primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-matK_p1	trnH-GUG-matK	1	TTGCCTTCATCCATTTGGCT	TCTGGCTTCAAAAGGAACGT	20	20	45.0	45.0	58.1	57.7	0.0	0.0	0.400469	75	609	1236-1388	1.000000	85.9
trnH-GUG-matK_p2	trnH-GUG-matK	2	TTGCCTTCATCCATTTGGCT	TGGCTTCAAAAGGAACGTCT	20	20	45.0	45.0	58.1	57.7	0.0	0.0	0.400469	75	609	1234-1386	1.000000	85.9
trnH-GUG-matK_p3	trnH-GUG-matK	3	TTGCCTTCATCCATTTGGCT	TGGGGCATTGACTAAAGCAA	20	20	45.0	45.0	58.1	57.7	0.0	0.0	0.359578	75	609	972-972	0.500000	57.1
trnH-GUG-matK_p4	trnH-GUG-matK	4	TTGCCTTCATCCATTTGGCT	TTGGGGCATTGACTAAAGCA	20	20	45.0	45.0	58.1	57.7	0.0	0.0	0.359578	75	609	973-973	0.500000	57.1
trnH-GUG-matK_p5	trnH-GUG-matK	5	TTGCCTTCATCCATTTGGCT	AGCGAACCTTTGGTTGGTTA	20	20	45.0	45.0	58.1	57.6	0.0	6.7	0.410329	75	609	1299-1299	0.500000	56.7
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CTCTTTTCCTTCTCGTCGGG	CGTAGGTTCGAATCCTTCCG	20	20	55.0	55.0	58.0	58.2	0.0	19.8	0.165947	2831	3869	1126-1208	1.000000	88.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CTCTTTTCCTTCTCGTCGGG	TGGCTAAGTGGTAAGGCAAC	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.196532	2831	3869	1165-1247	1.000000	88.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CTCTTTTCCTTCTCGTCGGG	CTAAGTGGTAAGGCAACGGG	20	20	55.0	55.0	58.0	58.3	0.0	0.0	0.268168	2831	3869	1162-1244	1.000000	87.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AGCTCTTTTCCTTCTCGTCG	CGTAGGTTCGAATCCTTCCG	20	20	50.0	55.0	57.6	58.2	0.0	19.8	0.519333	2831	3869	1128-1210	1.000000	85.8
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TCTTTTCCTTCTCGTCGGGA	CGTAGGTTCGAATCCTTCCG	20	20	50.0	55.0	58.4	58.2	0.0	19.8	0.539280	2831	3869	1125-1207	1.000000	85.7
atpA_p1	atpA	1	ACGGAAAGAGAGGGATTCGA	AGTTGTTTTACCGCCGAGTT	20	20	50.0	45.0	58.2	58.0	0.0	0.0	0.193680	4598	5941	1966-1966	0.500000	58.5
atpA_p2	atpA	2	GTTGCCTTACCACTTAGCCA	AGTTGTTTTACCGCCGAGTT	20	20	50.0	45.0	57.8	58.0	0.0	0.0	0.236533	4598	5941	2158-2158	0.500000	58.1
atpA_p3	atpA	3	TTGCCTTACCACTTAGCCAC	AGTTGTTTTACCGCCGAGTT	20	20	50.0	45.0	57.8	58.0	0.0	0.0	0.236533	4598	5941	2157-2157	0.500000	58.1
atpA_p4	atpA	4	CCCGTTGCCTTACCACTTAG	AGTTGTTTTACCGCCGAGTT	20	20	55.0	45.0	58.3	58.0	0.0	0.0	0.308169	4598	5941	2161-2161	0.500000	57.5
