primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbB-clpP_p1	psbB-clpP	1	AATGCGCCAAAACCAAAACA	TTATCTCTAGAGCGGGTGGG	20	20	40.0	55.0	58.0	58.0	4.2	15.4	0.033040	20952	21961	1721-1725	1.000000	80.1
psbB-clpP_p2	psbB-clpP	2	ATGCGCCAAAACCAAAACAA	TTATCTCTAGAGCGGGTGGG	20	20	40.0	55.0	58.0	58.0	4.2	15.4	0.033040	20952	21961	1720-1724	1.000000	80.1
psbB-clpP_p3	psbB-clpP	3	ATCCCAATACACCCAATGCC	TTATCTCTAGAGCGGGTGGG	20	20	50.0	55.0	57.9	58.0	0.0	15.4	0.089812	20952	21961	1611-1615	1.000000	79.6
psbB-clpP_p4	psbB-clpP	4	AATGCGCCAAAACCAAAACA	ATCTGCCCATAGAACCGAGA	20	20	40.0	50.0	58.0	57.9	4.2	0.0	0.117943	20952	21961	1545-1546	1.000000	79.4
psbB-clpP_p5	psbB-clpP	5	ATGCGCCAAAACCAAAACAA	ATCTGCCCATAGAACCGAGA	20	20	40.0	50.0	58.0	57.9	4.2	0.0	0.117943	20952	21961	1544-1545	1.000000	79.4
atpF_p1	atpF	1	AGGGTTGCCATGAGTCTTTC	AGTAGCAAAGAGGTGAAGCA	20	20	50.0	45.0	57.8	56.8	0.0	0.0	1.437057	24988	26288	1439-1441	1.000000	68.9
atpF_p2	atpF	2	GAAGGGTTGCCATGAGTCTT	AGTAGCAAAGAGGTGAAGCA	20	20	50.0	45.0	57.8	56.8	0.0	0.0	1.437057	24988	26288	1441-1443	1.000000	68.9
atpF_p3	atpF	3	GGGTTGCCATGAGTCTTTCT	AGTAGCAAAGAGGTGAAGCA	20	20	50.0	45.0	57.8	56.8	0.0	0.0	1.437057	24988	26288	1438-1440	1.000000	68.9
atpF_p4	atpF	4	GGGTTGCCATGAGTCTTTCT	AAGTAGCAAAGAGGTGAAGCA	20	21	50.0	42.9	57.8	57.5	0.0	0.0	1.698678	24988	26288	1439-1441	1.000000	66.9
atpF_p5	atpF	5	GGGTTGCCATGAGTCTTTCT	AGTAGCAAAGAGGTGAAGCAA	20	21	50.0	42.9	57.8	57.5	0.0	0.0	1.698678	24988	26288	1438-1440	1.000000	66.9
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAATGAAGAGTGCAGTAGC	GTTCCCCGGGATTGTAGTTC	20	20	45.0	55.0	55.2	58.0	0.0	26.3	2.852495	43059	43932	1002-1002	1.000000	57.3
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	ACTAGAATGAAGAGTGCAGTAGC	GTTCCCCGGGATTGTAGTTC	23	20	43.5	55.0	58.0	58.0	0.0	26.3	3.039026	43059	43932	1005-1005	1.000000	55.8
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGGAACTAGAATGAAGAGTGCAG	GTTCCCCGGGATTGTAGTTC	23	20	43.5	55.0	57.9	58.0	0.0	26.3	3.117638	43059	43932	1009-1009	1.000000	55.2
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGAATGAAGAGTGCAGTAGC	AGTTCCCCGGGATTGTAGTT	20	20	45.0	50.0	55.2	58.3	0.0	26.3	3.153253	43059	43932	1003-1003	1.000000	54.9
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGAATGAAGAGTGCAGTAGC	TCCCCGGGATTGTAGTTCAA	20	20	45.0	50.0	55.2	58.3	0.0	26.3	3.159369	43059	43932	1000-1000	1.000000	54.9
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	44618	45395	882-917	1.000000	78.8
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.233900	44618	45395	861-896	1.000000	78.3
