primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGTCGCACTTAAAAGCCGAG	AAGGCGGGTGTTTTTATGGA	20	20	50.0	45.0	58.6	57.7	6.4	0.0	0.866610	4429	5605	1285-1488	1.000000	85.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AGTCGCACTTAAAAGCCGAG	AGGCGGGTGTTTTTATGGAA	20	20	50.0	45.0	58.6	57.7	6.4	0.0	0.866610	4429	5605	1284-1487	1.000000	85.0
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	GAGTCGCACTTAAAAGCCGA	AAGGCGGGTGTTTTTATGGA	20	20	50.0	45.0	58.6	57.7	3.0	0.0	0.870661	4429	5605	1286-1489	1.000000	85.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	GAGTCGCACTTAAAAGCCGA	AGGCGGGTGTTTTTATGGAA	20	20	50.0	45.0	58.6	57.7	3.0	0.0	0.870661	4429	5605	1285-1488	1.000000	85.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AGTCGCACTTAAAAGCCGAG	GGCGGGTGTTTTTATGGAAC	20	20	50.0	50.0	58.6	57.6	6.4	0.0	0.927864	4429	5605	1283-1486	1.000000	84.6
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	CCTATCTAACGATCCAGGACG	TGGACTAAAGCGTCGGATTG	21	20	52.4	50.0	57.2	58.0	9.2	0.0	1.838128	8764	9202	495-566	1.000000	74.6
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	CCTATCTAACGATCCAGGACG	GTGGACTAAAGCGTCGGATT	21	20	52.4	50.0	57.2	58.0	9.2	0.0	1.839516	8764	9202	496-567	1.000000	74.6
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	CCTATCTAACGATCCAGGACG	AAGGGCCCTTTTCAATTGGT	21	20	52.4	45.0	57.2	57.9	9.2	32.5	1.950414	8764	9202	540-611	1.000000	73.3
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	CCTATCTAACGATCCAGGACG	AGGGCCCTTTTCAATTGGTT	21	20	52.4	45.0	57.2	57.9	9.2	22.3	1.950414	8764	9202	539-610	1.000000	73.3
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	CCTATCTAACGATCCAGGACG	TTTTTCGTACCGAGGGTTCG	21	20	52.4	50.0	57.2	58.2	9.2	7.4	2.051501	8764	9202	458-529	1.000000	73.3
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	TGAATCAAACGAGGGATCCC	20	20	50.0	50.0	58.0	57.3	0.0	24.6	0.714678	9291	10367	1090-1189	1.000000	82.8
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	TGAATCAAACGAGGGATCCC	20	20	50.0	50.0	58.0	57.3	0.0	24.6	0.716066	9291	10367	1089-1188	1.000000	82.8
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACGAGGGATCCC	20	20	50.0	50.0	58.2	57.3	0.0	24.6	0.861444	9291	10367	1144-1243	1.000000	81.4
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CAATCCGACGCTTTAGTCCA	GGATCCATAGAACGAATCGCA	20	21	50.0	47.6	58.0	58.0	0.0	7.0	1.031212	9291	10367	1058-1157	1.000000	80.4
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AATCCGACGCTTTAGTCCAC	GGATCCATAGAACGAATCGCA	20	21	50.0	47.6	58.0	58.0	0.0	7.0	1.032600	9291	10367	1057-1156	1.000000	80.4
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	GGTTCAAATCCTATTGGACGC	CATTCACTCGGGAAGCAGAA	21	20	47.6	50.0	57.3	57.9	0.0	0.0	1.791932	11560	12061	556-662	1.000000	73.9
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GGTTCAAATCCTATTGGACGC	AAGACATTCACTCGGGAAGC	21	20	47.6	50.0	57.3	57.9	0.0	0.0	1.793395	11560	12061	560-666	1.000000	73.8
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	GGTTCAAATCCTATTGGACGC	GGAAGCAGAAGCCCTTTTGA	21	20	47.6	50.0	57.3	58.4	0.0	3.1	2.071968	11560	12061	546-652	1.000000	71.7
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	GGTTCAAATCCTATTGGACGC	ATTCACTCGGGAAGCAGAAG	21	20	47.6	50.0	57.3	57.6	0.0	0.0	2.086062	11560	12061	555-661	1.000000	71.5
