primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CCGACGCTTTAATCCACTCA	ACCGAAAGACCCTTTAACTGT	20	21	50.0	42.9	58.0	57.1	0.0	0.0	1.856041	8039	8854	899-917	1.000000	70.9
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	ACGGAAAGAGAGGGATTCGA	ACCGAAAGACCCTTTAACTGT	20	21	50.0	42.9	58.2	57.1	0.0	0.0	2.002806	8039	8854	957-975	1.000000	69.4
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ACGCTTTAATCCACTCAGCC	ACCGAAAGACCCTTTAACTGT	20	21	50.0	42.9	58.3	57.1	0.0	0.0	2.112392	8039	8854	896-914	1.000000	68.8
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CGCTTTAATCCACTCAGCCA	ACCGAAAGACCCTTTAACTGT	20	21	50.0	42.9	58.3	57.1	0.0	0.0	2.113620	8039	8854	895-913	1.000000	68.8
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	TGGAAACGGAAAGAGAGGGA	ACCGAAAGACCCTTTAACTGT	20	21	50.0	42.9	58.3	57.1	0.0	0.0	2.126914	8039	8854	962-980	1.000000	68.3
ycf1_p1	ycf1	1	CTCGACCAAAATCCCCCAAA	ACATACTGAAACGACTGCCA	20	20	50.0	45.0	58.1	57.2	0.0	0.0	0.913813	114314	114433	1110-1110	0.500000	52.7
ycf1_p2	ycf1	2	TCGACCAAAATCCCCCAAAG	ACATACTGAAACGACTGCCA	20	20	50.0	45.0	58.1	57.2	0.0	0.0	0.913813	114314	114433	1109-1109	0.500000	52.7
ycf1_p3	ycf1	3	AAAGAACCCACAAGCCAAGT	ACATACTGAAACGACTGCCA	20	20	45.0	45.0	57.8	57.2	0.0	0.0	0.990864	114314	114433	841-841	0.500000	52.1
ycf1_p4	ycf1	4	GGGACGAGACTTTGAACCAA	ACATACTGAAACGACTGCCA	20	20	50.0	45.0	57.8	57.2	0.0	0.0	1.081529	114314	114433	666-666	0.500000	51.3
ycf1_p5	ycf1	5	GACCAAAATCCCCCAAAGGA	ACATACTGAAACGACTGCCA	20	20	50.0	45.0	57.7	57.2	0.0	0.0	1.150710	114314	114433	1107-1107	0.500000	50.8
ycf1-rps15_p1	ycf1-rps15	1	GGGACGAGACTTTGAACCAA	TCCCAACGCGTTACATAAGG	20	20	50.0	50.0	57.8	58.0	0.0	26.1	0.262780	114778	115162	1680-1680	0.500000	57.9
ycf1-rps15_p2	ycf1-rps15	2	GGGACGAGACTTTGAACCAA	AACACCATGCATTGATGGGT	20	20	50.0	45.0	57.8	58.1	0.0	8.9	0.308466	114778	115162	1543-1543	0.500000	57.5
ycf1-rps15_p3	ycf1-rps15	3	GGGACGAGACTTTGAACCAA	TGCATTGATGGGTGATGAGG	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.311353	114778	115162	1536-1536	0.500000	57.5
ycf1-rps15_p4	ycf1-rps15	4	GGGACGAGACTTTGAACCAA	GCATTGATGGGTGATGAGGT	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.312814	114778	115162	1535-1535	0.500000	57.5
ycf1-rps15_p5	ycf1-rps15	5	GGGACGAGACTTTGAACCAA	CCCAGAATTTTCCGCAAACC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.328692	114778	115162	1271-1271	0.500000	57.4
ndhH_p1	ndhH	1	GGTTTGCGGAAAATTCTGGG	TCACTGCTAAGGATGGACCA	20	20	50.0	50.0	57.9	58.0	2.8	0.0	0.127639	115533	116714	1719-1719	0.500000	59.0
ndhH_p2	ndhH	2	GGTTTGCGGAAAATTCTGGG	TGCTAAGGATGGACCAATGC	20	20	50.0	50.0	57.9	57.9	2.8	0.0	0.135986	115533	116714	1715-1715	0.500000	58.9
ndhH_p3	ndhH	3	GGTTTGCGGAAAATTCTGGG	AAGAATTCCCAAAGGCCCAG	20	20	50.0	50.0	57.9	58.1	2.8	0.0	0.136072	115533	116714	1619-1619	0.500000	58.9
ndhH_p4	ndhH	4	GGTTTGCGGAAAATTCTGGG	GAAGAATTCCCAAAGGCCCA	20	20	50.0	50.0	57.9	58.1	2.8	0.0	0.141657	115533	116714	1620-1620	0.500000	58.9
