primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
tRNAK-rps16_p1	tRNAK-rps16	1	TGAGGATCAGTCGTGGTCTT	TCGAGCCGTATGAGGAGAAA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.316333	3945	4513	991-993	1.000000	79.3
tRNAK-rps16_p2	tRNAK-rps16	2	TGAGGATCAGTCGTGGTCTT	TTTCTCGAGCCGTATGAGGA	20	20	50.0	50.0	58.1	58.2	0.0	7.8	0.316333	3945	4513	995-997	1.000000	79.3
tRNAK-rps16_p3	tRNAK-rps16	3	ACGTCTAATTAGAGCGGGGA	TCGAGCCGTATGAGGAGAAA	20	20	50.0	50.0	57.9	58.2	26.5	0.0	0.304034	3945	4513	1172-1174	1.000000	79.2
tRNAK-rps16_p4	tRNAK-rps16	4	ACGTCTAATTAGAGCGGGGA	TTTCTCGAGCCGTATGAGGA	20	20	50.0	50.0	57.9	58.2	26.5	7.8	0.304034	3945	4513	1176-1178	1.000000	79.2
tRNAK-rps16_p5	tRNAK-rps16	5	TAGGAAGTTCAGCTCGTTGC	TCGAGCCGTATGAGGAGAAA	20	20	50.0	50.0	57.9	58.2	5.0	0.0	0.321350	3945	4513	1141-1143	1.000000	79.1
psbK-psbI_p1	psbK-psbI	1	TGGCAAGCTGCTGTAAGTTT	ACAAAAAGTTTGAGAGTAAGCA	20	22	45.0	31.8	58.3	54.4	1.4	7.7	5.872906	6914	7281	444-455	1.000000	45.7
psbK-psbI_p2	psbK-psbI	2	GTTTGGCAAGCTGCTGTAAG	ACAAAAAGTTTGAGAGTAAGCA	20	22	50.0	31.8	57.9	54.4	1.4	7.7	5.620197	6914	7281	447-458	1.000000	45.6
psbK-psbI_p3	psbK-psbI	3	GTTTGGCAAGCTGCTGTAAG	AACAAAAAGTTTGAGAGTAAGCA	20	23	50.0	30.4	57.9	55.2	1.4	7.7	5.846914	6914	7281	448-459	1.000000	45.6
psbK-psbI_p4	psbK-psbI	4	TGCCAAATTACCCGAAGCTT	ACAAAAAGTTTGAGAGTAAGCA	20	22	45.0	31.8	58.1	54.4	0.0	7.7	5.646230	6914	7281	535-546	1.000000	44.7
psbK-psbI_p5	psbK-psbI	5	TTGCCAAATTACCCGAAGCT	ACAAAAAGTTTGAGAGTAAGCA	20	22	45.0	31.8	58.1	54.4	0.0	7.7	5.646230	6914	7281	536-547	1.000000	44.7
LK95_t006-psbD_p1	LK95_t006-psbD	1	ATTAGCAATCCGCCGCTTTA	GAGCAATAGGCCAGACCATC	20	20	45.0	55.0	58.0	58.1	0.0	5.0	0.148662	7626	8663	1175-1211	1.000000	81.6
LK95_t006-psbD_p2	LK95_t006-psbD	2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	20	20	45.0	45.0	58.0	58.2	0.0	0.0	0.284276	7626	8663	1153-1189	1.000000	80.6
LK95_t006-psbD_p3	LK95_t006-psbD	3	ATTAGCAATCCGCCGCTTTA	CCCTTCGTAACCAGTCATCC	20	20	45.0	55.0	58.0	57.7	0.0	0.0	0.349331	7626	8663	1137-1173	1.000000	80.1
LK95_t006-psbD_p4	LK95_t006-psbD	4	GAGCGGAAAGAGAGGGATTC	GAGCAATAGGCCAGACCATC	20	20	55.0	55.0	57.8	58.1	0.0	5.0	0.343212	7626	8663	1226-1262	1.000000	80.0
LK95_t006-psbD_p5	LK95_t006-psbD	5	ATTAGCAATCCGCCGCTTTA	AGGCCAGACCATCCTACAAA	20	20	45.0	50.0	58.0	58.3	0.0	5.0	0.369381	7626	8663	1168-1204	1.000000	79.9
LK95_t012-LK95_t014_p1	LK95_t012-LK95_t014	1	CCTCAAGGTTATGAGCCTCG	CGACTTACGTCTTAGCACGA	20	20	55.0	50.0	57.8	57.5	0.0	10.2	0.746856	12761	14725	1578-2157	1.000000	78.6
LK95_t012-LK95_t014_p2	LK95_t012-LK95_t014	2	CCTCGTGAGCTACCAAACTG	CGACTTACGTCTTAGCACGA	20	20	55.0	50.0	58.3	57.5	0.0	10.2	0.800847	12761	14725	1563-2142	1.000000	78.2