atpA_p5	atpA	5	AAAAGATATGCCCTGGGACG	AGTTGTTTTACCGCCGAGTT	20	20	50.0	45.0	57.7	58.0	19.3	0.0	0.390742	4598	5941	2215-2215	0.500000	56.9
atpF-atpH_p1	atpF-atpH	1	AACTCGGCGGTAAAACAACT	GAGACAACTCGAGGCTGAAG	20	20	45.0	55.0	58.0	58.0	7.2	18.4	0.052901	6434	6746	824-824	0.500000	59.6
atpF-atpH_p2	atpF-atpH	2	AACTCGGCGGTAAAACAACT	TGACCCTGACCTTGCAGATA	20	20	45.0	50.0	58.0	58.0	7.2	0.0	0.091122	6434	6746	1217-1217	0.500000	59.3
atpF-atpH_p3	atpF-atpH	3	AACTCGGCGGTAAAACAACT	TGTAGGGCTTGCTTCTTTGG	20	20	45.0	50.0	58.0	58.1	7.2	0.0	0.132632	6434	6746	887-887	0.500000	58.9
atpF-atpH_p4	atpF-atpH	4	AACTCGGCGGTAAAACAACT	ATTTCTGCTGCTTCTGTGCT	20	20	45.0	45.0	58.0	58.1	7.2	0.0	0.135342	6434	6746	922-922	0.500000	58.9
atpF-atpH_p5	atpF-atpH	5	AACTCGGCGGTAAAACAACT	CAACTCGAGGCTGAAGGAAA	20	20	45.0	50.0	58.0	57.8	7.2	18.4	0.212164	6434	6746	820-820	0.500000	58.3
trnY-GUA-trnE-UUC_p1	trnY-GUA-trnE-UUC	1	ACGAATTTACAGTCCGTCCC	CAAGGAGACAGCGAGGATTC	20	20	50.0	55.0	57.6	58.1	0.0	0.0	0.441926	9468	9530	141-156	1.000000	80.8
trnY-GUA-trnE-UUC_p2	trnY-GUA-trnE-UUC	2	CAGTCCGTCCCTATTAACCG	CAAGGAGACAGCGAGGATTC	20	20	55.0	55.0	57.5	58.1	0.0	0.0	0.570673	9468	9530	132-147	1.000000	80.0
trnY-GUA-trnE-UUC_p3	trnY-GUA-trnE-UUC	3	CTATTAACCGCTCGGGCATC	CAAGGAGACAGCGAGGATTC	20	20	55.0	55.0	58.6	58.1	8.6	0.0	0.637291	9468	9530	122-137	1.000000	79.8
trnY-GUA-trnE-UUC_p4	trnY-GUA-trnE-UUC	4	ACGAATTTACAGTCCGTCCC	GAGGATTCGAATTCCCCTGG	20	20	50.0	55.0	57.6	57.7	0.0	18.7	0.637596	9468	9530	129-144	1.000000	79.6
trnY-GUA-trnE-UUC_p5	trnY-GUA-trnE-UUC	5	GCCAACGAATTTACAGTCCG	CAAGGAGACAGCGAGGATTC	20	20	50.0	55.0	57.5	58.1	0.0	0.0	0.587440	9468	9530	145-160	1.000000	79.5
trnS-GGA-rps4_p1	trnS-GGA-rps4	1	ATGTAGGAGAGATGGCCGAG	AAATGAATTGCTAGTCGTAGA	20	21	55.0	33.3	58.4	52.7	0.0	0.0	6.663891	12330	12705	493-510	1.000000	52.0
trnS-GGA-rps4_p2	trnS-GGA-rps4	2	ATGTAGGAGAGATGGCCGAG	AATGAATTGCTAGTCGTAGAA	20	21	55.0	33.3	58.4	52.7	0.0	0.0	6.663891	12330	12705	492-509	1.000000	52.0
trnS-GGA-rps4_p3	trnS-GGA-rps4	3	ATGTAGGAGAGATGGCCGAG	TGCTAGTCGTAGAATATTATTCTCGT	20	26	55.0	34.6	58.4	57.7	0.0	19.7	6.693709	12330	12705	485-502	1.000000	52.0
trnS-GGA-rps4_p4	trnS-GGA-rps4	4	ATGTAGGAGAGATGGCCGAG	AAATGAATTGCTAGTCGTAGAA	20	22	55.0	31.8	58.4	53.6	0.0	0.0	6.779556	12330	12705	493-510	1.000000	52.0
