAAATTTTTATTTACAACG	AACCCCCAAAATCCTCTACCT	24	21	25.0	47.6	52.1	58.3	0.0	0.0	11.247080	2067	3587	1599-1599	1.000000	40.2
matK_p4	matK	4	GTGATCAAATTTTTATTTACAACG	ACCCCCAAAATCCTCTACCTT	24	21	25.0	47.6	52.1	58.3	0.0	0.0	11.247080	2067	3587	1598-1598	1.000000	40.2
matK_p5	matK	5	GTGATCAAATTTTTATTTACAACG	CCCCCAAAATCCTCTACCTT	24	20	25.0	50.0	52.1	56.5	0.0	0.0	11.428586	2067	3587	1597-1597	1.000000	40.2
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCGATGGTATGGACGAATCC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	0.0	7.6	0.393931	4359	5153	992-992	1.000000	76.9
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CGATGGTATGGACGAATCCC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	0.0	7.6	0.393931	4359	5153	991-991	1.000000	76.9
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4359	5153	931-931	1.000000	76.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4359	5153	930-930	1.000000	76.2
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.8	58.3	0.0	7.6	0.547945	4359	5153	1016-1016	1.000000	75.7
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6264	7198	1008-1045	1.000000	78.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TGCTTTCTACCGCATCGTTT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.2	58.1	0.0	16.9	0.272767	6264	7198	997-1034	1.000000	77.9
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TTGCTTTCTACCGCATCGTT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.2	58.1	0.0	16.9	0.272767	6264	7198	998-1035	1.000000	77.9
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6264	7198	1023-1060	1.000000	76.5
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6264	7198	1022-1059	1.000000	76.5
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	9237	10071	888-891	1.000000	78.5
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	9237	10071	906-909	1.000000	76.3
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	9237	10071	887-890	1.000000	72.6
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	20	20	50.0	50.0	58.1	58.9	0.0	0.0	0.984078	9237	10071	896-899	1.000000	72.5
trnC-GCA-petN_p5	trnC-GCA-petN	5	ATTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.1	58.2	0.0	0.0	1.063167	9237	10071	945-948	1.000000	71.8
rpoB_p1	rpoB	1	GCAAGGATATGGGTTCGTGT	ATTTCGTCGACCAATCCCTC	20	20	50.0	50.0	58.0	57.7	0.0	10.6	0.349511	13404	13523	294-294	1.000000	77.7
rpoB_p2	rpoB	2	GCAAGGATATGGGTTCGTGT	CGACCAATCCCTCCCAATTC	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.358049	13404	13523	287-287	1.000000	77.7
rpoB_p3	rpoB	3	GTTCGTGTGAGTAGGCAACA	ATTTCGTCGACCAATCCCTC	20	20	50.0	50.0	58.1	57.7	0.0	10.6	0.450448	13404	13523	282-282	1.000000	77.0
rpoB_p4	rpoB	4	GTTCGTGTGAGTAGGCAACA	CGACCAATCCCTCCCAATTC	20	20	50.0	55.0	58.1	58.3	0.0	0.0	0.458986	13404	13523	275-275	1.000000	76.9
rpoB_p5	rpoB	5	GCAAGGATATGGGTTCGTGT	CCAATCCCTCCCAATTCACA	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.568847	13404	13523	284-284	1.000000	76.0
atpI-atpH_p1	atpI-atpH	1	TCCCTGTCATGTTCCTTGGA	TACCTTGACCAACTCCAGGT	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.371251	25076	26212	1329-1330	1.000000	77.1
atpI-atpH_p2	atpI-atpH	2	TCCCTGTCATGTTCCTTGGA	GCAGTACCTTGACCAACTCC	20	20	50.0	55.0	58.3	58.2	0.0	0.0	0.449288	25076	26212	1333-1334	1.000000	76.5
atpI-atpH_p3	atpI-atpH	3	TTTTTGCAACTTTAGCCGCG	TACCTTGACCAACTCCAGGT	20	20	45.0	50.0	58.5	57.9	0.0	0.0	0.630002	25076	26212	1281-1282	1.000000	75.0
atpI-atpH_p4	atpI-atpH	4	TCCCTGTCATGTTCCTTGGA	AGGTCCAATAGAAGCAAGCC	20	20	50.0	50.0	58.3	57.6	0.0	0.0	0.685814	25076	26212	1313-1314	1.000000	74.6