LK95_t012-LK95_t014_p3	LK95_t012-LK95_t014	3	CAAGGTTATGAGCCTCGTGA	CGACTTACGTCTTAGCACGA	20	20	50.0	50.0	57.3	57.5	0.0	10.2	1.188603	12761	14725	1575-2154	1.000000	75.1
LK95_t012-LK95_t014_p4	LK95_t012-LK95_t014	4	TCAAGGTTATGAGCCTCGTG	CGACTTACGTCTTAGCACGA	20	20	50.0	50.0	57.3	57.5	0.0	10.2	1.188603	12761	14725	1576-2155	1.000000	75.1
LK95_t012-LK95_t014_p5	LK95_t012-LK95_t014	5	TATGAGCCTCGTGAGCTACC	CGACTTACGTCTTAGCACGA	20	20	55.0	50.0	58.7	57.5	0.0	10.2	1.196100	12761	14725	1569-2148	1.000000	75.0
LK95_t020-psbM_p1	LK95_t020-psbM	1	TTCAATTGGTCAGAGCACCG	AGAATGAACAGTGCAGTAGC	20	20	50.0	45.0	58.5	55.8	5.4	0.0	2.706630	15819	16633	923-926	1.000000	59.6
LK95_t020-psbM_p2	LK95_t020-psbM	2	GTTCAATTGGTCAGAGCACC	AGAATGAACAGTGCAGTAGC	20	20	50.0	45.0	57.0	55.8	5.4	0.0	3.226784	15819	16633	924-927	1.000000	55.4
LK95_t020-psbM_p3	LK95_t020-psbM	3	CGCGGGGATTGTAGTTCAA	AGAATGAACAGTGCAGTAGC	19	20	52.6	45.0	57.9	55.8	0.0	0.0	3.376082	15819	16633	937-940	1.000000	54.3
LK95_t020-psbM_p4	LK95_t020-psbM	4	TTCAATTGGTCAGAGCACCG	AGGAACTAGAATGAACAGTGCA	20	22	50.0	40.9	58.5	57.4	5.4	0.0	3.101264	15819	16633	930-933	0.923077	53.2
LK95_t020-psbM_p5	LK95_t020-psbM	5	TTCAATTGGTCAGAGCACCG	GGAACTAGAATGAACAGTGCAG	20	22	50.0	45.5	58.5	57.1	5.4	0.0	3.413216	15819	16633	929-932	0.923077	50.7
psbM-petN_p1	psbM-petN	1	TGCTACTGCACTGTTCATTCT	AGTATGGGGAAGGAGTGGAC	21	20	42.9	55.0	57.6	58.1	0.0	0.0	1.526483	16739	17370	731-753	1.000000	69.6
psbM-petN_p2	psbM-petN	2	TGCTACTGCACTGTTCATTCT	GTATGGGGAAGGAGTGGACT	21	20	42.9	55.0	57.6	58.1	0.0	0.0	1.526483	16739	17370	730-752	1.000000	69.6
psbM-petN_p3	psbM-petN	3	TGCTACTGCACTGTTCATTCT	TAGTAAGTCTCGCTTGGGCT	21	20	42.9	50.0	57.6	58.2	0.0	0.0	1.558772	16739	17370	789-811	1.000000	69.2
psbM-petN_p4	psbM-petN	4	TGCTACTGCACTGTTCATTCT	TATGGGGAAGGAGTGGACTC	21	20	42.9	55.0	57.6	57.8	0.0	0.0	1.583671	16739	17370	729-751	1.000000	69.2
psbM-petN_p5	psbM-petN	5	TGCTACTGCACTGTTCATTCT	GGGGAAGGAGTGGACTCTAG	21	20	42.9	60.0	57.6	58.2	0.0	6.7	1.624352	16739	17370	726-748	1.000000	68.9
LK95_t030-LK95_t032_p1	LK95_t030-LK95_t032	1	ATTTGAACCGATGACCCTCG	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	58.0	58.1	0.0	20.1	0.076071	46141	47768	1747-1788	1.000000	80.5
LK95_t030-LK95_t032_p2	LK95_t030-LK95_t032	2	CGATGACCCTCGCATTACAA	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	58.1	58.1	0.0	20.1	0.117307	46141	47768	1739-1780	1.000000	80.2
LK95_t030-LK95_t032_p3	LK95_t030-LK95_t032	3	GCGATGCTCTAACCTCTGAG	TTTGAACTGGTGACACGAGG	20	20	55.0	50.0	57.9	58.1	0.0	20.1	0.184636	46141	47768	1717-1758	1.000000	79.6
LK95_t030-LK95_t032_p4	LK95_t030-LK95_t032	4	ATTTGAACCGATGACCCTCG	CTGCCAGGAACCAGATTTGA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.229198	46141	47768	1762-1803	1.000000	79.3