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	TGGATAGGACAGAGGTCTTCT	CATTCACTCGGGAAGCAGAA	21	20	47.6	50.0	56.9	57.9	0.0	0.0	2.150746	11560	12061	591-697	1.000000	70.8
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CTTCTCCTGATGGTTGGTCG	GCGTCTTCTCTTTGGCAAAG	20	20	55.0	50.0	58.0	57.7	0.0	29.6	0.338107	40859	41571	789-868	1.000000	85.0
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	CTTCTCCTGATGGTTGGTCG	ATACATAAATAGGGGGCGCG	20	20	55.0	50.0	58.0	57.6	0.0	0.0	0.412325	40859	41571	833-912	1.000000	84.2
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TTGCTTCTCCTGATGGTTGG	GCGTCTTCTCTTTGGCAAAG	20	20	50.0	50.0	57.8	57.7	0.0	29.6	0.527394	40859	41571	792-871	1.000000	83.5
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	TCCTGATGGTTGGTCGAGTA	GCGTCTTCTCTTTGGCAAAG	20	20	50.0	50.0	57.8	57.7	0.0	29.6	0.547753	40859	41571	785-864	1.000000	83.3
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	GCTTCTCCTGATGGTTGGTC	GCGTCTTCTCTTTGGCAAAG	20	20	55.0	50.0	58.3	57.7	0.0	29.6	0.578376	40859	41571	790-869	1.000000	83.1
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	51416	52905	1348-1572	1.000000	86.2
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	51416	52905	1356-1580	1.000000	85.7
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	AAATGCGACGCTCTAACCTC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.4	58.0	22.1	0.0	0.363047	51416	52905	1320-1544	1.000000	85.4
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	AAATGCGACGCTCTAACCTC	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.4	58.1	22.1	0.0	0.427856	51416	52905	1328-1552	1.000000	84.8
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	20	20	50.0	50.0	57.8	58.2	15.5	10.2	0.480302	51416	52905	1362-1586	1.000000	84.3
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGGCCCTTTATTAATCGGGG	AAAAGATGCACGTTGGGTCT	20	20	50.0	45.0	57.3	58.0	15.4	0.0	0.686887	56519	57572	1131-1272	1.000000	82.3
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGGCCCTTTATTAATCGGGG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.3	57.8	15.4	0.0	0.865427	56519	57572	1060-1201	1.000000	81.1
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TGGCCCTTTATTAATCGGGG	GATGCACGTTGGGTCTGTAA	20	20	50.0	50.0	57.3	58.2	15.4	0.0	0.864810	56519	57572	1127-1268	1.000000	80.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGGCCCTTTATTAATCGGGG	ATGCACGTTGGGTCTGTAAA	20	20	50.0	45.0	57.3	57.7	15.4	0.0	0.932149	56519	57572	1126-1267	1.000000	80.4
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGGCCCTTTATTAATCGGGG	ACGTTGGGTCTGTAAAGCAA	20	20	50.0	45.0	57.3	57.7	15.4	0.0	1.003460	56519	57572	1122-1263	1.000000	79.8
accD_p1	accD	1	TCCTTTCGTTTCGTGTTGGA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.7	58.0	0.0	26.9	0.339726	63921	65501	2743-2828	0.666667	72.9
accD_p2	accD	2	TCCTTTCGTTTCGTGTTGGA	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	57.7	57.9	0.0	26.8	0.416260	63921	65501	2749-2834	0.666667	72.3
accD_p3	accD	3	TGACACAACAGGGGGTAAAC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.6	58.0	0.0	26.9	0.378709	63921	65501	2599-2599	0.333333	50.3
accD_p4	accD	4	TGACACAACAGGGGGTAAAC	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.6	57.9	0.0	26.8	0.455243	63921	65501	2605-2605	0.333333	49.7
