primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	TTGGGTATGGCTCCTTCAAC	GTGCTTGGGAGTCCTTGAAA	20	20	50.0	50.0	57.5	58.0	0.0	9.6	0.526150	82	1143	1152-1152	1.000000	75.9
psbA_p2	psbA	2	GGTATGGCTCCTTCAACGAT	GTGCTTGGGAGTCCTTGAAA	20	20	50.0	50.0	57.4	58.0	0.0	9.6	0.640807	82	1143	1149-1149	1.000000	74.9
psbA_p3	psbA	3	TGGCTCCTTCAACGATTCTT	GTGCTTGGGAGTCCTTGAAA	20	20	45.0	50.0	57.1	58.0	0.0	9.6	0.877909	82	1143	1145-1145	1.000000	73.0
psbA_p4	psbA	4	TTGGGTATGGCTCCTTCAAC	TAGTACGTGTGCTTGGGAGT	20	20	50.0	50.0	57.5	58.4	0.0	0.0	0.883071	82	1143	1160-1160	1.000000	73.0
psbA_p5	psbA	5	TTGGGTATGGCTCCTTCAAC	GTTAGTACGTGTGCTTGGGA	20	20	50.0	50.0	57.5	57.6	0.0	0.0	0.951294	82	1143	1162-1162	1.000000	72.5
rpoC2_p1	rpoC2	1	TCCGTTGGACATAGATCCCA	ACCCGGATCTTCGTCCATAA	20	20	50.0	50.0	57.8	58.2	0.0	0.7	0.384100	18891	19040	278-278	1.000000	77.5
rpoC2_p2	rpoC2	2	TCCGTTGGACATAGATCCCA	TTACCCGGATCTTCGTCCAT	20	20	50.0	50.0	57.8	58.2	0.0	0.7	0.384100	18891	19040	280-280	1.000000	77.5
rpoC2_p3	rpoC2	3	CGTTCCTGGCGGTATCAAAA	ACCCGGATCTTCGTCCATAA	20	20	50.0	50.0	58.6	58.2	0.0	0.7	0.770806	18891	19040	220-220	1.000000	74.6
rpoC2_p4	rpoC2	4	CGTTCCTGGCGGTATCAAAA	TTACCCGGATCTTCGTCCAT	20	20	50.0	50.0	58.6	58.2	0.0	0.7	0.770806	18891	19040	222-222	1.000000	74.6
rpoC2_p5	rpoC2	5	TCCGTTGGACATAGATCCCA	TCTTACCCGGATCTTCGTCC	20	20	50.0	55.0	57.8	58.6	0.0	0.7	0.771806	18891	19040	282-282	1.000000	74.4
psbM-trnE-UUC_p1	psbM-trnE-UUC	1	AGAATGAACAGTGCAGTAGC	CGAATCCCCGTTTTCTCCTT	20	20	45.0	50.0	55.8	57.9	0.0	0.0	2.338723	30570	31811	1346-1347	1.000000	61.6
psbM-trnE-UUC_p2	psbM-trnE-UUC	2	AGAATGAACAGTGCAGTAGC	TTTCGTAGTACCCTACCCCC	20	20	45.0	55.0	55.8	57.8	0.0	0.0	2.388478	30570	31811	1375-1376	1.000000	61.2
psbM-trnE-UUC_p3	psbM-trnE-UUC	3	AGAATGAACAGTGCAGTAGC	GGAAGTCGAATCCCCGTTTT	20	20	45.0	50.0	55.8	58.2	0.0	7.2	2.411941	30570	31811	1352-1353	1.000000	61.0
psbM-trnE-UUC_p4	psbM-trnE-UUC	4	AGAATGAACAGTGCAGTAGC	TGTCCTGAACCACTAGACGA	20	20	45.0	50.0	55.8	57.7	0.0	0.0	2.510837	30570	31811	1316-1317	1.000000	60.2
psbM-trnE-UUC_p5	psbM-trnE-UUC	5	AGTGCGAGAATATTGACTTCCA	CGAATCCCCGTTTTCTCCTT	22	20	40.9	50.0	57.5	57.9	0.0	0.0	2.581329	30570	31811	1322-1323	1.000000	59.7
trnD-GUC-trnT-GGU_p1	trnD-GUC-trnT-GGU	1	CCTCGCTGGGATTGTAGTTC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.064646	32399	32727	464-465	1.000000	80.5
trnD-GUC-trnT-GGU_p2	trnD-GUC-trnT-GGU	2	CCTCGCTGGGATTGTAGTTC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.122742	32399	32727	443-444	1.000000	80.1
trnD-GUC-trnT-GGU_p3	trnD-GUC-trnT-GGU	3	CCTCGCTGGGATTGTAGTTC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.144897	32399	32727	440-441	1.000000	79.9