ndhH_p5	ndhH	5	GGTTTGCGGAAAATTCTGGG	TGAAGAATTCCCAAAGGCCC	20	20	50.0	50.0	57.9	58.1	2.8	0.0	0.141657	115533	116714	1621-1621	0.500000	58.9
ndhA_p1	ndhA	1	GGCAAAAAGAGGGGGATTCA	TCAAAGTGTATTCGGCCTCG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.067392	116716	118879	2847-2847	0.500000	59.5
ndhA_p2	ndhA	2	TGGCAAAAAGAGGGGGATTC	TCAAAGTGTATTCGGCCTCG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.067392	116716	118879	2848-2848	0.500000	59.5
ndhA_p3	ndhA	3	GGCAAAAAGAGGGGGATTCA	GCGTGGGACAAGGTTATCAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.101196	116716	118879	2766-2766	0.500000	59.2
ndhA_p4	ndhA	4	TGGCAAAAAGAGGGGGATTC	GCGTGGGACAAGGTTATCAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.101196	116716	118879	2767-2767	0.500000	59.2
ndhA_p5	ndhA	5	AGTCGAAGCACCAAAAGGAG	TCAAAGTGTATTCGGCCTCG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.120347	116716	118879	2612-2612	0.500000	59.0
ndhI_p1	ndhI	1	GGAGCCGTATGAGGTGAAAG	AATCATGTATTGGTCCGGGC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.077419	118961	119458	1575-1575	0.500000	59.4
ndhI_p2	ndhI	2	GGAGCCGTATGAGGTGAAAG	ATAAGACCCGGTCCCAGAAA	20	20	55.0	50.0	58.1	58.0	0.0	14.3	0.097542	118961	119458	1606-1606	0.500000	59.2
ndhI_p3	ndhI	3	GGAGCCGTATGAGGTGAAAG	CGCAGCAGCTACAAAATACG	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.135830	118961	119458	1730-1730	0.500000	58.9
ndhI_p4	ndhI	4	GGAGCCGTATGAGGTGAAAG	ATGATCCCATACCAGGACGT	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.161828	118961	119458	1918-1918	0.500000	58.7
ndhI_p5	ndhI	5	GGTTCTGTAATAGCGACGGG	AATCATGTATTGGTCCGGGC	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.169647	118961	119458	1545-1545	0.500000	58.6
ndhI-ndhG_p1	ndhI-ndhG	1	CGAGGCCGAATACACTTTGA	AATCATGTATTGGTCCGGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023326	119459	119810	728-728	0.500000	59.8
ndhI-ndhG_p2	ndhI-ndhG	2	CGAGGCCGAATACACTTTGA	ATAAGACCCGGTCCCAGAAA	20	20	50.0	50.0	58.0	58.0	0.0	14.3	0.043450	119459	119810	759-759	0.500000	59.7
ndhI-ndhG_p3	ndhI-ndhG	3	ATGATAACCTTGTCCCACGC	AATCATGTATTGGTCCGGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.057130	119459	119810	809-809	0.500000	59.5
ndhI-ndhG_p4	ndhI-ndhG	4	ATGATAACCTTGTCCCACGC	ATAAGACCCGGTCCCAGAAA	20	20	50.0	50.0	58.0	58.0	0.0	14.3	0.077254	119459	119810	840-840	0.500000	59.4
ndhI-ndhG_p5	ndhI-ndhG	5	CGAGGCCGAATACACTTTGA	CGCAGCAGCTACAAAATACG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.081738	119459	119810	883-883	0.500000	59.3
ndhE_p1	ndhE	1	TTTCTGGGACCGGGTCTTAT	TACATCCGTCCCAGGGTATC	20	20	50.0	55.0	58.0	58.0	25.4	5.0	0.044237	120578	120883	1393-1393	0.500000	59.6
ndhE_p2	ndhE	2	TTTCTGGGACCGGGTCTTAT	CCGAATTGGTTTAGGAGCGA	20	20	50.0	50.0	58.0	58.0	25.4	0.0	0.057409	120578	120883	1177-1177	0.500000	59.5
ndhE_p3	ndhE	3	TTTCTGGGACCGGGTCTTAT	TCCGAATTGGTTTAGGAGCG	20	20	50.0	50.0	58.0	58.0	25.4	0.0	0.057409	120578	120883	1178-1178	0.500000	59.5
