primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	195	558	433-433	1.000000	78.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	ATACTTATGGGCGAACGACG	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.204552	195	558	495-495	1.000000	78.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	195	558	447-447	1.000000	78.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	195	558	446-446	1.000000	78.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	195	558	448-448	1.000000	78.3
psbA_p1	psbA	1	AAGAAATCGGTAATGCCCCT	AGAATTCGTGTGCTTGGGAG	20	20	45.0	50.0	56.9	58.2	0.0	0.0	1.310026	559	1620	1176-1176	1.000000	69.5
psbA_p2	psbA	2	AAGAAATCGGTAATGCCCCT	AGAGAATTCGTGTGCTTGGG	20	20	45.0	50.0	56.9	58.2	0.0	0.0	1.310026	559	1620	1178-1178	1.000000	69.5
psbA_p3	psbA	3	AGAAATCGGTAATGCCCCTT	AGAATTCGTGTGCTTGGGAG	20	20	45.0	50.0	56.9	58.2	0.0	0.0	1.310026	559	1620	1175-1175	1.000000	69.5
psbA_p4	psbA	4	AGAAATCGGTAATGCCCCTT	AGAGAATTCGTGTGCTTGGG	20	20	45.0	50.0	56.9	58.2	0.0	0.0	1.310026	559	1620	1177-1177	1.000000	69.5
psbA_p5	psbA	5	AGAAATCGGTAATGCCCCTT	GAGAATTCGTGTGCTTGGGA	20	20	45.0	50.0	56.9	58.2	0.0	0.0	1.315129	559	1620	1176-1176	1.000000	69.5
matK_p1	matK	1	TCAACCCTTACTGTATAAGCA	CCTTGTTTTGACTGTATCGCA	21	21	38.1	42.9	54.0	56.8	0.0	0.0	7.185447	2147	3664	1722-1722	1.000000	40.0
matK_p2	matK	2	TCAACCCTTACTGTATAAGCA	ACCTTGTTTTGACTGTATCGC	21	21	38.1	42.9	54.0	56.8	0.0	0.0	7.187481	2147	3664	1723-1723	1.000000	40.0
matK_p3	matK	3	TCAACCCTTACTGTATAAGCA	CCTTGTTTTGACTGTATCGCAC	21	22	38.1	45.5	54.0	58.2	0.0	0.0	7.207586	2147	3664	1722-1722	1.000000	40.0
matK_p4	matK	4	TCAACCCTTACTGTATAAGCA	ACCTTGTTTTGACTGTATCGCA	21	22	38.1	40.9	54.0	58.5	0.0	0.0	7.545308	2147	3664	1723-1723	1.000000	40.0
matK_p5	matK	5	TCAACCCTTACTGTATAAGCAT	CCTTGTTTTGACTGTATCGCA	22	21	36.4	42.9	54.6	56.8	0.0	0.0	7.565392	2147	3664	1722-1722	1.000000	40.0
matK-trnK-UUU_p1	matK-trnK-UUU	1	TCTTGCCAAGACCTATCTAGT	TGGGGTTGCTAACTCAATGG	21	20	42.9	50.0	55.5	57.8	2.2	0.0	3.716150	3665	4380	802-802	1.000000	50.3
matK-trnK-UUU_p2	matK-trnK-UUU	2	TGCCAAGACCTATCTAGTTCT	TGGGGTTGCTAACTCAATGG	21	20	42.9	50.0	55.5	57.8	0.0	0.0	3.716150	3665	4380	799-799	1.000000	50.3
matK-trnK-UUU_p3	matK-trnK-UUU	3	TCTTGCCAAGACCTATCTAGT	GGGGTTGCTAACTCAATGGT	21	20	42.9	50.0	55.5	57.8	2.2	2.1	3.717716	3665	4380	801-801	1.000000	50.3