trnD-GUC-trnT-GGU_p4	trnD-GUC-trnT-GGU	4	TACACCTCGCTGGGATTGTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	58.1	58.0	10.4	0.0	0.150675	32399	32727	468-469	1.000000	79.8
trnD-GUC-trnT-GGU_p5	trnD-GUC-trnT-GGU	5	TACACCTCGCTGGGATTGTA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	58.1	58.1	10.4	0.0	0.208772	32399	32727	447-448	1.000000	79.4
rbcL_p1	rbcL	1	TGAGTTGTAGGGAGGGACTT	ACTGAATTATGCGCGCTTAG	20	20	50.0	45.0	57.3	56.7	0.0	33.3	2.022594	56964	58406	1486-1486	1.000000	63.8
rbcL_p2	rbcL	2	TGAGTTGTAGGGAGGGACTT	CTGAATTATGCGCGCTTAGT	20	20	50.0	45.0	57.3	56.7	0.0	33.3	2.022594	56964	58406	1485-1485	1.000000	63.8
rbcL_p3	rbcL	3	TGAGTTGTAGGGAGGGACTT	ACTGAATTATGCGCGCTTAGT	20	21	50.0	42.9	57.3	58.4	0.0	33.3	2.164785	56964	58406	1486-1486	1.000000	62.7
rbcL_p4	rbcL	4	TCATGAGTTGTAGGGAGGGA	ACTGAATTATGCGCGCTTAG	20	20	50.0	45.0	57.1	56.7	0.0	33.3	2.247345	56964	58406	1489-1489	1.000000	62.0
rbcL_p5	rbcL	5	TCATGAGTTGTAGGGAGGGA	CTGAATTATGCGCGCTTAGT	20	20	50.0	45.0	57.1	56.7	0.0	33.3	2.247345	56964	58406	1488-1488	1.000000	62.0
rbcL-accD_p1	rbcL-accD	1	GCAAATGGAGCCCTGAACTA	CACCAGTATGTCCAACAGCA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.312249	58407	59134	938-940	1.000000	78.2
rbcL-accD_p2	rbcL-accD	2	CGCTGCTTGTGAAGTATGGA	CACCAGTATGTCCAACAGCA	20	20	50.0	50.0	58.3	57.8	0.0	0.0	0.457838	58407	59134	916-918	1.000000	77.0
rbcL-accD_p3	rbcL-accD	3	AAATGGAGCCCTGAACTAGC	CACCAGTATGTCCAACAGCA	20	20	50.0	50.0	57.6	57.8	0.0	0.0	0.610321	58407	59134	936-938	1.000000	75.8
rbcL-accD_p4	rbcL-accD	4	GCAAATGGAGCCCTGAACTA	CCACCAGTATGTCCAACAGC	20	20	50.0	55.0	57.9	58.5	0.0	0.0	0.675616	58407	59134	939-941	1.000000	75.3
rbcL-accD_p5	rbcL-accD	5	CGCTGCTTGTGAAGTATGGA	CCACCAGTATGTCCAACAGC	20	20	50.0	55.0	58.3	58.5	0.0	0.0	0.821205	58407	59134	917-919	1.000000	74.1
ycf4-cemA_p1	ycf4-cemA	1	TGCGCGTACCAATTGAAGTA	ATGAGAGAGAGACCCACCAG	20	20	45.0	55.0	57.9	57.8	8.4	0.0	0.239365	62063	62916	959-959	1.000000	78.3
ycf4-cemA_p2	ycf4-cemA	2	CTTGCGCGTACCAATTGAAG	ATGAGAGAGAGACCCACCAG	20	20	50.0	55.0	58.1	57.8	8.4	0.0	0.275442	62063	62916	961-961	1.000000	78.0
ycf4-cemA_p3	ycf4-cemA	3	TGCGCGTACCAATTGAAGTA	GGGAGGGAAGTCAATGCTTT	20	20	45.0	50.0	57.9	57.8	8.4	0.0	0.303952	62063	62916	912-912	1.000000	77.8
ycf4-cemA_p4	ycf4-cemA	4	CTTGCGCGTACCAATTGAAG	GGGAGGGAAGTCAATGCTTT	20	20	50.0	50.0	58.1	57.8	8.4	0.0	0.340029	62063	62916	914-914	1.000000	77.5
ycf4-cemA_p5	ycf4-cemA	5	GGAACCATTCCCTTGACTCG	ATGAGAGAGAGACCCACCAG	20	20	55.0	55.0	58.3	57.8	0.0	0.0	0.418573	62063	62916	1041-1041	1.000000	76.9
rpl20-rps12_p1	rpl20-rps12	1	TTCTGTTGAGTAGCGGTTCG	CAGCGTAGGGGAACATGTAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.124393	70974	71741	913-916	1.000000	79.4
rpl20-rps12_p2	rpl20-rps12	2	TTCTGTTGAGTAGCGGTTCG	AATATCACGAAATCCCCCGC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.168116	70974	71741	949-952	1.000000	79.1