accD_p5	accD	5	AATTTGACACAACAGGGGGT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.5	58.0	0.0	26.9	0.497060	63921	65501	2603-2603	0.333333	49.4
accD-psaI_p1	accD-psaI	1	GTATGTGCTTCTGGAGGAGC	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.1	58.0	0.0	26.9	0.080258	65502	66076	1034-1053	1.000000	88.9
accD-psaI_p2	accD-psaI	2	GTATGTGCTTCTGGAGGAGC	TTGTTTTTCGAGACCCCGTT	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.094506	65502	66076	1328-1336	1.000000	87.9
accD-psaI_p3	accD-psaI	3	GTATGTGCTTCTGGAGGAGC	ATACGTTCTGATCGCCAACC	20	20	55.0	50.0	58.1	58.1	0.0	8.0	0.117323	65502	66076	1293-1301	1.000000	87.8
accD-psaI_p4	accD-psaI	4	GTATGTGCTTCTGGAGGAGC	GGATAATGGCCCAGCAGAAA	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.125033	65502	66076	1352-1360	1.000000	87.5
accD-psaI_p5	accD-psaI	5	GTATGTGCTTCTGGAGGAGC	AAAGGATAATGGCCCAGCAG	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.130942	65502	66076	1355-1363	1.000000	87.5
rpl33-rps18_p1	rpl33-rps18	1	TCTGTCCCTGTTGTTGCAAA	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	57.9	57.7	0.0	0.0	0.390844	75035	75279	381-476	1.000000	84.3
rpl33-rps18_p2	rpl33-rps18	2	TTCTGTCCCTGTTGTTGCAA	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	57.9	57.7	0.0	0.0	0.390844	75035	75279	382-477	1.000000	84.3
rpl33-rps18_p3	rpl33-rps18	3	ATTCTGTCCCTGTTGTTGCA	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	57.6	57.7	0.0	0.0	0.617852	75035	75279	383-478	1.000000	82.5
rpl33-rps18_p4	rpl33-rps18	4	TCTGTCCCTGTTGTTGCAAA	GGGCAAACGTCTACGAAAAG	20	20	45.0	50.0	57.9	57.4	0.0	9.9	0.725636	75035	75279	355-450	1.000000	82.1
rpl33-rps18_p5	rpl33-rps18	5	TTCTGTCCCTGTTGTTGCAA	GGGCAAACGTCTACGAAAAG	20	20	45.0	50.0	57.9	57.4	0.0	9.9	0.725636	75035	75279	356-451	1.000000	82.1
clpP_p1	clpP	1	CTAGTACATGTTCCTCGGCG	TGGACAGAAAGTAAACGGCC	20	20	55.0	50.0	57.9	58.1	2.3	0.0	0.234230	77225	79431	2748-2990	0.666667	76.7
clpP_p2	clpP	2	CTAGTACATGTTCCTCGGCG	AAACGGCCGGGATCATTTAA	20	20	55.0	45.0	57.9	57.9	2.3	31.8	0.252892	77225	79431	2736-2978	0.666667	76.6
clpP_p3	clpP	3	AGTCGCACATACACCCTAGT	AAACGGCCGGGATCATTTAA	20	20	50.0	45.0	58.2	57.9	0.0	31.8	0.283124	77225	79431	2751-2993	0.666667	76.3
clpP_p4	clpP	4	TGACATTTCGGATTGGCTGT	TGGACAGAAAGTAAACGGCC	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.311940	77225	79431	2696-2938	0.666667	76.2
clpP_p5	clpP	5	CTAGTACATGTTCCTCGGCG	ACCAAGGCAAACCCATGAAA	20	20	55.0	45.0	57.9	58.2	2.3	0.0	0.321183	77225	79431	2696-2938	0.666667	76.1
clpP-psbB_p1	clpP-psbB	1	TCGAATCCAAATCGCGATGT	ATACTCCAACCACCCCATGA	20	20	45.0	50.0	58.1	58.0	24.5	0.0	0.085602	79432	79939	1164-1412	1.000000	89.6
clpP-psbB_p2	clpP-psbB	2	TCGAATCCAAATCGCGATGT	TGATACTCCAACCACCCCAT	20	20	45.0	50.0	58.1	58.0	24.5	0.0	0.085602	79432	79939	1166-1414	1.000000	89.6
clpP-psbB_p3	clpP-psbB	3	TCGAATCCAAATCGCGATGT	ACCAAAACAAGCTACCCCTG	20	20	45.0	50.0	58.1	58.0	24.5	0.0	0.070035	79432	79939	1381-1629	1.000000	88.9
clpP-psbB_p4	clpP-psbB	4	TCGAATCCAAATCGCGATGT	ACACCCATATTCCAGGACCA	20	20	45.0	50.0	58.1	58.0	24.5	0.0	0.085602	79432	79939	1430-1678	1.000000	88.7
clpP-psbB_p5	clpP-psbB	5	TCGAATCCAAATCGCGATGT	ATCAAAACCTTCCACACCCC	20	20	45.0	50.0	58.1	58.0	24.5	0.0	0.075093	79432	79939	1498-1746	1.000000	88.6