LK95_t030-LK95_t032_p5	LK95_t030-LK95_t032	5	ATTTGAACCGATGACCCTCG	TCTGCCAGGAACCAGATTTG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.229198	46141	47768	1763-1804	1.000000	79.3
rbcL-rpl23_p1	rbcL-rpl23	1	CGCAGCTTGTGAAGTATGGA	AGAGTTCGACCCAATGCTTT	20	20	50.0	45.0	58.3	57.4	0.0	0.0	0.842982	56181	56491	485-496	0.769231	67.6
rbcL-rpl23_p2	rbcL-rpl23	2	AGTTCGAGCCGGTAGATACT	AGAGTTCGACCCAATGCTTT	20	20	50.0	45.0	57.7	57.4	1.9	0.0	0.913747	56181	56491	450-461	0.769231	67.3
rbcL-rpl23_p3	rbcL-rpl23	3	GGCGATCAAATTCGAGTTCG	AGAGTTCGACCCAATGCTTT	20	20	50.0	45.0	57.6	57.4	0.0	0.0	0.921880	56181	56491	464-475	0.769231	67.1
rbcL-rpl23_p4	rbcL-rpl23	4	TGGAAGGCGATCAAATTCGA	AGAGTTCGACCCAATGCTTT	20	20	45.0	45.0	57.6	57.4	0.0	0.0	0.963552	56181	56491	469-480	0.769231	66.8
rbcL-rpl23_p5	rbcL-rpl23	5	AGCTTGCAAATGGAGTCCTG	AGAGTTCGACCCAATGCTTT	20	20	50.0	45.0	58.5	57.4	11.3	0.0	1.026130	56181	56491	512-523	0.769231	66.0
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	AGGCGTATCTTGTTCAAGCC	20	20	45.0	50.0	58.0	58.3	0.0	7.6	0.278273	62808	63971	922-1375	1.000000	83.0
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	AGGCGTATCTTGTTCAAGCC	20	20	50.0	50.0	57.9	58.3	6.4	7.6	0.312268	62808	63971	911-1364	1.000000	82.7
psbE-petL_p3	psbE-petL	3	ACCAACCCGCAATGAATAGG	AGGCGTATCTTGTTCAAGCC	20	20	50.0	50.0	58.2	58.3	0.0	7.6	0.498226	62808	63971	913-1366	1.000000	81.3
psbE-petL_p4	psbE-petL	4	TGACAAATAACCAACCCGCA	GGCGTATCTTGTTCAAGCCA	20	20	45.0	50.0	58.0	58.6	0.0	7.6	0.568355	62808	63971	921-1374	1.000000	80.7
psbE-petL_p5	psbE-petL	5	CAACCCGCAATGAATAGGGA	GGCGTATCTTGTTCAAGCCA	20	20	50.0	50.0	57.9	58.6	6.4	7.6	0.602350	62808	63971	910-1363	1.000000	80.4
petG-LK95_t035_p1	petG-LK95_t035	1	GGGATTATTCGTGACTGCGT	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.1	58.0	0.0	2.6	0.080095	64361	64481	253-254	1.000000	82.8
petG-LK95_t035_p2	petG-LK95_t035	2	GGGATTATTCGTGACTGCGT	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	58.1	57.9	0.0	1.4	0.153017	64361	64481	217-218	1.000000	82.8
petG-LK95_t035_p3	petG-LK95_t035	3	AGCGGGATTATTCGTGACTG	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	57.8	58.0	0.0	2.6	0.241096	64361	64481	256-257	1.000000	81.5
petG-LK95_t035_p4	petG-LK95_t035	4	GCGTATTTGCAATACAGGCG	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.3	58.0	0.0	2.6	0.274035	64361	64481	237-238	1.000000	81.5
petG-LK95_t035_p5	petG-LK95_t035	5	AGCGGGATTATTCGTGACTG	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	57.8	57.9	0.0	1.4	0.314018	64361	64481	220-221	1.000000	81.5
psbT-psbN_p1	psbT-psbN	1	TCTTCTTCCGAGAACCACCT	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026579	71110	71157	217-217	1.000000	82.4
