primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	TTTCCTTGAAAGGGGTGCTC	20	20	50.0	50.0	58.2	58.0	20.3	9.2	0.173044	4207	5111	1026-1042	1.000000	82.6
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	TTTCCTTGAAAGGGGTGCTC	20	20	50.0	50.0	58.2	58.0	20.3	9.2	0.173044	4207	5111	1025-1041	1.000000	82.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCAGTCGTGGTCTTCCAAAC	TTTCCTTGAAAGGGGTGCTC	20	20	50.0	50.0	57.8	58.0	0.0	9.2	0.238923	4207	5111	1111-1127	1.000000	81.8
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CAGTCGTGGTCTTCCAAACT	TTTCCTTGAAAGGGGTGCTC	20	20	50.0	50.0	57.8	58.0	0.0	9.2	0.245117	4207	5111	1110-1126	1.000000	81.8
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCTAGCCGCACTTAAAAGCC	TTTCCTTGAAAGGGGTGCTC	20	20	50.0	50.0	58.3	58.0	0.0	9.2	0.270461	4207	5111	1040-1056	1.000000	81.8
trnG-GCC_p1	trnG-GCC	1	GCGGCCAACAACCTATCTAA	ATTGGAACTGGCGCAATTTG	20	20	50.0	45.0	58.0	57.9	2.2	10.8	0.115847	9425	10189	2126-2149	1.000000	80.0
trnG-GCC_p2	trnG-GCC	2	GCGGCCAACAACCTATCTAA	GGCGCAATTTGCAGAACTAG	20	20	50.0	50.0	58.0	58.1	2.2	20.6	0.132443	9425	10189	2117-2140	1.000000	79.9
trnG-GCC_p3	trnG-GCC	3	GCGGCCAACAACCTATCTAA	AATCAGCTCCTCTCACGGTA	20	20	50.0	50.0	58.0	57.9	2.2	0.0	0.178077	9425	10189	2004-2027	1.000000	79.6
trnG-GCC_p4	trnG-GCC	4	TCGAGAGAGAGATCCCCAAC	ATTGGAACTGGCGCAATTTG	20	20	55.0	45.0	57.9	57.9	0.0	10.8	0.143217	9425	10189	2187-2187	0.500000	58.9
trnG-GCC_p5	trnG-GCC	5	TCGAGAGAGAGATCCCCAAC	GGCGCAATTTGCAGAACTAG	20	20	55.0	50.0	57.9	58.1	0.0	20.6	0.159813	9425	10189	2178-2178	0.500000	58.7
atpH-atpI_p1	atpH-atpI	1	TCCGATAGAAGCAAGCCCTA	TCCCTGTCATGTTCCTTGGA	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.341112	13974	15008	1208-1209	1.000000	77.6
atpH-atpI_p2	atpH-atpI	2	AACGGAAGCAGCAGAAATCA	TCCCTGTCATGTTCCTTGGA	20	20	45.0	50.0	58.1	58.3	0.0	0.0	0.362752	13974	15008	1169-1170	1.000000	77.4
atpH-atpI_p3	atpH-atpI	3	CCGATAGAAGCAAGCCCTAC	TCCCTGTCATGTTCCTTGGA	20	20	55.0	50.0	57.8	58.3	0.0	0.0	0.415315	13974	15008	1207-1208	1.000000	77.0
atpH-atpI_p4	atpH-atpI	4	GATAGAAGCAAGCCCTACGG	TCCCTGTCATGTTCCTTGGA	20	20	55.0	50.0	57.8	58.3	0.0	0.0	0.415315	13974	15008	1205-1206	1.000000	77.0
atpH-atpI_p5	atpH-atpI	5	GCCAATCCAGCAGCAATAAC	TCCCTGTCATGTTCCTTGGA	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.473359	13974	15008	1186-1187	1.000000	76.6
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	ATTGCCTCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.174726	27302	28465	1252-1252	1.000000	79.1
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	ATTGCCTCATTTCCATCCCC	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	57.9	58.4	0.0	14.6	0.448089	27302	28465	1234-1234	1.000000	76.9
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	ATTGCCTCATTTCCATCCCC	TTTGTTGATCAGGCGACACC	20	20	50.0	50.0	57.9	58.8	0.0	0.0	0.857777	27302	28465	1271-1271	1.000000	73.6
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	ATTGCCTCATTTCCATCCCC	GACACCCGGATTTGAACTGG	20	20	50.0	55.0	57.9	58.8	0.0	0.7	0.927329	27302	28465	1257-1257	1.000000	73.1
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CTCATTTCCATCCCCGAGC	CCGGATTTGAACTGGGGAAA	19	20	57.9	50.0	58.0	58.1	0.0	0.0	1.094688	27302	28465	1247-1247	1.000000	71.8