rpl20-rps12_p3	rpl20-rps12	3	GGTTCGGGTAAGCCTTGAAT	CAGCGTAGGGGAACATGTAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.188345	70974	71741	899-902	1.000000	78.9
rpl20-rps12_p4	rpl20-rps12	4	TTCTGTTGAGTAGCGGTTCG	AGCGTAGGGGAACATGTACT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.198344	70974	71741	912-915	1.000000	78.9
rpl20-rps12_p5	rpl20-rps12	5	TTCTGTTGAGTAGCGGTTCG	TCAGCGTAGGGGAACATGTA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.205031	70974	71741	914-917	1.000000	78.8
petB_p1	petB	1	TCTGGTAGTTCGACTGTGGA	ACTATTAGTCCTTCCCGATATGA	20	23	50.0	39.1	57.7	55.8	0.0	0.0	5.473367	76953	78410	1584-1586	1.000000	40.1
petB_p2	petB	2	TCTGGTAGTTCGACTGTGGA	TGAAATACTATTAGTCCTTCCCGA	20	24	50.0	37.5	57.7	56.7	0.0	0.0	5.578232	76953	78410	1590-1592	1.000000	40.1
petB_p3	petB	3	TCTGGTAGTTCGACTGTGGA	AGTCCTTCCCGATATGATAA	20	20	50.0	40.0	57.7	52.6	0.0	0.0	5.705135	76953	78410	1578-1580	1.000000	40.1
petB_p4	petB	4	TCTGGTAGTTCGACTGTGGA	ATTAGTCCTTCCCGATATGA	20	20	50.0	40.0	57.7	52.6	0.0	0.0	5.705135	76953	78410	1581-1583	1.000000	40.1
petB_p5	petB	5	TCTGGTAGTTCGACTGTGGA	TTAGTCCTTCCCGATATGAT	20	20	50.0	40.0	57.7	52.6	0.0	0.0	5.705135	76953	78410	1580-1582	1.000000	40.1
rpoA_p1	rpoA	1	ATACGGATCGCGTGTTACAG	ACATAATGGATGTCGACCTCC	20	21	50.0	47.6	57.9	57.2	16.4	27.4	1.868642	80034	81053	1203-1204	1.000000	65.2
rpoA_p2	rpoA	2	ATACGGATCGCGTGTTACAG	TGATGTAACACCTATGCCACA	20	21	50.0	42.9	57.9	57.0	16.4	0.0	2.106306	80034	81053	1221-1222	1.000000	63.3
rpoA_p3	rpoA	3	CGGATCGCGTGTTACAGATA	ACATAATGGATGTCGACCTCC	20	21	50.0	47.6	57.6	57.2	5.8	27.4	2.147840	80034	81053	1200-1201	1.000000	63.0
rpoA_p4	rpoA	4	ATACGGATCGCGTGTTACAG	CATAATGGATGTCGACCTCCT	20	21	50.0	47.6	57.9	57.0	16.4	31.6	2.152929	80034	81053	1202-1203	1.000000	62.9
rpoA_p5	rpoA	5	ATACGGATCGCGTGTTACAG	TGGATGTCGACCTCCTAAAA	20	20	50.0	45.0	57.9	55.9	16.4	31.6	2.241652	80034	81053	1197-1198	1.000000	62.2
ycf2_p1	ycf2	1	TGATTGAACAACCGGGATCA	AGAAGGGGATAGGGCTAAGC	20	20	45.0	55.0	57.1	58.3	6.3	0.0	1.133175	93304	93423	221-222	1.000000	73.1
ycf2_p2	ycf2	2	TGATTGAACAACCGGGATCA	AACAGGATCGTCCAGTTCCT	20	20	45.0	50.0	57.1	58.4	6.3	8.3	1.223392	93304	93423	267-268	1.000000	72.0
ycf2_p3	ycf2	3	TGATTGAACAACCGGGATCA	AAACAGGATCGTCCAGTTCC	20	20	45.0	50.0	57.1	57.5	6.3	12.7	1.343511	93304	93423	268-269	1.000000	71.0
ycf2_p4	ycf2	4	TGATTGAACAACCGGGATCAA	AGAAGGGGATAGGGCTAAGC	21	20	42.9	55.0	57.9	58.3	6.3	0.0	1.411627	93304	93423	221-222	1.000000	70.9
ycf2_p5	ycf2	5	TGATTGAACAACCGGGATCA	ACAGGATCGTCCAGTTCCTA	20	20	45.0	50.0	57.1	57.5	6.3	6.8	1.395989	93304	93423	266-267	1.000000	70.6
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	TGGCAACTTTACGTAAGGCA	CGGGGGAGCAAGTCAAAATA	20	20	45.0	50.0	57.7	57.9	16.5	0.0	0.380474	101036	102660	1781-1782	1.000000	77.0
