primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ycf1	1	ATGGTCTCCTGGTTAGCCTC	AACATACTAAAGCGGCTCCC	20	20	55.0	50.0	58.5	57.7	0.0	1.4	0.825618	110948	111067	607-607	0.500000	53.4
ycf1_p2	ycf1	2	GGTCTCCTGGTTAGCCTCAT	AACATACTAAAGCGGCTCCC	20	20	55.0	50.0	58.5	57.7	0.0	1.4	0.825618	110948	111067	605-605	0.500000	53.4
ycf1_p3	ycf1	3	ATGGTCTCCTGGTTAGCCTC	GGCTCCCATTATTGGTGTCA	20	20	55.0	50.0	58.5	57.6	0.0	3.1	0.933791	110948	111067	594-594	0.500000	52.5
ycf1_p4	ycf1	4	GGTCTCCTGGTTAGCCTCAT	GGCTCCCATTATTGGTGTCA	20	20	55.0	50.0	58.5	57.6	0.0	3.1	0.933791	110948	111067	592-592	0.500000	52.5
ycf1_p5	ycf1	5	GGTCTCCTGGTTAGCCTCAT	CATACTAAAGCGGCTCCCAT	20	20	55.0	50.0	58.5	57.4	0.0	1.4	1.049249	110948	111067	603-603	0.500000	51.6
ycf1-rps15_p1	ycf1-rps15	1	AATTATGGAACCTGCCCGTC	CCGCAGACCTCTCTGAGATA	20	20	50.0	55.0	57.9	58.0	0.0	8.0	0.078517	111448	111841	1064-1064	0.500000	59.4
ycf1-rps15_p2	ycf1-rps15	2	AATTATGGAACCTGCCCGTC	AACACCATGCATTGATGGGT	20	20	50.0	45.0	57.9	58.1	0.0	8.9	0.111405	111448	111841	1338-1338	0.500000	59.1
ycf1-rps15_p3	ycf1-rps15	3	AATTATGGAACCTGCCCGTC	TAAGCCAGTAACCGTTGACG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.121619	111448	111841	1099-1099	0.500000	59.0
ycf1-rps15_p4	ycf1-rps15	4	TATGGAACCTGCCCGTCTAT	CCGCAGACCTCTCTGAGATA	20	20	50.0	55.0	57.9	58.0	0.0	8.0	0.122845	111448	111841	1061-1061	0.500000	59.0
ycf1-rps15_p5	ycf1-rps15	5	TATGGAACCTGCCCGTCTAT	AACACCATGCATTGATGGGT	20	20	50.0	45.0	57.9	58.1	0.0	8.9	0.155733	111448	111841	1335-1335	0.500000	58.8
rps15-ndhH_p1	rps15-ndhH	1	CGTCAACGGTTACTGGCTTA	GTAAATCAGCAGCGACTCCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.078982	112121	112208	686-686	0.500000	59.4
rps15-ndhH_p2	rps15-ndhH	2	CGTCAACGGTTACTGGCTTA	TAAATCAGCAGCGACTCCAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.078982	112121	112208	685-685	0.500000	59.4
rps15-ndhH_p3	rps15-ndhH	3	CGTCAACGGTTACTGGCTTA	AACACCATGCATTGATGGGT	20	20	50.0	45.0	57.9	58.1	0.0	8.9	0.127460	112121	112208	259-259	0.500000	59.0
rps15-ndhH_p4	rps15-ndhH	4	CGTCAACGGTTACTGGCTTA	AGGAGTCTGTGCCCCAATAT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.169974	112121	112208	574-574	0.500000	58.6
rps15-ndhH_p5	rps15-ndhH	5	AGGTCTGCGGAAAATTCTGG	TAAATCAGCAGCGACTCCAC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.183760	112121	112208	710-710	0.500000	58.5
ndhH_p1	ndhH	1	CGTCAACGGTTACTGGCTTA	CGTTGTTGTATACCCGCAGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.069789	112209	113390	1549-1549	0.500000	59.4