trnS-GGA-rps4_p5	trnS-GGA-rps4	5	ATGTAGGAGAGATGGCCGAG	ATGAATTGCTAGTCGTAGAAT	20	21	55.0	33.3	58.4	52.5	0.0	0.0	6.898749	12330	12705	491-508	1.000000	52.0
trnL-UAA_p1	trnL-UAA	1	CGAAGCCATACCCAATCGAA	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	58.1	58.1	0.0	20.1	0.103791	13519	14046	1368-1439	1.000000	89.0
trnL-UAA_p2	trnL-UAA	2	AATGGGTGGAGGGTCAAATG	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.8	58.1	0.0	20.1	0.288385	13519	14046	1568-1639	1.000000	87.1
trnL-UAA_p3	trnL-UAA	3	TGTAATGGGTGGAGGGTCAA	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	58.3	58.1	0.0	20.1	0.303163	13519	14046	1571-1642	1.000000	87.0
trnL-UAA_p4	trnL-UAA	4	TTGTAATGGGTGGAGGGTCA	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	58.3	58.1	0.0	20.1	0.303163	13519	14046	1572-1643	1.000000	86.9
trnL-UAA_p5	trnL-UAA	5	CGAAGCCATACCCAATCGAA	ACCAGCTGAGCTATCCTGAT	20	20	50.0	50.0	58.1	57.9	0.0	17.4	0.152023	13519	14046	1400-1400	0.500000	58.8
atpE_p1	atpE	1	CGGCAAAAGTCATTGGTTCA	ACCTAGAATCGTAGGGGAGC	20	20	45.0	55.0	57.2	58.0	0.0	9.5	0.793161	15276	15656	942-942	0.500000	53.7
atpE_p2	atpE	2	CGGCAAAAGTCATTGGTTCA	CCTAGAATCGTAGGGGAGCA	20	20	45.0	55.0	57.2	58.0	0.0	0.0	0.794585	15276	15656	941-941	0.500000	53.6
atpE_p3	atpE	3	ACGGCAAAAGTCATTGGTTC	ACCTAGAATCGTAGGGGAGC	20	20	45.0	55.0	57.2	58.0	0.0	9.5	0.795055	15276	15656	943-943	0.500000	53.6
atpE_p4	atpE	4	ACGGCAAAAGTCATTGGTTC	CCTAGAATCGTAGGGGAGCA	20	20	45.0	55.0	57.2	58.0	0.0	0.0	0.796479	15276	15656	942-942	0.500000	53.6
atpE_p5	atpE	5	CGGCAAAAGTCATTGGTTCA	CTGCTCCTGCGACTACATTT	20	20	45.0	50.0	57.2	58.0	0.0	0.0	0.807119	15276	15656	1062-1062	0.500000	53.5
atpB_p1	atpB	1	CGCTGTAGCAATAGGAGCAT	CGACCTAATTGTACCACGCA	20	20	50.0	50.0	57.8	58.0	4.6	0.0	0.161774	15666	17065	1728-1728	0.500000	58.7
atpB_p2	atpB	2	CGCTGTAGCAATAGGAGCAT	ACTGAGGCAGTTCAAACAGG	20	20	50.0	50.0	57.8	58.0	4.6	0.0	0.187950	15666	17065	2189-2189	0.500000	58.5
atpB_p3	atpB	3	CGCTGTAGCAATAGGAGCAT	CTGAAGGTTCACAAGTGGCT	20	20	50.0	50.0	57.8	58.0	4.6	0.0	0.187950	15666	17065	1774-1774	0.500000	58.5
atpB_p4	atpB	4	CGCTGTAGCAATAGGAGCAT	GAAGGTTCACAAGTGGCTGA	20	20	50.0	50.0	57.8	58.0	4.6	0.0	0.193504	15666	17065	1772-1772	0.500000	58.5
atpB_p5	atpB	5	CGCTGTAGCAATAGGAGCAT	TTCCGGTAGCAATTGGAGTG	20	20	50.0	50.0	57.8	57.9	4.6	7.7	0.268772	15666	17065	2149-2149	0.500000	57.8