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.256054	44618	45395	858-893	1.000000	78.1
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	44618	45395	860-895	1.000000	77.9
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.320736	44618	45395	879-914	1.000000	77.6
trnS-GGA-rps4_p1	trnS-GGA-rps4	1	AGGCTTTTGTTTACCGAGGG	ACCCTTCACCCATTCCCATA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.099964	59669	60023	509-509	1.000000	79.8
trnS-GGA-rps4_p2	trnS-GGA-rps4	2	CCGAGTGGTTTAAGGCGTAG	ACCCTTCACCCATTCCCATA	20	20	55.0	50.0	58.4	58.0	0.0	0.0	0.368613	59669	60023	546-546	1.000000	77.6
trnS-GGA-rps4_p3	trnS-GGA-rps4	3	GGCTTTTGTTTACCGAGGGT	ACCCTTCACCCATTCCCATA	20	20	50.0	50.0	58.4	58.0	0.0	0.0	0.392925	59669	60023	508-508	1.000000	77.5
trnS-GGA-rps4_p4	trnS-GGA-rps4	4	AGGCTTTTGTTTACCGAGGG	TTTGACCCTTCACCCATTCC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.396950	59669	60023	513-513	1.000000	77.4
trnS-GGA-rps4_p5	trnS-GGA-rps4	5	GAGATGGCCGAGTGGTTTAA	ACCCTTCACCCATTCCCATA	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.402191	59669	60023	553-553	1.000000	77.4
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	AGCCAATTAAGTCCGTAGCG	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.196808	61114	61993	953-954	1.000000	78.8
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GAGTCGAACCGATGACCATC	AGCCAATTAAGTCCGTAGCG	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.222618	61114	61993	984-985	1.000000	78.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	61114	61993	941-942	1.000000	77.4
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GAGTCGAACCGATGACCATC	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	58.2	58.2	0.0	10.2	0.406999	61114	61993	972-973	1.000000	77.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	GCCAATTAAGTCCGTAGCGT	20	20	55.0	50.0	57.9	58.4	0.0	0.0	0.484236	61114	61993	952-953	1.000000	76.5
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	58.0	58.1	10.3	0.0	0.089643	63127	64100	1111-1116	1.000000	80.0
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	GAGTAGATGTTGCGCCAGAA	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.0	58.1	4.2	0.0	0.097283	63127	64100	1143-1148	1.000000	79.9
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	AGTAGATGTTGCGCCAGAAG	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.0	58.1	4.2	0.0	0.102874	63127	64100	1142-1147	1.000000	79.9
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.139034	63127	64100	1075-1080	1.000000	79.6
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	ATGAGTAGATGTTGCGCCAG	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.1	58.1	4.2	0.0	0.141751	63127	64100	1145-1150	1.000000	79.6