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	TGGCAACTTTACGTAAGGCA	GATGACTTCCACCACGTCAA	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.421509	101036	102660	1720-1721	1.000000	76.7
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	TGGCAACTTTACGTAAGGCA	AACTGATGACTTCCACCACG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	101036	102660	1724-1725	1.000000	76.7
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	TGGCAACTTTACGTAAGGCA	ATGACTTCCACCACGTCAAG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	101036	102660	1719-1720	1.000000	76.7
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	TGGCAACTTTACGTAAGGCA	CTGATGACTTCCACCACGTC	20	20	45.0	55.0	57.7	58.3	16.5	0.0	0.542896	101036	102660	1722-1723	1.000000	75.7
ycf1_p1	ycf1	1	TGGTATCATTGACATAAGAGATGTC	AATATATGGGAGGTGGGCGA	25	20	36.0	50.0	56.5	58.0	10.1	0.0	6.540180	111558	112474	1125-1135	1.000000	40.8
ycf1_p2	ycf1	2	TGGTATCATTGACATAAGAGATGT	AATATATGGGAGGTGGGCGA	24	20	33.3	50.0	55.4	58.0	0.0	0.0	6.622132	111558	112474	1125-1135	1.000000	40.8
ycf1_p3	ycf1	3	TGGTATCATTGACATAAGAGATGTC	ATATATGGGAGGTGGGCGAG	25	20	36.0	55.0	56.5	58.4	10.1	0.0	6.928210	111558	112474	1124-1134	1.000000	40.8
ycf1_p4	ycf1	4	TGGTATCATTGACATAAGAGATGT	ATATATGGGAGGTGGGCGAG	24	20	33.3	55.0	55.4	58.4	0.0	0.0	7.010161	111558	112474	1124-1134	1.000000	40.8
ycf1_p5	ycf1	5	GGTATCATTGACATAAGAGATGTC	AATATATGGGAGGTGGGCGA	24	20	37.5	50.0	54.9	58.0	10.1	0.0	7.107135	111558	112474	1124-1134	1.000000	40.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGCAAAGTCAGTTTCTACCGG	AGCGTGTCTACCGATTTCAC	21	20	47.6	50.0	58.2	58.0	0.0	0.0	1.240326	115855	116490	740-743	1.000000	71.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGCAAAGTCAGTTTCTACCGG	CAGCGTGTCTACCGATTTCA	21	20	47.6	50.0	58.2	58.0	0.0	0.0	1.241696	115855	116490	741-744	1.000000	71.6
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GCAAAGTCAGTTTCTACCGGA	AGCGTGTCTACCGATTTCAC	21	20	47.6	50.0	58.2	58.0	9.5	0.0	1.245085	115855	116490	739-742	1.000000	71.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GCAAAGTCAGTTTCTACCGGA	CAGCGTGTCTACCGATTTCA	21	20	47.6	50.0	58.2	58.0	9.5	0.0	1.246456	115855	116490	740-743	1.000000	71.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GCAAAGTCAGTTTCTACCGG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	56.7	58.0	0.0	0.0	1.260753	115855	116490	739-742	1.000000	71.4
ccsA_p1	ccsA	1	GAGTGGCGGCATACTCTAAA	AGGGTTCTCAAAAACTCGCA	20	20	50.0	45.0	57.4	57.7	17.2	0.0	0.938750	116649	117617	1116-1116	1.000000	72.5
ccsA_p2	ccsA	2	GAGTGGCGGCATACTCTAAA	GGGTTCTCAAAAACTCGCAC	20	20	50.0	50.0	57.4	57.6	17.2	0.0	0.997920	116649	117617	1115-1115	1.000000	72.0