rps12-clpP_p1	rps12-clpP	1	ATAAGTTGTTTAATAGTTGGCAT	TGGAATTGTTGATCTTGTAGCA	23	22	26.1	36.4	52.4	56.0	0.0	0.0	12.609322	21364	21482	173-194	1.000000	51.0
rps12-clpP_p2	rps12-clpP	2	AATAAGTTGTTTAATAGTTGGCA	TGGAATTGTTGATCTTGTAGCA	23	22	26.1	36.4	52.6	56.0	0.0	0.0	12.391866	21364	21482	174-195	1.000000	50.9
rps12-clpP_p3	rps12-clpP	3	AATAAGTTGTTTAATAGTTGGCA	TGGAATTGTTGATCTTGTAGCAA	23	23	26.1	34.8	52.6	56.7	0.0	0.0	12.682163	21364	21482	174-195	1.000000	50.9
rps12-clpP_p4	rps12-clpP	4	AATAAGTTGTTTAATAGTTGGCAT	TGGAATTGTTGATCTTGTAGCA	24	22	25.0	36.4	53.2	56.0	0.0	0.0	12.772967	21364	21482	174-195	1.000000	50.9
rps12-clpP_p5	rps12-clpP	5	TCTAATAAGTTGTTTAATAGTTGGCA	TGGAATTGTTGATCTTGTAGCA	26	22	26.9	36.4	55.2	56.0	0.0	0.0	12.789365	21364	21482	177-198	1.000000	50.8
rps11-rpl36_p1	rps11-rpl36	1	TCAGTTTTTCGTGAATTAGTTCT	AGGCATAAACAAAGACAGGGA	23	21	30.4	42.9	54.5	57.2	0.0	0.0	8.271980	23795	23878	149-153	1.000000	50.1
rps11-rpl36_p2	rps11-rpl36	2	TCAGTTTTTCGTGAATTAGTTCTT	AGGCATAAACAAAGACAGGGA	24	21	29.2	42.9	55.3	57.2	0.0	0.0	8.537063	23795	23878	149-153	1.000000	50.1
rps11-rpl36_p3	rps11-rpl36	3	TCAGTTTTTCGTGAATTAGTTCT	AAGGCATAAACAAAGACAGGGA	23	22	30.4	40.9	54.5	57.9	0.0	0.0	8.577665	23795	23878	150-154	1.000000	50.0
rps11-rpl36_p4	rps11-rpl36	4	TCAGTTTTTCGTGAATTAGTTCT	CAAGGCATAAACAAAGACAGGG	23	22	30.4	45.5	54.5	57.8	0.0	0.0	8.643576	23795	23878	151-155	1.000000	49.9
rps11-rpl36_p5	rps11-rpl36	5	TCAGTTTTTCGTGAATTAGTTCT	CCAAGGCATAAACAAAGACAGG	23	22	30.4	45.5	54.5	57.8	0.0	0.0	8.643576	23795	23878	152-156	1.000000	49.9
rrn16-rrn23_p1	rrn16-rrn23	1	CAAGGTAGCCGTACTGGAAG	TCGATTACTACGCCCTTCCT	20	20	55.0	50.0	57.7	57.9	7.8	0.0	0.358223	43950	45351	1466-1495	1.000000	84.4
rrn16-rrn23_p2	rrn16-rrn23	2	CAAGGTAGCCGTACTGGAAG	GTCTCTGGGTGCCTAGGTAT	20	20	55.0	55.0	57.7	57.9	7.8	11.3	0.384853	43950	45351	1445-1474	1.000000	84.3
rrn16-rrn23_p3	rrn16-rrn23	3	AGTGACTGGAGTGAAGTCGT	TCGATTACTACGCCCTTCCT	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.369344	43950	45351	1488-1517	1.000000	84.2
rrn16-rrn23_p4	rrn16-rrn23	4	AGTGACTGGAGTGAAGTCGT	GTCTCTGGGTGCCTAGGTAT	20	20	50.0	55.0	58.3	57.9	0.0	11.3	0.395974	43950	45351	1467-1496	1.000000	84.1
rrn16-rrn23_p5	rrn16-rrn23	5	AAGTCGTAACAAGGTAGCCG	TCGATTACTACGCCCTTCCT	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.421910	43950	45351	1475-1504	1.000000	83.8