psbT-psbN_p2	psbT-psbN	2	TTCCGAGAACCACCTAAGGT	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035264	71110	71157	212-212	1.000000	82.4
psbT-psbN_p3	psbT-psbN	3	AAGGTTCCACCAACTCCAAC	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.082822	71110	71157	197-197	1.000000	82.2
psbT-psbN_p4	psbT-psbN	4	CCACCTAAGGTTCCACCAAC	TCGCCATCTCCATATCTGGT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.109819	71110	71157	203-203	1.000000	81.9
psbT-psbN_p5	psbT-psbN	5	TCTTCTTCCGAGAACCACCT	CCATTCGAAGAACACGGAGA	20	20	50.0	50.0	58.0	57.6	0.0	3.3	0.374910	71110	71157	128-128	1.000000	81.4
infA-rps8_p1	infA-rps8	1	TGGTGTCATTCTCTAGCCGA	GGGTGGAATGGGAATTGCTA	20	20	50.0	50.0	58.2	57.5	0.0	0.0	0.605272	76943	77115	308-325	1.000000	78.0
infA-rps8_p2	infA-rps8	2	AGCCTAGGATGATGGTGTCA	GGGTGGAATGGGAATTGCTA	20	20	50.0	50.0	57.8	57.5	14.8	0.0	0.633546	76943	77115	320-337	1.000000	77.7
infA-rps8_p3	infA-rps8	3	TTGACCCTATCCCCCATCAG	GGGTGGAATGGGAATTGCTA	20	20	55.0	50.0	58.2	57.5	0.0	0.0	0.632848	76943	77115	378-395	1.000000	77.2
infA-rps8_p4	infA-rps8	4	TTTGACCCTATCCCCCATCA	GGGTGGAATGGGAATTGCTA	20	20	50.0	50.0	57.7	57.5	0.0	0.0	0.747870	76943	77115	379-396	1.000000	76.3
infA-rps8_p5	infA-rps8	5	TGGTGTCATTCTCTAGCCGA	TTGGGTGGAATGGGAATTGC	20	20	50.0	50.0	58.2	58.7	0.0	0.0	0.877960	76943	77115	310-327	1.000000	75.8
ndhF-rpl32_p1	ndhF-rpl32	1	GGAAGTGGGAGAAGAGGGAT	TTGACATTGAAGTACGTTTTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	104833	105740	980-1024	1.000000	42.1
ndhF-rpl32_p2	ndhF-rpl32	2	GGAAGTGGGAGAAGAGGGAT	TTTGACATTGAAGTACGTTTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	104833	105740	981-1025	1.000000	42.1
ndhF-rpl32_p3	ndhF-rpl32	3	GGAAGTGGGAGAAGAGGGAT	TTTTGACATTGAAGTACGTTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	104833	105740	982-1026	1.000000	42.1
ndhF-rpl32_p4	ndhF-rpl32	4	GGAAGTGGGAGAAGAGGGAT	TTTTTGACATTGAAGTACGTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	104833	105740	983-1027	1.000000	42.1
ndhF-rpl32_p5	ndhF-rpl32	5	GGAAGTGGGAGAAGAGGGAT	TCCAAATATTTTTACGAATACGCT	20	24	55.0	29.2	58.1	55.3	0.0	0.0	6.833076	104833	105740	1007-1051	1.000000	42.0
rpl32-LK95_t054_p1	rpl32-LK95_t054	1	GAGCGGTAATGAGCATCCAA	TTCGAACCGAGATGCTTGAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.050149	105933	106646	845-871	1.000000	82.0
rpl32-LK95_t054_p2	rpl32-LK95_t054	2	TTTTCGAGCGGTAATGAGCA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.091209	105933	106646	850-876	1.000000	81.7
rpl32-LK95_t054_p3	rpl32-LK95_t054	3	GAGCGGTAATGAGCATCCAA	GGATTCGAACCGAGATGCTT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.105116	105933	106646	848-874	1.000000	81.6