trnV-UAC_p1	trnV-UAC	1	AACAAACAGACCGAAGAGCT	ACTTTTGTCAAGTAGTCGGT	20	20	45.0	40.0	57.4	54.6	0.0	0.0	4.063391	71332	72006	812-813	1.000000	48.0
trnV-UAC_p2	trnV-UAC	2	CAAACAAACAGACCGAAGAGC	ACTTTTGTCAAGTAGTCGGT	21	20	47.6	40.0	58.0	54.6	0.0	0.0	4.454281	71332	72006	814-815	1.000000	44.9
trnV-UAC_p3	trnV-UAC	3	AAACAAACAGACCGAAGAGCT	ACTTTTGTCAAGTAGTCGGT	21	20	42.9	40.0	58.1	54.6	0.0	0.0	4.522099	71332	72006	813-814	1.000000	44.3
trnV-UAC_p4	trnV-UAC	4	AAACAAACAGACCGAAGAGC	ACTTTTGTCAAGTAGTCGGT	20	20	45.0	40.0	56.6	54.6	0.0	0.0	4.862356	71332	72006	813-814	1.000000	41.6
trnV-UAC_p5	trnV-UAC	5	ACAAACAGACCGAAGAGCTA	ACTTTTGTCAAGTAGTCGGT	20	20	45.0	40.0	56.5	54.6	0.0	0.0	4.932388	71332	72006	811-812	1.000000	41.0
accD_p1	accD	1	CGCGGCTTCTAGTGATTCTT	CACCTACTACGGCTCCCATA	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.080984	75115	75234	710-785	1.000000	81.0
accD_p2	accD	2	ACGAGTTGAGAGAGGCTCTT	TTTTCGGTTGTTTGTGCACC	20	20	50.0	45.0	58.1	58.0	19.8	15.7	0.084459	75115	75234	862-937	1.000000	80.6
accD_p3	accD	3	CGCGGCTTCTAGTGATTCTT	TTTTCGGTTGTTTGTGCACC	20	20	50.0	45.0	58.1	58.0	0.0	15.7	0.074436	75115	75234	1053-1128	1.000000	80.5
accD_p4	accD	4	GATCGGGATGAGGATTGTCG	CACCTACTACGGCTCCCATA	20	20	55.0	55.0	57.9	58.0	0.0	0.0	0.080567	75115	75234	1018-1093	1.000000	80.5
accD_p5	accD	5	GATCGGGATGAGGATTGTCG	TTTTCGGTTGTTTGTGCACC	20	20	55.0	45.0	57.9	58.0	0.0	15.7	0.074019	75115	75234	1361-1436	1.000000	80.3
accD-psaI_p1	accD-psaI	1	GGTGCACAAACAACCGAAAA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	58.0	58.0	20.4	26.9	0.029334	75863	76519	832-834	1.000000	80.1
accD-psaI_p2	accD-psaI	2	GGTGCACAAACAACCGAAAA	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	58.0	57.9	20.4	26.8	0.105868	75863	76519	838-840	1.000000	79.5
accD-psaI_p3	accD-psaI	3	TCGATCCAGTCTTACCACGT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.2	58.0	0.0	26.9	0.196724	75863	76519	792-794	1.000000	78.8
accD-psaI_p4	accD-psaI	4	TGAGTTACTTGAGCTGCACG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.2	58.0	1.0	26.9	0.243719	75863	76519	749-751	1.000000	78.5
accD-psaI_p5	accD-psaI	5	TCGATCCAGTCTTACCACGT	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.2	57.9	0.0	26.8	0.273258	75863	76519	798-800	1.000000	78.2
ycf4-cemA_p1	ycf4-cemA	1	CATTCCCTTGACCCGTACTG	AGAGAGATCCACCAGGGTAA	20	20	55.0	50.0	58.0	56.7	0.0	0.0	1.274782	77484	78465	1152-1153	1.000000	70.1
ycf4-cemA_p2	ycf4-cemA	2	CATTCCCTTGACCCGTACTG	TGATAGAGAGATCCACCAGGG	20	21	55.0	52.4	58.0	57.7	0.0	0.0	1.275192	77484	78465	1156-1157	1.000000	70.1