ndhH_p2	ndhH	2	CGTCAACGGTTACTGGCTTA	AAGAATTCCCAAAGGCCCTG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.126061	112209	113390	1586-1586	0.500000	59.0
ndhH_p3	ndhH	3	CGTCAACGGTTACTGGCTTA	CCTGACATAAGAAGGCCGAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.127512	112209	113390	1813-1813	0.500000	59.0
ndhH_p4	ndhH	4	CGTCAACGGTTACTGGCTTA	GACATAAGAAGGCCGACAGG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.127512	112209	113390	1810-1810	0.500000	59.0
ndhH_p5	ndhH	5	CGTCAACGGTTACTGGCTTA	TGAAGAATTCCCAAAGGCCC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.131646	112209	113390	1588-1588	0.500000	58.9
ndhA_p1	ndhA	1	GTGGAGTCGCTGCTGATTTA	TGCAGCTCGTACTGTTTGTT	20	20	50.0	45.0	58.0	58.1	0.0	8.8	0.062041	113392	115537	2983-2983	0.500000	59.5
ndhA_p2	ndhA	2	GTGGAGTCGCTGCTGATTTA	TTGCAGCTCGTACTGTTTGT	20	20	50.0	45.0	58.0	58.1	0.0	8.8	0.062041	113392	115537	2984-2984	0.500000	59.5
ndhA_p3	ndhA	3	TGGAGTCGCTGCTGATTTAC	TGCAGCTCGTACTGTTTGTT	20	20	50.0	45.0	58.0	58.1	0.0	8.8	0.062041	113392	115537	2982-2982	0.500000	59.5
ndhA_p4	ndhA	4	TGGAGTCGCTGCTGATTTAC	TTGCAGCTCGTACTGTTTGT	20	20	50.0	45.0	58.0	58.1	0.0	8.8	0.062041	113392	115537	2983-2983	0.500000	59.5
ndhA_p5	ndhA	5	TCAACAGGCCCTAGAAGGAA	TGCAGCTCGTACTGTTTGTT	20	20	50.0	45.0	58.0	58.1	0.0	8.8	0.090759	113392	115537	2640-2640	0.500000	59.3
ndhI_p1	ndhI	1	AGCCGAGTGTGAACGATTAC	AAAAACAATTCTCGCCCCCT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.017512	115616	116116	1105-1105	0.500000	59.9
ndhI_p2	ndhI	2	TACATATCCGTTCCCGAGCT	AAAAACAATTCTCGCCCCCT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.018560	115616	116116	921-921	0.500000	59.9
ndhI_p3	ndhI	3	TTTGTGACCTTTGAGGAGCC	AAAAACAATTCTCGCCCCCT	20	20	50.0	45.0	58.0	58.0	0.1	0.0	0.028425	115616	116116	1311-1311	0.500000	59.8
ndhI_p4	ndhI	4	GACCTTTGAGGAGCCGTATG	AAAAACAATTCTCGCCCCCT	20	20	55.0	45.0	58.1	58.0	0.1	0.0	0.068356	115616	116116	1306-1306	0.500000	59.5
ndhI_p5	ndhI	5	GTACATATCCGTTCCCGAGC	AAAAACAATTCTCGCCCCCT	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.071421	115616	116116	922-922	0.500000	59.4
ndhI-ndhG_p1	ndhI-ndhG	1	AACAAACAGTACGAGCTGCA	GCAGCTACAAAAGGGGAGTT	20	20	45.0	50.0	58.1	58.1	1.0	0.0	0.140838	116117	116476	1003-1003	0.500000	58.9
ndhI-ndhG_p2	ndhI-ndhG	2	ACAAACAGTACGAGCTGCAA	GCAGCTACAAAAGGGGAGTT	20	20	45.0	50.0	58.1	58.1	1.0	0.0	0.140838	116117	116476	1002-1002	0.500000	58.9