petN-psbM_p1	petN-psbM	1	ACTCGTAGTGTGGGGAAGAA	AGTTCCTACCGCTTTTCTACT	20	21	50.0	42.9	58.0	56.4	0.0	0.0	2.616493	29449	30295	936-950	1.000000	59.4
petN-psbM_p2	petN-psbM	2	TCGTAGTGTGGGGAAGAAGT	AGTTCCTACCGCTTTTCTACT	20	21	50.0	42.9	58.0	56.4	0.0	0.0	2.616493	29449	30295	934-948	1.000000	59.4
petN-psbM_p3	petN-psbM	3	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTTCCTACCGCT	20	20	50.0	45.0	58.0	55.4	0.0	0.0	2.635092	29449	30295	943-957	1.000000	59.3
petN-psbM_p4	petN-psbM	4	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACCGCT	20	20	50.0	45.0	58.0	55.4	0.0	0.0	2.635092	29449	30295	941-955	1.000000	59.3
petN-psbM_p5	petN-psbM	5	CGTAGTGTGGGGAAGAAGTG	AGTTCCTACCGCTTTTCTACT	20	21	55.0	42.9	57.9	56.4	0.0	0.0	2.691409	29449	30295	933-947	1.000000	58.8
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	20	20	50.0	50.0	58.5	57.8	7.1	0.0	0.716274	31004	31111	178-178	1.000000	75.6
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	20	20	50.0	50.0	58.5	57.8	7.1	0.0	0.717825	31004	31111	177-177	1.000000	75.6
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.5	58.4	7.1	0.0	0.828828	31004	31111	199-199	1.000000	74.6
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	20	20	50.0	50.0	58.5	57.6	7.1	0.0	0.852505	31004	31111	198-198	1.000000	74.4
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	CGGTGCTCTGACCAATTGAA	GATTCTTCCTGGGTCGATGC	20	20	50.0	55.0	58.5	58.4	7.1	0.0	0.889785	31004	31111	232-232	1.000000	73.9
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	20	20	55.0	50.0	58.1	58.1	7.6	0.0	0.177181	32172	33524	1513-1521	1.000000	79.3
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	ACATAGTGACCCGTTCAGGA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.180092	32172	33524	1561-1569	1.000000	79.3
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.199336	32172	33524	1516-1524	1.000000	79.1
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	32172	33524	1503-1511	1.000000	78.3
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	ACATAGTGACCCGTTCAGGA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.313048	32172	33524	1551-1559	1.000000	78.2
psaA-ycf3_p1	psaA-ycf3	1	TGAGAAATGACCCGGTTTGG	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.136672	42411	43157	944-944	1.000000	79.2
psaA-ycf3_p2	psaA-ycf3	2	TGAGAAATGACCCGGTTTGG	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	58.1	58.0	0.0	18.7	0.140786	42411	43157	926-926	1.000000	79.2
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	42411	43157	925-925	1.000000	79.1
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	57.9	58.0	4.1	18.7	0.160416	42411	43157	907-907	1.000000	79.1
psaA-ycf3_p5	psaA-ycf3	5	TGAGAAATGACCCGGTTTGG	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.259531	42411	43157	873-873	1.000000	78.3
ndhC-trnM-CAU_p1	ndhC-trnM-CAU	1	GGGCTAAGATCCCCGAAATG	CCCGCCGTATGAAAGCAATA	20	20	55.0	50.0	58.1	58.4	0.0	0.0	0.513766	51002	52719	1850-1859	1.000000	77.0
ndhC-trnM-CAU_p2	ndhC-trnM-CAU	2	GGGCTAAGATCCCCGAAATG	CCGCCGTATGAAAGCAATAC	20	20	55.0	50.0	58.1	57.3	0.0	0.0	0.765719	51002	52719	1849-1858	1.000000	75.0
ndhC-trnM-CAU_p3	ndhC-trnM-CAU	3	TTCTCCGGCCCCTTTCTAAT	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	58.4	58.4	0.0	0.0	0.819557	51002	52719	1872-1881	1.000000	74.5