matK-trnK-UUU_p4	matK-trnK-UUU	4	TGCCAAGACCTATCTAGTTCT	GGGGTTGCTAACTCAATGGT	21	20	42.9	50.0	55.5	57.8	0.0	2.1	3.717716	3665	4380	798-798	1.000000	50.3
matK-trnK-UUU_p5	matK-trnK-UUU	5	TCTTGCCAAGACCTATCTAGTTC	TGGGGTTGCTAACTCAATGG	23	20	43.5	50.0	57.4	57.8	2.2	0.0	3.814375	3665	4380	802-802	1.000000	49.5
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TCTAGCCGCACTTAAAAGCC	AAGAGGGCAGAGGTTTTTCA	20	20	50.0	45.0	58.3	56.9	0.0	0.0	1.316701	4418	5164	834-849	1.000000	70.1
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TCTAGCCGCACTTAAAAGCC	AGAGGGCAGAGGTTTTTCAA	20	20	50.0	45.0	58.3	56.9	0.0	0.0	1.316701	4418	5164	833-848	1.000000	70.1
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCTAGCCGCACTTAAAAGCC	AGGGCAGAGGTTTTTCAAGA	20	20	50.0	45.0	58.3	56.9	0.0	0.0	1.316701	4418	5164	831-846	1.000000	70.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGCACTTAAAAGCCGAGTA	AGAGGGCAGAGGTTTTTCAA	20	20	50.0	45.0	58.3	56.9	5.3	0.0	1.333670	4418	5164	828-843	1.000000	70.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCGCACTTAAAAGCCGAGTA	AGGGCAGAGGTTTTTCAAGA	20	20	50.0	45.0	58.3	56.9	5.3	0.0	1.333670	4418	5164	826-841	1.000000	70.0
rps16_p1	rps16	1	TGTGTTGGATTGGCACTACA	CCGGTTCCAAATTCGAGGAA	20	20	45.0	50.0	57.4	58.2	0.0	0.0	0.831609	5165	6301	1260-1260	1.000000	73.3
rps16_p2	rps16	2	TGTGTTGGATTGGCACTACA	TGACTGTCCTGACTGAACCA	20	20	45.0	50.0	57.4	58.2	0.0	5.2	0.859454	5165	6301	1318-1318	1.000000	73.1
rps16_p3	rps16	3	TGTGTTGGATTGGCACTACA	TACCGGTTCCAAATTCGAGG	20	20	45.0	50.0	57.4	57.6	0.0	15.0	1.037645	5165	6301	1262-1262	1.000000	71.7
rps16_p4	rps16	4	TGTGTTGGATTGGCACTACA	CTGACTGTCCTGACTGAACC	20	20	45.0	55.0	57.4	57.6	0.0	5.2	1.094928	5165	6301	1319-1319	1.000000	71.2
rps16_p5	rps16	5	TGTGTTGGATTGGCACTACA	GCATACCGGTTCCAAATTCG	20	20	45.0	50.0	57.4	57.5	0.0	15.0	1.116215	5165	6301	1265-1265	1.000000	71.1
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TTCCTCGAATTTGGAACCGG	TCTGTTTCTAAAGTTTAGTATTGG	20	24	50.0	29.2	58.2	52.8	0.0	19.6	9.425752	6502	6901	620-620	1.000000	40.0
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TGGTTCAGTCAGGACAGTCA	TCTGTTTCTAAAGTTTAGTATTGG	20	24	50.0	29.2	58.2	52.8	2.2	19.6	9.453598	6502	6901	562-562	1.000000	40.0
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CCTCGAATTTGGAACCGGTA	TCTGTTTCTAAAGTTTAGTATTGG	20	24	50.0	29.2	57.6	52.8	8.0	19.6	9.631789	6502	6901	618-618	1.000000	40.0