ndhI-ndhG_p3	ndhI-ndhG	3	AACAAACAGTACGAGCTGCA	TTTCCCACGGTCCAAAGATG	20	20	45.0	50.0	58.1	58.1	1.0	0.0	0.147609	116117	116476	1114-1114	0.500000	58.8
ndhI-ndhG_p4	ndhI-ndhG	4	ACAAACAGTACGAGCTGCAA	CATTTCCCACGGTCCAAAGA	20	20	45.0	50.0	58.1	58.1	1.0	0.0	0.147609	116117	116476	1115-1115	0.500000	58.8
ndhI-ndhG_p5	ndhI-ndhG	5	ACAAACAGTACGAGCTGCAA	TTTCCCACGGTCCAAAGATG	20	20	45.0	50.0	58.1	58.1	1.0	0.0	0.147609	116117	116476	1113-1113	0.500000	58.8
ndhG_p1	ndhG	1	AGGGGGCGAGAATTGTTTTT	TGAATCGATGAGTTCCCCCA	20	20	45.0	50.0	58.0	58.4	0.0	0.0	0.430784	116477	117007	1488-1488	0.500000	56.6
ndhG_p2	ndhG	2	AGGGGGCGAGAATTGTTTTT	GAGTCCAATAGCGGCTTCAG	20	20	45.0	55.0	58.0	58.4	0.0	8.3	0.433326	116477	117007	1259-1259	0.500000	56.5
ndhG_p3	ndhG	3	AGGGGGCGAGAATTGTTTTT	AATAGCGAGTCCAATAGCGG	20	20	45.0	50.0	58.0	57.6	0.0	0.0	0.450221	116477	117007	1265-1265	0.500000	56.4
ndhG_p4	ndhG	4	TTGATCACATCCGAGCGTTT	TGAATCGATGAGTTCCCCCA	20	20	45.0	50.0	57.9	58.4	0.0	0.0	0.514409	116477	117007	1933-1933	0.500000	55.9
ndhG_p5	ndhG	5	TTGATCACATCCGAGCGTTT	GAGTCCAATAGCGGCTTCAG	20	20	45.0	55.0	57.9	58.4	0.0	8.3	0.516951	116477	117007	1704-1704	0.500000	55.9
ndhG-ndhE_p1	ndhG-ndhE	1	AGTCACTTCCGTGGTCTGTA	TCCTCTGTTCTTGGAGCAGA	20	20	50.0	50.0	58.0	58.0	0.0	12.1	0.024335	117008	117223	1140-1140	0.500000	59.8
ndhG-ndhE_p2	ndhG-ndhE	2	GTCACTTCCGTGGTCTGTAC	TCCTCTGTTCTTGGAGCAGA	20	20	55.0	50.0	57.9	58.0	0.0	12.1	0.077196	117008	117223	1139-1139	0.500000	59.4
ndhG-ndhE_p3	ndhG-ndhE	3	AACTCCCCTTTTGTAGCTGC	TCCTCTGTTCTTGGAGCAGA	20	20	50.0	50.0	58.1	58.0	0.0	12.1	0.104399	117008	117223	1274-1274	0.500000	59.2
ndhG-ndhE_p4	ndhG-ndhE	4	CATCTTTGGACCGTGGGAAA	TCCTCTGTTCTTGGAGCAGA	20	20	50.0	50.0	58.1	58.0	0.0	12.1	0.111169	117008	117223	1163-1163	0.500000	59.1
ndhG-ndhE_p5	ndhG-ndhE	5	TCTTTGGACCGTGGGAAATG	TCCTCTGTTCTTGGAGCAGA	20	20	50.0	50.0	58.1	58.0	0.0	12.1	0.111169	117008	117223	1161-1161	0.500000	59.1
ndhE-psaC_p1	ndhE-psaC	1	TAATCGGCGCAATTGCTGTA	TCCTCTGTTCTTGGAGCAGA	20	20	45.0	50.0	58.3	58.0	26.9	12.1	0.357634	117530	117769	934-934	0.500000	57.1
ndhE-psaC_p2	ndhE-psaC	2	CTGAAGCCGCTATTGGACTC	TCCTCTGTTCTTGGAGCAGA	20	20	55.0	50.0	58.4	58.0	0.0	12.1	0.439101	117530	117769	472-472	0.500000	56.5
ndhE-psaC_p3	ndhE-psaC	3	CCGCTATTGGACTCGCTATT	TCCTCTGTTCTTGGAGCAGA	20	20	50.0	50.0	57.6	58.0	0.0	12.1	0.455995	117530	117769	466-466	0.500000	56.4
ndhE-psaC_p4	ndhE-psaC	4	TAATCGGCGCAATTGCTGTA	ACATCCGTCCCAAGGTATCA	20	20	45.0	50.0	58.3	58.1	26.9	0.0	0.468048	117530	117769	896-896	0.500000	56.3