ndhC-trnM-CAU_p4	ndhC-trnM-CAU	4	GGGCTAAGATCCCCGAAATG	TTTGAACCAATGACTCCCGC	20	20	55.0	50.0	58.1	58.8	0.0	0.0	0.858538	51002	52719	1865-1874	1.000000	74.2
ndhC-trnM-CAU_p5	ndhC-trnM-CAU	5	TTTCTCCGGCCCCTTTCTAA	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	58.6	58.4	0.0	0.0	1.047057	51002	52719	1873-1882	1.000000	72.7
atpB-rbcL_p1	atpB-rbcL	1	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	58.1	58.3	0.0	0.0	0.390437	54898	55676	869-869	1.000000	77.2
atpB-rbcL_p2	atpB-rbcL	2	ATGATTTGGACGATACGCCC	AACACCCGCTTTGAATCCAA	20	20	50.0	45.0	58.1	58.3	0.0	0.0	0.422541	54898	55676	910-910	1.000000	76.9
atpB-rbcL_p3	atpB-rbcL	3	CCGGACCTATGATTTGGACG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	58.1	58.3	0.0	0.0	0.435629	54898	55676	918-918	1.000000	76.8
atpB-rbcL_p4	atpB-rbcL	4	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	20	20	55.0	45.0	57.8	58.3	0.0	0.0	0.457837	54898	55676	940-940	1.000000	76.6
atpB-rbcL_p5	atpB-rbcL	5	GGAAACCCCAGAACCAGAAG	TAACACCCGCTTTGAATCCA	20	20	55.0	45.0	58.1	57.4	0.0	0.0	0.659357	54898	55676	870-870	1.000000	75.0
psbE-petL_p1	psbE-petL	1	AAAGGATCAAAACGGCCAGT	TGATCACCACGCCTGTATTG	20	20	45.0	50.0	58.0	58.0	2.2	0.0	0.027388	65601	66526	1516-1524	1.000000	81.3
psbE-petL_p2	psbE-petL	2	AAGGATCAAAACGGCCAGTT	TGATCACCACGCCTGTATTG	20	20	45.0	50.0	58.0	58.0	2.2	0.0	0.027388	65601	66526	1515-1523	1.000000	81.3
psbE-petL_p3	psbE-petL	3	AAAGGATCAAAACGGCCAGT	AGCGCTCTTAGTTCAGTTCG	20	20	45.0	50.0	58.0	58.0	2.2	2.6	0.020651	65601	66526	1726-1734	1.000000	81.2
psbE-petL_p4	psbE-petL	4	AAGGATCAAAACGGCCAGTT	AGCGCTCTTAGTTCAGTTCG	20	20	45.0	50.0	58.0	58.0	2.2	2.6	0.020651	65601	66526	1725-1733	1.000000	81.2
psbE-petL_p5	psbE-petL	5	AAGGATCAAAACGGCCAGTT	GATCACCACGCCTGTATTGT	20	20	45.0	50.0	58.0	58.0	2.2	0.0	0.028799	65601	66526	1514-1522	1.000000	81.2
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	ACCCGTGACATTTTGTACCC	GCTAAAGCCCCGAACCATAG	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.505424	67346	67753	524-531	1.000000	77.9
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CCCGTGACATTTTGTACCCA	GCTAAAGCCCCGAACCATAG	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.506768	67346	67753	523-530	1.000000	77.9
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ACAGGATTTGAACCCGTGAC	GCTAAAGCCCCGAACCATAG	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.513460	67346	67753	535-542	1.000000	77.8
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAGGATTTGAACCCGTGACA	GCTAAAGCCCCGAACCATAG	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.514793	67346	67753	534-541	1.000000	77.8
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TGACAGGATTTGAACCCGTG	GCTAAAGCCCCGAACCATAG	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.514793	67346	67753	537-544	1.000000	77.8
psbT-psbN_p1	psbT-psbN	1	GCGAACCACCTAAAGTTCCA	TCGAGGAACATGGGGACTAG	20	20	50.0	55.0	58.1	58.2	0.0	0.0	0.336451	74957	75021	117-117	1.000000	78.7
psbT-psbN_p2	psbT-psbN	2	GCGAACCACCTAAAGTTCCA	TTCGAGGAACATGGGGACTA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.354394	74957	75021	118-118	1.000000	78.5
psbT-psbN_p3	psbT-psbN	3	TCGCGAACCACCTAAAGTTC	TCGAGGAACATGGGGACTAG	20	20	50.0	55.0	58.2	58.2	6.2	0.0	0.436326	74957	75021	119-119	1.000000	77.9