ndhE_p4	ndhE	4	CGTATTTTGTAGCTGCTGCG	TACATCCGTCCCAGGGTATC	20	20	50.0	55.0	57.9	58.0	13.4	5.0	0.082525	120578	120883	1269-1269	0.500000	59.3
ndhE_p5	ndhE	5	CGTATTTTGTAGCTGCTGCG	CCGAATTGGTTTAGGAGCGA	20	20	50.0	50.0	57.9	58.0	13.4	0.0	0.095697	120578	120883	1053-1053	0.500000	59.2
psaC-ndhD_p1	psaC-ndhD	1	GATACCCTGGGACGGATGTA	CCTATTTGCCCGCTGTACAT	20	20	55.0	50.0	58.0	58.0	22.2	0.0	0.038052	121386	121479	664-664	0.500000	59.7
psaC-ndhD_p2	psaC-ndhD	2	CGCTCCTAAACCAATTCGGA	CCTATTTGCCCGCTGTACAT	20	20	50.0	50.0	58.0	58.0	1.5	0.0	0.051223	121386	121479	879-879	0.500000	59.6
psaC-ndhD_p3	psaC-ndhD	3	TCGCTCCTAAACCAATTCGG	CCTATTTGCCCGCTGTACAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051223	121386	121479	880-880	0.500000	59.6
psaC-ndhD_p4	psaC-ndhD	4	GATACCCTGGGACGGATGTA	ACCAATACCCAGAACTCCCA	20	20	55.0	50.0	58.0	57.9	22.2	0.0	0.059117	121386	121479	842-842	0.500000	59.5
psaC-ndhD_p5	psaC-ndhD	5	CGCTCCTAAACCAATTCGGA	ACCAATACCCAGAACTCCCA	20	20	50.0	50.0	58.0	57.9	1.5	0.0	0.072289	121386	121479	1057-1057	0.500000	59.4
ndhD_p1	ndhD	1	GATACCCTGGGACGGATGTA	GAGCTGGGACCCAAAAGAAA	20	20	55.0	50.0	58.0	58.0	22.2	15.9	0.009994	121480	123009	2226-2226	0.500000	59.9
ndhD_p2	ndhD	2	GATACCCTGGGACGGATGTA	AACTAGTGAGAACCCGGACA	20	20	55.0	50.0	58.0	58.0	22.2	0.7	0.010740	121480	123009	1963-1963	0.500000	59.9
ndhD_p3	ndhD	3	GATACCCTGGGACGGATGTA	ACTAGTGAGAACCCGGACAA	20	20	55.0	50.0	58.0	58.0	22.2	0.7	0.010740	121480	123009	1962-1962	0.500000	59.9
ndhD_p4	ndhD	4	CGCTCCTAAACCAATTCGGA	GAGCTGGGACCCAAAAGAAA	20	20	50.0	50.0	58.0	58.0	1.5	15.9	0.023166	121480	123009	2441-2441	0.500000	59.8
ndhD_p5	ndhD	5	TCGCTCCTAAACCAATTCGG	GAGCTGGGACCCAAAAGAAA	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.023166	121480	123009	2442-2442	0.500000	59.8
ccsA_p1	ccsA	1	TGTCCGGGTTCTCACTAGTT	GCGGCATGTCATCTCCTAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051811	123241	124209	1159-1159	0.500000	59.6
ccsA_p2	ccsA	2	TTGTCCGGGTTCTCACTAGT	GCGGCATGTCATCTCCTAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051811	123241	124209	1160-1160	0.500000	59.6
ccsA_p3	ccsA	3	GTAGCTTTTCGATGGCCTCA	GCGGCATGTCATCTCCTAAA	20	20	50.0	50.0	58.0	58.0	3.3	0.0	0.070617	123241	124209	1683-1683	0.500000	59.4
ccsA_p4	ccsA	4	TAGCTTTTCGATGGCCTCAC	GCGGCATGTCATCTCCTAAA	20	20	50.0	50.0	58.0	58.0	3.3	0.0	0.070617	123241	124209	1682-1682	0.500000	59.4
ccsA_p5	ccsA	5	TGTCCGGGTTCTCACTAGTT	CTCGCCATCAATCGACTCAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.078809	123241	124209	1696-1696	0.500000	59.4
ccsA-trnL-UAG_p1	ccsA-trnL-UAG	1	TGATCCACATAAAAGGGCCG	CTCGCCATCAATCGACTCAA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.124773	124210	124318	1114-1114	0.500000	59.0
ccsA-trnL-UAG_p2	ccsA-trnL-UAG	2	TGATCCACATAAAAGGGCCG	GAGTTCAAGACAAGCCGCTA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.131620	124210	124318	664-664	0.500000	58.9