atpI-atpH_p5	atpI-atpH	5	TCCCTGTCATGTTCCTTGGA	GTCCAATAGAAGCAAGCCCT	20	20	50.0	50.0	58.3	57.6	0.0	0.0	0.685814	25076	26212	1311-1312	1.000000	74.6
trnG-UCC_p1	trnG-UCC	1	TGATGAATGCTGTATTAATTAGAGTCT	ACATATACAGAAGCGCCTCG	27	20	29.6	50.0	56.7	57.6	0.0	2.3	8.705630	30105	30889	934-934	1.000000	40.1
trnG-UCC_p2	trnG-UCC	2	TGATGAATGCTGTATTAATTAGAGTCT	GCCTCGACAAAAGGTCCATT	27	20	29.6	50.0	56.7	58.5	0.0	0.0	8.746976	30105	30889	920-920	1.000000	40.1
trnG-UCC_p3	trnG-UCC	3	TGAATGCTGTATTAATTAGAGTCT	ACATATACAGAAGCGCCTCG	24	20	29.2	50.0	53.6	57.6	0.0	2.3	8.826085	30105	30889	931-931	1.000000	40.1
trnG-UCC_p4	trnG-UCC	4	TGAATGCTGTATTAATTAGAGTCT	GCCTCGACAAAAGGTCCATT	24	20	29.2	50.0	53.6	58.5	0.0	0.0	8.867431	30105	30889	917-917	1.000000	40.1
trnG-UCC_p5	trnG-UCC	5	TGATGAATGCTGTATTAATTAGAGTCT	TATACAGAAGCGCCTCGACA	27	20	29.6	50.0	56.7	58.6	0.0	2.3	8.896176	30105	30889	931-931	1.000000	40.1
psbC_p1	psbC	1	GTTTCTCAGGAAATTCGCGC	GAGGAAACCGAAGTGACTCC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.198249	33343	34764	1579-1579	1.000000	78.5
psbC_p2	psbC	2	CGTTTCTCAGGAAATTCGCG	GAGGAAACCGAAGTGACTCC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.226778	33343	34764	1580-1580	1.000000	78.2
psbC_p3	psbC	3	GTTTCTCAGGAAATTCGCGC	AGGAAACCGAAGTGACTCCT	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.398244	33343	34764	1578-1578	1.000000	76.9
psbC_p4	psbC	4	CGGCGGAAGATCCTGAATTT	GAGGAAACCGAAGTGACTCC	20	20	50.0	55.0	58.3	57.9	0.0	0.0	0.413306	33343	34764	1560-1560	1.000000	76.7
psbC_p5	psbC	5	AGGTATTCGTGCTTGGATGG	GAGGAAACCGAAGTGACTCC	20	20	50.0	55.0	57.7	57.9	0.0	0.0	0.413788	33343	34764	1505-1505	1.000000	76.7
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.273485	47830	48522	777-796	1.000000	71.7
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.275704	47830	48522	776-795	1.000000	71.7
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGATGGCC	20	21	50.0	47.6	58.1	57.7	0.0	0.0	1.349030	47830	48522	784-803	1.000000	71.1
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTACG	20	21	50.0	47.6	58.1	57.5	0.0	3.3	1.537359	47830	48522	778-797	1.000000	69.6
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACGT	20	21	50.0	47.6	58.1	58.6	0.0	3.3	1.647337	47830	48522	777-796	1.000000	68.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GTCCTTTGCTAATCGGAGCT	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.425062	50195	51342	1296-1296	1.000000	76.7
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CGGTCCTTTGCTAATCGGAG	CCGAGAAAGTCTACGGTTCG	20	20	55.0	55.0	58.4	58.1	0.0	0.0	0.535614	50195	51342	1298-1298	1.000000	75.8
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CTCCGGTCCTTTGCTAATCG	CCGAGAAAGTCTACGGTTCG	20	20	55.0	55.0	58.4	58.1	0.0	0.0	0.535614	50195	51342	1301-1301	1.000000	75.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGTCCTTTGCTAATCGGAGC	CCGAGAAAGTCTACGGTTCG	20	20	55.0	55.0	58.7	58.1	3.4	0.0	0.807051	50195	51342	1297-1297	1.000000	73.6
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GTCCTTTGCTAATCGGAGCT	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	57.7	58.7	0.0	0.0	1.000879	50195	51342	1281-1281	1.000000	72.1
atpB-rbcL_p1	atpB-rbcL	1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.047103	54339	55111	917-931	1.000000	72.8
atpB-rbcL_p2	atpB-rbcL	2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	20	21	50.0	42.9	58.0	58.1	0.0	0.0	1.080391	54339	55111	916-930	1.000000	72.6
atpB-rbcL_p3	atpB-rbcL	3	GGAAACCCCAGAACCAGAAG	CACCAGCTTTGAATCCAACAC	20	21	55.0	47.6	58.1	58.0	0.0	0.0	1.106208	54339	55111	844-858	1.000000	72.5