ycf4-cemA_p3	ycf4-cemA	3	CATTCCCTTGACCCGTACTG	GATAGAGAGATCCACCAGGGT	20	21	55.0	52.4	58.0	57.7	0.0	0.0	1.276729	77484	78465	1155-1156	1.000000	70.1
ycf4-cemA_p4	ycf4-cemA	4	ATTCCCTTGACCCGTACTGA	AGAGAGATCCACCAGGGTAA	20	20	50.0	50.0	58.1	56.7	0.0	0.0	1.303622	77484	78465	1151-1152	1.000000	69.8
ycf4-cemA_p5	ycf4-cemA	5	TTCCCTTGACCCGTACTGAT	AGAGAGATCCACCAGGGTAA	20	20	50.0	50.0	58.1	56.7	0.0	0.0	1.303622	77484	78465	1150-1151	1.000000	69.8
petA-psbJ_p1	petA-psbJ	1	CGAGAAGGTTCAATTGTCCG	ACTGGCATTCTTGTGATCGT	20	20	50.0	45.0	56.8	57.5	5.1	0.0	1.642059	80384	81477	1157-1203	1.000000	70.5
petA-psbJ_p2	petA-psbJ	2	CGAGAAGGTTCAATTGTCCG	CGTATTCTGGATTGGGCTCA	20	20	50.0	50.0	56.8	57.4	5.1	0.0	1.782744	80384	81477	1111-1157	1.000000	69.5
petA-psbJ_p3	petA-psbJ	3	CGAGAAGGTTCAATTGTCCGA	ACTGGCATTCTTGTGATCGT	21	20	47.6	45.0	58.3	57.5	5.1	0.0	1.795598	80384	81477	1157-1203	1.000000	69.3
petA-psbJ_p4	petA-psbJ	4	TCGAGAAGGTTCAATTGTCCG	ACTGGCATTCTTGTGATCGT	21	20	47.6	45.0	58.3	57.5	5.1	0.0	1.795598	80384	81477	1158-1204	1.000000	69.3
petA-psbJ_p5	petA-psbJ	5	CGAGAAGGTTCAATTGTCCG	ATTCTGGATTGGGCTCATCC	20	20	50.0	50.0	56.8	57.3	5.1	7.8	1.834389	80384	81477	1108-1154	1.000000	69.1
psbA-trnH-GUG_p1	psbA-trnH-GUG	1	CTAGACCTAGCCGCTATCGA	TTTTGTAGTTGGTGGGCGAA	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.150452	95929	96327	529-529	1.000000	79.6
psbA-trnH-GUG_p2	psbA-trnH-GUG	2	TCTAGACCTAGCCGCTATCG	TTTTGTAGTTGGTGGGCGAA	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.150452	95929	96327	530-530	1.000000	79.6
psbA-trnH-GUG_p3	psbA-trnH-GUG	3	CTAGACCTAGCCGCTATCGA	TCACAATCCACTGCCTTGAC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.200000	95929	96327	479-479	1.000000	79.2
psbA-trnH-GUG_p4	psbA-trnH-GUG	4	TCTAGACCTAGCCGCTATCG	TCACAATCCACTGCCTTGAC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.200000	95929	96327	480-480	1.000000	79.2
psbA-trnH-GUG_p5	psbA-trnH-GUG	5	CCGTGCTAACCTTGGTATGG	TTTTGTAGTTGGTGGGCGAA	20	20	55.0	45.0	58.3	57.9	0.0	0.0	0.390538	95929	96327	584-584	1.000000	77.6
rps19_p1	rps19	1	TTCGCCCACCAACTACAAAA	TGGCTCGGCATGTTCTATTC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.100673	96418	96447	130-130	0.500000	59.2
rps19_p2	rps19	2	GTCAAGGCAGTGGATTGTGA	TGGCTCGGCATGTTCTATTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.150221	96418	96447	180-180	0.500000	58.8
rps19_p3	rps19	3	TTCGCCCACCAACTACAAAA	AAGAAGATTTGGCTCGGCAT	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.189021	96418	96447	139-139	0.500000	58.5