rpl32-LK95_t054_p4	rpl32-LK95_t054	4	GAGCGGTAATGAGCATCCAA	GATCCGCACAAAAGAGTTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.077338	105933	106646	1391-1417	1.000000	80.9
rpl32-LK95_t054_p5	rpl32-LK95_t054	5	TTTTCGAGCGGTAATGAGCA	GATCCGCACAAAAGAGTTGC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.118398	105933	106646	1396-1422	1.000000	80.6
ccsA_p1	ccsA	1	CTCAAGCATCTCGGTTCGAA	TCTGGACCACGAGAACTCTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.006910	106798	107766	1370-1375	1.000000	81.3
ccsA_p2	ccsA	2	CTCAAGCATCTCGGTTCGAA	TTCTGGACCACGAGAACTCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.006910	106798	107766	1371-1376	1.000000	81.3
ccsA_p3	ccsA	3	CTCAAGCATCTCGGTTCGAA	GGATTCTGGACCACGAGAAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.015248	106798	107766	1374-1379	1.000000	81.3
ccsA_p4	ccsA	4	CTCAAGCATCTCGGTTCGAA	TGAGTGGTTTTGTCGCAGAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.034967	106798	107766	1567-1572	1.000000	80.9
ccsA_p5	ccsA	5	CTCAAGCATCTCGGTTCGAA	GCCTTACCAGGAATGAGTGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.047831	106798	107766	1580-1585	1.000000	80.8
ccsA-ndhD_p1	ccsA-ndhD	1	CGAATTCTGCACTTATAGCTTCG	TTGACAGGGTAGAGGCTCTC	23	20	43.5	55.0	58.0	58.1	3.5	22.5	3.146553	107767	107932	324-329	1.000000	59.7
ccsA-ndhD_p2	ccsA-ndhD	2	CGAATTCTGCACTTATAGCTTCG	GACAGGGTAGAGGCTCTCTT	23	20	43.5	55.0	58.0	57.8	3.5	22.5	3.153549	107767	107932	322-327	1.000000	59.7
ccsA-ndhD_p3	ccsA-ndhD	3	CGAATTCTGCACTTATAGCTTCG	CCCTATCCGTTGACAGGGTA	23	20	43.5	55.0	58.0	58.2	3.5	0.0	3.216157	107767	107932	333-338	1.000000	59.0
ccsA-ndhD_p4	ccsA-ndhD	4	CGAATTCTGCACTTATAGCTTCG	TCTCCCTATCCGTTGACAGG	23	20	43.5	55.0	58.0	58.2	3.5	0.0	3.223854	107767	107932	336-341	1.000000	58.9
ccsA-ndhD_p5	ccsA-ndhD	5	CGAATTCTGCACTTATAGCTTCG	GTTGACAGGGTAGAGGCTCT	23	20	43.5	55.0	58.0	58.4	3.5	22.5	3.437733	107767	107932	325-330	1.000000	57.4
psaC-ndhE_p1	psaC-ndhE	1	GCTTTACATCCATCCCAGGG	AGCCGCTATTGGACTATCCA	20	20	55.0	50.0	58.0	58.3	0.0	0.0	0.296086	109801	110277	656-692	1.000000	81.4
psaC-ndhE_p2	psaC-ndhE	2	GGCTTTACATCCATCCCAGG	AGCCGCTATTGGACTATCCA	20	20	55.0	50.0	58.0	58.3	0.0	0.0	0.296086	109801	110277	657-693	1.000000	81.4
psaC-ndhE_p3	psaC-ndhE	3	GTTGGACAAGCTCGTACACA	AGCCGCTATTGGACTATCCA	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.422292	109801	110277	617-653	1.000000	80.7
psaC-ndhE_p4	psaC-ndhE	4	TGTTGGACAAGCTCGTACAC	AGCCGCTATTGGACTATCCA	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.422292	109801	110277	618-654	1.000000	80.7
psaC-ndhE_p5	psaC-ndhE	5	CTGTTGGACAAGCTCGTACA	AGCCGCTATTGGACTATCCA	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.431884	109801	110277	619-655	1.000000	80.6