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TTCCTCGAATTTGGAACCGG	TTCTGTTTCTAAAGTTTAGTATTGG	20	25	50.0	28.0	58.2	53.5	0.0	19.6	9.644950	6502	6901	621-621	1.000000	40.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TGGTTCAGTCAGGACAGTCA	TTCTGTTTCTAAAGTTTAGTATTGG	20	25	50.0	28.0	58.2	53.5	2.2	19.6	9.672795	6502	6901	563-563	1.000000	40.0
trnQ-UUG-psbK_p1	trnQ-UUG-psbK	1	CATATGCTCTGGGACGGAAG	AGGGCAGAATTAAGACAGATCA	20	22	55.0	40.9	57.8	56.9	0.0	0.0	3.281344	7037	7286	447-447	1.000000	53.9
trnQ-UUG-psbK_p2	trnQ-UUG-psbK	2	GACCAAAACCCGATGCCTTA	AGGGCAGAATTAAGACAGATCA	20	22	50.0	40.9	58.2	56.9	0.0	0.0	3.291542	7037	7286	405-405	1.000000	53.9
trnQ-UUG-psbK_p3	trnQ-UUG-psbK	3	TACATATGCTCTGGGACGGA	AGGGCAGAATTAAGACAGATCA	20	22	50.0	40.9	57.6	56.9	1.6	0.0	3.495535	7037	7286	449-449	1.000000	52.2
trnQ-UUG-psbK_p4	trnQ-UUG-psbK	4	AAAACCCGATGCCTTACCAC	AGGGCAGAATTAAGACAGATCA	20	22	50.0	40.9	58.5	56.9	0.0	0.0	3.579143	7037	7286	401-401	1.000000	51.6
trnQ-UUG-psbK_p5	trnQ-UUG-psbK	5	ACCAAAACCCGATGCCTTAC	AGGGCAGAATTAAGACAGATCA	20	22	50.0	40.9	58.5	56.9	0.0	0.0	3.579143	7037	7286	404-404	1.000000	51.6
trnG-UCC_p1	trnG-UCC	1	CTCGTGATTTGTATCCAAGGGT	ACTTTGGTCTCATTCGGCTC	22	20	45.5	50.0	58.1	57.9	0.0	0.0	2.226695	9164	9563	516-1255	1.000000	62.2
trnG-UCC_p2	trnG-UCC	2	CTCGTGATTTGTATCCAAGGGT	AACTTTGGTCTCATTCGGCT	22	20	45.5	45.0	58.1	57.4	0.0	0.0	2.694713	9164	9563	517-1256	1.000000	58.4
trnG-UCC_p3	trnG-UCC	3	CTCGTGATTTGTATCCAAGGGT	CTTTGGTCTCATTCGGCTCC	22	20	45.5	55.0	58.1	58.6	0.0	0.0	2.752520	9164	9563	515-1254	1.000000	58.0
trnG-UCC_p4	trnG-UCC	4	CTCGTGATTTGTATCCAAGGGT	TGTTAGCTTTTTGGATGAATGCA	22	23	45.5	34.8	58.1	57.5	0.0	0.0	5.591622	9214	9463	413-1152	1.000000	40.0
trnG-UCC_p5	trnG-UCC	5	CTCGTGATTTGTATCCAAGGGT	TCTAAGGTGGGAACGAAATT	22	20	45.5	40.0	58.1	54.3	0.0	0.0	5.864225	9214	9463	462-1201	1.000000	40.0
atpA_p1	atpA	1	GTTCAAATCCTATTGGACGCA	GTTTGGTGCGATGAACGAAA	21	20	42.9	45.0	56.6	57.6	0.0	0.0	2.788667	10063	11586	1742-1742	1.000000	57.7
atpA_p2	atpA	2	GTTCAAATCCTATTGGACGCA	CATGTTTGGTGCGATGAACG	21	20	42.9	50.0	56.6	58.4	0.0	0.0	2.790234	10063	11586	1745-1745	1.000000	57.7
atpA_p3	atpA	3	GTTCAAATCCTATTGGACGCA	TATTGGCATGTTTGGTGCGA	21	20	42.9	45.0	56.6	58.5	0.0	0.0	2.856578	10063	11586	1751-1751	1.000000	57.1
atpA_p4	atpA	4	GTTCAAATCCTATTGGACGCA	ATGTTTGGTGCGATGAACGA	21	20	42.9	45.0	56.6	58.5	0.0	0.0	2.877410	10063	11586	1744-1744	1.000000	57.0