rps19_p4	rps19	4	TTCGCCCACCAACTACAAAA	AGAAGAACCCGACTCGGTAT	20	20	45.0	50.0	57.9	57.9	0.0	16.3	0.195071	96418	96447	98-98	0.500000	58.4
rps19_p5	rps19	5	TTCGCCCACCAACTACAAAA	GCTCGGCATGTTCTATTCGA	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.201290	96418	96447	128-128	0.500000	58.4
ndhE-psaC_p1	ndhE-psaC	1	TGAAGCAGCTATTGGACTGG	TCATAAATCTTTACTGAATGTGACA	20	25	50.0	28.0	57.6	54.8	0.0	0.0	8.622891	119428	119745	430-435	1.000000	41.7
ndhE-psaC_p2	ndhE-psaC	2	AGCAGCTATTGGACTGGCTA	TCATAAATCTTTACTGAATGTGACA	20	25	50.0	28.0	58.5	54.8	0.0	0.0	8.714145	119428	119745	427-432	1.000000	41.7
ndhE-psaC_p3	ndhE-psaC	3	GCAGCTATTGGACTGGCTAT	TCATAAATCTTTACTGAATGTGACA	20	25	50.0	28.0	57.4	54.8	0.0	0.0	8.772539	119428	119745	426-431	1.000000	41.7
ndhE-psaC_p4	ndhE-psaC	4	TGAAGCAGCTATTGGACTGG	CATAAATCTTTACTGAATGTGACA	20	24	50.0	29.2	57.6	53.4	0.0	0.0	9.001818	119428	119745	429-434	1.000000	41.7
ndhE-psaC_p5	ndhE-psaC	5	AGCAGCTATTGGACTGGCTA	CATAAATCTTTACTGAATGTGACA	20	24	50.0	29.2	58.5	53.4	0.0	0.0	9.093072	119428	119745	426-431	1.000000	41.7
ccsA-trnL-UAG_p1	ccsA-trnL-UAG	1	TGGAAACCATTGAAAAGGAA	CTCTTAGGAAGCAGTGCGAG	20	20	35.0	55.0	53.1	58.1	0.0	10.0	4.946417	122818	122932	223-223	1.000000	41.9
ccsA-trnL-UAG_p2	ccsA-trnL-UAG	2	TGGAAACCATTGAAAAGGAA	CTTAGGAAGCAGTGCGAGAG	20	20	35.0	55.0	53.1	58.1	0.0	10.0	4.946417	122818	122932	221-221	1.000000	41.9
ccsA-trnL-UAG_p3	ccsA-trnL-UAG	3	TGGAAACCATTGAAAAGGAA	TAGACACGCTGCTCTTAGGA	20	20	35.0	50.0	53.1	57.9	0.0	0.0	4.990612	122818	122932	234-234	1.000000	41.4
ccsA-trnL-UAG_p4	ccsA-trnL-UAG	4	TGGAAACCATTGAAAAGGAAA	CTCTTAGGAAGCAGTGCGAG	21	20	33.3	55.0	54.1	58.1	0.0	10.0	5.025571	122818	122932	223-223	1.000000	41.4
ccsA-trnL-UAG_p5	ccsA-trnL-UAG	5	TGGAAACCATTGAAAAGGAAA	CTTAGGAAGCAGTGCGAGAG	21	20	33.3	55.0	54.1	58.1	0.0	10.0	5.025571	122818	122932	221-221	1.000000	41.4
trnL-UAG-ndhG_p1	trnL-UAG-ndhG	1	CTCGCACTGCTTCCTAAGAG	TAGGTGCAATTTCTGTGGCT	20	20	55.0	45.0	58.1	57.4	0.0	0.0	0.664917	123013	123264	331-331	0.500000	54.7
trnL-UAG-ndhG_p2	trnL-UAG-ndhG	2	CTCTCGCACTGCTTCCTAAG	TAGGTGCAATTTCTGTGGCT	20	20	55.0	45.0	58.1	57.4	0.0	0.0	0.664917	123013	123264	333-333	0.500000	54.7
trnL-UAG-ndhG_p3	trnL-UAG-ndhG	3	TCCTAAGAGCAGCGTGTCTA	TAGGTGCAATTTCTGTGGCT	20	20	50.0	45.0	57.9	57.4	0.0	0.0	0.709112	123013	123264	320-320	0.500000	54.3
trnL-UAG-ndhG_p4	trnL-UAG-ndhG	4	AGCAGCGTGTCTACCAATTT	TAGGTGCAATTTCTGTGGCT	20	20	45.0	45.0	57.8	57.4	0.0	0.0	0.769839	123013	123264	313-313	0.500000	53.8
trnL-UAG-ndhG_p5	trnL-UAG-ndhG	5	CTAAGAGCAGCGTGTCTACC	TAGGTGCAATTTCTGTGGCT	20	20	55.0	45.0	57.8	57.4	0.0	0.0	0.781130	123013	123264	318-318	0.500000	53.8