psbT-psbN_p4	psbT-psbN	4	GCGAACCACCTAAAGTTCCA	GGGTACGCCTTATATACCGC	20	20	50.0	55.0	58.1	57.7	0.0	0.0	0.397376	74957	75021	172-172	1.000000	77.8
psbT-psbN_p5	psbT-psbN	5	GCGAACCACCTAAAGTTCCA	CATTCGAGGAACATGGGGAC	20	20	50.0	55.0	58.1	58.3	0.0	0.0	0.444694	74957	75021	120-120	1.000000	77.8
psbH-petB_p1	psbH-petB	1	TCCTTTGATGGGTGTTGCAA	CGAGACGTTCTTCGAACCAA	20	20	45.0	50.0	57.9	58.2	0.0	11.2	0.316750	75481	76302	974-974	1.000000	78.0
psbH-petB_p2	psbH-petB	2	TCCTTTGATGGGTGTTGCAA	ATATCATCCGCAATCGCCTG	20	20	45.0	50.0	57.9	58.3	0.0	0.0	0.348669	75481	76302	999-999	1.000000	77.7
psbH-petB_p3	psbH-petB	3	AACGACTCCTTTGATGGGTG	CGAGACGTTCTTCGAACCAA	20	20	50.0	50.0	57.8	58.2	0.0	11.2	0.422585	75481	76302	980-980	1.000000	77.1
psbH-petB_p4	psbH-petB	4	CGACTCCTTTGATGGGTGTT	CGAGACGTTCTTCGAACCAA	20	20	50.0	50.0	57.8	58.2	0.0	11.2	0.422585	75481	76302	978-978	1.000000	77.1
psbH-petB_p5	psbH-petB	5	CGACTCCTTTGATGGGTGTT	ATATCATCCGCAATCGCCTG	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.454503	75481	76302	1003-1003	1.000000	76.8
petB-petD_p1	petB-petD	1	TTGCCACTTCTTACTGCTGT	GATCCATCCCCCGAAAGAAC	20	20	45.0	55.0	57.4	58.0	0.7	0.0	0.669776	76963	77844	981-987	1.000000	75.5
petB-petD_p2	petB-petD	2	TTGCCACTTCTTACTGCTGT	AGATCCATCCCCCGAAAGAA	20	20	45.0	50.0	57.4	58.1	0.7	0.0	0.745165	76963	77844	982-988	1.000000	74.9
petB-petD_p3	petB-petD	3	ATTGCCACTTCTTACTGCTGT	GATCCATCCCCCGAAAGAAC	21	20	42.9	55.0	57.9	58.0	0.7	0.0	1.177400	76963	77844	982-988	1.000000	71.5
petB-petD_p4	petB-petD	4	ATTGCCACTTCTTACTGCTGT	AGATCCATCCCCCGAAAGAA	21	20	42.9	50.0	57.9	58.1	0.7	0.0	1.252789	76963	77844	983-989	1.000000	70.9
petB-petD_p5	petB-petD	5	TTGCCACTTCTTACTGCTGT	TAGATCCATCCCCCGAAAGA	20	20	45.0	50.0	57.4	57.2	0.7	0.0	1.425819	76963	77844	983-989	1.000000	69.5
ndhF-rpl32_p1	ndhF-rpl32	1	ACAAAAGGTATGATCCACGCA	AAACGCTTTTAACGATGCCC	21	20	42.9	45.0	57.9	57.7	0.0	0.0	1.407609	112016	112706	828-848	1.000000	69.7
ndhF-rpl32_p2	ndhF-rpl32	2	ACAAAAGGTATGATCCACGCA	CGCTTTTAACGATGCCCAAC	21	20	42.9	50.0	57.9	58.4	0.0	0.0	1.453022	112016	112706	825-845	1.000000	69.3
ndhF-rpl32_p3	ndhF-rpl32	3	ACAAAAGGTATGATCCACGCA	CCAACATCCCTTCCTCTTCC	21	20	42.9	55.0	57.9	57.6	0.0	0.0	1.493134	112016	112706	810-830	1.000000	69.0
ndhF-rpl32_p4	ndhF-rpl32	4	ACAAAAGGTATGATCCACGCA	CCCAACATCCCTTCCTCTTC	21	20	42.9	55.0	57.9	57.6	0.0	0.0	1.493134	112016	112706	811-831	1.000000	69.0
ndhF-rpl32_p5	ndhF-rpl32	5	AAAGAAGAAGTCCCACTGCT	AAACGCTTTTAACGATGCCC	20	20	45.0	45.0	56.7	57.7	0.0	0.0	1.675728	112016	112706	872-892	1.000000	67.5
ccsA_p1	ccsA	1	GAGTTCAAGACAAGCCGCTA	TCAGTAGCTTTTCGATGGCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.104462	113884	114855	1754-1759	1.000000	79.9
ccsA_p2	ccsA	2	GAGTTCAAGACAAGCCGCTA	CAGTAGCTTTTCGATGGCCT	20	20	50.0	50.0	57.9	58.0	0.0	3.3	0.110124	113884	114855	1753-1758	1.000000	79.9
ccsA_p3	ccsA	3	GAGTTCAAGACAAGCCGCTA	TGGTGCTGCACTCTTTTTCT	20	20	50.0	45.0	57.9	57.9	0.0	29.5	0.137423	113884	114855	1864-1869	1.000000	79.6
ccsA_p4	ccsA	4	TAGACACGCTGCTCTTAGGA	TCAGTAGCTTTTCGATGGCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.148453	113884	114855	1722-1727	1.000000	79.6
ccsA_p5	ccsA	5	ACAAGCCGCTATGGTGAAAT	TCAGTAGCTTTTCGATGGCC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.152946	113884	114855	1745-1750	1.000000	79.6