atpA_p5	atpA	5	GTTCAAATCCTATTGGACGCA	ATATTGGCATGTTTGGTGCG	21	20	42.9	45.0	56.6	57.4	0.0	0.0	2.978462	10063	11586	1752-1752	1.000000	56.2
atpF_p1	atpF	1	TTTCGTCTGCTCGAATGGTT	AGAAGTAGCAAAGAAGTGAA	20	20	45.0	35.0	57.8	52.0	11.2	0.0	6.116590	11654	12972	1474-1474	1.000000	40.0
atpF_p2	atpF	2	TTTCGTCTGCTCGAATGGTT	AGAAGTAGCAAAGAAGTGAAA	20	21	45.0	33.3	57.8	53.0	11.2	0.0	6.153564	11654	12972	1474-1474	1.000000	40.0
atpF_p3	atpF	3	TCGTCTGCTCGAATGGTTAC	AGAAGTAGCAAAGAAGTGAA	20	20	50.0	35.0	57.7	52.0	0.0	0.0	6.226142	11654	12972	1472-1472	1.000000	40.0
atpF_p4	atpF	4	TTTCGTCTGCTCGAATGGTT	AGCAAAGAAGTGAAATAATACAACGA	20	26	45.0	30.8	57.8	57.9	11.2	0.0	6.258615	11654	12972	1468-1468	1.000000	40.0
atpF_p5	atpF	5	TCGTCTGCTCGAATGGTTAC	AGAAGTAGCAAAGAAGTGAAA	20	21	50.0	33.3	57.7	53.0	0.0	0.0	6.263115	11654	12972	1472-1472	1.000000	40.0
psaA-ycf3_p1	psaA-ycf3	1	TGAAAAATGACCGGGTCTGG	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	58.1	58.0	26.4	0.0	0.136672	42621	43529	659-1106	1.000000	82.8
psaA-ycf3_p2	psaA-ycf3	2	TGAAAAATGACCGGGTCTGG	ATTGGTTGAAGATCACGGGG	20	20	50.0	50.0	58.1	57.9	26.4	0.0	0.224484	42621	43529	659-1040	1.000000	82.2
psaA-ycf3_p3	psaA-ycf3	3	TGAAAAATGACCGGGTCTGG	CGATCAAGCCGCTGAGTATT	20	20	50.0	50.0	58.1	58.1	26.4	0.0	0.237622	42621	43529	659-1108	1.000000	82.0
psaA-ycf3_p4	psaA-ycf3	4	TGAAAAATGACCGGGTCTGG	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	58.1	58.3	26.4	0.0	0.356647	42621	43529	659-1102	1.000000	81.1
psaA-ycf3_p5	psaA-ycf3	5	TGAAAAATGACCGGGTCTGG	CAAGCCGCTGAGTATTGGAA	20	20	50.0	50.0	58.1	58.3	26.4	0.0	0.357875	42621	43529	657-1104	1.000000	81.0
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	AGCCAATTAAGTCCGTAGCG	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.196808	48017	48800	857-939	1.000000	79.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	AGCCAATTAAGTCCGTAGCG	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.304561	48017	48800	889-971	1.000000	79.0
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	48017	48800	845-927	1.000000	78.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	GCCAATTAAGTCCGTAGCGT	20	20	55.0	50.0	57.9	58.4	0.0	0.0	0.484236	48017	48800	856-938	1.000000	77.6
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	48017	48800	877-959	1.000000	77.6