atpB-rbcL_p4	atpB-rbcL	4	GGAAACCCCAGAACCAGAAG	ACCAGCTTTGAATCCAACACT	20	21	55.0	42.9	58.1	58.1	0.0	0.0	1.139495	54339	55111	843-857	1.000000	72.2
atpB-rbcL_p5	atpB-rbcL	5	GATACGCCCCAGGTTCTTTT	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	57.9	58.0	0.0	0.0	1.141542	54339	55111	874-888	1.000000	72.1
rpl20_p1	rpl20	1	GACCTTCCCGGAGTTCATTC	ACTAGGAAAGGGGCAAAAGA	20	20	55.0	45.0	58.0	56.3	0.7	0.0	1.695079	68483	68863	489-489	1.000000	66.6
rpl20_p2	rpl20	2	TATTCGACCTTCCCGGAGTT	ACTAGGAAAGGGGCAAAAGA	20	20	50.0	45.0	58.1	56.3	0.7	0.0	1.812861	68483	68863	494-494	1.000000	65.7
rpl20_p3	rpl20	3	TTATTCGACCTTCCCGGAGT	ACTAGGAAAGGGGCAAAAGA	20	20	50.0	45.0	58.1	56.3	0.7	0.0	1.812861	68483	68863	495-495	1.000000	65.7
rpl20_p4	rpl20	4	GACCTTCCCGGAGTTCATTC	ACTAGGAAAGGGGCAAAAGAA	20	21	55.0	42.9	58.0	57.1	0.7	0.0	1.921292	68483	68863	489-489	1.000000	64.8
rpl20_p5	rpl20	5	CCTTCCCGGAGTTCATTCTC	ACTAGGAAAGGGGCAAAAGA	20	20	55.0	45.0	57.7	56.3	0.7	0.0	1.987020	68483	68863	487-487	1.000000	64.3
ycf1_p1	ycf1	1	CACACTAAGCGACGACTCTC	TGGAATTGTCTTTCCTTTTAAAAGT	20	25	55.0	28.0	58.1	55.8	0.0	23.0	7.300649	111210	111329	285-285	1.000000	40.6
ycf1_p2	ycf1	2	ACACTAAGCGACGACTCTCT	TGGAATTGTCTTTCCTTTTAAAAGT	20	25	50.0	28.0	58.2	55.8	0.0	23.0	7.374446	111210	111329	284-284	1.000000	40.6
ycf1_p3	ycf1	3	CACACTAAGCGACGACTCTC	TGGAATTGTCTTTCCTTTTAAAAGTTG	20	27	55.0	29.6	58.1	57.6	0.0	23.0	7.492557	111210	111329	285-285	1.000000	40.6
ycf1_p4	ycf1	4	CACACTAAGCGACGACTCTC	GGAATTGTCTTTCCTTTTAAAAGTTGT	20	27	55.0	29.6	58.1	57.6	0.0	23.0	7.493795	111210	111329	284-284	1.000000	40.6
ycf1_p5	ycf1	5	ACACTAAGCGACGACTCTCT	TGGAATTGTCTTTCCTTTTAAAAGTTG	20	27	50.0	29.6	58.2	57.6	0.0	23.0	7.566354	111210	111329	284-284	1.000000	40.6
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AGCGTGTCTACCGATTTCAC	AAGGAAAGGATATTGGGCGG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.372803	123649	124514	998-998	1.000000	77.3
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CAGCGTGTCTACCGATTTCA	AAGGAAAGGATATTGGGCGG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.374173	123649	124514	999-999	1.000000	77.3
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AGCGTGTCTACCGATTTCAC	CGGCGTTAAAAGCTTTGTCA	20	20	50.0	45.0	58.0	57.6	0.0	13.5	0.400763	123649	124514	981-981	1.000000	77.1
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	CAGCGTGTCTACCGATTTCA	CGGCGTTAAAAGCTTTGTCA	20	20	50.0	45.0	58.0	57.6	0.0	13.5	0.402133	123649	124514	982-982	1.000000	77.1
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	TCCTAAGAGCAGCGTGTCTA	AAGGAAAGGATATTGGGCGG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.489250	123649	124514	1008-1008	1.000000	76.4
ndhF-trnN-GUU_p1	ndhF-trnN-GUU	1	GGGTGGGCGTATTTCTTCTT	AGGATTCGAACCTACGACCA	20	20	50.0	50.0	57.9	58.2	0.0	15.0	0.280909	127968	128873	1053-1053	1.000000	77.9
ndhF-trnN-GUU_p2	ndhF-trnN-GUU	2	GGGTGGGCGTATTTCTTCTT	CGAGGTGTTGACGGTGATTT	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.615645	127968	128873	1089-1089	1.000000	75.2
ndhF-trnN-GUU_p3	ndhF-trnN-GUU	3	GGGTGGGCGTATTTCTTCTT	GGATTCGAACCTACGACCAA	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.784466	127968	128873	1052-1052	1.000000	73.9
ndhF-trnN-GUU_p4	ndhF-trnN-GUU	4	GGGTGGGCGTATTTCTTCTT	GAGTATCCCGGACCTACACC	20	20	50.0	60.0	57.9	58.7	0.0	0.7	0.797949	127968	128873	1108-1108	1.000000	73.8
ndhF-trnN-GUU_p5	ndhF-trnN-GUU	5	TTAAATCGGTAGGGGGTGGG	AGGATTCGAACCTACGACCA	20	20	55.0	50.0	58.8	58.2	0.0	15.0	0.941491	127968	128873	1066-1066	1.000000	72.6