ndhE-psaC_p5	ndhE-psaC	5	TAATCGGCGCAATTGCTGTA	CAACCGACACAATCCTCTGT	20	20	45.0	50.0	58.3	57.8	26.9	0.0	0.512010	117530	117769	946-946	0.500000	55.9
ndhD_p1	ndhD	1	TCTGCTCCAAGAACAGAGGA	ATTCACGCGGTTCTCGTAAA	20	20	50.0	45.0	58.0	57.9	0.0	12.5	0.084293	118130	119632	1915-1915	0.500000	59.3
ndhD_p2	ndhD	2	TCTGCTCCAAGAACAGAGGA	TCAGGAGCAGTATGGGCTAA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.190142	118130	119632	2268-2268	0.500000	58.5
ndhD_p3	ndhD	3	TCTGCTCCAAGAACAGAGGA	TTCAGGAGCAGTATGGGCTA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.190142	118130	119632	2269-2269	0.500000	58.5
ndhD_p4	ndhD	4	TGATACCTTGGGACGGATGT	ATTCACGCGGTTCTCGTAAA	20	20	50.0	45.0	58.1	57.9	0.0	12.5	0.194707	118130	119632	1953-1953	0.500000	58.4
ndhD_p5	ndhD	5	TCTGCTCCAAGAACAGAGGA	GCGGTTCTCGTAAAGACCAA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.228444	118130	119632	1909-1909	0.500000	58.2
ccsA_p1	ccsA	1	GTTTTTGATTCTGGACCGCG	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044083	119906	120874	1581-1581	0.500000	59.6
ccsA_p2	ccsA	2	GTTTTTGATTCTGGACCGCG	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.045454	119906	120874	1580-1580	0.500000	59.6
ccsA_p3	ccsA	3	GTTTTTGATTCTGGACCGCG	AATTTTGGTGGTGGGGAGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.045935	119906	120874	2104-2104	0.500000	59.6
ccsA_p4	ccsA	4	TTTACGAGAACCGCGTGAAT	GTGAAATCGGTAGACACGCT	20	20	45.0	50.0	57.9	58.0	4.9	0.0	0.076667	119906	120874	1285-1285	0.500000	59.4
ccsA_p5	ccsA	5	TTTACGAGAACCGCGTGAAT	TGAAATCGGTAGACACGCTG	20	20	45.0	50.0	57.9	58.0	4.9	0.0	0.078038	119906	120874	1284-1284	0.500000	59.4
ccsA-trnL-UAG_p1	ccsA-trnL-UAG	1	GAAGTAGCAAAGCCTTGGGT	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.096773	120875	120998	539-539	0.500000	59.2
ccsA-trnL-UAG_p2	ccsA-trnL-UAG	2	GAAGTAGCAAAGCCTTGGGT	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.098143	120875	120998	538-538	0.500000	59.2
ccsA-trnL-UAG_p3	ccsA-trnL-UAG	3	GAAGTAGCAAAGCCTTGGGT	AATTTTGGTGGTGGGGAGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.098624	120875	120998	1062-1062	0.500000	59.2
ccsA-trnL-UAG_p4	ccsA-trnL-UAG	4	GAAGTAGCAAAGCCTTGGGT	TGGTGGTGGGGAGTCTTATT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.142689	120875	120998	1057-1057	0.500000	58.9
ccsA-trnL-UAG_p5	ccsA-trnL-UAG	5	GAAGTAGCAAAGCCTTGGGT	TTGGTGGTGGGGAGTCTTAT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.142689	120875	120998	1058-1058	0.500000	58.9