ccsA_p3	ccsA	3	AGTGGCGGCATACTCTAAAA	AGGGTTCTCAAAAACTCGCA	20	20	45.0	45.0	56.9	57.7	17.2	0.0	1.413784	116649	117617	1115-1115	1.000000	68.7
ccsA_p4	ccsA	4	AGTGGCGGCATACTCTAAAAG	AGGGTTCTCAAAAACTCGCA	21	20	47.6	45.0	58.1	57.7	17.2	0.0	1.435492	116649	117617	1115-1115	1.000000	68.5
ccsA_p5	ccsA	5	GTGGCGGCATACTCTAAAAGA	AGGGTTCTCAAAAACTCGCA	21	20	47.6	45.0	58.1	57.7	17.2	0.0	1.440598	116649	117617	1114-1114	1.000000	68.5
ccsA-ndhD_p1	ccsA-ndhD	1	ATTCGGCACTTGTAGCTTCT	TTTCGTTCTCTCGCTGTCAG	20	20	45.0	50.0	57.2	57.9	0.0	0.0	0.822042	117618	117845	407-409	1.000000	76.0
ccsA-ndhD_p2	ccsA-ndhD	2	ACAAATTCGGCACTTGTAGC	TTTCGTTCTCTCGCTGTCAG	20	20	45.0	50.0	57.0	57.9	0.0	0.0	1.041301	117618	117845	411-413	1.000000	74.2
ccsA-ndhD_p3	ccsA-ndhD	3	AATTCGGCACTTGTAGCTTCT	TTTCGTTCTCTCGCTGTCAG	21	20	42.9	50.0	57.9	57.9	0.0	0.0	1.111230	117618	117845	408-410	1.000000	73.7
ccsA-ndhD_p4	ccsA-ndhD	4	ATTCGGCACTTGTAGCTTCT	CGATTTCGTTCTCTCGCTGT	20	20	45.0	50.0	57.2	58.4	0.0	0.0	1.151303	117618	117845	410-412	1.000000	73.3
ccsA-ndhD_p5	ccsA-ndhD	5	ATTCGGCACTTGTAGCTTCT	TTCTCTCGCTGTCAGTTGAC	20	20	45.0	50.0	57.2	57.6	0.0	5.3	1.194089	117618	117845	402-404	1.000000	73.0
ndhG_p1	ndhG	1	TGGTTGACTAGAATCGAGCA	ACCCTTATTTTTCCTGGACT	20	20	45.0	40.0	56.3	53.8	0.0	0.0	5.932187	120607	121137	716-716	1.000000	40.0
ndhG_p2	ndhG	2	TGGTTGACTAGAATCGAGCAA	ACCCTTATTTTTCCTGGACT	21	20	42.9	40.0	57.0	53.8	0.0	0.0	6.176430	120607	121137	716-716	1.000000	40.0
ndhG_p3	ndhG	3	TTGGTTGACTAGAATCGAGCA	ACCCTTATTTTTCCTGGACT	21	20	42.9	40.0	57.0	53.8	0.0	0.0	6.176430	120607	121137	717-717	1.000000	40.0
ndhG_p4	ndhG	4	TTGGTTGACTAGAATCGAGCAA	ACCCTTATTTTTCCTGGACT	22	20	40.9	40.0	57.7	53.8	9.9	0.0	6.488622	120607	121137	717-717	1.000000	40.0
ndhG_p5	ndhG	5	TTTGGTTGACTAGAATCGAGCA	ACCCTTATTTTTCCTGGACT	22	20	40.9	40.0	57.7	53.8	0.0	0.0	6.488622	120607	121137	718-718	1.000000	40.0
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	CGGGGGAGCAAGTCAAAATA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.9	57.7	0.0	16.5	0.380474	140522	142146	1781-1782	1.000000	77.0
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	GATGACTTCCACCACGTCAA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.421509	140522	142146	1720-1721	1.000000	76.7
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	AACTGATGACTTCCACCACG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	140522	142146	1724-1725	1.000000	76.7
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	ATGACTTCCACCACGTCAAG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	140522	142146	1719-1720	1.000000	76.7
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	CTGATGACTTCCACCACGTC	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.3	57.7	0.0	16.5	0.542896	140522	142146	1722-1723	1.000000	75.7