ccsA-trnL-UAG_p3	ccsA-trnL-UAG	3	AACCACTCGGATTGAAGAGC	CTCGCCATCAATCGACTCAA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.174043	124210	124318	1059-1059	0.500000	58.6
ccsA-trnL-UAG_p4	ccsA-trnL-UAG	4	TGATCCACATAAAAGGGCCG	TAGACACGCTGCTCTTAGGA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.175612	124210	124318	632-632	0.500000	58.6
ccsA-trnL-UAG_p5	ccsA-trnL-UAG	5	TGATCCACATAAAAGGGCCG	ACAAGCCGCTATGGTGAAAT	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.180105	124210	124318	655-655	0.500000	58.6
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	TTTAGGAGATGACATGCCGC	AAAAAGCGCACTTCTGCATC	20	20	50.0	45.0	58.0	57.9	0.0	11.5	0.102535	124399	125197	1073-1073	0.500000	59.2
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	TGATCCACATAAAAGGGCCG	AAAAAGCGCACTTCTGCATC	20	20	50.0	45.0	57.9	57.9	0.0	11.5	0.107429	124399	125197	1630-1630	0.500000	59.1
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AACCACTCGGATTGAAGAGC	AAAAAGCGCACTTCTGCATC	20	20	50.0	45.0	57.9	57.9	0.0	11.5	0.156699	124399	125197	1575-1575	0.500000	58.7
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	TCCTAAGAGCAGCGTGTCTA	AAAAAGCGCACTTCTGCATC	20	20	50.0	45.0	57.9	57.9	0.0	11.5	0.180371	124399	125197	1018-1018	0.500000	58.6
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	AAAAGGGCCGCATAGCTTAA	AAAAAGCGCACTTCTGCATC	20	20	45.0	45.0	57.9	57.9	0.0	11.5	0.193904	124399	125197	1620-1620	0.500000	58.4
rpl32-ndhF_p1	rpl32-ndhF	1	TTGAGTCGATTGATGGCGAG	CAAGAGGTCGTGTGAACCAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.125334	125390	126187	1857-1857	0.500000	59.0
rpl32-ndhF_p2	rpl32-ndhF	2	CCCACCTTGGAACTTGGAAA	CAAGAGGTCGTGTGAACCAA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.132550	125390	126187	1634-1634	0.500000	58.9
rpl32-ndhF_p3	rpl32-ndhF	3	AAAACTCCCCACCTTGGAAC	CAAGAGGTCGTGTGAACCAA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.134036	125390	126187	1641-1641	0.500000	58.9
rpl32-ndhF_p4	rpl32-ndhF	4	TTGAGTCGATTGATGGCGAG	GCCCACATACGACGAAGATT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.137506	125390	126187	1468-1468	0.500000	58.9
rpl32-ndhF_p5	rpl32-ndhF	5	CCCACCTTGGAACTTGGAAA	GCCCACATACGACGAAGATT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.144721	125390	126187	1245-1245	0.500000	58.8
ndhF_p1	ndhF	1	CACCCCTTGCAAAAGAGGTA	AGGGTGGAAAGTGAGGAAGA	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.404768	126188	128455	2673-2673	0.500000	56.8
ndhF_p2	ndhF	2	TTCACCCCTTGCAAAAGAGG	AGGGTGGAAAGTGAGGAAGA	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.414993	126188	128455	2675-2675	0.500000	56.7
ndhF_p3	ndhF	3	CACCCCTTGCAAAAGAGGTA	ACAGCTTCCGAAATGAAGGG	20	20	50.0	50.0	57.7	58.2	0.0	3.3	0.459980	126188	128455	2634-2634	0.500000	56.3
ndhF_p4	ndhF	4	TTCACCCCTTGCAAAAGAGG	ACAGCTTCCGAAATGAAGGG	20	20	50.0	50.0	58.3	58.2	0.0	3.3	0.470204	126188	128455	2636-2636	0.500000	56.2
ndhF_p5	ndhF	5	CACCCCTTGCAAAAGAGGTA	TAGGCAGAATACCCTCACCC	20	20	50.0	55.0	57.7	58.2	0.0	0.0	0.483811	126188	128455	2735-2735	0.500000	56.1