rps3-rps19_p1	rps3-rps19	1	TACCAAGTCTGAAACCGAGC	TGATAAAAGATCTCGTCGTT	20	20	50.0	35.0	57.5	52.1	0.0	0.0	6.347507	91333	92062	565-792	1.000000	49.6
rps3-rps19_p2	rps3-rps19	2	GTCTGAAACCGAGCGGATTT	TGATAAAAGATCTCGTCGTT	20	20	50.0	35.0	58.6	52.1	16.8	0.0	6.457219	91333	92062	559-786	1.000000	49.6
rps3-rps19_p3	rps3-rps19	3	TACCAAGTCTGAAACCGAGC	AATGATAAAAGATCTCGTCGT	20	21	50.0	33.3	57.5	52.8	0.0	0.0	6.632944	91333	92062	567-794	1.000000	49.6
rps3-rps19_p4	rps3-rps19	4	TACCAAGTCTGAAACCGAGC	ATGATAAAAGATCTCGTCGTT	20	21	50.0	33.3	57.5	52.8	0.0	0.0	6.632944	91333	92062	566-793	1.000000	49.6
rps3-rps19_p5	rps3-rps19	5	GTCTGAAACCGAGCGGATTT	ATGATAAAAGATCTCGTCGTT	20	21	50.0	33.3	58.6	52.8	16.8	0.0	6.742655	91333	92062	560-787	1.000000	49.6
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	CCAGCGACGGTTTCATTAGA	20	20	50.0	50.0	58.0	58.0	7.6	0.0	0.013783	94425	94512	719-731	1.000000	83.4
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GTTGGGCGCTTTAACCATTC	TCCAGCGACGGTTTCATTAG	20	20	50.0	50.0	58.0	58.0	7.6	0.0	0.013783	94425	94512	720-732	1.000000	83.4
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	CACTTGACTGTCTACGGCTC	CCAGCGACGGTTTCATTAGA	20	20	55.0	50.0	58.0	58.0	9.5	0.0	0.020610	94425	94512	1293-1573	1.000000	83.2
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	CACTTGACTGTCTACGGCTC	TCCAGCGACGGTTTCATTAG	20	20	55.0	50.0	58.0	58.0	9.5	0.0	0.020610	94425	94512	1294-1574	1.000000	83.2
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	CCACAACGATGCTGTCCATA	CCAGCGACGGTTTCATTAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.027347	94425	94512	1340-1620	1.000000	83.0
ndhF-rpl32_p1	ndhF-rpl32	1	GGGGAATTGGGCAGACTTAG	AGCGTGTCTACCGATTTCAC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.066423	133894	134858	2184-2327	1.000000	87.0
ndhF-rpl32_p2	ndhF-rpl32	2	GGGGAATTGGGCAGACTTAG	CAGCGTGTCTACCGATTTCA	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.067794	133894	134858	2185-2328	1.000000	87.0
ndhF-rpl32_p3	ndhF-rpl32	3	AAATAACAGGAAGGCGCACA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.1	58.0	10.8	0.0	0.103543	133894	134858	2151-2294	1.000000	86.7
ndhF-rpl32_p4	ndhF-rpl32	4	AATAACAGGAAGGCGCACAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.1	58.0	10.8	0.0	0.103543	133894	134858	2150-2293	1.000000	86.7
ndhF-rpl32_p5	ndhF-rpl32	5	ATAACAGGAAGGCGCACAAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.1	58.0	10.8	0.0	0.103543	133894	134858	2149-2292	1.000000	86.7
rpl23_p1	rpl23	1	GAATGGTTAAAGCGCCCAAC	CACTTGACTGTCTACGGCTC	20	20	50.0	55.0	58.0	58.0	2.3	9.5	0.025228	174169	174468	588-588	0.333333	53.1
rpl23_p2	rpl23	2	GAATGGTTAAAGCGCCCAAC	CCACAACGATGCTGTCCATA	20	20	50.0	50.0	58.0	58.0	2.3	0.0	0.031965	174169	174468	635-635	0.333333	53.1
rpl23_p3	rpl23	3	GAATGGTTAAAGCGCCCAAC	TCTGTCGGTGCTTGAAACAA	20	20	50.0	45.0	58.0	58.0	2.3	0.0	0.040459	174169	174468	1143-1143	0.333333	53.0
rpl23_p4	rpl23	4	GAATGGTTAAAGCGCCCAAC	TTCTGTCGGTGCTTGAAACA	20	20	50.0	45.0	58.0	58.0	2.3	0.0	0.040459	174169	174468	1144-1144	0.333333	53.0
rpl23_p5	rpl23	5	GTTGAACCCTGTGAATTGCG	CACTTGACTGTCTACGGCTC	20	20	50.0	55.0	57.9	58.0	0.0	9.5	0.066505	174169	174468	897-897	0.333333	52.8