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AGCGTGTCTACCGATTTCAC	AAAGGAAAGGATATGGGGCG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.372803	121079	121819	874-874	0.500000	57.0
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CAGCGTGTCTACCGATTTCA	AAAGGAAAGGATATGGGGCG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.374173	121079	121819	875-875	0.500000	57.0
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AGCGTGTCTACCGATTTCAC	CGGCGTTAAAAGCTTTGTCA	20	20	50.0	45.0	58.0	57.6	0.0	13.5	0.400763	121079	121819	856-856	0.500000	56.8
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	CAGCGTGTCTACCGATTTCA	CGGCGTTAAAAGCTTTGTCA	20	20	50.0	45.0	58.0	57.6	0.0	13.5	0.402133	121079	121819	857-857	0.500000	56.8
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	GAAGTAGCAAAGCCTTGGGT	AAAGGAAAGGATATGGGGCG	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.453915	121079	121819	1393-1393	0.500000	56.4
rpl32-ndhF_p1	rpl32-ndhF	1	GACTCCCCACCACCAAAATT	GCAGATTTAGCAATCGCACC	20	20	50.0	50.0	58.0	57.9	0.0	8.0	0.120722	121985	122852	2005-2005	0.500000	59.0
rpl32-ndhF_p2	rpl32-ndhF	2	GACTCCCCACCACCAAAATT	AACAGGTCGTGTGAACCAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.126508	121985	122852	1790-1790	0.500000	59.0
rpl32-ndhF_p3	rpl32-ndhF	3	GACTCCCCACCACCAAAATT	ACAGGTCGTGTGAACCAAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.126508	121985	122852	1789-1789	0.500000	59.0
rpl32-ndhF_p4	rpl32-ndhF	4	AATAAGACTCCCCACCACCA	GCAGATTTAGCAATCGCACC	20	20	50.0	50.0	57.9	57.9	0.0	8.0	0.164787	121985	122852	2010-2010	0.500000	58.7
rpl32-ndhF_p5	rpl32-ndhF	5	ATAAGACTCCCCACCACCAA	GCAGATTTAGCAATCGCACC	20	20	50.0	50.0	57.9	57.9	0.0	8.0	0.164787	121985	122852	2009-2009	0.500000	58.7
ndhF_p1	ndhF	1	AGGTAAGATCCGGTGAATCG	GCCGAGAAGAAGGTATCAGC	20	20	50.0	55.0	56.8	58.1	0.0	0.0	1.320594	122853	125078	2708-2708	0.500000	49.4
ndhF_p2	ndhF	2	AGGTAAGATCCGGTGAATCG	CTAGCACTGGGTAGACCTCA	20	20	50.0	55.0	56.8	57.9	0.0	0.0	1.325374	122853	125078	2624-2624	0.500000	49.4
ndhF_p3	ndhF	3	AGGTAAGATCCGGTGAATCG	CGCCTCTTCATCTAGCACTG	20	20	50.0	55.0	56.8	58.1	0.0	0.0	1.329601	122853	125078	2635-2635	0.500000	49.4
ndhF_p4	ndhF	4	AGGTAAGATCCGGTGAATCG	TCGTTGTGGTCGGACTCTAT	20	20	50.0	50.0	56.8	58.2	0.0	8.2	1.349698	122853	125078	2812-2812	0.500000	49.2
ndhF_p5	ndhF	5	AGGTAAGATCCGGTGAATCG	ATCAATTCGGTCGTTGTGGT	20	20	50.0	45.0	56.8	57.8	0.0	0.0	1.358889	122853	125078	2822-2822	0.500000	49.1